Production of Cellulases by Aspergillus Heteromorphus from Wheat Straw under Submerged Fermentation
To investigate the production of cellulases from Aspergillus heteromorphus, submerged fermentation was performed using wheat straw as substrate. Optimization of saccharification conditions like pH, temperature and time were studied. Highest reducing sugar was released on 5th day at 5 pH, 30° C temperature. When A. heteromorphous was grown on wheat straw in submerged fermentation after 5 days incubation at 30 ° C, 3.2 IU/ml and 83 IU/ml, filter paper activity and CMCase activity respectively.
[1] Singh, R., Singh, N., Parkash, A., Poonia, S., 2006. Saccharification
studies by a thermophilic fungus Sporotricum thermophile isolated from
agriculture waste of Bhopal, Indian Journal of Environment &
Ecoplanning, 12 (1) 97-104
[2] Kuhad RC, Singh A, Eriksson KE (1997). Microorganisms enzymes
involved in the degradation of plant fiber cell walls. Adv. Biochem. Eng.
Biotechnol. 57: 45-125
[3] Reith, J. H., den Uil, H., van Veen, H., de Laat WTAM, Niessen, J. J. &
de Jong, E. (2002). Coproduction of bioethanol, electricity and heat from
biomass residues. 12th European Conference and Technology Exhibition
on Biomass from Energy, Industry and Climate Protection, Amsterdam,
The Netherlands, 17-21 June.
[4] Wen, Z., Liao, W., & Chen, S. (2005). Production of cellulase/bglucosidase
by the mixed fungi culture Trichoderma reesei and
Aspergillus phoenicis on dairy manure. Process Biochemistry, 40, 3087-
3094.
[5] Dale BE (1999). Biobased industrial products: bioprocess engineering
when cost really counts. Biotechnol. Prog. 15: 775-776.
[6] Lynd LR, Wyman CE, Gerngross TU (1999). Biocommodity
engineering. Biotechnol. Program. 15: 777-793.
[7] Gubitz, G.M., Mansfield, S.D., Bohm, D., Saddler, J.N., 1998. Effect of
endoglucanases and hemicellulases in magnetic and flotation deinking of
xerographic and laser-printed papers. J. Biotechnol. 65, 209-215.
[8] Bajpai, P., 1999. Application of enzymes in the pulp and paper industry.
Biotechnol. Prog. 15, 147-157.
[9] Sternberg D., 1976, Production of Cellulase by Trichoderma. Biotech
Bioeng. Symp. (6), 35-53.
[10] Miller GL (1959) Use of dinitrosalicyclic acid reagent for determination
of reducing sugar. Analytical Chemistry 31: 426-428
[11] Ghose, T. K. (1987). Measurement of cellulase activities. Pure &
Applied Chemistry 59: 257-268
[12] Baig MMV, Baig MLB, Baig MIA & Yasmeen M (2004)
Saccharification of banana agro-waste by cellulolytic enzymes. African
Journal of Biotechnology 3 (9): 447-450
[13] Khan MMH, Ali S, Razi AF & Alzam MZ (2007) Use of fungi for the
bioconversion of rice straw into cellulose enzyme. Journal of
Environmental Science and Health Part B. 42: 381-386.
[14] Narasimha G, Sridevi A, Viswanath, B., Chandra M. S., Reddy, R.B.
2006 Nutrient effects on production of cellulolytic enzymes by
Aspergillus niger. African Journal of Biotechnology 5 (5), 472-476
[15] Muthuvelayudham, R and Viruthagir, 2006 Fermentative production and
kinetics of cellulose protein on Tricoderma reesei ysing sugarcane
bagasse and rice straw. African Journal of Biotechnology Vol. 5 (20),
pp. 1873-1881, 16 october 2006
[16] Kang, S.W., Park, Y.S., Lee, J.S. , Hong , S.I., Kim, S.W., 2004.
Production of cellulases and hemicellulases by Aspergillus niger KK2
from lignocellulosic biomass, Bioresource Technology, 91, 153-156
[17] Pandey A, Selvakumar P, Soccol CR & Nigam P (1999) Solid State
Cultivation for the Production of Industrial Enzymes. Current Sci. 77:
149-162
[18] Ojumu TV, Solomon B O, Betiku E, Layokun SK & Amigun B (2003)
Cellulase Production by Aspergillus flavus Linn Isolate NSPR 101
fermented in sawdust, bagasse and corncob. African Journal of
Biotechnology 2 (6): 150-152.
[19] Alam MZ, Muhammad N & Mahmat ME (2005) Production of cellulase
from oil palm biomass as substrate by solid state bioconversion.
American Journal of Applied Science 2(2):569-572
[20] Hattaka AI (1983) Biological pretreatment of lignocellulose. Applied
Microbiol. Biotechnol. 18:350-357.
[21] Considine PJ, O-Rorke A, Hackett TJ & Coughlan MP (1988)
Hydrolysis of beet pulp polysaccharides by extracts if solid state cultures
of Penicillium capsulatum. Biotechnol. Bioeng. 31:433- 438.
[22] Gonzalez G, Caminal G & Lopez-Sontin (1986) Cellulase production on
lignocellulose materials and performance of a packed-red reactor for
enzymatic hydrolysis. Biotechnol. Bioeng. Symp. 17: 535-542.
[23] Okeke BC & Obi SKC (1995) Saccharification of agro-waste materials
by fungal cellulases and hemicellulases. Biores. Technol. 51: 23-27.
[1] Singh, R., Singh, N., Parkash, A., Poonia, S., 2006. Saccharification
studies by a thermophilic fungus Sporotricum thermophile isolated from
agriculture waste of Bhopal, Indian Journal of Environment &
Ecoplanning, 12 (1) 97-104
[2] Kuhad RC, Singh A, Eriksson KE (1997). Microorganisms enzymes
involved in the degradation of plant fiber cell walls. Adv. Biochem. Eng.
Biotechnol. 57: 45-125
[3] Reith, J. H., den Uil, H., van Veen, H., de Laat WTAM, Niessen, J. J. &
de Jong, E. (2002). Coproduction of bioethanol, electricity and heat from
biomass residues. 12th European Conference and Technology Exhibition
on Biomass from Energy, Industry and Climate Protection, Amsterdam,
The Netherlands, 17-21 June.
[4] Wen, Z., Liao, W., & Chen, S. (2005). Production of cellulase/bglucosidase
by the mixed fungi culture Trichoderma reesei and
Aspergillus phoenicis on dairy manure. Process Biochemistry, 40, 3087-
3094.
[5] Dale BE (1999). Biobased industrial products: bioprocess engineering
when cost really counts. Biotechnol. Prog. 15: 775-776.
[6] Lynd LR, Wyman CE, Gerngross TU (1999). Biocommodity
engineering. Biotechnol. Program. 15: 777-793.
[7] Gubitz, G.M., Mansfield, S.D., Bohm, D., Saddler, J.N., 1998. Effect of
endoglucanases and hemicellulases in magnetic and flotation deinking of
xerographic and laser-printed papers. J. Biotechnol. 65, 209-215.
[8] Bajpai, P., 1999. Application of enzymes in the pulp and paper industry.
Biotechnol. Prog. 15, 147-157.
[9] Sternberg D., 1976, Production of Cellulase by Trichoderma. Biotech
Bioeng. Symp. (6), 35-53.
[10] Miller GL (1959) Use of dinitrosalicyclic acid reagent for determination
of reducing sugar. Analytical Chemistry 31: 426-428
[11] Ghose, T. K. (1987). Measurement of cellulase activities. Pure &
Applied Chemistry 59: 257-268
[12] Baig MMV, Baig MLB, Baig MIA & Yasmeen M (2004)
Saccharification of banana agro-waste by cellulolytic enzymes. African
Journal of Biotechnology 3 (9): 447-450
[13] Khan MMH, Ali S, Razi AF & Alzam MZ (2007) Use of fungi for the
bioconversion of rice straw into cellulose enzyme. Journal of
Environmental Science and Health Part B. 42: 381-386.
[14] Narasimha G, Sridevi A, Viswanath, B., Chandra M. S., Reddy, R.B.
2006 Nutrient effects on production of cellulolytic enzymes by
Aspergillus niger. African Journal of Biotechnology 5 (5), 472-476
[15] Muthuvelayudham, R and Viruthagir, 2006 Fermentative production and
kinetics of cellulose protein on Tricoderma reesei ysing sugarcane
bagasse and rice straw. African Journal of Biotechnology Vol. 5 (20),
pp. 1873-1881, 16 october 2006
[16] Kang, S.W., Park, Y.S., Lee, J.S. , Hong , S.I., Kim, S.W., 2004.
Production of cellulases and hemicellulases by Aspergillus niger KK2
from lignocellulosic biomass, Bioresource Technology, 91, 153-156
[17] Pandey A, Selvakumar P, Soccol CR & Nigam P (1999) Solid State
Cultivation for the Production of Industrial Enzymes. Current Sci. 77:
149-162
[18] Ojumu TV, Solomon B O, Betiku E, Layokun SK & Amigun B (2003)
Cellulase Production by Aspergillus flavus Linn Isolate NSPR 101
fermented in sawdust, bagasse and corncob. African Journal of
Biotechnology 2 (6): 150-152.
[19] Alam MZ, Muhammad N & Mahmat ME (2005) Production of cellulase
from oil palm biomass as substrate by solid state bioconversion.
American Journal of Applied Science 2(2):569-572
[20] Hattaka AI (1983) Biological pretreatment of lignocellulose. Applied
Microbiol. Biotechnol. 18:350-357.
[21] Considine PJ, O-Rorke A, Hackett TJ & Coughlan MP (1988)
Hydrolysis of beet pulp polysaccharides by extracts if solid state cultures
of Penicillium capsulatum. Biotechnol. Bioeng. 31:433- 438.
[22] Gonzalez G, Caminal G & Lopez-Sontin (1986) Cellulase production on
lignocellulose materials and performance of a packed-red reactor for
enzymatic hydrolysis. Biotechnol. Bioeng. Symp. 17: 535-542.
[23] Okeke BC & Obi SKC (1995) Saccharification of agro-waste materials
by fungal cellulases and hemicellulases. Biores. Technol. 51: 23-27.
@article{"International Journal of Biological, Life and Agricultural Sciences:50898", author = "Anita Singh and Namita Singh and Narsi R. Bishnoi", title = "Production of Cellulases by Aspergillus Heteromorphus from Wheat Straw under Submerged Fermentation", abstract = "To investigate the production of cellulases from Aspergillus heteromorphus, submerged fermentation was performed using wheat straw as substrate. Optimization of saccharification conditions like pH, temperature and time were studied. Highest reducing sugar was released on 5th day at 5 pH, 30° C temperature. When A. heteromorphous was grown on wheat straw in submerged fermentation after 5 days incubation at 30 ° C, 3.2 IU/ml and 83 IU/ml, filter paper activity and CMCase activity respectively.
", keywords = "Aspergillus heteromorphus, Wheat Straw,
Submerged Fermentation, Production of Cellulases", volume = "3", number = "3", pages = "130-4", }
