Abstract: As a by-product of the biodiesel industries, glycerol
has been vastly generated which surpasses the market demand. It is
imperative to develop an efficient glycerol valorization processes in
minimizing the net energy requirement and intensifying the biodiesel
production. In this study, base-catalyzed transesterification of
glycerol with dimethyl carbonate using microwave irradiation as
heating method to produce glycerol carbonate was conducted by
varying grades of glycerol, i.e. 70%, 86% and 99% purity, that is
obtained from biodiesel plant. Metal oxide catalysts were used with
varying operating parameters including reaction time, DMC/glycerol
molar ratio, catalyst weight %, temperature and stirring speed. From
the study on the effect of different operating parameters it was found
that the type of catalyst used has the most significant effect on the
transesterification reaction. Amidst the metal oxide catalysts
examined, CaO gave the best performance. This study indicates the
feasibility of producing glycerol carbonate using different grade of
glycerol in both conventional thermal activation and microwave
irradiation with CaO as catalyst. Microwave assisted
transesterification (MAT) of glycerol into glycerol carbonate has
demonstrated itself as an energy efficient route by achieving 94.2%
yield of GC at 65°C, 5 minutes reaction time, 1 wt% CaO and
DMC/glycerol molar ratio of 2. The advantages of MAT
transesterification route has made the direct utilization of bioglycerol
from biodiesel production without the need of purification. This has
marked a more economical and less-energy intensive glycerol
carbonate synthesis route.
Abstract: Anammox is a novel and promising technology that has changed the traditional concept of biological nitrogen removal. The process facilitates direct oxidation of ammonical nitrogen under anaerobic conditions with nitrite as an electron acceptor without addition of external carbon sources. The present study investigated the feasibility of Anammox Hybrid Reactor (AHR) combining the dual advantages of suspended and attached growth media for biodegradation of ammonical nitrogen in wastewater. Experimental unit consisted of 4 nos. of 5L capacity AHR inoculated with mixed seed culture containing anoxic and activated sludge (1:1). The process was established by feeding the reactors with synthetic wastewater containing NH4-H and NO2-N in the ratio 1:1 at HRT (hydraulic retention time) of 1 day. The reactors were gradually acclimated to higher ammonium concentration till it attained pseudo steady state removal at a total nitrogen concentration of 1200 mg/l. During this period, the performance of the AHR was monitored at twelve different HRTs varying from 0.25-3.0 d with increasing NLR from 0.4 to 4.8 kg N/m3d. AHR demonstrated significantly higher nitrogen removal (95.1%) at optimal HRT of 1 day. Filter media in AHR contributed an additional 27.2% ammonium removal in addition to 72% reduction in the sludge washout rate. This may be attributed to the functional mechanism of filter media which acts as a mechanical sieve and reduces the sludge washout rate many folds. This enhances the biomass retention capacity of the reactor by 25%, which is the key parameter for successful operation of high rate bioreactors. The effluent nitrate concentration, which is one of the bottlenecks of anammox process was also minimised significantly (42.3-52.3 mg/L). Process kinetics was evaluated using first order and Grau-second order models. The first-order substrate removal rate constant was found as 13.0 d-1. Model validation revealed that Grau second order model was more precise and predicted effluent nitrogen concentration with least error (1.84±10%). A new mathematical model based on mass balance was developed to predict N2 gas in AHR. The mass balance model derived from total nitrogen dictated significantly higher correlation (R2=0.986) and predicted N2 gas with least error of precision (0.12±8.49%). SEM study of biomass indicated the presence of heterogeneous population of cocci and rod shaped bacteria of average diameter varying from 1.2-1.5 mm. Owing to enhanced NRE coupled with meagre production of effluent nitrate and its ability to retain high biomass, AHR proved to be the most competitive reactor configuration for dealing with nitrogen laden wastewater.
Abstract: Numerous investigations suggest that Mesenchymal
Stem Cells (MSCs) in general represent a valuable tool for therapy of
symptoms related to chronic inflammatory diseases. Blue Horizon
Stem Cell Therapy Program is a leading provider of adult and
children’s stem cell therapies. Uniquely we have safely and
efficiently treated more than 600 patients with documenting each
procedure. The purpose of our study is primarily to monitor the
immune response in order to validate the safety of intravenous
infusion of human umbilical cord blood derived MSCs (UC-MSCs),
and secondly, to evaluate effects on biomarkers associated with
chronic inflammation. Nine patients were treated for conditions
associated with chronic inflammation and for the purpose of antiaging.
They have been given one intravenous infusion of UCMSCs.
Our study of blood test markers of 9 patients with chronic
inflammation before and within three months after MSCs treatment
demonstrates that there is no significant changes and MSCs treatment
was safe for the patients. Analysis of different indicators of chronic
inflammation and aging included in initial, 24-hours, two weeks and
three months protocols showed that stem cell treatment was safe for
the patients; there were no adverse reactions. Moreover data from
follow up protocols demonstrates significant improvement in energy
level, hair, nails growth and skin conditions. Intravenously
administered UC-MSCs were safe and effective in the improvement
of symptoms related to chronic inflammation. Further close
monitoring and inclusion of more patients are necessary to fully
characterize the advantages of UC-MSCs application in treatment of
symptoms related to chronic inflammation.
Abstract: The growing number of computer viruses and the
detection of zero day malware have been the concern for security
researchers for a large period of time. Existing antivirus products
(AVs) rely on detecting virus signatures which do not provide a full
solution to the problems associated with these viruses. The use of
logic formulae to model the behaviour of viruses is one of the most
encouraging recent developments in virus research, which provides
alternatives to classic virus detection methods. In this paper, we
proposed a comparative study about different virus detection
techniques. This paper provides the advantages and drawbacks of
different detection techniques. Different techniques will be used in
this paper to provide a discussion about what technique is more
effective to detect computer viruses.
Abstract: Cadmium (II) (Cd) is one of the major toxic elemental
pollutants, which is hazardous for humans, animals and plants. γ-
Polyglutamic acid (γ-PGA) is an extracellular biopolymer produced
by several species of Bacillus which has been reported to be an
effective biosorbent for metal ions. The effect of γ-PGA on growth of
rice grown under laboratory conditions was investigated. Rice seeds
were germinated and then grown at 30±1°C on filter paper soaked
with Cd solution and γ-PGA for 7 days. The result showed that Cd
significantly inhibited the growth of roots, shoots by reducing root,
and shoot lengths. Fresh and dry weights also decreased compared
with control; however, the addition of 500 mg·L-1 γ-PGA alleviated
rice seedlings from the adverse effects of Cd. The analysis of
physiological traits revealed that Cd caused a decrease in the total
chlorophyll and soluble protein contents and amylase activities in all
treatments. The Cd content in seedling tissues increased for the Cd
250 μM treatment (P
Abstract: In recent years, the use of the aluminum based alloys
in the industry and technology are increasing. Alloying elements in
aluminum have further been improving the strength and stiffness
properties that provide superior compared to other metals. In this
study, investigation of physical properties (microstructure,
microhardness, tensile strength, electrical conductivity and thermal
properties) in the Al-12.6wt.%Si-%2wt.Ni ternary alloy were
investigated. Al-Si-Ni alloy was prepared in vacuum atmosphere. The
samples were directionally solidified upwards with different growth
rate V (8.3−165.45 μm/s) at constant temperature gradient G (7.73
K/mm). The flake spacings (λ), microhardness (HV), ultimate tensile
strength (σ), electrical resistivity (ρ) and thermal properties (H, Cp,
Tm) of the samples were measured. Influence of the growth rate and
spacings on microhardness, ultimate tensile strength and electrical
resistivity were investigated and relationships between them were
obtained. According to results, λ values decrease with increasing V,
but HV, σ and ρ values increase with increasing V. Variations of
electrical resistivity (ρ) of solidified samples were also measured.
The enthalpy of fusion (H) and specific heat (Cp) for the alloy was
also determined by differential scanning calorimeter (DSC) from
heating trace during the transformation from liquid to solid. The
results in this work were compared with the previous similar
experimental results.
Abstract: The paper presents an advanced control system for
tennis ball throwing machines to improve their accuracy according to
the ball impact points. A further advantage of the system is the much
easier calibration process involving the intelligent solution of the
automatic adjustment of the stroking parameters according to the ball
elasticity, the self-calibration, the use of the safety margin at very flat
strokes and the possibility to placing the machine to any position of
the half court. The system applies mathematical methods to
determine the exact ball trajectories and special approximating
processes to access all points on the aimed half court.
Abstract: This paper treats different aspects of entropy measure
in classical information theory and statistical quantum mechanics, it
presents the possibility of extending the definition of Von Neumann
entropy to image and array processing. In the first part, we generalize
the quantum entropy using singular values of arbitrary rectangular
matrices to measure the randomness and the quality of denoising
operation, this new definition of entropy can be implemented to
compare the performance analysis of filtering methods. In the second
part, we apply the concept of pure state in quantum formalism
to generalize the maximum entropy method for narrowband and
farfield source localization problem. Several computer simulation
results are illustrated to demonstrate the effectiveness of the proposed
techniques.
Abstract: Batch production plants provide a wide range of
scheduling problems. In pharmaceutical industries a batch process
is usually described by a recipe, consisting of an ordering of tasks
to produce the desired product. In this research work we focused
on pharmaceutical production processes requiring the culture of
a microorganism population (i.e. bacteria, yeasts or antibiotics).
Several sources of uncertainty may influence the yield of the culture
processes, including (i) low performance and quality of the cultured
microorganism population or (ii) microbial contamination. For
these reasons, robustness is a valuable property for the considered
application context. In particular, a robust schedule will not collapse
immediately when a cell of microorganisms has to be thrown away
due to a microbial contamination. Indeed, a robust schedule should
change locally in small proportions and the overall performance
measure (i.e. makespan, lateness) should change a little if at all.
In this research work we formulated a constraint programming
optimization (COP) model for the robust planning of antibiotics
production. We developed a discrete-time model with a multi-criteria
objective, ordering the different criteria and performing a
lexicographic optimization. A feasible solution of the proposed
COP model is a schedule of a given set of tasks onto available
resources. The schedule has to satisfy tasks precedence constraints,
resource capacity constraints and time constraints. In particular
time constraints model tasks duedates and resource availability
time windows constraints. To improve the schedule robustness, we
modeled the concept of (a, b) super-solutions, where (a, b) are input
parameters of the COP model. An (a, b) super-solution is one in
which if a variables (i.e. the completion times of a culture tasks)
lose their values (i.e. cultures are contaminated), the solution can be
repaired by assigning these variables values with a new values (i.e.
the completion times of a backup culture tasks) and at most b other
variables (i.e. delaying the completion of at most b other tasks).
The efficiency and applicability of the proposed model is
demonstrated by solving instances taken from a real-life
pharmaceutical company. Computational results showed that
the determined super-solutions are near-optimal.
Abstract: The global demand for long-tailed macaques for
medical experimentation has continued to increase. Fulfillment of
Indonesian export demands has been mostly from natural habitats,
based on a harvesting quota. This quota has been determined
according to the total catch for a given year, and not based on
consideration of any demographic parameters or physical
environmental factors with regard to the animal; hence threatening
the sustainability of the various populations. It is therefore necessary
to formulate a method for calculating a sustainable harvesting quota,
based on population parameters in natural habitats. Considering the
possibility of variations in habitat characteristics and population
parameters, a time series observation of demographic and
physical/biotic parameters, in various habitats, was performed on 13
groups of long-tailed macaques, distributed throughout the West
Java, Lampung and Yogyakarta areas of Indonesia. These provinces
were selected for comparison of the influence of human/tourism
activities. Data on population parameters that was collected included
data on life expectancy according to age class, numbers of
individuals by sex and age class, and ‘ratio of infants to reproductive
females’. The estimation of population growth was based on a
population dynamic growth model: the Leslie matrix. The harvesting
quota was calculated as being the difference between the actual
population size and the MVP (minimum viable population) for each
sex and age class. Observation indicated that there were variations within group size
(24–106 individuals), gender (sex) ratio (1:1 to 1:1.3), life expectancy
value (0.30 to 0.93), and ‘ratio of infants to reproductive females’
(0.23 to 1.56). Results of subsequent calculations showed that
sustainable harvesting quotas for each studied group of long-tailed
macaques, ranged from 29 to 110 individuals. An estimation model
of the MVP for each age class was formulated as Log Y = 0.315 +
0.884 Log Ni (number of individual on ith age class). This study also
found that life expectancy for the juvenile age class was affected by
the humidity under tree stands, and dietary plants’ density at sapling,
pole and tree stages (equation: Y=2.296 – 1.535 RH + 0.002 Kpcg –
0.002 Ktg – 0.001 Kphn, R2 = 89.6% with a significance value of
0.001). By contrast, for the sub-adult-adult age class, life expectancy
was significantly affected by slope (equation: Y=0.377 = 0.012 Kml,
R2 = 50.4%, with significance level of 0.007). The infant-toreproductive-
female ratio was affected by humidity under tree stands,
and dietary plant density at sapling and pole stages (equation: Y = -
1.432 + 2.172 RH – 0.004 Kpcg + 0.003 Ktg, R2 = 82.0% with
significance level of 0.001). This research confirmed the importance
of population parameters in determining the minimum viable
population, and that MVP varied according to habitat characteristics
(especially food availability). It would be difficult therefore, to
formulate a general mathematical equation model for determining a
harvesting quota for the species as a whole.
Abstract: Barley (Hordeum vulgare L.), vetch (Vicia villosa),
and grass pea (Lathyrus sativus L.) monocultures as well as mixtures
of barley with each of the above legumes, in three seeding ratios (i.e.,
barley: legume 75:25, 50:50 and 25:75, based on seed numbers) were
used to investigated forage yield and competition indices. The results
showed that intercropping reduced the dry matter yield of the three
component plants, compared with their respective monocrops. The
greatest value of total dry matter yield was obtained from barley25-
grasspea75 (5.44 t ha-1) mixture, followed by grass pea sole crop (4.99
t ha-1). The total actual yield loss (AYL) values were positive and
greater than 0 in all mixtures, indicating an advantage from
intercropping over sole crops. Intercropped barley had a higher
relative crowding coefficient (K=1.64) than intercropped legumes
(K=1.20), indicating that barley was more competitive than legumes
in mixtures. Furthermore, grass pea was more competitive than vetch
in mixtures with barley. The highest land equivalent ratio (LER),
system productivity index (SPI) and monetary advantage index
(MAI) were obtained when barley was mixed at a rate of 25% with
75% seed rate of grass pea. It is concluded that intercropping of
barley with grass pea has a good potential to improve the
performance of forage with high land-use efficiency.
Abstract: This paper discusses the propagation of sound waves in
air, specifically in narrow rectangular pathways of an occluded-ear
simulator for acoustic measurements. In narrow pathways, both the
speed of sound and the phase of the sound waves are affected by the
damping of the air viscosity. Herein, we propose a new finite-element
method (FEM) that considers the effects of the air viscosity. The
method was developed as an extension of existing FEMs for porous,
sound-absorbing materials. The results of a numerical calculation for a
three-dimensional ear-simulator model using the proposed FEM were
validated by comparing with theoretical lumped-parameter modeling
analysis and standard values.
Abstract: An approach was evaluated for the retrieval of soil
moisture of bare soil surface using bistatic scatterometer data in the
angular range of 200 to 700 at VV- and HH- polarization. The
microwave data was acquired by specially designed X-band (10
GHz) bistatic scatterometer. The linear regression analysis was done
between scattering coefficients and soil moisture content to select the
suitable incidence angle for retrieval of soil moisture content. The 250
incidence angle was found more suitable. The support vector
regression analysis was used to approximate the function described
by the input output relationship between the scattering coefficient and
corresponding measured values of the soil moisture content. The
performance of support vector regression algorithm was evaluated by
comparing the observed and the estimated soil moisture content by
statistical performance indices %Bias, root mean squared error
(RMSE) and Nash-Sutcliffe Efficiency (NSE). The values of %Bias,
root mean squared error (RMSE) and Nash-Sutcliffe Efficiency
(NSE) were found 2.9451, 1.0986 and 0.9214 respectively at HHpolarization.
At VV- polarization, the values of %Bias, root mean
squared error (RMSE) and Nash-Sutcliffe Efficiency (NSE) were
found 3.6186, 0.9373 and 0.9428 respectively.
Abstract: In this research, a questionnaire survey was conducted
to measure nap, drowsiness and fatigue of drivers who work for long
shifts, to discuss about the work environment and health conditions for
taxi and bus drivers who work at night-time. The questionnaire sheet
used for this research was organized into the following categories:
tension/tiredness, drowsiness while driving, and the nap situation
during night-time work. The number of taxi drivers was 127 and the
number of bus drivers was 40. Concerning the results of a comparison
of nap hours of taxi and bus drivers, the taxi drivers’ nap hours are
overwhelmingly shorter, and also the frequency of drivers who
experience drowsiness is higher. The burden on bus drivers does not
change because of the system of a two-driver rotation shift. In
particular, the working environment of the taxi driver may lead to
greater fatigue accumulation than the bus driver’s environment.
Abstract: Effects of nicotine on pre-partum body weight and
preimplantation embryonic development has been reported
previously. Present study was conducted to determine the effects of
annatto (Bixa orellana)-derived delta-tocotrienol (TCT) (with
presence of 10% gamma-TCT isomer) on the nicotine-induced
reduction in body weight and 8-cell embryonic growth in mice.
Twenty-four 6-8 weeks old (23-25g) female balb/c mice were
randomly divided into four groups (G1-G4; n=6). Those groups were
subjected to the following treatments for 7 consecutive days: G1
(control) were gavaged with 0.1 ml tocopherol stripped corn oil. G2
was subcutaneously (s.c.) injected with 3 mg/kg/day of nicotine. G3
received concurrent treatment of nicotine (3 mg/kg/day) and 60
mg/kg/day of δ-TCT mixture (contains 90% delta & 10% gamma
isomers) and G4 was given 60 mg/kg/day of δ-TCT mixture alone.
Body weights were recorded daily during the treatment. On Day 8,
females were superovulated with 5 IU Pregnant Mare’s Serum
Gonadotropin (PMSG) for 48 hours followed with 5 IU human
Chorionic Gonadotropin (hCG) before mated with males at the ratio
of 1:1. Females were sacrificed by cervical dislocation for embryo
collection 48 hours post-coitum. Collected embryos were cultured in
vitro. Results showed that throughout Day 1 to Day 7, the body
weight of nicotine treated group (G2) was significantly lower
(p
Abstract: Studying on the response of vegetation phenology to
climate change at different temporal and spatial scales is important for
understanding and predicting future terrestrial ecosystem dynamics
and the adaptation of ecosystems to global change. In this study, the
Moderate Resolution Imaging Spectroradiometer (MODIS)
Normalized Difference Vegetation Index (NDVI) dataset and climate
data were used to analyze the dynamics of grassland phenology as well
as their correlation with climatic factors in different eco-geographic
regions and elevation units across the Tibetan Plateau. The results
showed that during 2003–2012, the start of the grassland greening
season (SOS) appeared later while the end of the growing season
(EOS) appeared earlier following the plateau’s precipitation and heat
gradients from southeast to northwest. The multi-year mean value of
SOS showed differences between various eco-geographic regions and
was significantly impacted by average elevation and regional average
precipitation during spring. Regional mean differences for EOS were
mainly regulated by mean temperature during autumn. Changes in
trends of SOS in the central and eastern eco-geographic regions were
coupled to the mean temperature during spring, advancing by about
7d/°C. However, in the two southwestern eco-geographic regions,
SOS was delayed significantly due to the impact of spring
precipitation. The results also showed that the SOS occurred later with
increasing elevation, as expected, with a delay rate of 0.66 d/100m.
For 2003–2012, SOS showed an advancing trend in low-elevation
areas, but a delayed trend in high-elevation areas, while EOS was
delayed in low-elevation areas, but advanced in high-elevation areas.
Grassland SOS and EOS changes may be influenced by a variety of
other environmental factors in each eco-geographic region.
Abstract: The development of transport systems has negative
impacts on the environment although it has beneficial effects on
society. The car policy caused many problems such as: - the
spectacular growth of fuel consumption hence the very vast increase
in urban pollution, traffic congestion in certain places and at certain
times, the increase in the number of accidents. The exhaust emissions
from cars and weather conditions are the main factors that determine
the level of pollution in urban atmosphere. These conditions lead to
the phenomenon of heat transfer and radiation occurring between the
air and the soil surface of any town. These exchanges give rise, in
urban areas, to the effects of heat islands that correspond to the
appearance of excess air temperature between the city and its
surrounding space. In this object, we perform a numerical simulation
of the plume generated by the cars exhaust gases and show that these
gases form a screening effect above the urban city which cause the
heat island in the presence of wind flow. This study allows us: 1. To
understand the different mechanisms of interactions between these
phenomena.2. To consider appropriate technical solutions to mitigate
the effects of the heat island.
Abstract: Public space is essential to strengthen the social and
urban fabric and the social cohesion; there lies the importance of its
study. Hence, the aim of this paper is to analyze the quality of public
space in the XXI century in both quantitative and qualitative terms. In
this article, the concept of public space includes open spaces such as
parks, public squares and walking areas. To make this analysis, we
take Mexico City as the case study. It has a population of nearly 9
million inhabitants and is composed of sixteen boroughs. For this
analysis, we consider both existing public spaces and the government
intervention for building and improving new and existent public
spaces. Results show that on the one hand, quantitatively there is not
an equitable distribution of public spaces due to both the growth of
the city itself as well as due to the absence of political will to create
public spaces. Another factor is the evolution of this city, which has
been growing merely in a “patched pattern”, where public space has
played no role at all with a total absence of urban design. On the
other hand, qualitatively, even the boroughs with the most public
spaces have not shown interest in making these spaces qualitatively
inclusive and open to the general population aiming for integration.
Therefore, urban projects that privatize public space seem to be the
rule, rather than a rehabilitation effort of the existent public spaces.
Hence, state intervention should reinforce its role as an agent of
social change acting in benefit of the majority of the inhabitants with
the promotion of more inclusive public spaces.
Abstract: This study analyzes the critical gaps in the
architecture of European stability and the expected role of the
banking union as the new important step towards completing the
Economic and Monetary Union that should enable the creation of
safe and sound financial sector for the euro area market. The single
rulebook together with the Single Supervisory Mechanism and the
Single Resolution Mechanism - as two main pillars of the banking
union, should provide a consistent application of common rules and
administrative standards for supervision, recovery and resolution of
banks – with the final aim of replacing the former bail-out practice
with the bail-in system through which possible future bank failures
would be resolved by their own funds, i.e. with minimal costs for
taxpayers and real economy. In this way, the vicious circle between
banks and sovereigns would be broken. It would also reduce the
financial fragmentation recorded in the years of crisis as the result of
divergent behaviors in risk premium, lending activities and interest
rates between the core and the periphery. In addition, it should
strengthen the effectiveness of monetary transmission channels, in
particular the credit channels and overflows of liquidity on the money
market which, due to the fragmentation of the common financial
market, has been significantly disabled in period of crisis. However,
contrary to all the positive expectations related to the future
functioning of the banking union, major findings of this study
indicate that characteristics of the economic system in which the
banking union will operate should not be ignored. The euro area is an
integration of strong and weak entities with large differences in
economic development, wealth, assets of banking systems, growth
rates and accountability of fiscal policy. The analysis indicates that
low and unbalanced economic growth remains a challenge for the
maintenance of financial stability and this problem cannot be
resolved just by a single supervision. In many countries bank assets
exceed their GDP by several times and large banks are still a matter
of concern, because of their systemic importance for individual
countries and the euro zone as a whole. The creation of the Single
Supervisory Mechanism and the Single Resolution Mechanism is a
response to the European crisis, which has particularly affected
peripheral countries and caused the associated loop between the
banking crisis and the sovereign debt crisis, but has also influenced
banks’ balance sheets in the core countries, as the result of crossborder
capital flows. The creation of the SSM and the SRM should
prevent the similar episodes to happen again and should also provide
a new opportunity for strengthening of economic and financial
systems of the peripheral countries. On the other hand, there is a
potential threat that future focus of the ECB, resolution mechanism
and other relevant institutions will be extremely oriented towards
large and significant banks (whereby one half of them operate in the
core and most important euro area countries), and therefore it remains
questionable to what extent will the common resolution funds will be used for rescue of less important institutions. Recent geopolitical
developments will be the optimal indicator to show whether the
previously established mechanisms are sufficient enough to maintain
the adequate financial stability in the euro area market.
Abstract: Tannase (tannin acyl hydrolase, E.C.3.1.1.20) is an
important hydrolysable enzyme with innumerable applications and
industrial potential. In the present study, a kinetic model has been
developed for the batch fermentation used for the production of
tannase by A.flavus MTCC 3783. Maximum tannase activity of
143.30 U/ml was obtained at 96 hours under optimum operating
conditions at 35oC, an initial pH of 5.5 and with an inducer tannic
acid concentration of 3% (w/v) for a fermentation period of 120
hours. The biomass concentration reaches a maximum of 6.62 g/l at
96 hours and further there was no increase in biomass concentration
till the end of the fermentation. Various unstructured kinetic models
were analyzed to simulate the experimental values of microbial
growth, tannase activity and substrate concentration. The Logistic
model for microbial growth , Luedeking - Piret model for production
of tannase and Substrate utilization kinetic model for utilization of
substrate were capable of predicting the fermentation profile with
high coefficient of determination (R2) values of 0.980, 0.942 and
0.983 respectively. The results indicated that the unstructured models
were able to describe the fermentation kinetics more effectively.