Abstract: Cytogenetic analysis still remains the gold standard method for prenatal diagnosis of trisomy 21 (Down syndrome, DS). Nevertheless, the conventional cytogenetic analysis needs live cultured cells and is too time-consuming for clinical application. In contrast, molecular methods such as FISH, QF-PCR, MLPA and quantitative Real-time PCR are rapid assays with results available in 24h. In the present study, we have successfully used a novel MGB TaqMan probe-based real time PCR assay for rapid diagnosis of trisomy 21 status in Down syndrome samples. We have also compared the results of this molecular method with corresponding results obtained by the cytogenetic analysis. Blood samples obtained from DS patients (n=25) and normal controls (n=20) were tested by quantitative Real-time PCR in parallel to standard G-banding analysis. Genomic DNA was extracted from peripheral blood lymphocytes. A high precision TaqMan probe quantitative Real-time PCR assay was developed to determine the gene dosage of DSCAM (target gene on 21q22.2) relative to PMP22 (reference gene on 17p11.2). The DSCAM/PMP22 ratio was calculated according to the formula; ratio=2 -ΔΔCT. The quantitative Real-time PCR was able to distinguish between trisomy 21 samples and normal controls with the gene ratios of 1.49±0.13 and 1.03±0.04 respectively (p value
Abstract: This paper presents a novel method for inferring the
odor based on neural activities observed from rats- main olfactory
bulbs. Multi-channel extra-cellular single unit recordings were done
by micro-wire electrodes (tungsten, 50μm, 32 channels) implanted in
the mitral/tufted cell layers of the main olfactory bulb of anesthetized
rats to obtain neural responses to various odors. Neural response
as a key feature was measured by substraction of neural firing rate
before stimulus from after. For odor inference, we have developed a
decoding method based on the maximum likelihood (ML) estimation.
The results have shown that the average decoding accuracy is about
100.0%, 96.0%, 84.0%, and 100.0% with four rats, respectively. This
work has profound implications for a novel brain-machine interface
system for odor inference.
Abstract: Freeze concentration freezes or crystallises the water
molecules out as ice crystals and leaves behind a highly concentrated
solution. In conventional suspension freeze concentration where ice
crystals formed as a suspension in the mother liquor, separation of
ice is difficult. The size of the ice crystals is still very limited which
will require usage of scraped surface heat exchangers, which is very
expensive and accounted for approximately 30% of the capital cost.
This research is conducted using a newer method of freeze
concentration, which is progressive freeze concentration. Ice crystals
were formed as a layer on the designed heat exchanger surface. In
this particular research, a helical structured copper crystallisation
chamber was designed and fabricated. The effect of two operating
conditions on the performance of the newly designed crystallisation
chamber was investigated, which are circulation flowrate and coolant
temperature. The performance of the design was evaluated by the
effective partition constant, K, calculated from the volume and
concentration of the solid and liquid phase. The system was also
monitored by a data acquisition tool in order to see the temperature
profile throughout the process. On completing the experimental
work, it was found that higher flowrate resulted in a lower K, which
translated into high efficiency. The efficiency is the highest at 1000
ml/min. It was also found that the process gives the highest
efficiency at a coolant temperature of -6 °C.
Abstract: Gastric ulceration is a discontinuity in gastric mucosa, usually occurs due to imbalance between the gastric mucosal protective factors, that is called gastric mucosal barrier, and the aggressive factors, to which the mucosa is exposed. This study was carried out on sixty male Sprague-Dowely rats (12- 16 weeks old) allocated into two groups. The first control group and the second Gastric lesion group which induced by oral administration of a single daily dose of aspirin at a dose of 300 mg/kg body weight for 7 consecutive-days (6% aspirin solution will be prepared and each rat will be given 5 ml of that solution/kg body weight). Blood is collected 1, 2 and 3 weeks after induction of gastric ulceration. Significant increase in serum copper, nitric oxide, and prostaglandin E2 all over the period of experiment. Significant decrease in erythrocyte superoxide dismutase (t-SOD) activities, serum (calcium, phosphorus, glucose and insulin) levels. Non-significant changes in serum sodium and potassium levels are obtained.
Abstract: Mixed convection in two-dimensional shallow rectangular enclosure is considered. The top hot wall moves with constant velocity while the cold bottom wall has no motion. Simulations are performed for Richardson number ranging from Ri = 0.001 to 100 and for Reynolds number keeping fixed at Re = 408.21. Under these conditions cavity encompasses three regimes: dominating forced, mixed and free convection flow. The Prandtl number is set to 6 and the effects of cavity inclination on the flow and heat transfer are studied for different Richardson number. With increasing the inclination angle, interesting behavior of the flow and thermal fields are observed. The streamlines and isotherm plots and the variation of the Nusselt numbers on the hot wall are presented. The average Nusselt number is found to increase with cavity inclination for Ri ³ 1 . Also it is shown that the average Nusselt number changes mildly with the cavity inclination in the dominant forced convection regime but it increases considerably in the regime with dominant natural convection.
Abstract: In this work, a new approach is proposed to control
the manipulators for Humanoid robot. The kinematics of the
manipulators in terms of joint positions, velocity, acceleration and
torque of each joint is computed using the Denavit Hardenberg (D-H)
notations. These variables are used to design the manipulator control
system, which has been proposed in this work. In view of supporting
the development of a controller, a simulation of the manipulator is
designed for Humanoid robot. This simulation is developed through
the use of the Virtual Reality Toolbox and Simulink in Matlab. The
Virtual Reality Toolbox in Matlab provides the interfacing and
controls to an environment which is developed based on the Virtual
Reality Modeling Language (VRML). Chains of bones were used to
represent the robot.
Abstract: Anti-money laundering is commonly recognized as a
set of procedures, laws or regulations designed to reduce the practice
of generating income through illegal actions. In Malaysia, the
government and law enforcement agencies have stepped up their
capacities and efforts to curb money laundering since 2001. One of
these measures was the enactment of the Anti-Money Laundering
Act (AMLA) in 2001. The implementation costs on anti-money
laundering requirements (AMLR) can be burdensome to those who
are involved in enforcing them. The objective of this paper is to
explore the perceived effectiveness of AMLR from the enforcement
agencies- perspective. This is a preliminary study whose findings
will help to give direction for further AML research in Malaysia. In
addition, the results of this study provide empirical evidences on the
perceived effectiveness of AMLR prior to further investigations on
barriers and improvements of the implementation of the anti-money
laundering regime in Malaysia.
Abstract: Flash memory has become an important storage device
in many embedded systems because of its high performance, low
power consumption and shock resistance. Multi-level cell (MLC) is
developed as an effective solution for reducing the cost and increasing
the storage density in recent years. However, most of flash file system
cannot handle the error correction sufficiently. To correct more errors
for MLC, we implement Reed-Solomon (RS) code to YAFFS, what is
widely used for flash-based file system. RS code has longer computing
time but the correcting ability is much higher than that of Hamming
code.
Abstract: Adapting wireless devices to communicate within grid
networks empowers us by providing range of possibilities.. These
devices create a mechanism for consumers and publishers to create
modern networks with or without peer device utilization. Emerging
mobile networks creates new challenges in the areas of reliability,
security, and adaptability. In this paper, we propose a system
encompassing mobility management using AAA context transfer for
mobile grid networks. This system ultimately results in seamless task
processing and reduced packet loss, communication delays,
bandwidth, and errors.
Abstract: Wireless Sensor Network is Multi hop Self-configuring
Wireless Network consisting of sensor nodes. The deployment of
wireless sensor networks in many application areas, e.g., aggregation
services, requires self-organization of the network nodes into clusters.
Efficient way to enhance the lifetime of the system is to partition the
network into distinct clusters with a high energy node as cluster head.
The different methods of node clustering techniques have appeared in
the literature, and roughly fall into two families; those based on the
construction of a dominating set and those which are based solely on
energy considerations. Energy optimized cluster formation for a set
of randomly scattered wireless sensors is presented. Sensors within a
cluster are expected to be communicating with cluster head only. The
energy constraint and limited computing resources of the sensor nodes
present the major challenges in gathering the data. In this paper we
propose a framework to study how partially correlated data affect the
performance of clustering algorithms. The total energy consumption
and network lifetime can be analyzed by combining random geometry
techniques and rate distortion theory. We also present the relation
between compression distortion and data correlation.
Abstract: The alternative technique for sterilization of culture
medium to replace autoclaving was carried out. For sterilization of
culture medium without autoclaving, some commercial pure essential
oils, bergamot oil, betel oil, cinnamon oil, lavender oil and turmeric
oil, were tested alone or in combinations with some disinfectants,
10% povidone-iodine and 2% iodine + 2.4% potassium iodide. Each
essential oil or combination was added to 25-mL Murashige and
Skoog (MS) medium before medium was solidified in a 120-mL
container, kept for 2 weeks before evaluating sterile conditions.
Treated media, supplemented with essential oils, were compared to
control medium, autoclaved at 121 degree Celsius for 15 min. In
vitro sterile conditions were found 20 – 100% from these treated
media compared to 100% sterile condition from autoclaved medium.
Treated media obtained 100% sterile conditions were chosen for
culturing chrysanthemum shoots. It was found that 10% povidoneiodine
in combination with cinnamon oil (3:1) and 2% iodine + 2.4%
potassium iodide in combination with lavender oil (1:3) at the
concentration of 36 3L/25 mL medium provided the promising
growth of shoot explants.
Abstract: This study examined the toxicological effects and
safety of polypeptide k isolated from the seeds of Momordica
charantia in laboratory rats. 30 male Sprague Dawley rats (12 weeks
old, bodyweight 180-200 g) were randomly divided into 3 groups
(1000 mg/kg, 500 mg and 0 mg/kg). Rats were acclimatized to
laboratory conditions for 7 days and at day 8 rats were dosed orally
with polypeptide k (in 2% DMSO/normal saline) and the controls
received the dosed vehicle only. Rats were then observed for 72
hours before sacrificed. Rats were anaesthetized by pentobarbital
(50 mg/kg ip) and 2-3.0 mL of blood was taken by cardiac puncture
and rats were scarified by anaesthetic overdose. Immediately, organs
(heart, lungs, liver, kidneys) were weigh and taken for histology.
Organ sections were then evaluated by a histopathologist. Serum
samples were assayed for liver functions (ALT and γ-GT) and kidney
functions (BUN and creatinine). All rats showed normal behavior
after the dosing and no statistical changes were observed in all blood
parameters and organ weight. Histological examinations revealed
normal organ structures. In conclusion, dosing of rats up to 1000
mg/kg did not have any effects on the rat behavior, liver or kidney
functions nor histology of the selected organs.
Abstract: In a 10-week (May – August, 2008) Phase I trial, 840, 1+ rainbow trout, Oncorhynchus mykiss, received a commercial oral immunomodulator, Fin Immune™, at four different dosages (0, 10, 20 and 30 mg g-1) to evaluate immune response and growth. The overall objective of was to determine an optimal dosage of this product for rainbow trout that provides enhanced immunity with maximal growth and health. Biweekly blood samples were taken from 10 randomly selected fish in each tank (30 samples per treatment) to evaluate the duration of enhanced immunity conferred by Fin-Immune™. The immunological assessment included serum white blood cell (lymphocyte, neutrophil) densities and blood hematocrit (packed cell volume %). Of these three variables, only lymphocyte density increased significantly among trout fed Fin- Immune™ at 20 and 30 mg g-1 which peaked at week 6. At week 7, all trout were switched to regular feed (lacking Fin-Immune™) and by week 10, lymphocyte levels decreased among all levels but were still greater than at week 0. There was growth impairment at the highest dose of Fin-Immune™ tested (30 mg g-1) which can be associated with a physiological compensatory mechanism due to a dose-specific threshold level. Thus, our main objective of this Phase I study was achieved, the 20 mg g-1 dose of Fin-Immune™ should be the most efficacious (of those we tested) to use for a Phase II disease challenge trial.
Abstract: We describe an effective method for image encryption
which employs magnitude and phase manipulation using carrier
images. Although it involves traditional methods like magnitude and
phase encryptions, the novelty of this work lies in deploying the
concept of carrier images for encryption purpose. To this end, a
carrier image is randomly chosen from a set of stored images. One
dimensional (1-D) discrete Fourier transform (DFT) is then carried
out on the original image to be encrypted along with the carrier
image. Row wise spectral addition and scaling is performed between
the magnitude spectra of the original and carrier images by randomly
selecting the rows. Similarly, row wise phase addition and scaling is
performed between the original and carrier images phase spectra by
randomly selecting the rows. The encrypted image obtained by these
two operations is further subjected to one more level of magnitude
and phase manipulation using another randomly chosen carrier image
by 1-D DFT along the columns. The resulting encrypted image is
found to be fully distorted, resulting in increasing the robustness
of the proposed work. Further, applying the reverse process at the
receiver, the decrypted image is found to be distortionless.
Abstract: The purpose of the present work was to study the
production and process parameters optimization for the synthesis of
cellulase from Trichoderma viride in solid state fermentation (SSF)
using an agricultural wheat straw as substrates; as fungal conversion
of lignocellulosic biomass for cellulase production is one among the
major increasing demand for various biotechnological applications.
An optimization of process parameters is a necessary step to get
higher yield of product. Several kinetic parameters like pretreatment,
extraction solvent, substrate concentration, initial moisture content,
pH, incubation temperature and inoculum size were optimized for
enhanced production of third most demanded industrially important
cellulase. The maximum cellulase enzyme activity 398.10±2.43
μM/mL/min was achieved when proximally analyzed lignocellulosic
substrate wheat straw inocubated at 2% HCl as pretreatment tool
along with distilled water as extraction solvent, 3% substrate
concentration 40% moisture content with optimum pH 5.5 at 45°C
incubation temperature and 10% inoculum size.
Abstract: In this paper, free vibration analysis of carbon nanotube (CNT) reinforced laminated composite panels is presented. Three types of panels such as flat, concave and convex are considered for study. Numerical simulation is carried out using commercially available finite element analysis software ANSYS. Numerical homogenization is employed to calculate the effective elastic properties of randomly distributed carbon nanotube reinforced composites. To verify the accuracy of the finite element method, comparisons are made with existing results available in the literature for conventional laminated composite panels and good agreements are obtained. The results of the CNT reinforced composite materials are compared with conventional composite materials under different boundary conditions.
Abstract: The problem of lot sizing, sequencing and scheduling
multiple products in flow line production systems has been studied
by several authors. Almost all of the researches in this area assumed
that setup times and costs are sequence –independent even though
sequence dependent setups are common in practice. In this paper we
present a new mixed integer non linear program (MINLP) and a
heuristic method to solve the problem in sequence dependent case.
Furthermore, a genetic algorithm has been developed which applies
this constructive heuristic to generate initial population. These two
proposed solution methods are compared on randomly generated
problems. Computational results show a clear superiority of our
proposed GA for majority of the test problems.
Abstract: The growing health hazardous impact of arsenic (As)
contamination in environment is the impetus of the present
investigation. Application of lactic acid bacteria (LAB) for the
removal of toxic and heavy metals from water has been reported.
This study was performed in order to isolate and characterize the Asresistant
LAB from mud and sludge samples for using as efficient As
uptaking probiotic. Isolation of As-resistant LAB colonies was
performed by spread plate technique using bromocresol purple
impregnated-MRS (BP-MRS) agar media provided with As @ 50
μg/ml. Isolated LAB were employed for probiotic characterization
process, acid and bile tolerance, lactic acid production, antibacterial
activity and antibiotic tolerance assays. After As-resistant and
removal characterizations, the LAB were identified using 16S rDNA
sequencing. A total of 103 isolates were identified as As-resistant
strains of LAB. The survival of 6 strains (As99-1, As100-2, As101-3,
As102-4, As105-7, and As112-9) was found after passing through the
sequential probiotic characterizations. Resistant pattern pronounced
hollow zones at As concentration >2000 μg/ml in As99-1, As100-2,
and As101-3 LAB strains, whereas it was found at ~1000 μg/ml in
rest 3 strains. Among 6 strains, the As uptake efficiency of As102-4
(0.006 μg/h/mg wet weight of cell) was higher (17 – 209%)
compared to remaining LAB. 16S rDNA sequencing data of 3 (As99-
1, As100-2, and As101-3) and 3 (As102-4, As105-7, and As112-9)
LAB strains clearly showed 97 to 99% (340 bp) homology to
Pediococcus dextrinicus and Pediococcus acidilactici, respectively.
Though, there was no correlation between the metal resistant and
removal efficiency of LAB examined but identified elevated As
removing LAB would probably be a potential As uptaking probiotic
agent. Since present experiment concerned with only As removal
from pure water, As removal and removal mechanism in natural
condition of intestinal milieu should be assessed in future studies.
Abstract: XML is a markup language which is becoming the
standard format for information representation and data exchange. A
major purpose of XML is the explicit representation of the logical
structure of a document. Much research has been performed to
exploit logical structure of documents in information retrieval in
order to precisely extract user information need from large
collections of XML documents. In this paper, we describe an XML
information retrieval weighting scheme that tries to find the most
relevant elements in XML documents in response to a user query.
We present this weighting model for information retrieval systems
that utilize plausible inferences to infer the relevance of elements in
XML documents. We also add to this model the Dempster-Shafer
theory of evidence to express the uncertainty in plausible inferences
and Dempster-Shafer rule of combination to combine evidences
derived from different inferences.
Abstract: Aquatic and semi aquatic birds as a group are suited to
feed and breed in environments in which water forms a fundamental
part. These birds are biological indicator in aquatic environment,
because these birds belong to the top level of food chain in aquatic
ecosystems. There are 61 species in 14 families of aquatic and semi
aquatic birds in Iran. The birds of the Sattarkhan Lake belong to 16
species in 8 families which include 26.2 percent of total Aquatic and
semi aquatic bird species and 57% of Aquatic and semi aquatic bird's
family of Iran. Study was carried out monthly at Sattarkhan Lake
show the existence of Phalacrocorax carbo, Ardea cinerea, Egretta
alba, Egretta garzetta, Bubulcus ibis, Botaurus stellaris, Sterna
hirundo, Chlidonias leucopterus, Larus minutus, Larus argentatus,
Larus ridibunbus, Alcedo atthis, Ciconia ciconia, Plegadis
falcinellus, Circus aeruginosus, Corvus frugilegus