Utilization of Laser-Ablation Based Analytical Methods for Obtaining Complete Chemical Information of Algae
Themain goal of this article is to find efficient
methods for elemental and molecular analysis of living
microorganisms (algae) under defined environmental conditions and
cultivation processes. The overall knowledge of chemical
composition is obtained utilizing laser-based techniques, Laser-
Induced Breakdown Spectroscopy (LIBS) for acquiring information
about elemental composition and Raman Spectroscopy for gaining
molecular information, respectively. Algal cells were suspended in
liquid media and characterized using their spectra. Results obtained
employing LIBS and Raman Spectroscopy techniques will help to
elucidate algae biology (nutrition dynamics depending on cultivation
conditions) and to identify algal strains, which have the potential for
applications in metal-ion absorption (bioremediation) and biofuel
industry. Moreover, bioremediation can be readily combined with
production of 3rd generation biofuels. In order to use algae for
efficient fuel production, the optimal cultivation parameters have to
be determinedleading to high production of oil in selected
cellswithout significant inhibition of the photosynthetic activity and
the culture growth rate, e.g. it is necessary to distinguish conditions
for algal strain containing high amount of higher unsaturated fatty
acids. Measurements employing LIBS and Raman Spectroscopy were
utilized in order to give information about alga Trachydiscusminutus
with emphasis on the amount of the lipid content inside the algal cell
and the ability of algae to withdraw nutrients from its environment
and bioremediation (elemental composition), respectively. This
article can serve as the reference for further efforts in describing
complete chemical composition of algal samples employing laserablation
techniques.
[1] M. Hannon J. Gimpel, M. Tran, B. Rasala, S. Mayfield, Biofuels from
Algae: challenges and potential, Biofuels 1 (2010) 763-784.
[2] M.F. Demirbas, Biofuel from algae for sustainable development,
Applied Energy 88 (2011) 3437- 3480.
[3] J.B.K. Park, R.J. Craggs, A.N. Shilton, Wastewater treatment high rate
algal ponds for biofuel production, Biosource Technology 102 (2011)
35-42.
[4] A. Demirbas, Use of algae as biofuel sources, Energy Conversion and
Management 51 (2010) 2738- 2749.
[5] Onlinesource.
http://www1.eere.energy.gov/biomass/pdfs/algalbiofuels.pdf, quoted
31.1.2012.
[6] X. Zeng, M.K. Danquah, X.D. Chen, Y. Lu, Microalgae bioengineering:
From CO2 fixation to biofuel production, Renewable and Sustainable
Energy Reviews 15 (2011) 3252-3260.
[7] L. Christenson, R. Sims, Production and harvesting of microalgae for
wastewater treatment, biofuels, and biproducts, Biotechnology
Advances 29 (2011) 686-702.
[8] I. Rawat, R. Ranjith Kumar, T. Mutanda, F. Bux, Dual role of
microalgae: phycoremediation of domestic wastewater and biomass
production for sustainable biofuels production, Applied Energy 88
(2011) 3411-3424.
[9] T.A. Davis, B. Volesky, A. Mucci, A review of the biochemisty of
heavy metal biosorption by brown algae, Water Research 37 (2003)
4311-4330.
[10] M. Hoehse, D. Mory, S. Florek, F. Weritz, I. Gornushkin, U. Panne, A
combined laser-induced breakdown and Raman spectroscopy Echelle
system for elemental and molecular microanalysis.
SpectrochimicaActa Part B 64 (2009) 1219-1227.
[11] M. Sadegh Cheri, S.H. Tavassoli, Quantitative analysis of toxic metals
lead and cadmium in water jet by laser-induced breakdown
spectroscopy, Applied optics 50 (2011) 1227-1233.
[12] P. Porizka, D. Prochazka, J. Novotny, R. Malina, J. Kaiser, O. Samek, L.
Krajcarova, Measurements of algal strain using different LIBS setups.
SpectrochimicaActa 69 (2012) 613-619.
[13] S. Ramya, R.P. George, R.V. SubbaRao, R.K. Dayal, Detection of algae
and bacterial biofilms formed on titanium surfaces using micro-Raman
analysis, Applied Surface Science 256 (2010) 5108-5115.
[14] O. Samek, A. Jon├í┼í, Z. Pil├ít, P. Zem├ínek, L. Nedbal, J. Tř├¡ska, P. Kotas,
M. Trtílek, Raman Microspectroscopy of Individual Algal Cells:
Sensing Unsaturation of Storage Lipids in vivo. Sensors 10 (2010)
8635-8651.
[15] H. Wu, J.V. Volponi, A.E. Oliver, A.N. Parikh, B.A.Simmons, S. Singh,
In vivo lipidomics using single-cell Raman spectroscopy, PNAS 108
(2011) 3809-3814.
[16] T. ┼ÿezanka, M. Petr├ínkov├í. V. Cep├ík, P. Přibyl, K. Sigler, T. Cajthmal,
Trachydiscusminutus, a New Biotechnological Source of
Eicosapentaenoic acid. Folia Microbiol. 55 (3) 265-269.
[17] P. Greenspan, E.P. Mayer, S.D. Fowler, Nile red: A selective fluorescent
stain for intracellular lipid droplets. J. Microbiol. Meth. 68 (2007)
639-642.
[18] B. Ham, R. Shelton, B. Butler, P. Thionville: Calculating the iodine
value for marine oils fatty acid profiles, J. Am. Oil. Chem. Soc. 75
(2008) 4717-4722.
[1] M. Hannon J. Gimpel, M. Tran, B. Rasala, S. Mayfield, Biofuels from
Algae: challenges and potential, Biofuels 1 (2010) 763-784.
[2] M.F. Demirbas, Biofuel from algae for sustainable development,
Applied Energy 88 (2011) 3437- 3480.
[3] J.B.K. Park, R.J. Craggs, A.N. Shilton, Wastewater treatment high rate
algal ponds for biofuel production, Biosource Technology 102 (2011)
35-42.
[4] A. Demirbas, Use of algae as biofuel sources, Energy Conversion and
Management 51 (2010) 2738- 2749.
[5] Onlinesource.
http://www1.eere.energy.gov/biomass/pdfs/algalbiofuels.pdf, quoted
31.1.2012.
[6] X. Zeng, M.K. Danquah, X.D. Chen, Y. Lu, Microalgae bioengineering:
From CO2 fixation to biofuel production, Renewable and Sustainable
Energy Reviews 15 (2011) 3252-3260.
[7] L. Christenson, R. Sims, Production and harvesting of microalgae for
wastewater treatment, biofuels, and biproducts, Biotechnology
Advances 29 (2011) 686-702.
[8] I. Rawat, R. Ranjith Kumar, T. Mutanda, F. Bux, Dual role of
microalgae: phycoremediation of domestic wastewater and biomass
production for sustainable biofuels production, Applied Energy 88
(2011) 3411-3424.
[9] T.A. Davis, B. Volesky, A. Mucci, A review of the biochemisty of
heavy metal biosorption by brown algae, Water Research 37 (2003)
4311-4330.
[10] M. Hoehse, D. Mory, S. Florek, F. Weritz, I. Gornushkin, U. Panne, A
combined laser-induced breakdown and Raman spectroscopy Echelle
system for elemental and molecular microanalysis.
SpectrochimicaActa Part B 64 (2009) 1219-1227.
[11] M. Sadegh Cheri, S.H. Tavassoli, Quantitative analysis of toxic metals
lead and cadmium in water jet by laser-induced breakdown
spectroscopy, Applied optics 50 (2011) 1227-1233.
[12] P. Porizka, D. Prochazka, J. Novotny, R. Malina, J. Kaiser, O. Samek, L.
Krajcarova, Measurements of algal strain using different LIBS setups.
SpectrochimicaActa 69 (2012) 613-619.
[13] S. Ramya, R.P. George, R.V. SubbaRao, R.K. Dayal, Detection of algae
and bacterial biofilms formed on titanium surfaces using micro-Raman
analysis, Applied Surface Science 256 (2010) 5108-5115.
[14] O. Samek, A. Jon├í┼í, Z. Pil├ít, P. Zem├ínek, L. Nedbal, J. Tř├¡ska, P. Kotas,
M. Trtílek, Raman Microspectroscopy of Individual Algal Cells:
Sensing Unsaturation of Storage Lipids in vivo. Sensors 10 (2010)
8635-8651.
[15] H. Wu, J.V. Volponi, A.E. Oliver, A.N. Parikh, B.A.Simmons, S. Singh,
In vivo lipidomics using single-cell Raman spectroscopy, PNAS 108
(2011) 3809-3814.
[16] T. ┼ÿezanka, M. Petr├ínkov├í. V. Cep├ík, P. Přibyl, K. Sigler, T. Cajthmal,
Trachydiscusminutus, a New Biotechnological Source of
Eicosapentaenoic acid. Folia Microbiol. 55 (3) 265-269.
[17] P. Greenspan, E.P. Mayer, S.D. Fowler, Nile red: A selective fluorescent
stain for intracellular lipid droplets. J. Microbiol. Meth. 68 (2007)
639-642.
[18] B. Ham, R. Shelton, B. Butler, P. Thionville: Calculating the iodine
value for marine oils fatty acid profiles, J. Am. Oil. Chem. Soc. 75
(2008) 4717-4722.
@article{"International Journal of Biological, Life and Agricultural Sciences:64968", author = "Pavel Pořízka and David Prochazka and Karel Novotný and Ota Samek and ZdeněkPilát and Klára Procházková and and
Jozef Kaiser", title = "Utilization of Laser-Ablation Based Analytical Methods for Obtaining Complete Chemical Information of Algae", abstract = "Themain goal of this article is to find efficient
methods for elemental and molecular analysis of living
microorganisms (algae) under defined environmental conditions and
cultivation processes. The overall knowledge of chemical
composition is obtained utilizing laser-based techniques, Laser-
Induced Breakdown Spectroscopy (LIBS) for acquiring information
about elemental composition and Raman Spectroscopy for gaining
molecular information, respectively. Algal cells were suspended in
liquid media and characterized using their spectra. Results obtained
employing LIBS and Raman Spectroscopy techniques will help to
elucidate algae biology (nutrition dynamics depending on cultivation
conditions) and to identify algal strains, which have the potential for
applications in metal-ion absorption (bioremediation) and biofuel
industry. Moreover, bioremediation can be readily combined with
production of 3rd generation biofuels. In order to use algae for
efficient fuel production, the optimal cultivation parameters have to
be determinedleading to high production of oil in selected
cellswithout significant inhibition of the photosynthetic activity and
the culture growth rate, e.g. it is necessary to distinguish conditions
for algal strain containing high amount of higher unsaturated fatty
acids. Measurements employing LIBS and Raman Spectroscopy were
utilized in order to give information about alga Trachydiscusminutus
with emphasis on the amount of the lipid content inside the algal cell
and the ability of algae to withdraw nutrients from its environment
and bioremediation (elemental composition), respectively. This
article can serve as the reference for further efforts in describing
complete chemical composition of algal samples employing laserablation
techniques.", keywords = "Laser-Induced Breakdown Spectroscopy, Raman Spectroscopy, Algae, Algal strains, Bioremediation, Biofuels.", volume = "6", number = "12", pages = "1158-5", }