Abstract: High precision in motion is required to manipulate the
micro objects in precision industries for micro assembly, cell
manipulation etc. Precision manipulation is achieved based on the
appropriate mechanism design of micro devices such as
microgrippers. Design of a compliant based mechanism is the better
option to achieve a highly precised and controlled motion. This
research article highlights the method of designing a compliant based
three fingered microgripper suitable for holding asymmetric objects.
Topological optimization technique, a systematic method is
implemented in this research work to arrive a topologically optimized
design of the mechanism needed to perform the required micro
motion of the gripper. Optimization technique has a drawback of
generating senseless regions such as node to node connectivity and
staircase effect at the boundaries. Hence, it is required to have post
processing of the design to make it manufacturable. To reduce the
effect of post processing stage and to preserve the edges of the image,
a cubic spline interpolation technique is introduced in the MATLAB
program. Structural performance of the topologically developed
mechanism design is tested using finite element method (FEM)
software. Further the microgripper structure is examined to find its
fatigue life and vibration characteristics.
Abstract: A numerical method is developed for simulating
the motion of particles with arbitrary shapes in an effectively
infinite or bounded viscous flow. The particle translational and
angular motions are numerically investigated using a fluid-structure
interaction (FSI) method based on the Arbitrary-Lagrangian-Eulerian
(ALE) approach and the dynamic mesh method (smoothing and
remeshing) in FLUENT ( ANSYS Inc., USA). Also, the effects of
arbitrary shapes on the dynamics are studied using the FSI method
which could be applied to the motions and deformations of a single
blood cell and multiple blood cells, and the primary thrombogenesis
caused by platelet aggregation. It is expected that, combined with a
sophisticated large-scale computational technique, the simulation
method will be useful for understanding the overall properties of blood
flow from blood cellular level (microscopic) to the resulting
rheological properties of blood as a mass (macroscopic).
Abstract: Adhesion to the human intestinal cell is considered
as one of the main selection criteria of lactic acid bacteria for
probiotic use. The adhesion ability of two Bifidobacteriums strains
Bifidobacterium longum BB536 and Bifidobacterium
psudocatenulatum G4 was done using HT-29 human epithelium
cell line as in vitro study. Four different level of pH were used 5.6,
5.7, 6.6, and 6.8 with four different times 15, 30, 60, and 120 min.
Adhesion was quantified by counting the adhering bacteria after
Gram staining. The adhesion of B. longum BB536 was higher than
B. psudocatenulatum G4. Both species showed significant
different in the adhesion properties at the factors tested. The
highest adhesion for both Bifidobacterium was observed at 120
min and the low adhesion was in 15 min. The findings of this
study will contribute to the introduction of new effective probiotic
strain for future utilization.
Abstract: Deaths from cardiovascular diseases have decreased substantially over the past two decades, largely as a result of advances in acute care and cardiac surgery. These developments have produced a growing population of patients who have survived a myocardial infarction. These patients need to be continuously monitored so that the initiation of treatment can be given within the crucial golden hour. The available conventional methods of monitoring mostly perform offline analysis and restrict the mobility of these patients within a hospital or room. Hence the aim of this paper is to design a Portable Cardiac Telemedicine System to aid the patients to regain their independence and return to an active work schedule, there by improving the psychological well being. The portable telemedicine system consists of a Wearable ECG Transmitter (WET) and a slightly modified mobile phone, which has an inbuilt ECG analyzer. The WET is placed on the body of the patient that continuously acquires the ECG signals from the high-risk cardiac patients who can move around anywhere. This WET transmits the ECG to the patient-s Bluetooth enabled mobile phone using blue tooth technology. The ECG analyzer inbuilt in the mobile phone continuously analyzes the heartbeats derived from the received ECG signals. In case of any panic condition, the mobile phone alerts the patients care taker by an SMS and initiates the transmission of a sample ECG signal to the doctor, via the mobile network.
Abstract: Ethanol is generally used as a therapeutic reagent against Hepatocellular carcinoma (HCC or hepatoma) worldwide, as it can induce Hepatocellular carcinoma cell apoptosis at low concentration through a multifactorial process regulated by several unknown proteins. This paper provides a simple and available proteomic strategy for exploring differentially expressed proteins in the apoptotic pathway. The appropriate concentrations of ethanol required to induce HepG2 cell apoptosis were first assessed by MTT assay, Gisma and fluorescence staining. Next, the central proteins involved in the apoptosis pathway processs were determined using 2D-PAGE, SDS-PAGE, and bio-software analysis. Finally the downregulation of two proteins, AFP and survivin, were determined by immunocytochemistry and reverse transcriptase PCR (RT-PCR) technology. The simple, useful method demonstrated here provides a new approach to proteomic analysis in key bio-regulating process including proliferation, differentiation, apoptosis, immunity and metastasis.
Abstract: There are three distinct stages in the evolution of
economic thought, namely:
1. in the first stage, the major concern was to accelerate
economic growth with increased availability of material
goods, especially in developing economies with very low
living standards, because poverty eradication meant faster
economic growth.
2. in the second stage, economists made distinction between
growth and development. Development was seen as going
beyond economic growth, and bringing certain changes in
the structure of the economy with more equitable
distribution of the benefits of growth, with the growth
coming automatic and sustained.
3. the third stage is now reached. Our concern is now with
“sustainable development", that is, development not only
for the present but also of the future.
Thus the focus changed from “sustained growth" to “sustained
development". Sustained development brings to the fore the long
term relationship between the ecology and economic development.
Since the creation of UNEP in 1972 it has worked for
development without destruction for environmentally sound and
sustained development. It was realised that the environment cannot
be viewed in a vaccum, it is not separate from development, nor is it
competing. It suggested for the integration of the environment with
development whereby ecological factors enter development planning,
socio-economic policies, cost-benefit analysis, trade, technology
transfer, waste management, educational and other specific areas.
Industrialisation has contributed to the growth of economy of
several countries. It has improved the standards of living of its people
and provided benefits to the society. It has also created in the process
great environmental problems like climate change, forest destruction
and denudation, soil erosion and desertification etc.
On the other hand, industry has provided jobs and improved the
prospects of wealth for the industrialists. The working class
communities had to simply put up with the high levels of pollution in
order to keep up their jobs and also to save their income.
There are many roots of the environmental problem. They may be
political, economic, cultural and technological conditions of the
modern society. The experts concede that industrial growth lies
somewhere close to the heart of the matter. Therefore, the objective
of this paper is not to document all roots of an environmental crisis
but rather to discuss the effects of industrial growth and
development.
We have come to the conclusion that although public intervention
is often unnecessary to ensure that perfectly competitive markets will
function in society-s best interests, such intervention is necessary
when firms or consumers pollute.
Abstract: Mobile IP has been developed to provide the
continuous information network access to mobile users. In IP-based
mobile networks, location management is an important component of
mobility management. This management enables the system to track
the location of mobile node between consecutive communications. It
includes two important tasks- location update and call delivery.
Location update is associated with signaling load. Frequent updates
lead to degradation in the overall performance of the network and the
underutilization of the resources. It is, therefore, required to devise
the mechanism to minimize the update rate. Mobile IPv6 (MIPv6)
and Hierarchical MIPv6 (HMIPv6) have been the potential
candidates for deployments in mobile IP networks for mobility
management. HMIPv6 through studies has been shown with better
performance as compared to MIPv6. It reduces the signaling
overhead traffic by making registration process local. In this paper,
we present performance analysis of MIPv6 and HMIPv6 using an
analytical model. Location update cost function is formulated based
on fluid flow mobility model. The impact of cell residence time, cell
residence probability and user-s mobility is investigated. Numerical
results are obtained and presented in graphical form. It is shown that
HMIPv6 outperforms MIPv6 for high mobility users only and for low
mobility users; performance of both the schemes is almost equivalent
to each other.
Abstract: Champs Bourcin black grape originated from
Aquitaine, France and planted in Sapa, Lao cai provice, exhibited
high total acidity (11.72 g/L). After 9 days of alcoholic fermentation
at 25oC using Saccharomyces cerevisiae UP3OY5 strain, the ethanol
concentration of wine was 11.5% v/v, however the sharp sour taste of
wine has been found. The malolactic fermentation (MLF) was carried
out by Oenococcus oeni ATCCBAA-1163 strain which had been preadapted
to acid (pH 3-4) and ethanol (8-12%v/v) conditions. We
obtained the highest vivability (83.2%) upon malolactic fermentation
after 5 days at 22oC with early stationary phase O. oeni cells preadapted
to pH 3.5 and 8% v/v ethanol in MRS medium. The malic
acid content in wine was decreased from 5.82 g/L to 0.02 g/L after
MLF (21 days at 22oC). The sensory quality of wine was
significantly improved.
Abstract: The aim of this in vitro study was to evaluate the possible interference of a Nectandra membranacea extract (i) on the labeling of blood cells (BC), (ii) on the labeling process of BC and plasma (P) proteins with technetium-99m (Tc-99m) and (iii) on the morphology of red blood cells (RBC). Blood samples were incubated with a Nectandra membranacea crude extract, stannous chloride, Tc- 99m (sodium pertechnetate) was added, and soluble (SF) and insoluble (IF) fractions were isolated. Morphometry studies were performed with blood samples incubated with Nectandra membranacea extract. The results show that the Nectandra membranacea extract does not promote significant alteration of the labeling of BC, IF-P and IF-BC. The Nectandra membranacea extract was able to alter the erythrocyte membrane morphology, but these morphological changes were not capable to interfere on the labeling of blood constituents with Tc-99m.
Abstract: Bovine viral diarrhea virus (BVDV) can cause lifelong
persistent infection. One reason for the phenomena is attributed to
BVDV infection to placenta tissue. However the mechanisms that
BVDV invades into placenta tissue remain unclear. To clarify the
molecular mechanisms, we investigated the possible means that
BVDV entered into bovine trophoblast cells (TPC). Yeast two-hybrid
system was used to identify proteins extracted from TPC, which
interact with BVDV envelope glycoprotein E2. A PGbkt7-E2 yeast
expression vector and TPC cDNA library were constructed. Through
two rounds of screening, three positive clones were identified.
Sequencing analysis indicated that all the three positive clones
encoded the same protein clathrin. Physical interaction between
clathrin and BVDV E2 protein was further confirmed by
coimmunoprecipitation experiments. This result suggested that the
clathrin might play a critical role in the process of BVDV entry into
placenta tissue and might be a novel antiviral target for preventing
BVDV infection.
Abstract: The performance of adaptive beamforming degrades
substantially in the presence of steering vector mismatches. This
degradation is especially severe in the near-field, for the
3-dimensional source location is more difficult to estimate than the
2-dimensional direction of arrival in far-field cases. As a solution, a
novel approach of near-field robust adaptive beamforming (RABF) is
proposed in this paper. It is a natural extension of the traditional
far-field RABF and belongs to the class of diagonal loading
approaches, with the loading level determined based on worst-case
performance optimization. However, different from the methods
solving the optimal loading by iteration, it suggests here a simple
closed-form solution after some approximations, and consequently,
the optimal weight vector can be expressed in a closed form. Besides
simplicity and low computational cost, the proposed approach reveals
how different factors affect the optimal loading as well as the weight
vector. Its excellent performance in the near-field is confirmed via a
number of numerical examples.
Abstract: Enzymatic saccharification of biomass for reducing
sugar production is one of the crucial processes in biofuel production
through biochemical conversion. In this study, enzymatic
saccharification of dilute potassium hydroxide (KOH) pre-treated
Tetraselmis suecica biomass was carried out by using cellulase
enzyme obtained from Trichoderma longibrachiatum. Initially, the
pre-treatment conditions were optimised by changing alkali reagent
concentration, retention time for reaction, and temperature. The T.
suecica biomass after pre-treatment was also characterized using
Fourier Transform Infrared Spectra and Scanning Electron
Microscope. These analyses revealed that the functional group such
as acetyl and hydroxyl groups, structure and surface of T. suecica
biomass were changed through pre-treatment, which is favourable for
enzymatic saccharification process. Comparison of enzymatic
saccharification of untreated and pre-treated microalgal biomass
indicated that higher level of reducing sugar can be obtained from
pre-treated T. suecica. Enzymatic saccharification of pre-treated T.
suecica biomass was optimised by changing temperature, pH, and
enzyme concentration to solid ratio ([E]/[S]). Highest conversion of
carbohydrate into reducing sugar of 95% amounted to reducing sugar
yield of 20 (wt%) from pre-treated T. suecica was obtained from
saccharification, at temperature: 40°C, pH: 4.5 and [E]/[S] of 0.1
after 72 h of incubation. Hydrolysate obtained from enzymatic
saccharification of pretreated T. suecica biomass was further
fermented into biobutanol using Clostridium saccharoperbutyliticum
as biocatalyst. The results from this study demonstrate a positive
prospect of application of dilute alkaline pre-treatment to enhance
enzymatic saccharification and biobutanol production from
microalgal biomass.
Abstract: This purpose of this paper is to develop and validate a
model to accurately predict the cell temperature of a PV module that
adapts to various mounting configurations, mounting locations, and
climates while only requiring readily available data from the module
manufacturer. Results from this model are also compared to results
from published cell temperature models. The models were used to
predict real-time performance from a PV water pumping systems in
the desert of Medenine, south of Tunisia using 60-min intervals of
measured performance data during one complete year. Statistical
analysis of the predicted results and measured data highlight possible
sources of errors and the limitations and/or adequacy of existing
models, to describe the temperature and efficiency of PV-cells and
consequently, the accuracy of performance of PV water pumping
systems prediction models.
Abstract: The present study was designed to investigate the
cardio protective role of chronic oral administration of alcoholic
extract of Terminalia arjuna in in-vivo ischemic reperfusion injury
and the induction of HSP72. Rabbits, divided into three groups, and
were administered with the alcoholic extract of the bark powder of
Terminalia arjuna (TAAE) by oral gavage [6.75mg/kg: (T1) and
9.75mg/kg: (T2), 6 days /week for 12 weeks]. In open-chest
Ketamine pentobarbitone anaesthetized rabbits, the left anterior
descending coronary artery was occluded for 15 min of ischemia
followed by 60 min of reperfusion. In the vehicle-treated group,
ischemic-reperfusion injury (IRI) was evidenced by depression of
global hemodynamic function (MAP, HR, LVEDP, peak LV (+) & (-
) (dP/dt) along with depletion of HEP compounds. Oxidative stress
in IRI was evidenced by, raised levels of myocardial TBARS and
depletion of endogenous myocardial antioxidants GSH, SOD and
catalase. Western blot analysis showed a single band corresponding
to 72 kDa in homogenates of hearts from rabbits treated with both the
doses. In the alcoholic extract of the bark powder of Terminalia
arjuna treatment groups, both the doses had better recovery of
myocardial hemodynamic function, with significant reduction in
TBARS, and rise in SOD, GSH, catalase were observed. The results
of the present study suggest that the alcoholic extract of the bark
powder of Terminalia arjuna in rabbit induces myocardial HSP 72
and augments myocardial endogenous antioxidants, without causing
any cellular injury and offered better cardioprotection against
oxidative stress associated with myocardial IR injury.
Abstract: In this paper we describe a computer-aided diagnosis (CAD) system for automated detection of pulmonary nodules in computed-tomography (CT) images. After extracting the pulmonary parenchyma using a combination of image processing techniques, a region growing method is applied to detect nodules based on 3D geometric features. We applied the CAD system to CT scans collected in a screening program for lung cancer detection. Each scan consists of a sequence of about 300 slices stored in DICOM (Digital Imaging and Communications in Medicine) format. All malignant nodules were detected and a low false-positive detection rate was achieved.
Abstract: The biomarker for colorectal cancer (CRC) is CEACAM-6 antigen (C6AG). Therefore, this study aims to develop a novel, simple and low-cost CEACAM-6 antigen immumosensor (C6AG-IMS), based on electrical impedance measurement, for precise determination of C6AG. A low-cost screen-printed graphite electrode was constructed and used as the sensor, with CEACAM-6 antibody (C6AB) immobilized on it. The procedures of sensor fabrication and antibody immobilization are simple and low-cost. Measurement of the electrical impedance at a definite frequency ranges (0.43 – 1.26 MHz) showed that the C6AG-IMS has an excellent linear (r2>0.9) response range (8.125 – 65 pg/mL), covering the normal physiological and pathological ranges of blood C6AG levels. Also, the C6AG-IMS has excellent reliability and validity, with the intraclass correlation coefficient being 0.97. In conclusion, a novel, simple, low-cost and reliable C6AG-IMS was designed and developed, being able to accurately determine blood C6AG levels in the range of pathological and normal physiological regions. The C6AG-IMS can provide a point-of-care and immediate screening results to the user at home.
Abstract: Flows over a harmonically oscillating NACA 0012
airfoil are simulated here using a two-dimensional, unsteady,
incompressibleNavier-Stokes solver.Both pure-plunging and
pitching-plunging combined oscillations are considered at a Reynolds
number of 5000. Special attention is paid to the vortex shedding and
interaction mechanism of the motions. For all the simulations
presented here, the reduced frequency (k) is fixed at a value of 2.5
and plunging amplitude (h) is selected to be in the range of 0.2-0.5.
The simulation results show that the interaction mechanism between
the leading and trailing edge vortices has a decisive effect on the
values of the resulting thrust and propulsive efficiency.
Abstract: Empty Fruit Bunches (EFB) and Palm Oil Mill
Effluent (POME) are two main wastes from oil palm industries which
contain rich lignocellulose. Degradation of EFB and POME by
microorganisms will produce hydrolytic enzyme which will degrade
cellulose and hemicellulose during composting process. However,
normal composting takes about four to six months to reach maturity.
Hence, application of fungi into compost can shorten the period of
composting. This study identifies the effect of xylanase and cellulase
produced by Aspergillus niger and Trichoderma virens on
composting process using EFB and POME. The degradation of EFB
and POME indicates the lignocellulolytic capacity of Aspergillus
niger and Trichoderma virens with more than 7% decrease in
hemicellulose and more than 25% decrease in cellulose for both
inoculated compost. Inoculation of Aspergillus niger and
Trichoderma virens also increased the enzyme activities during the
composting period compared to the control compost by 21% for both
xylanase and cellulase. Rapid rise in the activities of cellulase and
xylanase was observed by Aspergillus niger with the highest
activities of 14.41 FPU/mg and 3.89 IU/mg, respectively. Increased
activities of cellulase and xylanase also occurred in inoculation of
Trichoderma virens with the highest activities obtained at 13.21
FPU/mg and 4.43 IU/mg, respectively. Therefore, it is evident that
the inoculation of fungi can increase the enzyme activities hence
effectively degrading the EFB and POME.
Abstract: Pretreatment of lignocellulosic biomass materials from
poplar, acacia, oak, and fir with different ionic liquids (ILs)
containing 1-alkyl-3-methyl-imidazolium cations and various anions
has been carried out. The dissolved cellulose from biomass was
precipitated by adding anti-solvents into the solution and vigorous
stirring. Commercial cellulases Celluclast 1.5L and Accelerase 1000
have been used for hydrolysis of untreated and pretreated
lignocellulosic biomass. Among the tested ILs, [Emim]COOCH3
showed the best efficiency, resulting in highest amount of liberated
reducing sugars. Pretreatment of lignocellulosic biomass using
glycerol-ionic liquids combined pretreatment and dilute acid-ionic
liquids combined pretreatment were evaluated and compared with
glycerol pretreatment, ionic liquids pretreatment and dilute acid
pretreatment.
Abstract: Nowadays it is a trend for electronic circuit designers to
integrate all system components on a single-chip. This paper proposed
the design of a single-chip proportional to absolute temperature
(PTAT) sensor including a voltage reference circuit using CEDEC
0.18m CMOS Technology. It is a challenge to design asingle-chip
wide range linear response temperature sensor for many applications.
The channel widths between the compensation transistor and the
reference transistor are critical to design the PTAT temperature sensor
circuit. The designed temperature sensor shows excellent linearity
between -100°C to 200° and the sensitivity is about 0.05mV/°C.
The chip is designed to operate with a single voltage source of 1.6V.