Abstract: Methicillin/multiple-resistant Staphylococcus aureus
(MRSA) are infectious bacteria that are resistant to common
antibiotics. A previous in silico study in our group has identified a
hypothetical protein SAV1226 as one of the potential drug targets. In
this study, we reported the bioinformatics characterization, as well as
cloning, expression, purification and kinetic assays of hypothetical
protein SAV1226 from methicillin/vancomycin-resistant
Staphylococcus aureus Mu50 strain. MALDI-TOF/MS analysis
revealed a low degree of structural similarity with known proteins.
Kinetic assays demonstrated that hypothetical protein SAV1226 is
neither a domain of an ATP dependent dihydroxyacetone kinase nor
of a phosphotransferase system (PTS) dihydroxyacetone kinase,
suggesting that the function of hypothetical protein SAV1226 might
be misannotated on public databases such as UniProt and
InterProScan 5.
Abstract: In the past decade, the use of digital image correlation
(DIC) techniques has increased significantly in the area of
experimental mechanics, especially for materials behavior
characterization. This non-contact tool enables full field displacement
and strain measurements over a complete region of interest. The DIC
algorithm requires a random contrast pattern on the surface of the
specimen in order to perform properly. To create this pattern, the
specimen is usually first coated using a white matt paint. Next, a
black random speckle pattern is applied using any suitable method. If
the applied paint coating is too thick, its top surface may not be able
to exactly follow the deformation of the specimen, and consequently,
the strain measurement might be underestimated. In the present
article, a study of the influence of the paint thickness on the strain
underestimation is performed for different strain levels. The results
are then compared to typical paint coating thicknesses applied by
experienced DIC users. A slight strain underestimation was observed
for paint coatings thicker than about 30μm. On the other hand, this
value was found to be uncommonly high compared to coating
thicknesses applied by DIC users.
Abstract: In the present research work we present the optical
emission studies of the Indium (In) – Tin (Sn) plasma produced by
the first (1064 nm) harmonic of an Nd: YAG nanosecond pulsed
laser. The experimentally observed line profiles of neutral Indium (In
I) and Tin (SnI) are used to extract the electron temperature (Te)
using the Boltzmann plot method. Whereas, the electron number
density (Ne) has been determined from the Stark broadening line
profile method. The Te is calculated by varying the distance from the
target surface along the line of propagation of plasma plume and also
by varying the laser irradiance. Beside we have studied the variation
of Ne as a function of laser irradiance as well as its variation with
distance from the target surface.
Abstract: Development of new generation bio-tribological,
multilayer coatings opens an avenue for fabrication of future hightech
functional surfaces. In the presented work, nano-composite,
Cr/CrN+[Cr/ a-C:H implanted by metallic nanocrystals] multilayer
coatings have been developed for surface protection of medical tools.
Thin films were fabricated by a hybrid Pulsed Laser Deposition
technique. Complex microstructure analysis of nanomultilayer
coatings, subjected to mechanical and biological tests, were
performed by means of transmission electron microscopy (TEM).
Microstructure characterization revealed the layered arrangement of
Cr23C6 nanoparticles in multilayer structure. Influence of deposition
conditions on bio-tribological properties of the coatings was studied.
The bio-tests were used as a screening tool for the analyzed
nanomultilayer coatings before they could be deposited on medical
tools. Bio-medical tests were done using fibroblasts. The mechanical
properties of the coatings were investigated by means of a ball-ondisc
mechanical test. The micro hardness was done using Berkovich
indenter. The scratch adhesion test was done using Rockwell
indenter. From the bio-tribological point of view, the optimal
properties had the C106_1 material.
Abstract: Community integration is a construct that an
increasing body of research has shown to have a significant impact
on the wellbeing and recovery of people with psychiatric problems.
However, there are few studies that explore which factors can be
associated and predict community integration. Moreover, community
integration has been mostly studied in minority groups, and current
literature on the definition and manifestation of community
integration in the general population is scarcer. Thus, the current
study aims to characterize community integration and explore
possible predictor variables in a sample of participants with
psychiatric problems (PP, N=183) and a sample of participants from
the general population (GP, N=211).
Results show that people with psychiatric problems present above
average values of community integration, but are significantly lower
than their healthy counterparts. It was also possible to observe that
community integration does not vary in terms of the sociodemographic
characteristics of both groups in this study. Correlation
and multiple regression showed that, among several variables that
literature present as relevant in the community integration process,
only three variables emerged as having the most explanatory value in
community integration of both groups: sense of community, basic
needs satisfaction and submission. These results also shown that
those variables have increased explanatory power in the PP sample,
which leads us to emphasize the need to address this issue in future
studies and increase the understanding of the factors that can be
involved in the promotion of community integration, in order to
devise more effective interventions in this field.
Abstract: Paranoid ideation is a common thought process that
constitutes a defense against perceived social threats. The current
study aimed at the characterization of paranoid ideation in youths and
to explore the possible predictors involved in the development of
paranoid ideations. Paranoid ideation, shame, submission, early
childhood memories and current depressive, anxious and stress
symptomatology were assessed in a sample of 1516 Portuguese
youths. Higher frequencies of paranoid ideation were observed,
particularly in females and youths from lower socioeconomic status.
The main predictors identified relates to submissive behaviors and
adverse childhood experiences, and especially to shame feelings. The
current study emphasizes that the these predictors are similar to
findings in adults and clinical populations, and future implications to
research and clinical practice aiming at paranoid ideations are
discussed, as well as the pertinence of the study of mediating factors
that allow a wider understanding of this thought process in younger
populations and the prevention of psychopathology in adulthood.
Abstract: Optical biosensors have become a powerful detection
and analysis tool for wide-ranging applications in biomedical research,
pharmaceuticals and environmental monitoring. This study carried out
the computational fluid dynamics (CFD)-based simulations to explore
the dispersion phenomenon in the micro channel of an optical
biosensor. The predicted time sequences of concentration contours
were utilized to better understand the dispersion development occurred
in different geometric shapes of micro channels. The simulation results
showed the surface concentrations at the sensing probe (with the best
performance of a grating coupler) in respect of time to appraise the
dispersion effect and therefore identify the design configurations
resulting in minimum dispersion.
Abstract: This paper presents the design and characterization of
analog readout interface circuits for ion sensitive field effect
transistor (ISFET) and ion selective electrode (ISE) based sensor.
These interface circuits are implemented using MIMOS’s 0.35um
CMOS technology and experimentally characterized under 24-leads
QFN package. The characterization evaluates the circuit’s
functionality, output sensitivity and output linearity. Commercial
sensors for both ISFET and ISE are employed together with glass
reference electrode during testing. The test result shows that the
designed interface circuits manage to readout signals produced by
both sensors with measured sensitivity of ISFET and ISE sensor are
54mV/pH and 62mV/decade, respectively. The characterized output
linearity for both circuits achieves above 0.999 Rsquare. The readout
also has demonstrated reliable operation by passing all qualifications
in reliability test plan.
Abstract: Intermetallic materials are among advanced
technology materials that have outstanding mechanical and physical
properties for high temperature applications. Especially creep
resistance, low density and high hardness properties stand out in such
intermetallics. The microstructure, mechanical properties of %88Ni-
%10Cr and %2Mn powders were investigated using specimens
produced by tube furnace sintering at 900-1300°C temperature. A
composite consisting of ternary additions, a metallic phase, Fe, Cr
and Mn have been prepared under Ar shroud and then tube furnace
sintered. XRD, SEM (Scanning Electron Microscope), were
investigated to characterize the properties of the specimens.
Experimental results carried out for composition %88Ni-%10Cr and
%2Mn at 1300°C suggest that the best properties as 138,80HV and
6,269/cm3 density were obtained at 1300°C.
Abstract: Sol-enhanced Zn-Ni-Al2O3 nanocomposite coatings
were electroplated on mild steel by our newly developed solenhanced
electroplating method. In this method, transparent Al2O3 sol
was added into the acidic Zn-Ni bath to produced Zn-Ni-Al2O3nanocomposite
coatings. The chemical composition, microstructure and
mechanical properties of the composite and alloy coatings deposited
at two different agitation speed were investigated. The structure of all
coatings was single γ-Ni5Zn21 phase. The composite coatings possess
refined crystals with higher microhardness compared to Zn-Ni alloy
coatings. The wear resistance of Zn-Ni coatings was improved
significantly by incorporation of alumina nano particles into the
coatings. Higher agitation speed provided more uniform coatings
with smaller grain sized and slightly higher microhardness.
Considering composite coatings, high agitation speeds may facilitate
co-deposition of alumina in the coatings.
Abstract: Brass terminal, one of the several crude oil and
petroleum products storage/handling facilities in the Niger Delta was
built in the 1980s. Activities at this site, over the years, released
crude oil into this 3 m-deep, 1500 m-long canal lying adjacent to the
terminal with oil floating on it and its sediment heavily polluted. To
ensure effective clean-up, three major activities were planned: site
characterization, bioremediation pilot plant construction and testing
and full-scale bioremediation of contaminated sediment / bank soil by
land farming. The canal was delineated into 12 lots and each
characterized, with reference to the floating oily phase, contaminated
sediment and canal bank soil. As a result of site characterization, a
pilot plant for on-site bioremediation was designed and a treatment
basin constructed for carrying out pilot bioremediation test.
Following a designed sampling protocol, samples from this pilot
plant were collected for analysis at two laboratories as a quality
assurance / quality control check. Results showed that Brass Canal
upstream is contaminated with dark, thick and viscous oily film with
characteristic hydrocarbon smell while downstream, thin oily film
interspersed with water was observed. Sediments were observed to be
dark with mixture of brownish sandy soil with TPH ranging from
17,800 mg/kg in Lot 1 to 88,500 mg/kg in Lot 12 samples. Brass
Canal bank soil was observed to be sandy from ground surface to 3m,
below ground surface (bgs) it was silty-sandy and brownish while
subsurface soil (4-10m bgs) was sandy-clayey and whitish/grayish
with typical hydrocarbon smell. Preliminary results obtained so far
have been very promising but were proprietary. This project is
considered, to the best of technical literature knowledge, the first
large-scale on-site bioremediation project in the Niger Delta region,
Nigeria.
Abstract: Micro-electromechanical system (MEMS)
accelerometers and gyroscopes are suitable for the inertial navigation
system (INS) of many applications due to low price, small
dimensions and light weight. The main disadvantage in a comparison
with classic sensors is a worse long term stability. The estimation
accuracy is mostly affected by the time-dependent growth of inertial
sensor errors, especially the stochastic errors. In order to eliminate
negative effects of these random errors, they must be accurately
modeled. In this paper, the Allan variance technique will be used in
modeling the stochastic errors of the inertial sensors. By performing
a simple operation on the entire length of data, a characteristic curve
is obtained whose inspection provides a systematic characterization
of various random errors contained in the inertial-sensor output data.
Abstract: Pt/γ-Al2O3 membrane catalysts were prepared via an
evaporative-crystallization deposition method. The obtained Pt/γ-
Al2O3 catalyst activity was tested after characterization (SEM-EDAX
observation, BET measurement, permeability assessment) in the
catalytic oxidation of selected volatile organic compound (VOC) i.e.
propane, fed in mixture of oxygen. The VOC conversion (nearly
90%) obtained by varying the operating temperature showed that
flow-through membrane reactor might do better in the abatement of
VOCs.
Abstract: The principle of the seismic performance evaluation methods is to provide a measure of capability for a building or set of buildings to be damaged by an earthquake. The common objective of many of these methods is to supply classification criteria. The purpose of this study is to present a method for assessing the seismic performance of structures, based on Pushover method; we are particularly interested in reinforced concrete frame structures, which represent a significant percentage of damaged structures after a seismic event. The work is based on the characterization of seismic movement of the various earthquake zones in terms of PGA and PGD that is obtained by means of SIMQK_GR and PRISM software and the correlation between the points of performance and the scalar characterizing the earthquakes will developed.
Abstract: The objective of this work is to study the effect of two
key factors - external magnetic field and applied current density
during template-based electrodeposition of nickel nanowires using an
electrode distance of 20 mm. Morphology, length, crystallite size and
crystallographic characterization of the grown nickel nanowires at an
electrode distance of 20mm are presented. For this electrode distance
of 20 mm, these two key electrodeposition factors when coupled was
found to reduce crystallite size with a higher growth length and
preferred orientation of Ni crystals. These observed changes can be
inferred to be due to coupled interaction forces induced by the
intensity of applied electric field (current density) and external
magnetic field known as magnetohydrodynamic (MHD) effect during
the electrodeposition process.
Abstract: This work is undertaken to develop a methodology to enhance the management of dredged marine and river sediments in the North of France. The main objective of this study is to determine the main characteristics of these sediments. In this order, physical, mineralogical and chemical properties of both types of sediments are measured. Moreover, their potential impacts on the environment are assessed throughout leaching tests. From the obtained results, the potential of their use in road engineering is discussed.
Abstract: An experimental study was performed to investigate
the behavior and strength of proposed technique to connect
reinforced concrete (RC) beam to steel or composite columns. This
approach can practically be used in several types of building
construction. In this technique, the main beam of the frame consists
of a transfer part (part of beam; Tr.P) and a common reinforcement
concrete beam. The transfer part of the beam is connected to the
column, whereas the rest of the beam is connected to the transfer part
from each side. Four full-scale beam-column connections were tested
under static loading. The test parameters were the length of the
transfer part and the column properties. The test results show that
using of the transfer part technique leads to modify the deformation
capabilities for the RC beam and hence it increases its resistance
against failure. Increase in length of the transfer part did not
necessarily indicate an enhanced behavior. The test results contribute
to the characterization of the connection behavior between RC beam -
steel column and can be used to calibrate numerical models for the
simulation of this type of connection.
Abstract: Catalytic combustion of methane is imperative due to
stability of methane at low temperature. Methane (CH4), therefore,
remains unconverted in vehicle exhausts thereby causing greenhouse
gas GHG emission problem. In this study, heterogeneous catalysts of
palladium with bio-char (2 wt% Pd/Bc) and Al2O3 (2wt% Pd/ Al2O3)
supports were prepared by incipient wetness impregnation and then
subsequently tested for catalytic combustion of CH4. Support-porous
heterogeneous catalytic combustion (HCC) material were selected
based on factors such as surface area, porosity, thermal stability,
thermal conductivity, reactivity with reactants or products, chemical
stability, catalytic activity, and catalyst life. Sustainable and
renewable support-material of bio-mass char derived from palm shell
waste material was compared with those from the conventional
support-porous materials. Kinetic rate of reaction was determined for
combustion of methane on Palladium (Pd) based catalyst with Al2O3
support and bio-char (Bc). Material characterization was done using
TGA, SEM, and BET surface area. The performance test was
accomplished using tubular quartz reactor with gas mixture ratio of
3% methane and 97% air. The methane porous-HCC conversion was
carried out using online gas analyzer connected to the reactor that
performed porous-HCC. BET surface area for prepared 2 wt% Pd/Bc
is smaller than prepared 2wt% Pd/ Al2O3 due to its low porosity
between particles. The order of catalyst activity based on kinetic rate
on reaction of catalysts in low temperature was 2wt%
Pd/Bc>calcined 2wt% Pd/ Al2O3> 2wt% Pd/ Al2O3>calcined 2wt%
Pd/Bc. Hence agro waste material can successfully be utilized as an
inexpensive catalyst support material for enhanced CH4 catalytic
combustion.
Abstract: Bir El Djir is an important coastal township in Oran
department, located at 450 Km far away from Algiers on northwest of
Algeria. In this coastal area, the urban sprawl is one of the main
problems that reduce the limited highly fertile land. So, using the
remote sensing and GIS technologies have shown their great
capabilities to solve many earth resources issues.
The aim of this study is to produce land use and cover map for the
studied area at varied periods to monitor possible changes that may
occurred, particularly in the urban areas and subsequently predict
likely changes. For this, two spatial images SPOT and Landsat
satellites from 1987 and 2014 respectively were used to assess the
changes of urban expansion and encroachment during this period
with photo-interpretation and GIS approach.
The results revealed that the town of Bir El Djir has shown a
highest growth rate in the period 1987-2014 which is 1201.5 hectares
in terms of area. These expansions largely concern the new real estate
constructions falling within the social and promotional housing
programs launched by the government.
The most urban expansion is characterized by the new
construction in the form of spontaneous or peripheral precarious
habitat, but also unstructured slums settled especially in the
southeastern part of town.
Abstract: The thermal behavior of a large-scale, phase change material (PCM) enhanced building envelope system was studied in regard to the need for pre-fabricated construction in subtropical regions. The proposed large-scale envelope consists of a reinforced aluminum skin, insulation core, phase change material and reinforced gypsum board. The PCM impact on an energy efficiency of an enveloped room was resolved by validation of the EnergyPlus numerical scheme and optimization of a smart material location in the core. The PCM location was optimized by a minimization method of a cooling energy demand. It has been shown that there is good agreement between the test and simulation results. The optimal location of the PCM layer in Hong Kong summer conditions has been then recomputed for core thicknesses of 40, 60 and 80 mm. A non-dimensional value of the optimal PCM location was obtained to be same for all the studied cases and the considered external and internal conditions.