Investigation on Toxicity of Manufactured Nanoparticles to Bioluminescence Bacteria Vibrio fischeri

Acute toxicity of nano SiO2, ZnO, MCM-41 (Meso pore silica), Cu, Multi Wall Carbon Nano Tube (MWCNT), Single Wall Carbon Nano Tube (SWCNT) , Fe (Coated) to bacteria Vibrio fischeri using a homemade luminometer , was evaluated. The values of the nominal effective concentrations (EC), causing 20% and 50% inhibition of biouminescence, using two mathematical models at two times of 5 and 30 minutes were calculated. Luminometer was designed with Photomultiplier (PMT) detector. Luminol chemiluminescence reaction was carried out for the calibration graph. In the linear calibration range, the correlation coefficients and coefficient of Variation (CV) were 0.988 and 3.21% respectively which demonstrate the accuracy and reproducibility of the instrument that are suitable. The important part of this research depends on how to optimize the best condition for maximum bioluminescence. The culture of Vibrio fischeri with optimal conditions in liquid media, were stirring at 120 rpm at a temperature of 150C to 180C and were incubated for 24 to 72 hours while solid medium was held at 180C and for 48 hours. Suspension of nanoparticles ZnO, after 30 min contact time to bacteria Vibrio fischeri, showed the highest toxicity while SiO2 nanoparticles showed the lowest toxicity. After 5 min exposure time, the toxicity of ZnO was the strongest and MCM-41 was the weakest toxicant component.

Fatigue Life of an Anti-Roll Bar of a Passenger Vehicle

In the present paper, Fatigue life assessment of an anti-roll bar component of a passenger vehicle, is investigated by ANSYS 11 software. A stress analysis is also carried out by the finite element technique for the determination of highly stressed regions on the bar. Anti-roll bar is a suspension element used at the front, rear, or at both ends of a car that reduces body roll by resisting any unequal vertical motion between the pair of wheels to which it is connected. As a first stage, fatigue damage models proposed by some well-known references and the corresponding assumptions are discussed and some enhancements are proposed. Then, fracture analysis of an anti-roll bar of an automobile is carried out. The analysed type of the anti-roll bar is especially important as many cases are reported about the fracture after a 100,000 km of travel fatigue and fracture conditions. This paper demonstrates fatigue life of an anti-roll bar and then evaluated by experimental analytically results from other researcher.

Deflocculation and Gelation of Porcelain Ceramics

Deflocculation and gel characterization were investigated for three different composition of porcelain slips at specific gravity 1.8. The suspensions were dispersed with sodium silicate (Na2SiO3) in under-deflocculated slips and fully deflocculated slips. The rheology characterization of slips was conducted by the deflocculation curves and the gel curves. The results showed that decreasing the amount of the ball clay composition in the slips consumed less dosages of the dispersants. The under-deflocculated slips tended to have a gelation rate faster than the fully deflocculated slips.

Designing Pictogram for Food Portion Size

The objective of this paper is to investigate a new approach based on the idea of pictograms for food portion size. This approach adopts the model of the United States Pharmacopeia- Drug Information (USP-DI). The representation of each food portion size composed of three parts: frame, the connotation of dietary portion sizes and layout. To investigate users- comprehension based on this approach, two experiments were conducted, included 122 Taiwanese people, 60 male and 62 female with ages between 16 and 64 (divided into age groups of 16-30, 31-45 and 46-64). In Experiment 1, the mean correcting rate of the understanding level of food items is 48.54% (S.D.= 95.08) and the mean response time 2.89sec (S.D.=2.14). The difference on the correct rates for different age groups is significant (P*=0.00

Steady State of Passive and Active Suspensions in the Physical Domain

The steady state response of bond graphs representing passive and active suspension is presented. A bond graph with preferred derivative causality assignment to get the steady state is proposed. A general junction structure of this bond graph is proposed. The proposed methodology to passive and active suspensions is applied.

Kinematic Modeling and Workspace Analysis of a Spatial Cable Suspended Robot as Incompletely Restrained Positioning Mechanism

This article proposes modeling, simulation and kinematic and workspace analysis of a spatial cable suspended robot as incompletely Restrained Positioning Mechanism (IRPM). These types of robots have six cables equal to the number of degrees of freedom. After modeling, the kinds of workspace are defined then an statically reachable combined workspace for different geometric structures of fixed and moving platform is obtained. This workspace is defined as the situations of reference point of the moving platform (center of mass) which under external forces such as weight and with ignorance of inertial effects, the moving platform should be in static equilibrium under conditions that length of all cables must not be exceeded from the maximum value and all of cables must be at tension (they must have non-negative tension forces). Then the effect of various parameters such as the size of moving platform, the size of fixed platform, geometric configuration of robots, magnitude of applied forces and moments to moving platform on workspace of these robots with different geometric configuration are investigated. Obtained results should be effective in employing these robots under different conditions of applied wrench for increasing the workspace volume.

Numerical Analysis of Flow through Abrasive Water Suspension Jet: The Effect of Garnet, Aluminum Oxide and Silicon Carbide Abrasive on Skin Friction Coefficient Due To Wall Shear and Jet Exit Kinetic Energy

It is well known that the abrasive particles in the abrasive water suspension has significant effect on the erosion characteristics of the inside surface of the nozzle. Abrasive particles moving with the flow cause severe skin friction effect, there by altering the nozzle diameter due to wear which in turn reflects on the life of the nozzle for effective machining. Various commercial abrasives are available for abrasive water jet machining. The erosion characteristic of each abrasive is different. In consideration of this aspect, in the present work, the effect of abrasive materials namely garnet, aluminum oxide and silicon carbide on skin friction coefficient due to wall shear stress and jet kinetic energy has been analyzed. It is found that the abrasive material of lower density produces a relatively higher skin friction effect and higher jet exit kinetic energy.

Prognostic and Diagnostic Modes of Mathematical Model for the Pre-operation of Suspended Sediment Transport model in Estuaries and Coastal areas

Both prognostic and diagnostic modes of a 3D baroclinic model in hydrodynamic and sediment transport models of the Princeton Ocean Model (POM) were conducted to separate prognose and diagnose effects of different hydrodynamic factors on transport of suspended sediment discharged from the rivers to the Gulf of Thailand (GoT). Both transport modes of suspended sediment distribution in the GoT were numerically simulated. It could be concluded that the suspended sediment discharged from the rivers around the GoT. Most of sediments in estuaries and coastal areas are deposited outside the GoT under the condition of wind-driven current, and very small amount of the sediments of them are transported faraway. On the basis of wind forcing, sediments from the lower GoT to the upper GoT are mainly transported south-northwestward and also continuously moved north-southwestward. An obvious 3D characteristic of suspended sediment transport is produced in the wind-driven current residual circulation condition. In this study, the transport patterns at the third layer are generally consistent with the typhoon-induced strong currents in two case studies of Typhoon Linda 1997. The case studies presented the prognostic and diagnostic modes during 00UTC28OCT1997 to 12UTC06NOV1997 in a short period with the current condition for pre-operation of the suspended sediment transport model in estuaries and coastal areas.

Novel Design and Analysis of a Brake Rotor

Over the course of the past century, the global automotive industry-s stance towards safety has evolved from one of contempt to one nearing reverence. A suspension system that provides safe handling and cornering capabilities can, with the help of an efficient braking system, improve safety to a large extent. The aim of this research is to propose a new automotive brake rotor design and to compare it with automotive vented disk rotor. Static structural and transient thermal analysis have been carried out on the vented disk rotor and proposed rotor designs to evaluate and compare their performance. Finite element analysis was employed for both static structural and transient thermal analysis. Structural analysis was carried out to study the stress and deformation pattern of the rotors under extreme loads. Time varying temperature load was applied on the rotors and the temperature distribution was analysed considering cooling parameters (convection and radiation). This dissertation illustrates the use of Finite Element Methods to examine models, concluding with a comparative study of the proposed rotor design and the conventional vented disk rotor for structural stability and thermal efficiency.

Optimal Controller Design for Linear Magnetic Levitation Rail System

In many applications, magnetic suspension systems are required to operate over large variations in air gap. As a result, the nonlinearities inherent in most types of suspensions have a significant impact on performance. Specifically, it may be difficult to design a linear controller which gives satisfactory performance, stability, and disturbance rejection over a wide range of operating points. in this paper an optimal controller based on discontinuous mathematical model of the system for an electromagnetic suspension system which is applied in magnetic trains has been designed . Simulations show that the new controller can adapt well to the variance of suspension mass and gap, and keep its dynamic performance, thus it is superior to the classic controller.

A Comparison of Artificial Neural Networks for Prediction of Suspended Sediment Discharge in River- A Case Study in Malaysia

Prediction of highly non linear behavior of suspended sediment flow in rivers has prime importance in the field of water resources engineering. In this study the predictive performance of two Artificial Neural Networks (ANNs) namely, the Radial Basis Function (RBF) Network and the Multi Layer Feed Forward (MLFF) Network have been compared. Time series data of daily suspended sediment discharge and water discharge at Pari River was used for training and testing the networks. A number of statistical parameters i.e. root mean square error (RMSE), mean absolute error (MAE), coefficient of efficiency (CE) and coefficient of determination (R2) were used for performance evaluation of the models. Both the models produced satisfactory results and showed a good agreement between the predicted and observed data. The RBF network model provided slightly better results than the MLFF network model in predicting suspended sediment discharge.

Advanced Geolocation of IP Addresses

Tracing and locating the geographical location of users (Geolocation) is used extensively in todays Internet. Whenever we, e.g., request a page from google we are - unless there was a specific configuration made - automatically forwarded to the page with the relevant language and amongst others, dependent on our location identified, specific commercials are presented. Especially within the area of Network Security, Geolocation has a significant impact. Because of the way the Internet works, attacks can be executed from almost everywhere. Therefore, for an attribution, knowledge of the origination of an attack - and thus Geolocation - is mandatory in order to be able to trace back an attacker. In addition, Geolocation can also be used very successfully to increase the security of a network during operation (i.e. before an intrusion actually has taken place). Similar to greylisting in emails, Geolocation allows to (i) correlate attacks detected with new connections and (ii) as a consequence to classify traffic a priori as more suspicious (thus particularly allowing to inspect this traffic in more detail). Although numerous techniques for Geolocation are existing, each strategy is subject to certain restrictions. Following the ideas of Endo et al., this publication tries to overcome these shortcomings with a combined solution of different methods to allow improved and optimized Geolocation. Thus, we present our architecture for improved Geolocation, by designing a new algorithm, which combines several Geolocation techniques to increase the accuracy.

Comparison between Skyhook and Minimax Control Strategies for Semi-active Suspension System

This paper describes the development, modeling, and testing of skyhook and MiniMax control strategies of semi-active suspension. The control performances are investigated using Matlab/Simulink [1], with a two-degree-of-freedom quarter car semiactive suspension system model. The comparison and evaluation of control result are made using software-in-the-loop simulation (SILS) method. This paper also outlines the development of a hardware-inthe- loop simulation (HILS) system. The simulation results show that skyhook strategy can significantly reduce the resonant peak of body and provide improvement in vehicle ride comfort. Otherwise, MiniMax strategy can be employed to effectively improve drive safety of vehicle by influencing wheel load. The two strategies can be switched to control semi-active suspension system to fulfill different requirement of vehicle in different stages.

Effective Self-Preservation of Methane Hydrate Particles in Crude Oils

In this work we investigated the behavior of methane hydrates dispersed in crude oils from different fields at temperatures below 0°C. In case of crude oil emulsion the size of water droplets is in the range of 50e100"m. The size of hydrate particles formed from droplets is the same. The self-preservation is not expected in this field. However, the self-preservation of hydrates with the size of particles 24±18"m (electron microscopy data) in suspensions is observed. Similar results were obtained for four different kinds of crude oil and model system such as asphaltenes, resins and wax in ndecane. This result can allow developing effective methods to prevent the formation and elimination of gas-hydrate plugs in pipelines under low temperature conditions (e. g. in Eastern Siberia). There is a prospective to use experiment results for working out the technology of associated petroleum gas recovery.

Production of Spherical Ag/ZnO Nanocomposite Particles for Photocatalytic Applications

Noble metal participation in nanostructured semiconductor catalysts has drawn much interest because of their improved properties. Recently, it has been discussed by many researchers that Ag participation in TiO2, CuO, ZnO semiconductors showed improved photocatalytic and optical properties. In this research, Ag/ZnO nanocomposite particles were prepared by Ultrasonic Spray Pyrolysis(USP) Method. 0.1M silver and zinc nitrate aqueous solutions were used as precursor solutions. The Ag:Zn atomic ratio of the solution was selected 1:1. Experiments were taken place under constant air flow of 400 mL/min at 800°C furnace temperature. Particles were characterized by X-Ray Diffraction (XRD), Scanning Electron Microscope (SEM) and Energy Dispersive Spectroscopy (EDS). The crystallite sizes of Ag and ZnO in composite particles are 24.6 nm, 19.7 nm respectively. Although, spherical nanocomposite particles are in a range of 300- 800 nm, these particles are formed by the aggregation of primary particles which are in a range of 20-60 nm.

Optimization of Double Wishbone Suspension System with Variable Camber Angle by Hydraulic Mechanism

Simulation accuracy by recent dynamic vehicle simulation multidimensional expression significantly has progressed and acceptable results not only for passive vehicles but also for active vehicles normally equipped with advanced electronic components is also provided. Recently, one of the subjects that has it been considered, is increasing the safety car in design. Therefore, many efforts have been done to increase vehicle stability especially in the turn. One of the most important efforts is adjusting the camber angle in the car suspension system. Optimum control camber angle in addition to the vehicle stability is effective in the wheel adhesion on road, reducing rubber abrasion and acceleration and braking. Since the increase or decrease in the camber angle impacts on the stability of vehicles, in this paper, a car suspension system mechanism is introduced that could be adjust camber angle and the mechanism is application and also inexpensive. In order to reach this purpose, in this paper, a passive double wishbone suspension system with variable camber angle is introduced and then variable camber mechanism designed and analyzed for study the designed system performance, this mechanism is modeled in Visual Nastran software and kinematic analysis is revealed.

A Novel Plausible Deniability Scheme in Secure Steganography

The goal of steganography is to avoid drawing suspicion to the transmission of a hidden message. If suspicion is raised, steganography may fail. The success of steganography depends on the secrecy of the action. If steganography is detected, the system will fail but data security depends on the robustness of the applied algorithm. In this paper, we propose a novel plausible deniability scheme in steganography by using a diversionary message and encrypt it with a DES-based algorithm. Then, we compress the secret message and encrypt it by the receiver-s public key along with the stego key and embed both messages in a carrier using an embedding algorithm. It will be demonstrated how this method can support plausible deniability and is robust against steganalysis.

Fabrication of Microfluidic Device for Quantitative Monitoring of Algal Cell Behavior Using X-ray LIGA Technology

In this paper, a simple microfluidic device for monitoring algal cell behavior is proposed. An array of algal microwells is fabricated by PDMS soft-lithography using X-ray LIGA mold, placed on a glass substrate. Two layers of replicated PDMS and substrate are attached by oxygen plasma bonding, creating a microchannel for the microfluidic system. Algal cell are loaded into the microfluidic device, which provides positive charge on the bottom surface of wells. Algal cells, which are negative charged, can be attracted to the bottom of the wells via electrostatic interaction. By varying the concentration of algal cells in the loading suspension, it is possible to obtain wells with a single cell. Liquid medium for cells monitoring are flown continuously over the wells, providing nutrient and waste exchange between the well and the main flow. This device could lead to the uncovering of the quantitative biology of the algae, which is a key to effective and extensive algal utilizations in the field of biotechnology, food industry and bioenergy research and developments.

Application of Genetic Engineering for Chromium Removal from Industrial Wastewater

The treatment of the industrial wastewater can be particularly difficult in the presence of toxic compounds. Excessive concentration of Chromium in soluble form is toxic to a wide variety of living organisms. Biological removal of heavy metals using natural and genetically engineered microorganisms has aroused great interest because of its lower impact on the environment. Ralston metallidurans, formerly known as Alcaligenes eutrophus is a LProteobacterium colonizing industrial wastewater with a high content of heavy metals. Tris-buffered mineral salt medium was used for growing Alcaligenes eutrophus AE104 (pEBZ141). The cells were cultivated for 18 h at 30 oC in Tris-buffered mineral salt medium containing 3 mM disodium sulphate and 46 mM sodium gluconate as the carbon source. The cells were harvested by centrifugation, washed, and suspended in 10 mM Tris HCl, pH 7.0, containing 46 mM sodium gluconate, and 5 mM Chromium. Interaction among induction of chr resistance determinant, and chromate reduction have been demonstrated. Results of this study show that the above bacteria can be very useful for bioremediation of chromium from industrial wastewater.