Abstract: Highly pathogenic avian influenza (HPAI) H5N1 viruses have created demand for a cost-effective vaccine to prevent a pandemic of the disease. Here, we report that Trichoplusia ni (T. ni) larvae can act as a cost-effective bioreactor to produce recombinant HA5 (rH5HA) proteins as an potential effective vaccine for chickens. To facilitate the recombinant virus identification, virus titer determination and access the infected larvae, we employed the internal ribosome entry site (IRES) derived from Perina nuda virus (PnV, belongs to insect picorna like Iflavirus genus) to construct a bi-cistronic baculovirus expression vector that can express the rH5HA protein and enhanced green fluorescent protein (EGFP) simultaneously. Western blot analysis revealed that the 70 kDa rH5HA protein and partially cleaved products (40 kDa H5HA1) were generated in T. ni larvae infected with recombinant baculovirus carrying the H5HA gene. These data suggest that the baculovirus-larvae recombinant protein expression system could be a cost-effective platform for H5N1 vaccine production.
Abstract: Due to short product life cycles, increasing variety of
products and short cycles of leap innovations manufacturing
companies have to increase the flexibility of factory structures.
Flexibility of factory structures is based on defined factory planning
processes in which product, process and resource data of various
partial domains have to be considered. Thus factory planning
processes can be characterized as iterative, interdisciplinary and
participative processes [1]. To support interdisciplinary and
participative character of planning processes, a federative factory
data management (FFDM) as a holistic solution will be described.
FFDM is already implemented in form of a prototype. The interim
results of the development of FFDM will be shown in this paper. The
principles are the extracting of product, process and resource data
from documents of various partial domains providing as web services
on a server. The described data can be requested by the factory
planner by using a FFDM-browser.
Abstract: A new decomposition form is introduced in this report
to establish a criterion for the bi-partite separability of Bell diagonal
states. A such criterion takes a quadratic inequality of the coefficients
of a given Bell diagonal states and can be derived via a simple
algorithmic calculation of its invariants. In addition, the criterion can
be extended to a quantum system of higher dimension.
Abstract: Developing a university course schedule is
difficult. This is due to the limitations in the resources
available. The process is made even harder with different
faculties or departments having different ways of stating their
schedule requirements. The person in charge of taking the
schedule requirements and turning them into a proper course
schedule is not only burden with the task of allocating the
appropriate classes and time to lecturers and students, they
also need to understand the schedule requirements. Therefore
a scheduling support system named SATA is developed to
assist ICRESS in the course scheduling process. SATA has
been put to use for several semesters and the results have been
encouraging. It won a bronze medal in the 2008 Invention,
Innovation and Design competition (IID-08) and has been
submitted to be patented in October 2008
Abstract: Functional gastrointestinal disorders (FGID) affect millions of people spread all age regardless of race and sex. Emotional stress and obesity have been associated with increased reporting of gastrointestinal (GI) symptoms, but the relationship between FGID and risk factors (emotional stress or obesity) is unclear. Our aim was to assess the changes of the mechanical characteristics on the gastrointestinal tracts of the mentally fatigued obese and normal rat models. Finally, using the physical characteristics with micro-indentation test, we made a close investigation into the relation between FGID and risk factors quantitatively.
Abstract: Training with Functional Electrical Stimulation (FES)
has both physiological and psychological benefits for spinal cord
injured subjects. Commonly used methods for quantification of
spasticity have shown controversial reliability. In this study we
propose a method for quick determination of spasticity in spinal cord
injured subjects on a cycling and measurement system. 23 patients
did training sessions on an instrumented mobile FES cycle three
times a week over two months as part of their clinical rehabilitation
program. Spasticity (MAS) and the legs resistance to the pedaling
motion were assessed before and after the FES training and
measurements were done on the subjects ability to pedal with our
without motor assistance. Measurements with test persons with
incomplete spastic paraplegia have shown that spasticity is decreased
after a 30 min cycling training with functional electrical stimulation
(FES).
Abstract: Pepper spray use has gained momentum since 1992
and although the active ingredient is readily available, it is considered
a weapon with restricted use in many regions, including The
Bahamas. In light of controversy in the literature regarding the
severity of presenting respiration complaints among individuals postacute
exposure of pepper spray inhalation, this descriptive case series
study was conducted to assess the respiratory status of persons
evaluated during a mass casualty in The Bahamas. Parameters noted
were patients- demographics and respiration severity determined via
clinical examination findings, disposition and follow-up review of the
20 persons. Their most common complaint was difficulty breathing
post exposure. Two required admission and stayed for
Abstract: A wireless power transfer system can attribute to the
fields in robot, aviation and space in which lightening the weight of
device and improving the movement play an important role. A
wireless power transfer system was investigated to overcome the
inconvenience of using power cable. Especially a wireless power
transfer technology is important element for mobile robots. We
proposed the wireless power transfer system of the half-bridge
resonant converter with the frequency tracking and optimized
power transfer control unit. And the possibility of the application
and development system was verified through the experiment with
LED loads.
Abstract: The paper investigates the main issues concerning the activity of mergers and acquisitions in Romania in the period 2000- 2004. As in the first decade after the failure of the socialist economy the market of M&A was living its infancy based exclusively on privatization transactions, after 2000 the market entered in a stage of maturity. We are investigated both the registered evolutions in terms of number and value of transactions, and some sectoral issues, concerning the most important industries affected by the M&A process.
Abstract: Component-Based software engineering provides an
opportunity for better quality and increased productivity in software
development by using reusable software components [10]. One of the
most critical aspects of the quality of a software system is its
performance. The systematic application of software performance
engineering techniques throughout the development process can help
to identify design alternatives that preserve desirable qualities such
as extensibility and reusability while meeting performance objectives
[1]. In the present scenario, software engineering methodologies
strongly focus on the functionality of the system, while applying a
“fix- it-later" approach to software performance aspects [3]. As a
result, lengthy fine-tunings, expensive extra hard ware, or even
redesigns are necessary for the system to meet the performance
requirements. In this paper, we propose design based,
implementation independent, performance prediction approach to
reduce the overhead associated in the later phases while developing a
performance guaranteed software product with the help of Unified
Modeling Language (UML).
Abstract: In this paper, stability and Hopf bifurcation analysis of
a novel hyperchaotic system are investigated. Four feedback control
strategies, the linear feedback control method, enhancing feedback
control method, speed feedback control method and delayed feedback
control method, are used to control the hyperchaotic attractor to
unstable equilibrium. Moreover numerical simulations are given to
verify the theoretical results.
Abstract: This paper discusses the effectiveness of the EEG signal
for human identification using four or less of channels of two different
types of EEG recordings. Studies have shown that the EEG signal
has biometric potential because signal varies from person to person
and impossible to replicate and steal. Data were collected from 10
male subjects while resting with eyes open and eyes closed in 5
separate sessions conducted over a course of two weeks. Features
were extracted using the wavelet packet decomposition and analyzed
to obtain the feature vectors. Subsequently, the neural networks
algorithm was used to classify the feature vectors. Results show that,
whether or not the subjects- eyes were open are insignificant for a 4–
channel biometrics system with a classification rate of 81%. However,
for a 2–channel system, the P4 channel should not be included if data
is acquired with the subjects- eyes open. It was observed that for 2–
channel system using only the C3 and C4 channels, a classification
rate of 71% was achieved.
Abstract: Extraction of lactic acid by emulsion liquid membrane technology (ELM) using n-trioctyl amine (TOA) in n-heptane as carrier within the organic membrane along with sodium carbonate as acceptor phase was optimized by using response surface methodology (RSM). A three level Box-Behnken design was employed for experimental design, analysis of the results and to depict the combined effect of five independent variables, vizlactic acid concentration in aqueous phase (cl), sodium carbonate concentration in stripping phase (cs), carrier concentration in membrane phase (ψ), treat ratio, and batch extraction time (τ)
with equal volume of organic and external aqueous phase on lactic acid extraction efficiency. The maximum lactic acid extraction efficiency (ηext) of 98.21%from aqueous phase in a batch reactor using ELM was found at the optimized values for test variables, cl, cs, ψ, and τ as 0.06 [M], 0.18 [M], 4.72 (%,v/v), 1.98 (v/v) and 13.36 min respectively.
Abstract: This research analyzes factors affecting the success of
Litecoin Value within Thailand and develops a guideline for selfreliance
for effective business implementation. Samples in this study
included 119 people through surveys. The results revealed four main
factors affecting the success as follows: 1) Future Career training
should be pursued in applied Litecoin development. 2) Didn't grasp
the concept of a digital currency or see the benefit of a digital
currency. 3) There is a great need to educate the next generation of
learners on the benefits of Litecoin within the community. 4) A great
majority didn't know what Litecoin was.
The guideline for self-reliance planning consisted of 4 aspects: 1)
Development planning: by arranging meet up groups to conduct
further education on Litecoin and share solutions on adoption into
every day usage. Local communities need to develop awareness of
the usefulness of Litecoin and share the value of Litecoin among
friends and family. 2) Computer Science and Business Management
staff should develop skills to expand on the benefits of Litecoin
within their departments. 3) Further research should be pursued on
how Litecoin Value can improve business and tourism within
Thailand. 4) Local communities should focus on developing Litecoin
awareness by encouraging street vendors to accept Litecoin as
another form of payment for services rendered.
Abstract: This paper describes dynamic analysis using proposed
fast finite element method for a shock absorbing structure including a
sponge. The structure is supported by nonlinear concentrated springs.
The restoring force of the spring has cubic nonlinearity and linear
hysteresis damping. To calculate damping properties for the structures
including elastic body and porous body, displacement vectors as
common unknown variable are solved under coupled condition. Under
small amplitude, we apply asymptotic method to complex eigenvalue
problem of this system to obtain modal parameters. And then
expressions of modal loss factor are derived approximately. This
approach was proposed by one of the authors previously. We call this
method as Modal Strain and Kinetic Energy Method (MSKE method).
Further, using the modal loss factors, the discretized equations in
physical coordinate are transformed into the nonlinear ordinary
coupled equations using normal coordinate corresponding to linear
natural modes. This transformation yields computation efficiency. As
a numerical example of a shock absorbing structures, we adopt double
skins with a sponge. The double skins are supported by nonlinear
concentrated springs. We clarify influences of amplitude of the input
force on nonlinear and chaotic responses.
Abstract: Sign language is used by the deaf and hard of hearing people for communication. Automatic sign language recognition is a challenging research area since sign language often is the only way of communication for the deaf people. Sign language includes different components of visual actions made by the signer using the hands, the face, and the torso, to convey his/her meaning. To use different aspects of signs, we combine the different groups of features which have been extracted from the image frames recorded directly by a stationary camera. We combine the features in two levels by employing three techniques. At the feature level, an early feature combination can be performed by concatenating and weighting different feature groups, or by concatenating feature groups over time and using LDA to choose the most discriminant elements. At the model level, a late fusion of differently trained models can be carried out by a log-linear model combination. In this paper, we investigate these three combination techniques in an automatic sign language recognition system and show that the recognition rate can be significantly improved.
Abstract: The objective of this work is to produce heterotrophic
microalgal lipid in flask-batch fermentation. Chlorella sp. KKU-S2
supported maximum values of 0.374 g/L/d, 0.478 g lipid/g cells, and
0.112 g/L/d for volumetric lipid production rate, and specific yield of
lipid, and specific rate of lipid production, respectively when culture
was performed on BG-11 medium supplemented with 50g/L glucose.
Among the carbon sources tested, maximum cell yield coefficient
(YX/S, g/L), maximum specific yield of lipid (YP/X, g lipid/g cells) and
volumetric lipid production rate (QP, g/L/d) were found of 0.728,
0.237, and 0.619, respectively, using sugarcane molasses as carbon
source. The main components of fatty acid from extracted lipid were
palmitic acid, stearic acid, oleic acid and linoleic acid which similar
to vegetable oils and suitable for biodiesel production.
Abstract: The usual method of river flow diversion involves construction of tunnels and cofferdams. Given the fact that the cost of diversion works could be as high as 10-20% of the total dam construction cost, due attention should be paid to optimum design of the diversion works. The cost of diversion works depends, on factors, such as: the tunnel dimensions and the intended tunneling support measures during and after excavation; quality and characterizes of the rock through which the tunnel should be excavated; the dimensions of the upstream (and downstream) cofferdams; and the magnitude of river flood the system is designed to divert. In this paper by use of the cost of unit prices for tunnel excavation, tunnel lining, tunnel support (rock bolt + shotcrete) and cofferdam fill the cost function was determined. The function is then minimized by the aid of PSO Algorithm (particle swarm optimization). It is found that the optimum diameter and the total diversion cost are directly related to the river flood discharge (Q). It has also shown that in addition to optimum diameter design discharge (Q), river length, tunnel length, is mainly a function of the ratios (not the absolute values) of the unit prices and does not depend on the overall price levels in the respective country. The results of optimization use in some of the case study lead us to significant changes in the cost.
Abstract: To investigate the production of cellulases from Aspergillus heteromorphus, submerged fermentation was performed using wheat straw as substrate. Optimization of saccharification conditions like pH, temperature and time were studied. Highest reducing sugar was released on 5th day at 5 pH, 30° C temperature. When A. heteromorphous was grown on wheat straw in submerged fermentation after 5 days incubation at 30 ° C, 3.2 IU/ml and 83 IU/ml, filter paper activity and CMCase activity respectively.
Abstract: Hand gesture is an active area of research in the vision
community, mainly for the purpose of sign language recognition and
Human Computer Interaction. In this paper, we propose a system to
recognize alphabet characters (A-Z) and numbers (0-9) in real-time
from stereo color image sequences using Hidden Markov Models
(HMMs). Our system is based on three main stages; automatic segmentation
and preprocessing of the hand regions, feature extraction
and classification. In automatic segmentation and preprocessing stage,
color and 3D depth map are used to detect hands where the hand
trajectory will take place in further step using Mean-shift algorithm
and Kalman filter. In the feature extraction stage, 3D combined features
of location, orientation and velocity with respected to Cartesian
systems are used. And then, k-means clustering is employed for
HMMs codeword. The final stage so-called classification, Baum-
Welch algorithm is used to do a full train for HMMs parameters.
The gesture of alphabets and numbers is recognized using Left-Right
Banded model in conjunction with Viterbi algorithm. Experimental
results demonstrate that, our system can successfully recognize hand
gestures with 98.33% recognition rate.