Bioinformatics and Molecular Biological Characterization of a Hypothetical Protein SAV1226 as a Potential Drug Target for Methicillin/Vancomycin- Staphylococcus aureus Infections
Methicillin/multiple-resistant Staphylococcus aureus
(MRSA) are infectious bacteria that are resistant to common
antibiotics. A previous in silico study in our group has identified a
hypothetical protein SAV1226 as one of the potential drug targets. In
this study, we reported the bioinformatics characterization, as well as
cloning, expression, purification and kinetic assays of hypothetical
protein SAV1226 from methicillin/vancomycin-resistant
Staphylococcus aureus Mu50 strain. MALDI-TOF/MS analysis
revealed a low degree of structural similarity with known proteins.
Kinetic assays demonstrated that hypothetical protein SAV1226 is
neither a domain of an ATP dependent dihydroxyacetone kinase nor
of a phosphotransferase system (PTS) dihydroxyacetone kinase,
suggesting that the function of hypothetical protein SAV1226 might
be misannotated on public databases such as UniProt and
InterProScan 5.
[1] Voyich, J. et al. (2006) J. Infect. Dis., 194(12):1761-1770.
[2] CDC (2007) MRSA: Methicillin-resistant Staphylococcus aureus in
Healthcare Settings.
[3] Klevens, R. et al. (2007), J. Am. Med. Assn., 298(15):1763-1771.
[4] Mulligan, E. et al. (1993). Am. J. Med. 94: 313-328.
[5] Cunha, A. (2005), Clin. Microbiol. Infec., 11, (s4): 33–42,
[6] Walsh, T. et al. (2009). Sci. Am. 301(1): 44-51.
[7] Weinstein, R. A. (2001) Emerg Infect Dis. 7(2):188–192.
[8] Nathan, C. (2004) Nature, 431: 899–902.
[9] Tenover, F. C. (2006) Am. J. Med.119 (6 Suppl 1):S3–S10.
[10] Barrett C. et. al. (2003) Curr. Opin. Biotech. 14: 621–626.
[11] Walsh, T. (2003) Nature Rev. Microbiol. 1:65–70.
[12] Haag, N. et al. (2012), International Journal on Advances in Life
Sciences, 4 (1&2):21-32.
[13] Unpublished result.
[14] Daniel, R. et al. (1995) J. Bacteriol. 177:4392–4401
[15] Gutknecht, R. et al. (2001) EMBO J., 20(10):2480-6.
[16] http://www.phantome.org/PhageSeed/SubsysEditor.cgi?page=ShowSubs
ystem&subsystem=Dihydroxyacetone_kinases (11/26/2014)
[17] http://www.ncbi.nlm.nih.gov/protein/15924216 (11/26/2014)
[18] http://www.genome.jp/dbget-bin/www_bget?sav:SAV1226(11/26/2014)
[19] http://www.uniprot.org/uniprot/Q99UP2 (11/26/2014)
[20] http://www.ebi.ac.uk/Tools/pfa/iprscan (11/26/2014)
[21] Altschul, F. et al. (1997), Nucleic Acids Res. 25:3389-3402.8
[1] Voyich, J. et al. (2006) J. Infect. Dis., 194(12):1761-1770.
[2] CDC (2007) MRSA: Methicillin-resistant Staphylococcus aureus in
Healthcare Settings.
[3] Klevens, R. et al. (2007), J. Am. Med. Assn., 298(15):1763-1771.
[4] Mulligan, E. et al. (1993). Am. J. Med. 94: 313-328.
[5] Cunha, A. (2005), Clin. Microbiol. Infec., 11, (s4): 33–42,
[6] Walsh, T. et al. (2009). Sci. Am. 301(1): 44-51.
[7] Weinstein, R. A. (2001) Emerg Infect Dis. 7(2):188–192.
[8] Nathan, C. (2004) Nature, 431: 899–902.
[9] Tenover, F. C. (2006) Am. J. Med.119 (6 Suppl 1):S3–S10.
[10] Barrett C. et. al. (2003) Curr. Opin. Biotech. 14: 621–626.
[11] Walsh, T. (2003) Nature Rev. Microbiol. 1:65–70.
[12] Haag, N. et al. (2012), International Journal on Advances in Life
Sciences, 4 (1&2):21-32.
[13] Unpublished result.
[14] Daniel, R. et al. (1995) J. Bacteriol. 177:4392–4401
[15] Gutknecht, R. et al. (2001) EMBO J., 20(10):2480-6.
[16] http://www.phantome.org/PhageSeed/SubsysEditor.cgi?page=ShowSubs
ystem&subsystem=Dihydroxyacetone_kinases (11/26/2014)
[17] http://www.ncbi.nlm.nih.gov/protein/15924216 (11/26/2014)
[18] http://www.genome.jp/dbget-bin/www_bget?sav:SAV1226(11/26/2014)
[19] http://www.uniprot.org/uniprot/Q99UP2 (11/26/2014)
[20] http://www.ebi.ac.uk/Tools/pfa/iprscan (11/26/2014)
[21] Altschul, F. et al. (1997), Nucleic Acids Res. 25:3389-3402.8
@article{"International Journal of Biological, Life and Agricultural Sciences:70209", author = "Nichole Haag and Kimberly Velk and Tyler McCune and Chun Wu", title = "Bioinformatics and Molecular Biological Characterization of a Hypothetical Protein SAV1226 as a Potential Drug Target for Methicillin/Vancomycin- Staphylococcus aureus Infections", abstract = "Methicillin/multiple-resistant Staphylococcus aureus
(MRSA) are infectious bacteria that are resistant to common
antibiotics. A previous in silico study in our group has identified a
hypothetical protein SAV1226 as one of the potential drug targets. In
this study, we reported the bioinformatics characterization, as well as
cloning, expression, purification and kinetic assays of hypothetical
protein SAV1226 from methicillin/vancomycin-resistant
Staphylococcus aureus Mu50 strain. MALDI-TOF/MS analysis
revealed a low degree of structural similarity with known proteins.
Kinetic assays demonstrated that hypothetical protein SAV1226 is
neither a domain of an ATP dependent dihydroxyacetone kinase nor
of a phosphotransferase system (PTS) dihydroxyacetone kinase,
suggesting that the function of hypothetical protein SAV1226 might
be misannotated on public databases such as UniProt and
InterProScan 5.", keywords = "Dihydroxyacetone kinase, essential genes,
Methicillin-resistant Staphylococcus aureus, drug target.", volume = "9", number = "6", pages = "639-5", }