Abstract: The balance between nitrogen loading and runoff in the
forested headwater streams of the Kanna River was estimated to
elucidate the current status of nitrogen saturation in a forested
watershed. NO3-N concentration in the study area was far higher than
the average value in Japan. Estimated nitrogen runoff accounted for
55–57% of nitrogen loading; suggesting that the forest-s nitrogen
retention capacity is most likely in decline. Since the 1970s, Japan-s
forestry industry has been declining due to the decrease in lumber
demand and increase in cheap imported materials. Thus, this decline
will contribute significantly to further reducing nitrogen saturation in
forest ecosystems.
Abstract: With the hardware technology advancing, the cost of
storing is decreasing. Thus there is an urgent need for new techniques
and tools that can intelligently and automatically assist us in
transferring this data into useful knowledge. Different techniques of
data mining are developed which are helpful for handling these large
size databases [7]. Data mining is also finding its role in the field of
biotechnology. Pedigree means the associated ancestry of a crop
variety. Genetic diversity is the variation in the genetic composition
of individuals within or among species. Genetic diversity depends
upon the pedigree information of the varieties. Parents at lower
hierarchic levels have more weightage for predicting genetic
diversity as compared to the upper hierarchic levels. The weightage
decreases as the level increases. For crossbreeding, the two varieties
should be more and more genetically diverse so as to incorporate the
useful characters of the two varieties in the newly developed variety.
This paper discusses the searching and analyzing of different possible
pairs of varieties selected on the basis of morphological characters,
Climatic conditions and Nutrients so as to obtain the most optimal
pair that can produce the required crossbreed variety. An algorithm
was developed to determine the genetic diversity between the
selected wheat varieties. Cluster analysis technique is used for
retrieving the results.
Abstract: Tehran, one of the heavily-populated capitals, is
severely suffering from increasing air pollution. To show a
documented trend of such pollutants during last years, plane tree
species (Platanus orientalis) were suited to be studied as indicators,
for the species have been planted throughout the city many years
ago. Two areas (Saadatabad and Narmak districts) allotting different
contents of crowed and highly-traffic routs but the same ecological
characteristics were selected. Twelve sample individuals were cored
twice perpendicularly in each area. Tree-rings of each core were
measured by a binocular microscope and separated annually for the
last 25 years. Two heavy metals including Cd and Pb accompanied
by a mineral element (Ca) were analyzed using Hatch method. Treerings
analysis of the two areas showed different groups in term of
physiologically ability as the growths were plunged during the last
10 years in Saadatabad district and showed a slight decrease in the
same period for another studying area. In direct contrast to
decreasing growth trend in Saadatabad, all three mentioned elements
increased sharply during last 25 years in the same area. When it came
to Narmak district, the trend was completely different with
Saadatabad. There were some fluctuations in absorbing trace
elements like tree-rings widths were, yet calcium showed an upward
trend all the last 25 years. The results of the study proved the
possibility of using tree species of each region to monitor its air
pollution trends of the past, hence to depict a pollution assessment of
a populated city for last years and then to make appropriate decisions
for the future as it is well-known what the trend is. On the other
hand, risen values of calcium (as the stress-indicator element)
accompanied by increased trace elements suggests non-sustainable
state of the trees.
Abstract: Zinc oxide thin films with various microstructures
were grown on substrates by using HCOOH-sols. The reaction
mechanism of the sol system was investigated by performing an XPS
analysis of as-synthesized films, due to the products of hydrolysis
and condensation in the sol system contributing to the chemical state
of the as-synthesized films. The chemical structures of the assynthesized
films related to the microstructures of the final annealed
films were also studied. The results of the Zn 2p3/2, C 1s and O1s
XPS patterns indicate that the hydrolysis reaction in the sol system is
strongly influenced by the HCOOH agent. The results of XRD and
FE-SEM demonstrated the microstructures of the annealed films are
related to the content of hydrolyzed zinc hydrate (Zn-OH) species
present, and that content of the Zn-OH species in the sol system
increases the HCOOH adding, and these Zn-OH species existing in
the sol phase are responsible for large ZnO crystallites in the final
annealed films.
Abstract: The influence of copper promoters and reaction
conditions on the formation of alcohols byproducts of a common
Fischer-Tropsch synthesis used iron-based catalysts were investigated.
A good compromise of 28%Cu/FeKLaSiO2 can lead to the
optimization of an improved Fischer-Tropsch catalyst. The product
distribution shifts towards hydrocarbons with increasing the reaction
temperature, while pressure promotes the formation of alcohols. It was
found that the production of either alcohols or hydrocarbons followed
A-S-F distributions, and their α parameters were essentially different
which indicated a competition in the growing chain between the two
species. TPD after acetaldehyde adsorption gave strong evidence of
the insertion of a C1 oxygen-containing species into an alkyl chain.
Abstract: In Croatia, the majority of cultured marine fish species are reared in net cages. The intensive production of the fish in net cages may generate the considerable amount of bio waste and change water quality especially in enclosed and semi-enclosed coastal areas. The aim of this paper is to assess the potential impact of sea bass (Dicentrarchus labrax L.) cage farm on water quality. The weak relationship between food supply and water quality parameters (nutrient content and phytoplankton biomass) was found, but significant changes in oxygen saturation was observed in the cages during the warmer period of a year especially in the morning (occasionally it dropped below 70 %). Despite of, satisfactory results of water quality parameters, it is necessary to establish comprehensive monitoring process, especially to include quality assessment of fouling communities.
Abstract: The optimal operation of proton exchange membrane fuel cell (PEMFC) requires good water management which is presented under two forms vapor and liquid. Moreover, fuel cells have to reach higher output require integration of some accessories which need electrical power. In order to analyze fuel cells operation and different species transport phenomena a biphasic mathematical model is presented by governing equations set. The numerical solution of these conservation equations is calculated by Matlab program. A multi-criteria optimization with weighting between two opposite objectives is used to determine the compromise solutions between maximum output and minimal stack size. The obtained results are in good agreement with available literature data.
Abstract: Artemisinin is a potential antimalarial drug effective
against the multidrug resistant forms of Malarial Parasites. The
current production of artemisinin is insufficient to meet the global
demand. In the present study microbial biotransformation of
arteannuin B, a biogenetic precursor of artemisinin to the later has
been investigated. Screening studies carried out on several soil borne
microorganisms have yielded one novel species with the
bioconversion ability. Crude cell free extract of 72h old culture of the
isolate had shown the bioconversion activity. On incubation with the
substrate arteannuin B, crude cell free extract of the isolate had
shown a bioconversion of 18.54% to artemisinin on molar basis with
a specific activity of 0.18 units/mg.
Abstract: Populations of wild boar present in semi-arid of central Iran. We studied features influencing bed site selection by this species in semi-arid central steppe of Iran. Habitat features of the detected bed site were compared with randomly selected by quantifying number of habitat variables in semi- arid area in Iran. The results revealed that the most important influencing factors in bed site selection were vegetation cover, number of Artemisia sieberi, percentage cover and height of Acer cinerascens, percentage cover and height of Amygdalus scoparia. This is the first ecological study of the wild boar in a protected area of the semi desert biome of Iran. Sustainability of wild boar populations in this area dependent to shrubs of Amygdalus scoparia and Acer cinerascens for thermal and camouflage cover.
Abstract: Macrophomina phaseolina is a devastating soil-borne
fungal plant pathogen that causes charcoal rot disease in many
economically important crops worldwide. So far, no registered
fungicide is available against this plant pathogen. This study was
planned to examine the antifungal activity of an allelopathic grass
Cenchrus pennisetiformis (Hochst. & Steud.) Wipff. for the
management of M. phaseolina isolated from cowpea [Vigna
unguiculata (L.) Walp.] plants suffering from charcoal rot disease.
Different parts of the plants viz. inflorescence, shoot and root were
extracted in methanol. Laboratory bioassays were carried out using
different concentrations (0, 0.5, 1.0, …, 3.0 g mL-1) of methanolic
extracts of the test allelopathic grass species to assess the antifungal
activity against the pathogen. In general, extracts of all parts of the
grass exhibited antifungal activity. All the concentrations of
methanolic extracts of shoot and root significantly reduced fungal
biomass by 20–73% and 40–80%, respectively. Methanolic shoot
extract was fractionated using n-hexane, chloroform, ethyl acetate
and n-butanol. Different concentrations of these fractions (3.125,
6.25, …, 200 mg mL-1) were analyzed for their antifungal activity.
All the concentrations of n-hexane fraction significantly reduced
fungal biomass by 15–96% over corresponding control treatments.
Higher concentrations (12.5–200 mg mL-1) of chloroform, ethyl
acetate and n-butanol also reduced the fungal biomass significantly
by 29–100%, 46–100% and 24–100%, respectively.
Abstract: Bacterial molecular chaperone DnaK plays an essential role in protein folding, stress response and transmembrane targeting of proteins. DnaKs from many bacterial species, including Escherichia coli, Salmonella typhimurium and Haemophilus infleunzae are the molecular targets for the insect-derived antimicrobial peptide pyrrhocoricin. Pyrrhocoricin-like peptides bind in the substrate recognition tunnel. Despite the high degree of crossspecies sequence conservation in the substrate-binding tunnel, some bacteria are not sensitive to pyrrhocoricin. This work addresses the molecular mechanism of resistance of Helicobacter pylori DnaK to pyrrhocoricin. Homology modelling, structural and sequence analysis identify a single aminoacid substitution at the interface between the lid and the β-sandwich subdomains of the DnaK substrate-binding domain as the major determinant for its resistance.
Abstract: In this study, we sought to investigate the mercury
removal efficiency of manganese oxides from natural gas. The
fundamental studies on mercury removal with manganese oxides
sorbents were carried out in a laboratory scale fixed bed reactor at 30
°C with a mixture of methane (20%) and nitrogen gas laden with 4.8
ppb of elemental mercury. Manganese oxides with varying surface
area and crystalline phase were prepared by conventional precipitation
method in this study. The effects of surface area, crystallinity and
other metal oxides on mercury removal efficiency were investigated.
Effect of Ag impregnation on mercury removal efficiency was also
investigated. Ag supported on metal oxide such titania and zirconia as
reference materials were also used in this study for comparison. The
characteristics of mercury removal reaction with manganese oxide
was investigated using a temperature programmed desorption (TPD)
technique.
Manganese oxides showed very high Hg removal activity (about
73-93% Hg removal) for first time use. Surface area of the manganese
oxide samples decreased after heat-treatment and resulted in complete
loss of Hg removal ability for repeated use after Hg desorption in the
case of amorphous MnO2, and 75% loss of the initial Hg removal
activity for the crystalline MnO2. Mercury desorption efficiency of
crystalline MnO2 was very low (37%) for first time use and high (98%)
after second time use. Residual potassium content in MnO2 may have
some effect on the thermal stability of the adsorbed Hg species.
Desorption of Hg from manganese oxides occurs at much higher
temperatures (with a peak at 400 °C) than Ag/TiO2 or Ag/ZrO2.
Mercury may be captured on manganese oxides in the form of mercury
manganese oxide.
Abstract: Toxoplasma gondii is an intracellular parasite capable
of infecting all nucleated cells in a diverse array of species.
Toxoplasma plaque assay have been described using Bacto Agar.
Because of its experimental advantages carboxymethyl cellulose
overlay, medium viscosity was choosing and the aim of this work
was to develop alternative method for formation of T. gondii plaques.
Tachyzoites were inoculated onto monolayers of Vero cells and
cultured at 37° C under 5 % CO2. The cultures were followed up by
microscopy inspection. Small plaques were visible by naphtol blue
stain 4 days after infection. Larger plaques could be observed by day
10 of culture. The carboxymethyl cellulose is a cheap reagent and the
methodology is easier, faster than assays under agar overlay. This is
the first description of the carboxymethyl cellulose overlay use for
obtaining the formation of T. gondii plaques and may be useful in
consequent obtaining tachyzoites for detailed studies.
Abstract: The distribution of macrobenthic polychaetes along
the coastal waters of Penang National Park was surveyed to estimate
the effect of various environmental parameters at three stations
(200m, 600m and 1200m) from the shoreline, during six sampling
months, from June 2010 to April 2011.The use of polychaetes in
descriptive ecology is surveyed in the light of a recent investigation
particularly concerning the soft bottom biota environments.
Polychaetes, often connected in the former to the notion of
opportunistic species able to proliferate after an enhancement in
organic matter, had performed a momentous role particularly with
regard to effected soft-bottom habitats. The objective of this survey
was to investigate different environment stress over soft bottom
polychaete community along Teluk Ketapang and Pantai Acheh
(Penang National Park) over a year period. Variations in the
polychaete community were evaluated using univariate and
multivariate methods. The results of PCA analysis displayed a
positive relation between macrobenthic community structures and
environmental parameters such as sediment particle size and organic
matter in the coastal water. A total of 604 individuals were examined
which was grouped into 23 families. Family Nereidae was the most
abundant (22.68%), followed by Spionidae (22.02%), Hesionidae
(12.58%), Nephtylidae (9.27%) and Orbiniidae (8.61%). It is
noticeable that good results can only be obtained on the basis of good
taxonomic resolution. We proposed that, in monitoring surveys,
operative time could be optimized not only by working at a highertaxonomic
level on the entire macrobenthic data set, but by also
choosing an especially indicative group and working at lower
taxonomic and good level.
Abstract: Ammonia nitrogen is one of the most hazardous
water pollutants, discharging into water receptors through industrial
effluents. Negative environmental impacts of such chemical species
in hydrosphere include accelerated eutrophication, water toxicity and
harming the aquatics. Natural zeolite clinoptilolite has very high
selectivity & capacity for ammonium cation sorption. It occurs in
high abundances and rich mines of this zeolite exist in different parts
of Iran and thus are available more cheaply and with different sizing.
The aim of this study is to investigate ammonia nitrogen removal
over this natural sorbent from real samples of high polluted
wastewater discharging from a fertilizer producing plant. The
experimental results showed that this natural sorbent without even
any pre treatment system & with the same particle size available in
Iranian markets has still high capability & selectivity in ammonia
nitrogen removal both in batch and continuous tests.
Abstract: A cross-sectional study was carried out to determine the prevalence, species characterization and associated risk factors with Eimeria (E.) in sheep of district Toba Tek Singh from April, 2009 to March, 2010. Of the total 486 faecal samples examined for Eimeria, 209 (43%) were found infected with five species of Eimeria. Amongst the identified species of Eimeria, E. ovinoidalis was the commonest one (48.32%), followed in order by E. ahsata, E. intricata, E. parva and E. faurei with prevalence of 45.45, 28.71, 24.40 and 19.14 percent respectively. Peak prevalence was observed in August. Wet season (rainy and post-rainy) was found to be favourable for Eimeria infection. Lambs had significantly higher prevalence (P < 0.05) of Eimeria than adults. Similarly higher prevalence of Eimeria was observed in female as compared to male. Among management and husbandry practices; watering system, housing system, floor type and herd size strongly influenced the prevalence of Eimeria. Coccidiosis was more prevalent in closed housing system, non-cemented floor type, pond watered animals and larger herds (P < 0.05) as compared to open housing system, partially cemented floor type, tap watered animals and smaller herds respectively. Feeding system, breed and body condition of animals were not found as risk factors (P>0.05) influencing prevalence of Eimeria.
Abstract: Generation of electricity from coal has increased over
the years in the United States and around the world. Burning of coal
results in annual production of upwards of 100 millions tons (United
States only) of coal combustion products (CCPs). Only about a third
of these products are being used to create new products while the
remainder goes to landfills. Application of CCPs mixed with
composted organic materials onto soil can improve the soil-s
physico-chemical conditions and provide essential plant nutritients.
Our objective was to create plant growth media utilizing CCPs and
compost in way which maximizes the use of these products and, at
the same time, maintain good plant growth. Media were formulated
by adding composted organic matter (COM) to CCPs at ratios
ranging from 2:8 to 8:2 (v/v). The quality of these media was
evaluated by measuring their physical and chemical properties and
their effect on plant growth. We tested the media by 1) measuring
their physical and chemical properties and 2) the growth of three
plant species in the experimental media: wheat (Triticum sativum),
tomato (Lycopersicum esculentum) and marigold (Tagetes patula).
We achieved significantly (p < 0.001) higher growth (7-130%) in the
experimental media containing CCPs compared to a commercial mix.
The experimental media supplied adequate plant nutrition as no
fertilization was provided during the experiment. Based on the
results, we recommend the use of CCPs and composts for the
creation of plant growth media.
Abstract: In situ observation of absorption spectral change of
heptil viologen cation radical (HV+.) was performed by slab optical
waveguide (SOWG) spectroscopy utilizing indium-tin-oxide (ITO)
electrodes. Synchronizing with electrochemical techniques, we
observed the adsorption process of HV+.on the ITO electrode. In this
study, we carried out the ITO-SOWG observations using KBr aqueous
solution containing different concentration of HV to investigate the
concentration dependent spectral change. A few specific absorption
bands, which indicated HV+.existed as both monomer and dimer on
ITO electrode surface with a monolayer or a few layers deposition,
were observed in UV-visible region. The change in the peak position
of the absorption spectra from adsorption species of HV+. were
correlated with the concentration of HV as well as the electrode
potential.
Abstract: In this paper, the existence of 2n positive periodic solutions for n species non-autonomous Lotka-Volterra cooperative systems with harvesting terms is established by using Mawhin-s continuation theorem of coincidence degree theory and matrix inequality. An example is given to illustrate the effectiveness of our results.
Abstract: Neem is a highly heterozygous and commercially
important perennial plant. Conventionally, it is propagated by seeds
which loose viability within two weeks. Strictly cross pollinating
nature of the plant causes serious barrier to the genetic improvement
by conventional methods. Alternative methods of tree improvement
such as somatic hybridization, mutagenesis and genetic
transformation require an efficient in vitro plant regeneration system.
In this regard, somatic embryogenesis particularly secondary somatic
embryogenesis may offer an effective system for large scale plant
propagation without affecting the clonal fidelity of the regenerants. It
can be used for synthetic seed production, which further bolsters
conservation of this tree species which is otherwise very difficult
The present report describes the culture conditions necessary to
induce and maintain repetitive somatic embryogenesis, for the first
time, in neem. Out of various treatments tested, the somatic embryos
were induced directly from immature zygotic embryos of neem on
MS + TDZ (0.1 μM) + ABA (4 μM), in more than 76 % cultures.
Direct secondary somatic embryogenesis occurred from primary
somatic embryos on MS + IAA (5 μM) + GA3 (5 μM) in 12.5 %
cultures. Embryogenic competence of the explant as well as of the
primary embryos was maintained for a long period by repeated
subcultures at frequent intervals. A maximum of 10 % of these
somatic embryos were converted into plantlets.