Abstract: Ethanol is generally used as a therapeutic reagent against Hepatocellular carcinoma (HCC or hepatoma) worldwide, as it can induce Hepatocellular carcinoma cell apoptosis at low concentration through a multifactorial process regulated by several unknown proteins. This paper provides a simple and available proteomic strategy for exploring differentially expressed proteins in the apoptotic pathway. The appropriate concentrations of ethanol required to induce HepG2 cell apoptosis were first assessed by MTT assay, Gisma and fluorescence staining. Next, the central proteins involved in the apoptosis pathway processs were determined using 2D-PAGE, SDS-PAGE, and bio-software analysis. Finally the downregulation of two proteins, AFP and survivin, were determined by immunocytochemistry and reverse transcriptase PCR (RT-PCR) technology. The simple, useful method demonstrated here provides a new approach to proteomic analysis in key bio-regulating process including proliferation, differentiation, apoptosis, immunity and metastasis.
Abstract: The P-Bigram method is a string comparison methods
base on an internal two characters-based similarity measure. The edit
distance between two strings is the minimal number of elementary
editing operations required to transform one string into the other. The
elementary editing operations include deletion, insertion, substitution
two characters. In this paper, we address the P-Bigram method to
sole the similarity problem in DNA sequence. This method provided
an efficient algorithm that locates all minimum operation in a string.
We have been implemented algorithm and found that our program
calculated that smaller distance than one string. We develop PBigram
edit distance and show that edit distance or the similarity and
implementation using dynamic programming. The performance of
the proposed approach is evaluated using number edit and percentage
similarity measures.
Abstract: This work considered the thermodynamic feasibility
of scrubbing volatile organic compounds into biodiesel in view of
designing a gas treatment process with this absorbent. A detailed
vapour – liquid equilibrium investigation was performed using the
original UNIFAC group contribution method. The four biodiesels
studied in this work are methyl oleate, methyl palmitate, methyl
linolenate and ethyl stearate. The original UNIFAC procedure was
used to estimate the infinite dilution activity coefficients of 13
selected volatile organic compounds in the biodiesels. The
calculations were done at the VOC mole fraction of 9.213x10-8. Ethyl
stearate gave the most favourable phase equilibrium. A close
agreement was found between the infinite dilution activity coefficient
of toluene found in this work and those reported in literature.
Thermodynamic models can efficiently be used to calculate vast
amount of phase equilibrium behaviour using limited number of
experimental data.
Abstract: It has been shown that the solution of water shortage problem in Central Asia closely connected with inclusion of atmosphere water vapour into the system of response and water resources management. Some methods of water extraction from atmosphere have been discussed.
Abstract: This paper presents the simulation of fragmentation
warhead using a hydrocode, Autodyn. The goal of this research is to
determine the lethal range of such a warhead. This study investigates
the lethal range of warheads with and without steel balls as
preformed fragments. The results from the FE simulation, i.e. initial
velocities and ejected spray angles of fragments, are further processed
using an analytical approach so as to determine a fragment hit density
and probability of kill of a modelled warhead. In order to simulate a
plenty of preformed fragments inside a warhead, the model requires
expensive computation resources. Therefore, this study attempts to
model the problem in an alternative approach by considering an
equivalent mass of preformed fragments to the mass of warhead
casing. This approach yields approximately 7% and 20% difference
of fragment velocities from the analytical results for one and two
layers of preformed fragments, respectively. The lethal ranges of the
simulated warheads are 42.6 m and 56.5 m for warheads with one and
two layers of preformed fragments, respectively, compared to 13.85
m for a warhead without preformed fragment. These lethal ranges are
based on the requirement of fragment hit density. The lethal ranges
which are based on the probability of kill are 27.5 m, 61 m and 70 m
for warheads with no preformed fragment, one and two layers of
preformed fragments, respectively.
Abstract: In this paper dynamics of a vapour bubble generated
due to a local energy input inside a vertical rigid cylinder and in the
absence of buoyancy forces is investigated. Different ratios of the
diameter of the rigid cylinder to the maximum radius of the bubble
are considered. The Boundary Integral Equation Method is employed
for numerical simulation of the problem. Results show that during
the collapse phase of the bubble inside a vertical rigid cylinder, two
liquid micro jets are developed on the top and bottom sides of the
vapour bubble and are directed inward. Results also show that
existence of a deposit rib inside the vertical rigid cylinder slightly
increases the life time of the bubble. It is found that by increasing the
ratio of the cylinder diameter to the maximum radius of the bubble,
the rate of the growth and collapse phases of the bubble increases
and the life time of the bubble decreases.
Abstract: The fluorescent pseudomonad strain R81 is a root
colonizing rhizobacteria which promotes the growth of many plants
by various mechanisms. Its broth containing siderophore (ironchelating
compound) and 2,4- diacetyl phloroglucinol (DAPG) is
used for preparing bioinoculant formulations for agronomical
applications. Glycerol was found to be the best carbon source for
improved biomass production. Splitting of nitrogen source to NH4Cl
and urea had a stabilizing effect on pH during batch cultivation. Ltryptophan
at 0.5 % in the medium increased the siderophore
production to 850 mg/l. During batch cultivation of the strain in a
bioreactor, a maximum of 4 g/l of dry cell mass, 1.8 g/l of
siderophore and 20 mg/l of DAPG was achieved when glycerol was
15 g/l and C/N ratio was maintained at 12.5. In case of intermittent
feeding of fresh medium during fed-batch cultivation, the dry cell
mass was increased to 25 g/l with improved production of DAPG to
70 mg/l.
Abstract: Monoculture and mixed cultures of microalgae and
the oleaginous yeast for microbial oil productions were investigated
using sugarcane juice as carbon substrate. The monoculture of yeast
Torulaspora maleeae Y30, Torulaspora globosa YU5/2 grew faster
than that of microalgae Chlorella sp. KKU-S2. In monoculture of T.
maleeae Y30, a biomass of 8.267g/L with lipid yield of 0.920g/L
were obtained, while 8.333g/L of biomass with lipid yield of
1.141g/L were obtained for monoculture of T. globosa YU5/2. A
biomass of 1.933g/L with lipid yield of 0.052g/L was found for
monoculture of Chlorella sp. KKU-S2. The biomass concentration in
the mixed culture of the oleaginous yeast with microalgae increased
faster and was higher compared with that in the monocultures. A
biomass of 8.733g/L with lipid yield of 1.564g/L was obtained for a
mixed culture of T. maleeae Y30 with Chlorella sp. KKU-S2, while
8.010g/L of biomass with lipid yield of 2.424g/L was found for
mixed culture of T. globosa YU5/2 with Chlorella sp. KKU-S2.
Maximum cell yield coefficient (YX/S, g/L) was found of 0.323 in
monoculture of Chlorella sp. KKU-S2 but low level of both specific
yield of lipid (YP/X, g lipid/g cells) of 0.027 and volumetric lipid
production rate (QP, g/L/d) of 0.003 were observed. While, maximum
YP/X (0.303), QP (0.105) and maximum process product yield (YP/S,
0.061) were obtained in mixed culture of T. globosa YU5/2 with
Chlorella sp. KKU-S2. The results obtained from the study shows
that mixed culture of yeast with microalgae is a desirable cultivation
process for microbial oil production.
Abstract: If an unsteady heat transfer or heat impulse happens in
part of the cryogenic pipeline system of large space environment
simulation equipment while running in vacuum environment, it will
lead to abnormal flow of the cryogenic fluid in the pipeline. When the
situation gets worse, the cryogenic fluid in the pipeline will have phase
change and a gas block which results in the malfunction of the
cryogenic pipeline system. Referring to the structural parameter of a
typical cryogenic pipeline system and the basic equation, an analytical
model and a calculation model for cryogenic pipeline system can be
built. The various factors which influence the thermal resistance of a
cryogenic pipeline system can be analyzed and calculated by using the
qualitative analysis relation deduced for thermal resistance of pipeline.
The research conclusion could provide theoretical support for the
design and operation of a cryogenic pipeline system
Abstract: ZnO-SnO2 i.e. Zinc-Tin-Oxide (ZTO) thin films were
deposited on glass substrate with varying concentrations (ZnO:SnO2
- 100:0, 90:10, 70:30 and 50:50 wt.%) at room temperature by flash
evaporation technique. These deposited ZTO film were annealed at
450 0C in vacuum. These films were characterized to study the effect
of annealing on the structural, electrical, and optical properties.
Atomic force microscopy (AFM) and Scanning electron microscopy
(SEM) images manifest the surface morphology of these ZTO thin
films. The apparent growth of surface features revealed the formation
of nanostructure ZTO thin films. The small value of surface
roughness (root mean square RRMS) ensures the usefulness in
optical coatings. The sheet resistance was also found to be decreased
for both types of films with increasing concentration of SnO2. The
optical transmittance found to be decreased however blue shift has
been observed after annealing.
Abstract: This paper describes a steady state model of a multiple
effect evaporator system for simulation and control purposes. The
model includes overall as well as component mass balance equations,
energy balance equations and heat transfer rate equations for area
calculations for all the effects. Each effect in the process is
represented by a number of variables which are related by the energy
and material balance equations for the feed, product and vapor flow
for backward, mixed and split feed. For simulation 'fsolve' solver in
MATLAB source code is used. The optimality of three sequences i.e.
backward, mixed and splitting feed is studied by varying the various
input parameters.
Abstract: The mixture formation prior to the ignition process
plays as a key element in the diesel combustion. Parametric studies of
mixture formation and ignition process in various injection parameter
has received considerable attention in potential for reducing
emissions. Purpose of this study is to clarify the effects of injection
pressure on mixture formation and ignition especially during ignition
delay period, which have to be significantly influences throughout the
combustion process and exhaust emissions. This study investigated
the effects of injection pressure on diesel combustion fundamentally
using rapid compression machine. The detail behavior of mixture
formation during ignition delay period was investigated using the
schlieren photography system with a high speed camera. This method
can capture spray evaporation, spray interference, mixture formation
and flame development clearly with real images. Ignition process and
flame development were investigated by direct photography method
using a light sensitive high-speed color digital video camera. The
injection pressure and air motion are important variable that strongly
affect to the fuel evaporation, endothermic and prolysis process
during ignition delay. An increased injection pressure makes spray tip
penetration longer and promotes a greater amount of fuel-air mixing
occurs during ignition delay. A greater quantity of fuel prepared
during ignition delay period thus predominantly promotes more rapid
heat release.
Abstract: In this paper, the solubility of CO2 in AMP solution
have been measured at temperature range of ( 293, 303 ,313,323)
K.The amine concentration ranges studied are (2.0, 2.8, and 3.4) M.
A solubility apparatus was used to measure the solubility of CO2 in
AMP solution on samples of flue gases from Thermal and Central
Power Plants of Esfahan Steel Company. The modified Kent
Eisenberg model was used to correlate and predict the vapor-liquid
equilibria of the (CO2 + AMP + H2O) system. The model predicted
results are in good agreement with the experimental vapor-liquid
equilibrium measurements.
Abstract: There are two types of drought as conceptual drought
and operational drought. The three parameters as the beginning, the
end and the degree of severity of the drought can be identifying in
operational drought by average precipitation in the whole region. One
of the methods classified to measure drought is Reconnaissance
Drought Index (RDI). Evapotranspiration is calculated using
Penman-Monteith method by analyzing thirty nine years prolong
climatic data. The evapotranspiration is then utilized in RDI to
classify normalized and standardized RDI. These RDI classifications
led to what kind of drought faced in Bhavnagar region on 12 month
time scale basis. The comparison between actual drought conditions
and RDI method used to find out drought are also illustrated. It can
be concluded that the index results of drought in a particular year are
same in both methods but having different index values where as
severity remain same.
Abstract: Observations show that power plant efficiency
decreases in hot summer days. Water droplet injection in air
condensers is suggested in order to decrease the inlet air temperature.
Nozzle arrangement, injected water flow rate and droplets diameter
effects on evaporation rate and the resulting air temperature are
investigated using numerical simulation. Decreasing the diameter of
injected droplets and increasing the number of injecting nozzles,
decreases the outlet air temperature. Also a more uniform air
temperature can be obtained using more injecting nozzles. Numerical
results are in good agreement with analytical results.
Abstract: This research paper presents numerical studies of the
characteristics of warhead fragmentation in terms of initial velocities,
spray angles of fragments and fragment mass distribution of high
explosive (HE) warhead. The behavior of warhead fragmentation
depends on shape and size of warhead, thickness of casing, type of
explosive, number and position of detonator, and etc. This paper
focuses on the effects of material properties of warhead casing, i.e.
failure strain, initial yield and ultimate strength on the characteristics
of warhead fragmentation. It was found that initial yield and ultimate
strength of casing has minimal effects on the initial velocities and
spray angles of fragments. Moreover, a brittle warhead casing with
low failure strain tends to produce higher number of fragments with
less average fragment mass.
Abstract: Compared to oil production from microorganisms, little work has been performed for mixed culture of microalgae and yeast. In this article it is aimed to show high oil accumulation potential of mixed culture of microalgae Chlorella sp. KKU-S2 and oleaginous yeast Torulaspora maleeae Y30 using sugarcane molasses as substrate. The monoculture of T. maleeae Y30 grew faster than that of microalgae Chlorella sp. KKU-S2. In monoculture of yeast, a biomass of 6.4g/L with specific growth rate (m) of 0.265 (1/d) and lipid yield of 0.466g/L were obtained, while 2.53g/L of biomass with m of 0.133 (1/d) and lipid yield of 0.132g/L were obtained for monoculture of Chlorella sp. KKU-S2. The biomass concentration in the mixed culture of T. maleeae Y30 with Chlorella sp. KKU-S2 increased faster and was higher compared with that in the monoculture and mixed culture of microalgae. In mixed culture of microalgae Chlorella sp. KKU-S2 and C. vulgaris TISTR8580, a biomass of 3.47g/L and lipid yield of 0.123 g/L were obtained. In mixed culture of T. maleeae Y30 with Chlorella sp. KKU-S2, a maximum biomass of 7.33 g/L and lipid yield of 0.808g/L were obtained. Maximum cell yield coefficient (YX/S, 0.229g/L), specific yield of lipid (YP/X, 0.11g lipid/g cells) and volumetric lipid production rate (QP, 0.115 g/L/d) were obtained in mixed culture of yeast and microalgae. Clearly, T. maleeae Y30 and Chlorella sp. KKU-S2 use sugarcane molasses as organic nutrients efficiently in mixed culture under mixotrophic growth. The biomass productivity and lipid yield are notably enhanced in comparison with monoculture.
Abstract: The intention of this study to design the probability optimized sewing sack-s workstation based on ergonomics for productivity improvement and decreasing musculoskeletal disorders. The physical dimensions of two workers were using to design the new workstation. The physical dimensions are (1) sitting height, (2) mid shoulder height sitting, (3) shoulder breadth, (4) knee height, (5) popliteal height, (6) hip breadth and (7) buttock-knee length. The 5th percentile of buttock knee length sitting (51 cm), the 50th percentile of mid shoulder height sitting (62 cm) and the 95th percentile of popliteal height (43 cm) and hip breadth (45 cm) applied to design the workstation for sewing sack-s operator and the others used to adjust the components of this workstation. The risk assessment by RULA before and after using the probability optimized workstation were 7 and 7 scores and REBA scores were 11 and 5, respectively. Body discomfort-abnormal index was used to assess muscle fatigue of operators before adjustment workstation found that neck muscles, arm muscles area, muscles on the back and the lower back muscles fatigue. Therefore, the extension and flexion exercise was applied to relief musculoskeletal stresses. The workers exercised 15 minutes before the beginning and the end of work for 5 days. After that, the capability of flexion and extension muscles- workers were increasing in 3 muscles (arm, leg, and back muscles).
Abstract: Bone marrow-derived stem cells have been widely
studied as an alternative source of stem cells. Mesenchymal stem
cells (MSCs) were mostly investigated and studies showed MSCs can
promote neurogenesis. Little is known about the non-mesenchymal
mononuclear cell fraction, which contains both hematopoietic and
nonhematopoietic cells, including monocytes and endothelial
progenitor cells. This study focused on unfractionated bone marrow
mononuclear cells (BMMCs), which remained 72 h after MSCs were
adhered to the culture plates. We showed that BMMC-conditioned
medium promoted morphological changes of human SH-SY5Y
neuroblastoma cells from an epithelial-like phenotype towards a
neuron-like phenotype as indicated by an increase in neurite
outgrowth, like those observed in retinoic acid (RA)-treated cells.
The result could be explained by the effects of trophic factors
released from BMMCs, as shown in the RT-PCR results that
BMMCs expressed nerve growth factor (NGF), brain-derived
neurotrophic factor (BDNF), and ciliary neurotrophic factor (CNTF).
Similar results on the cell proliferation rate were also observed
between RA-treated cells and cells cultured in BMMC-conditioned
medium, suggesting that cells creased proliferating and differentiated
into a neuronal phenotype. Using real-time RT-PCR, a significantly
increased expression of tyrosine hydroxylase (TH) mRNA in SHSY5Y
cells indicated that BMMC-conditioned medium induced
catecholaminergic identities in differentiated SH-SY5Y cells.
Abstract: This paper tests the level of market integration between Malaysia and Singapore stock markets with the world market. Kalman Filter (KF) methodology is used on the International Capital Asset Pricing Model (ICAPM) and the pricing errors estimated within the framework of ICAPM are used as a measure of market integration or segmentation. The advantage of the KF technique is that it allows for time-varying coefficients in estimating ICAPM and hence able to capture the varying degree of market integration. Empirical results show clear evidence of varying degree of market integration for both case of Malaysia and Singapore. Furthermore, the results show that the changes in the level of market integration are found to coincide with certain economic events that have taken placed. The findings certainly provide evidence on the practicability of the KF technique to estimate stock markets integration. In the comparison between Malaysia and Singapore stock market, the result shows that the trends of the market integration indices for Malaysia and Singapore look similar through time but the magnitude is notably different with the Malaysia stock market showing greater degree of market integration. Finally, significant evidence of varying degree of market integration shows the inappropriate use of OLS in estimating the level of market integration.