Abstract: A total of 6 isolates of Bacillus subtilis were isolated from oil mill waste collected in Namakkal district, Tamilnadu, India. The isolated bacteria were screened using lipase screening medium containing Tween 80. BS-3 isolate exhibited a greater clear zone than the others, indicating higher lipase activity. Therefore, this isolate was selected for media optimization studies. Ten process variables were screened using Plackett–Burman design and were further optimized by central composite design of response surface methodology for lipase production in submerged fermentation. Maximum lipase production of 16.627 U/min/ml were predicted in medium containing yeast extract (9.3636g), CaCl2 (0.8986g) and incubation periods (1.813 days). A mean value of 16.98 ± 0.2286 U/min/ml of lipase was acquired from real experiments.
Abstract: Changes in the sensory, chemical and microbiological quality of the Mediterranean hake during post-catch handling and distribution were investigated. 115 fish samples were seasonally received during three stages of the transfer route from the sea to the consumer and two storage methods were recorded, seawater and ice storage. Microbiological evaluation revealed higher status for the ice stored samples regarding heterotrophic bacteria (2.68 log cfu/g and 1.92 log cfu/g at 22oC and 37°C respectively) and psychrotrophic counts (3.20 log cfu/g), with statistically significant differences among storage methods. Sensory evaluation also revealed higher status for the ice stored samples with a mean quality index of 0.17 and a spoilage time estimated at 30 hours, in contrast to seawater storage, which varied from 0.28 to 0.3, and a 14-hour estimated spoilage. Detected pathogens were identified mainly in the seawater stored samples, posing questions on the quality of the product reaching the seafood markets.
Abstract: Considering the increasing need of biofuels in Europe and the legislative requirements of the European Union it is needed to quantify the greenhouse gas emissions of biofuels life cycle. In this article a carbon footprint analysis to quantify these gases emitted during production and use of Romanian rapeseed oil (RO) and biodiesel from rapeseed oil (RME) was conducted. The functional unit was considered the LHV of diesel oil of 42.8 MJ·kg-1 corresponding to 1.15kg. of RO and 1.10 kg. of RME. When the 3 fuels were compared, the results show important benefits when using rapeseed oil or biodiesel instead of diesel. The most impacting stage in terms of GHG emissions is the use of the fuels. In this stage, rapeseed oil registers a total quantity of 3,229 kg CO2eq.·FU-1 and biodiesel register a total quantity of 3,088 kg CO2eq.·FU-1 while mineral diesel registers a total quantity of 3,156 kg CO2eq.·FU-1 emitted in the air. Taking into account that rape plant absorbed during growth stage the same quantity of CO2 as emitted into atmosphere during usage stage of the fuel, when compared the three fuels, rapeseed oil and biodiesel obtain obvious benefits against fossil diesel. Results show that by substituting diesel with RO a total quantity of 5,663 kg. CO2eq.·FU-1 would be saved while using biodiesel a total quantity of 5,570 kg. CO2eq.·FU-1 can be saved.
Abstract: This paper deals with design of walking beam pendel
axle suspension system. This axles and suspension systems are
mainly required for transportation of heavy duty and Over Dimension
Consignment (ODC) cargo, which is exceeding legal limit in terms of
length, width and height. Presently, in Indian transportation industry,
ODC movement growth rate has increased in transportation of bridge
sections (pre-cast beams), transformers, heavy machineries, boilers,
gas turbines, windmill blades etc. However, current Indian standard
road transport vehicles are facing lot of service and maintenance
issues due to non availability of suitable axle and suspension to carry
the ODC cargoes. This in turn will lead to increased number of road
accidents, bridge collapse and delayed deliveries, which finally result
in higher operating cost. Understanding these requirements, this work
was carried out. These axles and suspensions are designed for
optimum self – weight with maximum payload carrying capacity with
better road stability.
Abstract: Recently, a growing interest has emerged on the development of new and efficient energy sources, due to the inevitable extinction of the nonrenewable energy reserves. One of these alternative sources which have a great potential and sustainability to meet up the energy demand is biomass energy. This significant energy source can be utilized with various energy conversion technologies, one of which is biomass gasification in supercritical water.
Water, being the most important solvent in nature, has very important characteristics as a reaction solvent under supercritical circumstances. At temperatures above its critical point (374.8oC and 22.1MPa), water becomes more acidic and its diffusivity increases. Working with water at high temperatures increases the thermal reaction rate, which in consequence leads to a better dissolving of the organic matters and a fast reaction with oxygen. Hence, supercritical water offers a control mechanism depending on solubility, excellent transport properties based on its high diffusion ability and new reaction possibilities for hydrolysis or oxidation.
In this study the gasification of a real biomass, namely olive mill wastewater (OMW), in supercritical water conditions is investigated with the use of Ru/Al2O3 catalyst. OMW is a by-product obtained during olive oil production, which has a complex nature characterized by a high content of organic compounds and polyphenols. These properties impose OMW a significant pollution potential, but at the same time, the high content of organics makes OMW a desirable biomass candidate for energy production.
The catalytic gasification experiments were made with five different reaction temperatures (400, 450, 500, 550 and 600°C) and five reaction times (30, 60, 90, 120 and 150s), under a constant pressure of 25MPa. Through these experiments, the effects of reaction temperature and time on the gasification yield, gaseous product composition and OMW treatment efficiency were investigated.
Abstract: Synthetic oily wastewaters were prepared from metal working fluids (MWF). Electrocoagulation experiments were performed under constant voltage application. The current, conductivity, pH, dissolved oxygen concentration and temperature were recorded on line at every 5 seconds during the experiments. Effects of applied voltage differences, electrode materials and distance between electrodes on removal efficiency have been investigated. According to the experimental results, the treatment of MWF wastewaters by iron electrodes rather than aluminum and stainless steel was much quicker; and the distance between electrodes should be less than 1cm. The electrocoagulation process was modeled by using block oriented approach and found out that it can be modeled as a single input and multiple output system. Modeling studies indicates that the electrocoagulation process has a nonlinear model structure.
Abstract: The banking sector poses a lot of problems in Nigeria in general and the non-oil export sector in particular. The banks' lack effectiveness in handling small, medium or long-term credit risk (lack of training of loan officers, lack of information on borrowers and absence of a reliable credit registry) results in non-oil exporters being burdened with high requirements, such as up to three years of financial statements, enough collateral to cover both the loan principal and interest (including a cash deposit that may be up to 30% of the loans' net present value), and to provide every detail of the international trade transaction in question. The stated problems triggered this research. Consequently, information on bank financing of non-oil exports was collected from 100 respondents from the 20 Deposit Money Banks (DMBs) in Nigeria. The data was analysed by the use of descriptive statistics correlation and regression. It is found that, Nigerian banks are participants in the financing of non-oil exports. Despite their participation, the rate of interest for credit extended to non-oil export is usually high, ranging between 15-20%. Small and medium sized non-oil export businesses lack the credit history for banks to judge them as reputable. Banks also consider the non-oil export sector very risky for investment. The banks actually do grant less credit than the exporters may require and therefore are not properly funded by banks. Banks grant very low volume of foreign currency loan in addition to, unfavorable exchange rate at which Naira is exchanged to the Dollar and other currencies in the country. This makes importation of inputs costly and negatively impacted on the non-oil export performance in Nigeria.
Abstract: The understanding on the contribution of root respiration to total soil respiration is still very limited, especially for sugarcane. In this study, trenching experiments in sugarcane plantations were conducted to separate and investigate soil respiration for this crop. The measurements were performed for the whole growing period of 344 days to quantify root respiration. The obtained monitoring data showed that the respiration rate is increasing with the age of the plant, accounting for up to 29% of the total soil respiration before harvesting. The root to soil respiration ratio increased rapidly during the young seedling stage, i.e. first five months, then declined and finally got stabilized during yield formation and ripening stages, respectively. In addition, the results from the measurements confirmed that soil respiration was positively correlated with soil moisture content.
Abstract: This study focused on the estimation of carbon released to the atmosphere from dry dipterocarp forest (DDF) fires in Thailand. Laboratory experiments were conducted using a cone calorimeter to simulate the DDF fires. The leaf litter collected from DDF in western Thailand was used as biomass fuel. Three different masses of leaf litter were employed, 7g, 10g and 13g, to estimate the carbon released from this type of vegetation fire to the atmosphere. The chemical analysis of the leaf litter showed that the carbon content in the experimental biomass fuel was 46.0±0.1%. From the experiments, it was found that more than 95% of the carbon input was converted to carbon released to the atmosphere, while less than 5% were left in the form of residues, and returned to soil. From the study, the carbon released amounted 440.213±2.243 g/kgdry biomass, and the carbon retained in the residues was 19.786±2.243 g/kgdry biomass. The quantity of biomass fuel consumed to produce 1 g of carbon released was 2.27±0.01gkgdry biomass. Using these experimental data of carbon produced by the DDF fires, it was estimated that this type of fires in 2009 contributed to 4.659 tonnes of carbon released to the atmosphere, and 0.229 tonnes of carbon in the residues to be returned to soil in Thailand.
Abstract: Field study was conducted to determine the post field soil fertility status responses of pawpaw (Carica papaya L.) var. homestead selection and sunrise-solo orchards to organo-mineral fertilizer (OMF) rates applied at 10, 20 40 t/ha where both the zero t/ha OMF and NPK 15:15:15 at 50 g/plant/month served as control. The result showed that all pawpaw orchards treated with OMF rates recorded significantly (p≤0.01) higher % P, % K, Na and % organic matter in soil compared to applied NPK which recorded lower Na. However, while orchards plated with sole pawpaw were higher in soil bulk density (SBD), orchards with homestead mixture were lower in SBD and significantly lower % organic matter compared to obtainable under sunrise crop mixture which recorded lower Na and Mg. In conclusion, as a result of loosening effect on soil particles, the homestead pawpaw probably due to more rooting activities as well as the addition of organic fertilizer to soils both had significant influence leading to lower SBD.
Abstract: According to synthetic plastics obtained from petroleum cause some environmental problems. Therefore, degradable plastics become widely used and studied for replacing the synthetic plastic waste. A biopolymer of poly hydroxybutyrate-co-hydroxyvalerate (PHBV) is subgroups of a main kind of polyhydroxyalkanoates (PHAs). Naturally, PHBV is hard, brittle and low flexible while natural rubber (NR) is high elastic latex. Then, they are blended and the biodegradation of the blended PHBV and NR films were examined in soil environment. The results showed that the degradation occurs predominantly in the bulk of the samples. The order of biodegradability was shown as follows: PHBV> PHBV/NR> NR. After biodegradation, the blended films were characterized by appearance analysis such as Scanning Electron Microscope (SEM), Fourier transform infrared spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC). It was found that the biodegradation mainly occurred at the polymer surface.
Abstract: The objective of this study is to investigate the performance of the electrocoagulation process for color and COD removal in palm oil wastewater using a 10 L batch reactor. Iron was used as electrodes and the distance between electrodes was 2 cm. The effects of operating parameters: current voltage (6, 12 and 18 volt), reaction time (5, 15, 30, 45 and 60 min) and initial pH (4 and 9) of treatment efficiency were examine. The result showed that decolorization and COD removal efficiency increased with the increase in current voltage and reaction time. The proper condition for decolorization achieved at initial pH 4 and 9 were current voltage of 12 volt, reaction time 30 min. The decolorization efficiency reached 90.4% and 88.9%, respectively. COD removal was achiveved at current voltage 12 volt, reaction time 15 min. COD removal efficiency was 89.2 % and 83.0%, respectively. From the results, to show electrocoagulation process can treat palm oil mill wastewater in both acidic and basic condition at high efficiency for color and COD removal. Consequently, electrocoagulation process can be used or applied as a post-treatment step to improve the quality of the final discharge in term of color and residual COD removal.
Abstract: Vetiver oil is secondary metabolite that accumulates in Vetiveria zizanioides roots. The aim of this study was to obtain best type of root culture which produce high amount of vetiver oil, and was similar to metabolites produce from its mother plant. Protein analysis was also conducted to detect protein, related to putative enzymes, which have a role in terpenoids synthesis in the root culture. The results showed that root culture derived from crown explant produced the best root growth. The root culture produced primary and lateral roots, ca. 40 branches. The vetiver oil produced from root culture was analyzed by using GC-MS., and the highest content of terpenoids from roots of crown explant attained 19.024%. The result of SDS PAGE showed proteins which were ±61 kD and ± 68 kD, each might be related to putative monoterpene synthase and sesquiterpenes complex, respectively.
Abstract: Different vegetable oil based biodiesel (FAMES) were prepared by alkaline transesterification using refined oils as well as waste frying oil (WFO). Methanol and sodium hydroxide are used as catalyst under similar reaction conditions. To ensure the quality of biodiesel produced, a series of different ASTM Standard tests were carried out. In this context, various testwere done including viscosity, carbon residue, specific gravity, corrosion test, flash point, cloud point and pour point. Results revealed that characteristics of biodiesel depend on the feedstock and it is far better than petroleum diesel.
Abstract: In biomedical implant field, a new formula is given
for the study of Radio Frequency power attenuation by simultaneous
effects of side and angular misalignment of the supply/data transfer
coils. A confrontation with the practical measurements done into a
Faraday cage, allowed a checking of the obtained theoretical results.
The DC supply systems without material connection and the data
transmitters used in the case of biomedical implants, can be well
dimensioned by taking into account the possibility of power
attenuation by misalignment of transfer coils
Abstract: Different approaches for heating\cooling of stirred tanks, coils and jackets, are investigated using computational fluid dynamics (CFD).A time-dependant sliding mesh approach is applied to simulate the flow in both conditions. The investigations are carried out under the turbulent flow conditions for a Rushton impeller and heating elements are considered isothermal. The flow behavior and temperature distribution are studied for each case and heat transfer coefficient is calculated. Results show different velocity profiles for each case. Unsteady temperature distribution is not similar for different cases .In the case of the coiled stirred vessel more uniform temperature and higher heat transfer coefficient is resulted.
Abstract: Themain goal of this article is to find efficient
methods for elemental and molecular analysis of living
microorganisms (algae) under defined environmental conditions and
cultivation processes. The overall knowledge of chemical
composition is obtained utilizing laser-based techniques, Laser-
Induced Breakdown Spectroscopy (LIBS) for acquiring information
about elemental composition and Raman Spectroscopy for gaining
molecular information, respectively. Algal cells were suspended in
liquid media and characterized using their spectra. Results obtained
employing LIBS and Raman Spectroscopy techniques will help to
elucidate algae biology (nutrition dynamics depending on cultivation
conditions) and to identify algal strains, which have the potential for
applications in metal-ion absorption (bioremediation) and biofuel
industry. Moreover, bioremediation can be readily combined with
production of 3rd generation biofuels. In order to use algae for
efficient fuel production, the optimal cultivation parameters have to
be determinedleading to high production of oil in selected
cellswithout significant inhibition of the photosynthetic activity and
the culture growth rate, e.g. it is necessary to distinguish conditions
for algal strain containing high amount of higher unsaturated fatty
acids. Measurements employing LIBS and Raman Spectroscopy were
utilized in order to give information about alga Trachydiscusminutus
with emphasis on the amount of the lipid content inside the algal cell
and the ability of algae to withdraw nutrients from its environment
and bioremediation (elemental composition), respectively. This
article can serve as the reference for further efforts in describing
complete chemical composition of algal samples employing laserablation
techniques.
Abstract: In order to Study the efficacy application of green
manure as chickpea pre plant, field experiments were carried out in
2007 and 2008 growing seasons. In this research the effects of
different strategies for soil fertilization were investigated on grain
yield and yield component, minerals, organic compounds and
cooking time of chickpea. Experimental units were arranged in splitsplit
plots based on randomized complete blocks with three
replications. Main plots consisted of (G1): establishing a mixed
vegetation of Vicia panunica and Hordeum vulgare and (G2):
control, as green manure levels. Also, five strategies for obtaining the
base fertilizer requirement including (N1): 20 t.ha-1 farmyard manure;
(N2): 10 t.ha-1 compost; (N3): 75 kg.ha-1 triple super phosphate;
(N4): 10 t.ha-1 farmyard manure + 5 t.ha-1 compost and (N5): 10 t.ha-1
farmyard manure + 5 t.ha-1 compost + 50 kg.ha-1 triple super
phosphate were considered in sub plots. Furthermoree four levels of
biofertilizers consisted of (B1): Bacillus lentus + Pseudomonas
putida; (B2): Trichoderma harzianum; (B3): Bacillus lentus +
Pseudomonas putida + Trichoderma harzianum; and (B4): control
(without biofertilizers) were arranged in sub-sub plots. Results
showed that integrating biofertilizers (B3) and green manure (G1)
produced the highest grain yield. The highest amounts of yield were
obtained in G1×N5 interaction. Comparison of all 2-way and 3-way
interactions showed that G1N5B3 was determined as the superior
treatment. Significant increasing of N, P2O5, K2O, Fe and Mg content
in leaves and grains emphasized on superiority of mentioned
treatment because each one of these nutrients has an approved role in
chlorophyll synthesis and photosynthesis abilities of the crops. The
combined application of compost, farmyard manure and chemical
phosphorus (N5) in addition to having the highest yield, had the best
grain quality due to high protein, starch and total sugar contents, low
crude fiber and reduced cooking time.
Abstract: Research on the boron (B) toxicity problems had recently considerable relation, especially in the dry regions of the world. Development of resistant varieties to B toxicity is a high priority on these regions, where the soils have high levels of B. Thus, this study aimed to assessment the resistance of wheat genotypes to B toxicity using the agronomic and physiologic parameters. For this aim, a pot experiment, based on a completely randomized design with three replications, was conducted using the soil of calcareous usthochrepts. In the study, twenty different wheat genotypes of T. aestivum and T. Durum were used. Boron fertilizer at the levels of 0 (-B), 30 mg B kg-1 (+B) as H3BO3 was applied to the pots. After harvest, plant dry matter yield was recorded, and total B concentrations in tops of wheat plants were determined. The results have revealed the existence of a large genotypic variation among wheat genotypes to their physiologic and agronomic susceptibility to B toxicity.
Abstract: The objective of this study was to determine the
effects of garlic oil (Allium sativa), turmeric powder (Curcuma longa
Linn) and Monensin on Total apparent digestibility of nutrients in
Baloochi lambs. The experiment was designed as a 4 x 4 Latin square
using 4 ruminally baloochi lambs with 4 treatments in four 28-d
periods. Treatments were control (no additive), garlic oil (0. 4 g/d),
monensin (0. 2 g/d) and turmeric powder (20 g/d). Total apparent
digestibility's (% of intake) of organic matter (OM), dry matter (DM),
crud protein (CP), ether extract(EE), non fiber carbohydrate (NFC),
acid detergent fiber (ADF) and neutral detergent fiber (NDF) in the
total tract were not influenced by addition of either additives.