Abstract: This study was conducted to formulate diclofenac sodium-loaded chitosan nanoparticles and to study the effect of formulation compositions on particle size and zeta potential of chitosan nanoparticles (CSN) containing diclofenac sodium (DC) prepared by ionotropic gelation method. It was found that the formulations containing chitosan, DC and tripolyphosphate (TPP) at a weight ratio of 4:1:1, respectively, with various pH provided various systems. At pH 5.0 and 6.0, the obtained systems were turbid because of precipitation of DC and chitosan, respectively. However, the dispersed system of CSN possessing diameter of 108±1 nm and zeta potential of 19±1 mV could be obtained at pH 5.5. These CSN also showed spherical morphology observed via a transmission scanning electron microscope. Change in weight ratio of chitosan:DC:TPP i.e. 1:1:1, 2:1:1, 3:1:1 and 4:1:1 showed that these ratios led to precipitation of particles except for the ratio of 4:1:1 providing CSN properly. The effect of Tween 80 as a stabilizer was also determined. It suggested that increment of Tween 80 concentration to 0.02% w/v could stabilize CSN at least 48 hours. However, increment of Tween 80 to 0.03% w/v led to quick precipitation of particles. The study of effect of TPP suggested that increment of TPP concentration increased particle size but decreased zeta potential. The excess TPP caused precipitation of CSN. Therefore, the optimized CSN was the CSN containing chitosan, DC and TPP at the ratio of 4:1:1and 0.02% w/v Tween 80 prepared at pH 5.5. Their particle size, zeta potential and entrapment efficiency were 128±1 nm, 15±1 mV and 45.8±2.6%, respectively.
Abstract: No negative control nor control to prevent microbes from escaping was set when the S-shaped flask experiments were performed by Pasteur. Microscope was not used to observe the media in the flasks. Louis Pasteur’s S-shaped flask experiment was re-examined by using U-shaped flasks, modified S-shaped flasks and microscope. A mixture of microbes was isolated from the room air, from which one rod-shaped Bacillus species with proposed name Bacillus gaso-mobilis sp nov and one grape-shaped Staphylococcus species with proposed name of Staphylococcus gaso-mobilis sp nov were identified. Their penicillin and ampicillin resistant strains containing plasmids were isolated. These bacteria could change color, produce odor and automatically move in the air. They did not form colonies on solid media. They had a high suspension capacity in liquid media. Their light absorbance peaked at the wave length of 320 nm. It was concluded that there were flaws with Louis Pasteur’s S-shaped flask experiments.
Abstract: Microorganisms can be removed, inhibited or killed by physical agents, physical processes or chemical agents but they have their inherent disadvantages such as increased resistance against antibiotics etc. Since, plants have endless ability to synthesize aromatic substances which act as the master agents for plant defense mechanisms against microorganisms, insects and herbivores. Thus, secondary metabolites or phytochemicals obtained from plants can be used as agents of disease control nowadays. In the present study effect of different concentrations of acetone fraction of leaves and alcohol fraction of inflorescence of Euphorbia pulcherrima on various cytomorphological parameters i.e. cell number, mycelium width, conidial size, conidiophore size etc. of Aspergillus fumigatus has been studied. Change in mycelium/ hyphal cell width, conidium size, conidiophore size etc. was measured with the help of a previously calibrated oculometer. To study effect on morphology, fungal mycelium along with conidiophore and conidia were stained with cotton blue and mounted in lactophenol and observed microscopically. Inhibitory action of the acetone extract of Euphorbia pulcherrima leaf on growth of Aspergillus fumigatus was investigated. Control containing extract free medium supported profuse growth of the fungus. Although decrease in growth was observed even at 3.95μg/ml but significant inhibition of growth was started at7.81μg/ml concentration of the extract. Complete inhibition was observed at 15.62μg/ml and above. Microscopic examination revealed that at 3.95, 7.81 and 15.62μg/ml extract concentration hyphal cell width was found to be increased from 1.44μm in control to 3.86, 5.24 and 8.98 μm respectively giving a beaded appearance to the mycelium. Vesicle size was reduced from 24.78x20.08μm (control) to 11.34x10.06μm at 3.95μg/ml concentration. At 7.81 and 15.62μg/ml concentration no phialides and sterigmata were observed. Inhibitory action of the alcohol extract of inflorescence on the growth of Aspergillus fumigatus was also studied. Control containing extract free medium supported profuse growth of the fungus. Although decrease in growth was observed even at 3.95μg/ml but complete inhibition was observed at 62.5μg/ml and above. Microscopic examination revealed that hyphal cell width of Aspergillus fumigatus was found to be increased from 1.67μm in control to 5.84μm at MIC i.e. at 62.5μg/ml. Vesicle size was reduced from 44.76x 24.22μm (control) to 11.36x 6.80μm at 15.62μg/ml concentrations. At 31.25 μg/ml and 62.5μg/ml concentration no phialides and sterigmata was found. Spore germination was completely found to be inhibited at 3.95μg/ml concentration. Similarly 92.87% reduction in vesicle size was observed at 15.62μg/ml concentration. It is evident from the results that plant extracts inhibit fungal growth and this inhibition is concentration dependent.
Abstract: It can be determined in preference between
representative mechanical and mathematical model of elasticcreeping
deformation of transversally isotropic array with doubly
periodic system of tilted slots, and offer of the finite elements
calculation scheme, and inspection of the states of two diagonal
arbitrary profile cavities of deep inception, and in setting up the tense
and dislocation fields distribution nature in computing processes.
Abstract: Purpose: To compare attitudes and perceptions of
Israeli native born mothers versus former Soviet Union (FSU)
immigrant mothers regarding the physical health of their infant.
Methodology: cross-sectional design. A convenience sample of 50
participants was recruited by face to face and snowball technique. A
questionnaire was constructed according to the instructions of the
Ministry of Health for the care and treatment of infants. The main
areas explored were: sources of knowledge that the young mother
acquired regarding the care of her infant, ways of caring for the
infant, hygiene and sanitary habits, and the pattern of referral to
health professionals. The last topic relates to emotions mothers might
experience towards their infant.
Results: Mothers from both cultural groups present some similar
caring behaviors, which may express a universal aspect of mothers'
behavior towards their infants. However, immigrant mothers differ
significantly from native born by relying less on their mothers' and
grandmothers' experience, they wean their infants from diapers
earlier, they are stricter about hygiene and sanitary habits and they
tend to consult a physician when their infant has low fever. Native
born and immigrant mothers differ in their expressions of pride and
wonder. Immigrant mothers report of a lesser degree of these
emotions towards their infants than native born mothers.
Conclusion: The theoretical model of socialization and
acculturation of immigrant mothers is employed as an explanatory
model for the current findings Young immigrant mothers undergo a
complex acculturation process and adapt behavioral patterns in
various areas to comply with Israeli norms and values, demonstrating
assimilation. In other areas they adhere to the norms of their original
culture.
Abstract: The adsorption of bovine serum albumin (BSA), immunoglobulin G (IgG) and fibrinogen (Fgn) on fluorinated selfassembled monolayers have been studied using time of flight secondary ion mass spectrometry (ToF-SIMS) and Spectroscopic Ellipsometry (SE). The objective of the work has to establish the utility of ToF-SIMS for the determination of the amount of protein adsorbed on the surface. Quantification of surface adsorbed proteins was carried out using SE and a good correlation between ToF-SIMS results and SE was achieved. The surface distribution of proteins were also analysed using Atomic Force Microscopy (AFM). We show that the surface distribution of proteins strongly affect the ToFSIMS results.
Abstract: In order to integrate knowledge in heterogeneous
case-based reasoning (CBR) systems, ontology-based CBR system
has become a hot topic. To solve the facing problems of
ontology-based CBR system, for example, its architecture is
nonstandard, reusing knowledge in legacy CBR is deficient, ontology
construction is difficult, etc, we propose a novel approach for
semi-automatically construct ontology-based CBR system whose
architecture is based on two-layer ontology. Domain knowledge
implied in legacy case bases can be mapped from relational database
schema and knowledge items to relevant OWL local ontology
automatically by a mapping algorithm with low time-complexity. By
concept clustering based on formal concept analysis, computing
concept equation measure and concept inclusion measure, some
suggestions about enriching or amending concept hierarchy of OWL
local ontologies are made automatically that can aid designers to
achieve semi-automatic construction of OWL domain ontology.
Validation of the approach is done by an application example.
Abstract: For the improvement of the ability in detecting
small calcifications using Ultrasonography (US) we propose a
novel indicator of calcifications in an ultrasound B-mode image
without decrease in frame rate. Since the waveform of an
ultrasound pulse changes at a calcification position, the
decorrelation of adjacent scan lines occurs behind a
calcification. Therefore, we employ the decorrelation of
adjacent scan lines as an indicator of a calcification. The
proposed indicator depicted wires 0.05 mm in diameter at 2 cm
depth with a sensitivity of 86.7% and a specificity of 100%,
which were hardly detected in ultrasound B-mode images. This
study shows the potential of the proposed indicator to
approximate the detectable calcification size using an US
device to that of an X-ray imager, implying the possibility that
an US device will become a convenient, safe, and principal
clinical tool for the screening of breast cancer.
Abstract: The benefits of eco-roofs is quite well known, however there remains very little research conducted for the implementation of eco-roofs in subtropical climates such as Australia. There are many challenges facing Australia as it moves into the future, climate change is proving to be one of the leading challenges. In order to move forward with the mitigation of climate change, the impacts of rapid urbanization need to be offset. Eco-roofs are one way to achieve this; this study presents the energy savings and environmental benefits of the implementation of eco-roofs in subtropical climates. An experimental set-up was installed at Rockhampton campus of Central Queensland University, where two shipping containers were converted into small offices, one with an eco-roof and one without. These were used for temperature, humidity and energy consumption data collection. In addition, a computational model was developed using Design Builder software (state-of-the-art building energy simulation software) for simulating energy consumption of shipping containers and environmental parameters, this was done to allow comparison between simulated and real world data. This study found that eco-roofs are very effective in subtropical climates and provide energy saving of about 13% which agrees well with simulated results.
Abstract: Utilizing echoic intension and distribution from different organs and local details of human body, ultrasonic image can catch important medical pathological changes, which unfortunately may be affected by ultrasonic speckle noise. A feature preserving ultrasonic image denoising and edge enhancement scheme is put forth, which includes two terms: anisotropic diffusion and edge enhancement, controlled by the optimum smoothing time. In this scheme, the anisotropic diffusion is governed by the local coordinate transformation and the first and the second order normal derivatives of the image, while the edge enhancement is done by the hyperbolic tangent function. Experiments on real ultrasonic images indicate effective preservation of edges, local details and ultrasonic echoic bright strips on denoising by our scheme.
Abstract: Certain sciences such as physics, chemistry or biology,
have a strong computational aspect and use computing infrastructures
to advance their scientific goals. Often, high performance and/or high
throughput computing infrastructures such as clusters and computational
Grids are applied to satisfy computational needs. In addition,
these sciences are sometimes characterised by scientific collaborations
requiring resource sharing which is typically provided by Grid
approaches. In this article, I discuss Grid computing approaches in
High Energy Physics as well as in bioinformatics and highlight some
of my experience in both scientific domains.
Abstract: Mostly, pedestrian-car accidents occurred at a
signalized interaction is because pedestrians cannot across the
intersection safely within the green light. From the viewpoint of
pedestrian, there might have two reasons. The first one is pedestrians
cannot speed up to across the intersection, such as the elders. The other
reason is pedestrians do not sense that the signal phase is going to
change and their right-of-way is going to lose. Developing signal logic
to protect pedestrian, who is crossing an intersection is the first
purpose of this study. Another purpose of this study is improving the
reliability and reduce delay of public transportation service. Therefore,
bus preemption is also considered in the designed signal logic. In this
study, the traffic data of the intersection of Chong-Qing North Road
and Min-Zu West Road, Taipei, Taiwan, is employed to calibrate and
validate the signal logic by simulation. VISSIM 5.20, which is a
microscopic traffic simulation software, is employed to simulate the
signal logic. From the simulated results, the signal logic presented in
this study can protect pedestrians crossing the intersection
successfully. The design of bus preemption can reduce the average
delay. However, the pedestrian safety and bus preemptive signal will
influence the average delay of cars largely. Thus, whether applying the
pedestrian safety and bus preemption signal logic to an isolated
intersection or not should be evaluated carefully.
Abstract: Heterogeneous repolarization causes dispersion of the T-wave and has been linked to arrhythmogenesis. Such heterogeneities appear due to differential expression of ionic currents in different regions of the heart, both in healthy and diseased animals and humans. Mice are important animals for the study of heart diseases because of the ability to create transgenic animals. We used our previously reported model of mouse ventricular myocytes to develop 2D mouse ventricular tissue model consisting of 14,000 cells (apical or septal ventricular myocytes) and to study the stability of action potential propagation and Ca2+ dynamics. The 2D tissue model was implemented as a FORTRAN program code for highperformance multiprocessor computers that runs on 36 processors. Our tissue model is able to simulate heterogeneities not only in action potential repolarization, but also heterogeneities in intracellular Ca2+ transients. The multicellular model reproduced experimentally observed velocities of action potential propagation and demonstrated the importance of incorporation of realistic Ca2+ dynamics for action potential propagation. The simulations show that relatively sharp gradients of repolarization are predicted to exist in 2D mouse tissue models, and they are primarily determined by the cellular properties of ventricular myocytes. Abrupt local gradients of channel expression can cause alternans at longer pacing basic cycle lengths than gradual changes, and development of alternans depends on the site of stimulation.
Abstract: Research on the boron (B) toxicity problems had recently considerable relation, especially in the dry regions of the world. Development of resistant varieties to B toxicity is a high priority on these regions, where the soils have high levels of B. Thus, this study aimed to assessment the resistance of wheat genotypes to B toxicity using the agronomic and physiologic parameters. For this aim, a pot experiment, based on a completely randomized design with three replications, was conducted using the soil of calcareous usthochrepts. In the study, twenty different wheat genotypes of T. aestivum and T. Durum were used. Boron fertilizer at the levels of 0 (-B), 30 mg B kg-1 (+B) as H3BO3 was applied to the pots. After harvest, plant dry matter yield was recorded, and total B concentrations in tops of wheat plants were determined. The results have revealed the existence of a large genotypic variation among wheat genotypes to their physiologic and agronomic susceptibility to B toxicity.
Abstract: Over the early years of the 21st century, cities
throughout the Middle East, particularly in the Gulf region have
expanded more rapidly than ever before. Given the presence of a
large volume of high-rise buildings allover the region, the local
authority aims to set a new standard for sustainable development;
with an integrated approach to maintain a balance between economy,
quality, environmental protection and safety of life. In the very near
future, as mandatory requirements, sustainability will be the criteria
that should be included in all building projects. It is well known in
the building sustainability topics that structural design engineers do
not have a key role in this matter. In addition, the LEED (Leadership
in Energy and Environmental Design) has looked almost exclusively
on the environmental components and materials specifications. The
objective of this paper is to focus and establish groundwork for
sustainability techniques and applications related to the RC high-rise
buildings design, from the structural point of view. A set of
recommendations related to local conditions, structural modeling and
analysis is given, and some helpful suggestions for structural design
team work are addressed. This paper attempts to help structural
engineers in identifying the building sustainability design, in order to
meet local needs and achieve alternative solutions at an early stage of
project design.
Abstract: In this article, we are dealing with a model consisting of a classical Van der Pol oscillator coupled gyroscopically to a linear oscillator. The major problem is analyzed. The regular dynamics of the system is considered using analytical methods. In this case, we provide an approximate solution for this system using parameter-expansion method. Also, we find approximate values for frequencies of the system. In parameter-expansion method the solution and unknown frequency of oscillation are expanded in a series by a bookkeeping parameter. By imposing the non-secularity condition at each order in the expansion the method provides different approximations to both the solution and the frequency of oscillation. One iteration step provides an approximate solution which is valid for the whole solution domain.
Abstract: Electron back-scattered diffraction was used to follow the evolution of microstructure from the base metal to the stir zone (SZ) in a duplex stainless steel subjected to friction stir welding. In the stir zone (SZ), a continuous dynamic recrystallization (CDRX) was evidenced for ferrite, while it was suggested that a static recrystallization together with CDRX may occur for austenite. It was found that ferrite and austenite grains in the SZ take a typical shear texture of bcc and fcc materials respectively.
Abstract: Debates on residential satisfaction topic have been
vigorously discussed in family house setting. Nonetheless, less or
lack of attention was given to survey on student residential
satisfaction in the campus house setting. This study, however, tried to
fill in the gap by focusing more on the relationship between students-
socio-economic backgrounds and student residential satisfaction with
their on-campus student housing facilities. Two-stage cluster
sampling method was employed to classify the respondents. Then,
self-administered questionnaires were distributed face-to-face to the
students. In general, it was confirmed that the students- socioeconomic
backgrounds have significantly influence the students-
satisfaction with their on-campus student housing facilities. The main
influential factors were revealed as the economic status, sense of
sharing, and the ethnicity of roommates. Likewise, this study could
also provide some useful feedback for the universities administration
in order to improve their student housing facilities.
Abstract: Microscopic emission and fuel consumption models
have been widely recognized as an effective method to quantify real
traffic emission and energy consumption when they are applied with
microscopic traffic simulation models. This paper presents a
framework for developing the Microscopic Emission (HC, CO, NOx,
and CO2) and Fuel consumption (MEF) models for light-duty
vehicles. The variable of composite acceleration is introduced into
the MEF model with the purpose of capturing the effects of historical
accelerations interacting with current speed on emission and fuel
consumption. The MEF model is calibrated by multivariate
least-squares method for two types of light-duty vehicle using
on-board data collected in Beijing, China by a Portable Emission
Measurement System (PEMS). The instantaneous validation results
shows the MEF model performs better with lower Mean Absolute
Percentage Error (MAPE) compared to other two models. Moreover,
the aggregate validation results tells the MEF model produces
reasonable estimations compared to actual measurements with
prediction errors within 12%, 10%, 19%, and 9% for HC, CO, NOx
emissions and fuel consumption, respectively.
Abstract: The main objective of this paper is to identify and
disseminate good practice in quality assurance and enhancement as
well as in teaching and learning at master level. This paper focuses
on the experience of the Erasmus Mundus Master program CIMET
(Color in Informatics and Media Technology). Amongst topics
covered, we discuss the adjustments necessary to a curriculum
designed for excellent international students and their preparation for
a global labor market.