Abstract: D-erythro-cyclohexylserine (D
chiral unnatural β-hydroxy amino acid expected for the synthesis of drug for AIDS treatment. To develop a continuous bioconversion
system with whole cell biocatalyst of D-threonine aldolase (D genes for the D-erythro-CHS production, D-threonine aldolase gene
was amplified from Ensifer arboris 100383 by direct PCR amplication using two degenerated oligonucleotide primers designed based on
genomic sequence of Shinorhizobium meliloti
Sequence analysis of the cloned DNA fragment revealed one
open-reading frame of 1059 bp and 386 amino acids. This putative
D-TA gene was cloned into NdeI and EcoRI (pEnsi
His-tag sequence or BamHI (pEnsi-DTA[2])
sequence of the pET21(a) vector. The expression level of the cloned gene was extremely overexpressed by E. coli BL21(DE3) transformed with pEnsi-DTA[1] compared to E. coli BL21(DE3) transformed with
pEnsi-DTA[2]. When the cells expressing the wild
used for D-TA enzyme activity, 12 mM glycine was successfully
detected in HPLC analysis. Moreover, the whole cells harbouring the
recombinant D-TA was able to synthesize D-erythro
of 0.6 mg/ml in a batch reaction.
Abstract: European Union candidate status provides a
strong motivation for decision-making in the candidate
countries in shaping the regional development policy where
there is an envisioned transfer of power from center to the
periphery. The process of Europeanization anticipates the
candidate countries configure their regional institutional
templates in the context of the requirements of the European
Union policies and introduces new instruments of incentive
framework of enlargement to be employed in regional
development schemes. It is observed that the contribution of
the local actors to the decision making in the design of the
allocation architectures enhances the efficiency of the funds
and increases the positive effects of the projects funded under
the regional development objectives. This study aims at
exploring the performances of the three regional development
grant schemes in Turkey, established and allocated under the
pre-accession process with a special emphasis given to the
roles of the national and local actors in decision-making for
regional development. Efficiency analyses have been
conducted using the DEA methodology which has proved to
be a superior method in comparative efficiency and
benchmarking measurements. The findings of this study as
parallel to similar international studies, provides that the
participation of the local actors to the decision-making in
funding contributes both to the quality and the efficiency of
the projects funded under the EU schemes.
Abstract: Due to the recovering global economy, enterprises are
increasingly focusing on logistics. Investing in logistic measures for
a production generates a large potential for achieving a good starting
point within a competitive field. Unlike during the global economic
crisis, enterprises are now challenged with investing available capital
to maximize profits. In order to be able to create an informed and
quantifiably comprehensible basis for a decision, enterprises need an
adequate model for logistically and monetarily evaluating measures
in production. The Collaborate Research Centre 489 (SFB 489) at the
Institute for Production Systems (IFA) developed a Logistic
Information System which provides support in making decisions and
is designed specifically for the forging industry. The aim of a project
that has been applied for is to now transfer this process in order to
develop a universal approach to logistically and monetarily evaluate
measures in production.
Abstract: This paper describes a new supervised fusion (hybrid)
electrocardiogram (ECG) classification solution consisting of a new
QRS complex geometrical feature extraction as well as a new version
of the learning vector quantization (LVQ) classification algorithm
aimed for overcoming the stability-plasticity dilemma. Toward this
objective, after detection and delineation of the major events of ECG
signal via an appropriate algorithm, each QRS region and also its
corresponding discrete wavelet transform (DWT) are supposed as
virtual images and each of them is divided into eight polar sectors.
Then, the curve length of each excerpted segment is calculated
and is used as the element of the feature space. To increase the
robustness of the proposed classification algorithm versus noise,
artifacts and arrhythmic outliers, a fusion structure consisting of
five different classifiers namely as Support Vector Machine (SVM),
Modified Learning Vector Quantization (MLVQ) and three Multi
Layer Perceptron-Back Propagation (MLP–BP) neural networks with
different topologies were designed and implemented. The new proposed
algorithm was applied to all 48 MIT–BIH Arrhythmia Database
records (within–record analysis) and the discrimination power of the
classifier in isolation of different beat types of each record was
assessed and as the result, the average accuracy value Acc=98.51%
was obtained. Also, the proposed method was applied to 6 number
of arrhythmias (Normal, LBBB, RBBB, PVC, APB, PB) belonging
to 20 different records of the aforementioned database (between–
record analysis) and the average value of Acc=95.6% was achieved.
To evaluate performance quality of the new proposed hybrid learning
machine, the obtained results were compared with similar peer–
reviewed studies in this area.
Abstract: Information is power. Geographical information is an
emerging science that is advancing the development of knowledge to
further help in the understanding of the relationship of “place" with
other disciplines such as crime. The researchers used crime data for
the years 2004 to 2007 from the Baguio City Police Office to
determine the incidence and actual locations of crime hotspots.
Combined qualitative and quantitative research methodology was
employed through extensive fieldwork and observation, geographic
visualization with Geographic Information Systems (GIS) and Global
Positioning Systems (GPS), and data mining. The paper discusses
emerging geographic visualization and data mining tools and
methodologies that can be used to generate baseline data for
environmental initiatives such as urban renewal and rejuvenation.
The study was able to demonstrate that crime hotspots can be
computed and were seen to be occurring to some select places in the
Central Business District (CBD) of Baguio City. It was observed that
some characteristics of the hotspot places- physical design and milieu
may play an important role in creating opportunities for crime. A list
of these environmental attributes was generated. This derived
information may be used to guide the design or redesign of the urban
environment of the City to be able to reduce crime and at the same
time improve it physically.
Abstract: As networking has become popular, Web-learning
tends to be a trend while designing a tool. Moreover, five-axis
machining has been widely used in industry recently; however, it has
potential axial table colliding problems. Thus this paper aims at
proposing an efficient web-learning collision detection tool on
five-axis machining. However, collision detection consumes heavy
resource that few devices can support, thus this research uses a
systematic approach based on web knowledge to detect collision. The
methodologies include the kinematics analyses for five-axis motions,
separating axis method for collision detection, and computer
simulation for verification. The machine structure is modeled as STL
format in CAD software. The input to the detection system is the
g-code part program, which describes the tool motions to produce the
part surface. This research produced a simulation program with C
programming language and demonstrated a five-axis machining
example with collision detection on web site. The system simulates the
five-axis CNC motion for tool trajectory and detects for any collisions
according to the input g-codes and also supports high-performance
web service benefiting from C. The result shows that our method
improves 4.5 time of computational efficiency, comparing to the
conventional detection method.
Abstract: This research contribution is drafted to present the
orbit design, orbit propagator and geomagnetic field estimator for the
nanosatellites specifically for the upcoming CUBESAT, ICUBE-1 of
the Institute of Space Technology (IST), Islamabad, Pakistan. The
ICUBE mission is designed for the low earth orbit at the approximate
height of 700KM. The presented research endeavor designs the
Keplarian elements for ICUBE-1 orbit while incorporating the
mission requirements and propagates the orbit using J2 perturbations,
The attitude determination system of the ICUBE-1 consists of
attitude determination sensors like magnetometer and sun sensor. The
Geomagnetic field estimator is developed according to the model of
International Geomagnetic Reference Field (IGRF) for comparing the
magnetic field measurements by the magnetometer for attitude
determination. The output of the propagator namely the Keplarians
position and velocity vectors and the magnetic field vectors are
compared and verified with the same scenario generated in the
Satellite Tool Kit (STK).
Abstract: The paper presents an overview of environmental
issues that may be expected with nuclear desalination. The analysis
of coupling nuclear power with desalination plants indicates that
adverse marine impacts can be mitigated with alternative intake
designs or cooling systems. The atmospheric impact of desalination
may be greatly reduced through the coupling with nuclear power,
while maximizing the socio-economic benefit for both processes. The
potential for tritium contamination of the desalinated water was
reviewed. Experience with the systems and practices related to the
radiological quality of the product water, shows no examples of
cross-contamination. Furthermore, the indicators for the public
acceptance of nuclear desalination, as one of the most important
sustainability aspects of any such large project, show a positive trend.
From the data collected, a conclusion is made that nuclear
desalination should be supported by decision-makers.
Abstract: In this paper is investigated a possible
optimization of some linear algebra problems which can be
solved by parallel processing using the special arrays called
systolic arrays. In this paper are used some special types of
transformations for the designing of these arrays. We show
the characteristics of these arrays. The main focus is on
discussing the advantages of these arrays in parallel
computation of matrix product, with special approach to the
designing of systolic array for matrix multiplication.
Multiplication of large matrices requires a lot of
computational time and its complexity is O(n3 ). There are
developed many algorithms (both sequential and parallel) with
the purpose of minimizing the time of calculations. Systolic
arrays are good suited for this purpose. In this paper we show
that using an appropriate transformation implicates in finding
more optimal arrays for doing the calculations of this type.
Abstract: The main goal of the present work is to decrease the
computational burden for optimum design of steel frames with
frequency constraints using a new type of neural networks called
Wavelet Neural Network. It is contested to train a suitable neural
network for frequency approximation work as the analysis program.
The combination of wavelet theory and Neural Networks (NN)
has lead to the development of wavelet neural networks.
Wavelet neural networks are feed-forward networks using
wavelet as activation function. Wavelets are mathematical
functions within suitable inner parameters, which help them to
approximate arbitrary functions. WNN was used to predict the
frequency of the structures. In WNN a RAtional function with
Second order Poles (RASP) wavelet was used as a transfer
function. It is shown that the convergence speed was faster
than other neural networks. Also comparisons of WNN with
the embedded Artificial Neural Network (ANN) and with
approximate techniques and also with analytical solutions are
available in the literature.
Abstract: Phishing, or stealing of sensitive information on the
web, has dealt a major blow to Internet Security in recent times. Most
of the existing anti-phishing solutions fail to handle the fuzziness
involved in phish detection, thus leading to a large number of false
positives. This fuzziness is attributed to the use of highly flexible and
at the same time, highly ambiguous HTML language. We introduce a
new perspective against phishing, that tries to systematically prove,
whether a given page is phished or not, using the corresponding
original page as the basis of the comparison. It analyzes the layout of
the pages under consideration to determine the percentage distortion
between them, indicative of any form of malicious alteration. The
system design represents an intelligent system, employing dynamic
assessment which accurately identifies brand new phishing attacks
and will prove effective in reducing the number of false positives.
This framework could potentially be used as a knowledge base, in
educating the internet users against phishing.
Abstract: A new multi inner stage (MIS) cyclone was designed to
remove the acidic gas and fine particles produced from electronic
industry. To characterize gas flow in MIS cyclone, pressure and
velocity distribution were calculated by means of CFD program. Also,
the flow locus of fine particles and particle removal efficiency were
analyzed by Lagrangian method. When outlet pressure condition was
–100mmAq, the efficiency was the best in this study.
Abstract: Emergence of smartphones brings to live the concept
of converged devices with the availability of web amenities. Such
trend also challenges the mobile devices manufactures and service
providers in many aspects, such as security on mobile phones,
complex and long time design flow, as well as higher development
cost. Among these aspects, security on mobile phones is getting more
and more attention. Microkernel based virtualization technology will
play a critical role in addressing these challenges and meeting mobile
market needs and preferences, since virtualization provides essential
isolation for security reasons and it allows multiple operating systems
to run on one processor accelerating development and cutting development
cost. However, virtualization benefits do not come for free.
As an additional software layer, it adds some inevitable virtualization
overhead to the system, which may decrease the system performance.
In this paper we evaluate and analyze the virtualization performance
cost of L4 microkernel based virtualization on a competitive mobile
phone by comparing the L4Linux, a para-virtualized Linux on top of
L4 microkernel, with the native Linux performance using lmbench
and a set of typical mobile phone applications.
Abstract: Recent trends in building constructions in Libya are
more toward tall (high-rise) building projects. As a consequence, a
better estimation of the lateral loading in the design process is
becoming the focal of a safe and cost effective building industry. Byin-
large, Libya is not considered a potential earthquake prone zone,
making wind is the dominant design lateral loads. Current design
practice in the country estimates wind speeds on a mere random
bases by considering certain factor of safety to the chosen wind
speed. Therefore, a need for a more accurate estimation of wind
speeds in Libya was the motivation behind this study. Records of
wind speed data were collected from 22 metrological stations in
Libya, and were statistically analysed. The analysis of more than four
decades of wind speed records suggests that the country can be
divided into four zones of distinct wind speeds. A computer “survey"
program was manipulated to draw design wind speeds contour map
for the state of Libya.
The paper presents the statistical analysis of Libya-s recorded
wind speed data and proposes design wind speed values for a 50-year
return period that covers the entire country.
Abstract: Most routing protocols (DSR, AODV etc.) that have
been designed for wireless adhoc networks incorporate the broadcasting
operation in their route discovery scheme. Probabilistic broadcasting
techniques have been developed to optimize the broadcast operation
which is otherwise very expensive in terms of the redundancy
and the traffic it generates. In this paper we have explored percolation
theory to gain a different perspective on probabilistic broadcasting
schemes which have been actively researched in the recent years.
This theory has helped us estimate the value of broadcast probability
in a wireless adhoc network as a function of the size of the network.
We also show that, operating at those optimal values of broadcast
probability there is at least 25-30% reduction in packet regeneration
during successful broadcasting.
Abstract: Complex engineering design problems consist of
numerous factors of varying criticalities. Considering fundamental features of design and inferior details alike will result in an extensive
waste of time and effort. Design parameters should be introduced gradually as appropriate based on their significance relevant to the
problem context. This motivates the representation of design parameters at multiple levels of an abstraction hierarchy. However, developing abstraction hierarchies is an area that is not well
understood. Our research proposes a novel hierarchical abstraction methodology to plan effective engineering designs and processes. It
provides a theoretically sound foundation to represent, abstract and stratify engineering design parameters and tasks according to causality and criticality. The methodology creates abstraction
hierarchies in a recursive and bottom-up approach that guarantees no
backtracking across any of the abstraction levels. The methodology consists of three main phases, representation, abstraction, and layering to multiple hierarchical levels. The effectiveness of the
developed methodology is demonstrated by a design problem.
Abstract: Corporate credit rating prediction using statistical and
artificial intelligence (AI) techniques has been one of the attractive
research topics in the literature. In recent years, multiclass
classification models such as artificial neural network (ANN) or
multiclass support vector machine (MSVM) have become a very
appealing machine learning approaches due to their good
performance. However, most of them have only focused on classifying
samples into nominal categories, thus the unique characteristic of the
credit rating - ordinality - has been seldom considered in their
approaches. This study proposes new types of ANN and MSVM
classifiers, which are named OMANN and OMSVM respectively.
OMANN and OMSVM are designed to extend binary ANN or SVM
classifiers by applying ordinal pairwise partitioning (OPP) strategy.
These models can handle ordinal multiple classes efficiently and
effectively. To validate the usefulness of these two models, we applied
them to the real-world bond rating case. We compared the results of
our models to those of conventional approaches. The experimental
results showed that our proposed models improve classification
accuracy in comparison to typical multiclass classification techniques
with the reduced computation resource.
Abstract: Speedups from mapping four real-life DSP
applications on an embedded system-on-chip that couples coarsegrained
reconfigurable logic with an instruction-set processor are
presented. The reconfigurable logic is realized by a 2-Dimensional
Array of Processing Elements. A design flow for improving
application-s performance is proposed. Critical software parts, called
kernels, are accelerated on the Coarse-Grained Reconfigurable
Array. The kernels are detected by profiling the source code. For
mapping the detected kernels on the reconfigurable logic a prioritybased
mapping algorithm has been developed. Two 4x4 array
architectures, which differ in their interconnection structure among
the Processing Elements, are considered. The experiments for eight
different instances of a generic system show that important overall
application speedups have been reported for the four applications.
The performance improvements range from 1.86 to 3.67, with an
average value of 2.53, compared with an all-software execution.
These speedups are quite close to the maximum theoretical speedups
imposed by Amdahl-s law.
Abstract: The study of human hand morphology reveals that developing an artificial hand with the capabilities of human hand is an extremely challenging task. This paper presents the development of a robotic prosthetic hand focusing on the improvement of a tendon driven mechanism towards a biomimetic prosthetic hand. The design of this prosthesis hand is geared towards achieving high level of dexterity and anthropomorphism by means of a new hybrid mechanism that integrates a miniature motor driven actuation mechanism, a Shape Memory Alloy actuated mechanism and a passive mechanical linkage. The synergy of these actuators enables the flexion-extension movement at each of the finger joints within a limited size, shape and weight constraints. Tactile sensors are integrated on the finger tips and the finger phalanges area. This prosthesis hand is developed with an exact size ratio that mimics a biological hand. Its behavior resembles the human counterpart in terms of working envelope, speed and torque, and thus resembles both the key physical features and the grasping functionality of an adult hand.
Abstract: Facility Layout Problem (FLP) is one of the essential
problems of several types of manufacturing and service sector. It is
an optimization problem on which the main objective is to obtain the
efficient locations, arrangement and order of the facilities. In the
literature, there are numerous facility layout problem research
presented and have used meta-heuristic approaches to achieve
optimal facility layout design. This paper presented genetic algorithm
to solve facility layout problem; to minimize total cost function. The
performance of the proposed approach was verified and compared
using problems in the literature.