Abstract: Quercetin and (+)-catechin are metabolites present in Phyllanthus niruri plant, have potential in medicinal uses as anticancer and antioxidant agents. Studies on production of quercetin and (+)-catechin from P. niruri callus culture via in vitro technique were carried out and the results were compared to the intact plant. P. niruri explants were cultured on Murashige and Skoog (MS) solidified media supplemented with several phytohormone combinations for one month. The metabolites were extracted from P. niruri callus and intact plant by using carbon dioxide supercritical fluid extraction (SFE) with ethanol as modifier and solvent extraction techniques. The extracts were analyzed by means of HPLC method. Results showed that P. niruri callus culture was successfully established. The highest content of quercetin (1.72%) was found from P. niruri callus grown in media supplemented with 0.8mg/L kinetin and 0.2mg/L 2,4-dicholophenoxyacetic acid (2,4-D), which was 1.2 fold higher than intact plant. Meanwhile, the highest amounts of (+)-catechin (0.63%) was found from P. niruri callus grown in media with addition of 0.2mg/L 1-naphthalene acetic acid (NAA) and 0.8mg/L 2,4-D. The SFE condition in this study showed better extraction efficiency when higher contents of selected metabolites were found in all SFE extracts compared to the common solvent extracts.
Abstract: Research on the boron (B) toxicity problems had recently considerable relation, especially in the dry regions of the world. Development of resistant varieties to B toxicity is a high priority on these regions, where the soils have high levels of B. Thus, this study aimed to assessment the resistance of wheat genotypes to B toxicity using the agronomic and physiologic parameters. For this aim, a pot experiment, based on a completely randomized design with three replications, was conducted using the soil of calcareous usthochrepts. In the study, twenty different wheat genotypes of T. aestivum and T. Durum were used. Boron fertilizer at the levels of 0 (-B), 30 mg B kg-1 (+B) as H3BO3 was applied to the pots. After harvest, plant dry matter yield was recorded, and total B concentrations in tops of wheat plants were determined. The results have revealed the existence of a large genotypic variation among wheat genotypes to their physiologic and agronomic susceptibility to B toxicity.
Abstract: Gastric ulceration is a discontinuity in gastric mucosa, usually occurs due to imbalance between the gastric mucosal protective factors, that is called gastric mucosal barrier, and the aggressive factors, to which the mucosa is exposed. This study was carried out on sixty male Sprague-Dowely rats (12- 16 weeks old) allocated into two groups. The first control group and the second Gastric lesion group which induced by oral administration of a single daily dose of aspirin at a dose of 300 mg/kg body weight for 7 consecutive-days (6% aspirin solution will be prepared and each rat will be given 5 ml of that solution/kg body weight). Blood is collected 1, 2 and 3 weeks after induction of gastric ulceration. Significant increase in serum copper, nitric oxide, and prostaglandin E2 all over the period of experiment. Significant decrease in erythrocyte superoxide dismutase (t-SOD) activities, serum (calcium, phosphorus, glucose and insulin) levels. Non-significant changes in serum sodium and potassium levels are obtained.
Abstract: Pre-germinated parboiled brown rice or Khao hang (in Thai) is paddy which undergoing the processes of soaking, steaming, drying and dehusking to obtain the edible form for consumption. The objectives of this research were to study the kinetic of pre-germinated parboiled brown rice drying using fluidization technique and to study the properties of pre-germinated parboiled brown rice after drying. The dryings were performed at the different temperatures of 110, 120 and 130 oC at the bed depth of 2 cm with the air velocity of 1.98 m/s. The results found that the higher drying temperature led to the faster moisture reduction. After drying until the moisture content of pre-germinated parboiled brown rice was lower than 14%wet basis, samples were taken to determine various qualities such as percentage of head rice and L* a* b* color values. The shade drying was used as a control. The results found that the higher drying temperature resulted in the decrease of head rice percentage. For the color assessment, the trend of L* and a* values was increased with the drying temperature, while the b* value was not significantly difference (p › 0.05) by drying temperatures. However, the b value of drying by fluidized bed dryer was higher than the control.
Abstract: The dynamic speckle or biospeckle is an interference
phenomenon generated at the reflection of a coherent light by an
active surface or even by a particulate or living body surface. The
above mentioned phenomenon gave scientific support to a method
named biospeckle which has been employed to study seed viability,
biological activity, tissue senescence, tissue water content, fruit
bruising, etc. Since the above mentioned method is not invasive and
yields numerical values, it can be considered for possible automation
associated to several processes, including selection and sorting.
Based on these preliminary considerations, this research work
proposed to study the interaction of a laser beam with vegetative
samples by measuring the incident light intensity and the transmitted
light beam intensity at several vegetative slabs of varying thickness.
Tests were carried on fifteen slices of apple tissue divided into three
thickness groups, i.e., 4 mm, 5 mm, 18 mm and 22 mm. A diode laser
beam of 10mW and 632 nm wavelength and a Samsung digital
camera were employed to carry the tests. Outgoing images were
analyzed by comparing the gray gradient of a fixed image column of
each image to obtain a laser penetration scale into the tissue,
according to the slice thickness.
Abstract: This research work proposed a study of fruit bruise detection by means of a biospeckle method, selecting the papaya fruit (Carica papaya) as testing body. Papaya is recognized as a fruit of outstanding nutritional qualities, showing high vitamin A content, calcium, carbohydrates, exhibiting high popularity all over the world, considering consumption and acceptability. The commercialization of papaya faces special problems which are associated to bruise generation during harvesting, packing and transportation. Papaya is classified as climacteric fruit, permitting to be harvested before the maturation is completed. However, by one side bruise generation is partially controlled once the fruit flesh exhibits high mechanical firmness. By the other side, mechanical loads can set a future bruise at that maturation stage, when it can not be detected yet by conventional methods. Mechanical damages of fruit skin leave an entrance door to microorganisms and pathogens, which will cause severe losses of quality attributes. Traditional techniques of fruit quality inspection include total soluble solids determination, mechanical firmness tests, visual inspections, which would hardly meet required conditions for a fully automated process. However, the pertinent literature reveals a new method named biospeckle which is based on the laser reflectance and interference phenomenon. The laser biospeckle or dynamic speckle is quantified by means of the Moment of Inertia, named after its mechanical counterpart due to similarity between the defining formulae. Biospeckle techniques are able to quantify biological activities of living tissues, which has been applied to seed viability analysis, vegetable senescence and similar topics. Since the biospeckle techniques can monitor tissue physiology, it could also detect changes in the fruit caused by mechanical damages. The proposed technique holds non invasive character, being able to generate numerical results consistent with an adequate automation. The experimental tests associated to this research work included the selection of papaya fruit at different maturation stages which were submitted to artificial mechanical bruising tests. Damages were visually compared with the frequency maps yielded by the biospeckle technique. Results were considered in close agreement.
Abstract: Staphylococcus aureus, one of the microflora in a
human external auditory canal (EAC) is frequently exposed to highfrequency
electromagnetic field (HF-EMF) generated by mobile
phones. It is normally non-pathogenic but in certain circumstances, it
can cause infections. This study investigates the changes in the
physiology of S. aureus when exposed to HF-EMF of a mobile
phone. Exponentially grown S. aureus were exposed to two
conditions of EMF irradiation (standby-mode and on-call mode) at
four durations; 15, 30, 45 and 60 min. Changes in the viability and
biofilm production of the S. aureus were compared between the two
conditions of exposure. EMF from the standby-mode has enhanced
the growth of S. aureus but during on-call, the growth was
suppressed. No significant difference in the amount of biofilm
produced in both modes of exposure was observed. Thus, HF-EMF
of mobile phone affects the viability of S. aureus but not its ability to
produce biofilm.
Abstract: In the meat processing industry the substitution of
meat with non-meat ingredients is considered an important strategy
for reducing overall production costs. The main purpose of the
current research was to evaluate differences in physical-chemical
composition of cooked sausage with different legumes additions.
Peas (Pisum sativum), beans (Phaseolus vulgaris) and lentil (Lens
culinaris) were used in preparation of sausages. The legumes at
proportion of 20% of the total weight of meat were added in
sausages. The whole ingredients were mixed, filled into casing,
compressed, cooked and cooled. After storage the samples were
sensory evaluated. The sensory evaluation was carried out using the
nine point hedonic scale and line scale. Sausages without legumes
flour was used as control sample. The main conclusion of the current
research the legumes flour can be successfully used for cooked
sausages production.
Abstract: This research studied the hypoglycemic effect of
water soluble polysaccharide (WSP) extracted from yam (Dioscorea
hispida) tuber by three different methods: aqueous extraction, papain
assisted extraction, and tempeh inoculums assisted extraction. The
two later extraction methods were aimed to remove WSP binding
protein to have more pure WSP. The hypoglycemic activities were
evaluated by means in vivo test on alloxan induced hyperglycemic
rats, glucose response test (GRT), in situ glucose absorption test
using everted sac, and short chain fatty acids (SCFAs) analysis. All
yam WSP extracts exhibited ability to decrease blood glucose level in
hyperglycemia condition as well as inhibited glucose absorption and
SCFA formation. The order of hypoglycemic activity was tempeh
inoculums assisted- >papain assisted- >aqueous WSP extracts. GRT
and in situ glucose absorption test showed that order of inhibition
was papain assisted- >tempeh inoculums assisted- >aqueous WSP
extracts. Digesta of caecum of yam WSP extracts oral fed rats had
more SCFA than control. Tempeh inoculums assisted WSP extract
exhibited the most significant hypoglycemic activity.
Abstract: In Indonesia, goat milk is often consumed and
believed as anti-allergy. The objective of this research was to study
the effect of goat milk and their fractions (casein and whey)
supplementation on total serum IgE concentrations and leukocytes
count in rat sensitized with contact allergen dinitrochlorobenzene
(DNCB). Female Wistar rats 6-8 weeks old were divided into four
groups: 1) whey, 2) casein, 3) whole milk supplementation and 4)
phosphate-buffered saline/PBS (control). The results showed that
supplementation of goat milk on rats did not affects on total serum
IgE concentrations and number of leukocytes. After sensitized with
DNCB, the monocyte percentage in rats was higher (P
Abstract: Vernonia divergens Benth., commonly known as
“Insulin Plant” (Fam: Asteraceae) is a potent sugar killer. Locally the
leaves of the plant, boiled in water are successfully administered to a
large number of diabetic patients. The present study evaluates the
putative anti-diabetic ingredients, isolated from the in vivo and in
vitro grown plantlets of V. divergens for their antimicrobial and
anticancer activities. Sterilized explants of nodal segments were
cultured on MS (Musashige and Skoog, 1962) medium in presence of
different combinations of hormones. Multiple shoots along with
bunch of roots were regenerated at 1mg l-1 BAP and 0.5 mg l-1 NAA.
Micro-plantlets were separated and sub-cultured on the double
strength (2X) of the above combination of hormones leading to
increased length of roots and shoots. These plantlets were
successfully transferred to soil and survived well in nature. The
ethanol extract of plantlets from both in vivo & in vitro sources were
prepared in soxhlet extractor and then concentrated to dryness under
reduced pressure in rotary evaporator. Thus obtainedconcentrated
extracts showed significant inhibitory activity against gram
negative bacteria like Escherichia coli and Pseudomonas
aeruginosa but no inhibition was found against gram positive
bacteria. Further, these ethanol extracts were screened for in vitro
percentage cytotoxicity at different time periods (24 h, 48 h and 72 h)
of different dilutions. The in vivo plant extract inhibited the growth of
EAC mouse cell lines in the range of 65, 66, 78, and 88% at 100, 50,
25 & 12.5μg mL-1 but at 72 h of treatment. In case of the extract of in
vitro origin, the inhibition was found against EAC cell lines even at
48h. During spectrophotometric scanning, the extracts exhibited
different maxima (ʎ) - four peaks in in vitro extracts as against single
in in vivo preparation suggesting the possible change in the nature of
ingredients during micropropagation through tissue culture
techniques.
Abstract: The Kumamoto area, Kyushu, Japan has 1,041km2 in
area and about 1milion in population. This area is a greatest area in Japan which depends on groundwater for all of drinking water. Quantity of this local groundwater use is about 200MCM during the
year. It is understood that the main recharging area of groundwater exist in the rice field zone which have high infiltrate height ahead of
100mm/ day of the irrigated water located in the middle area of the Shira-River Basin. However, by decrease of the paddy-rice planting
area by urbanization and an acreage reduction policy, the groundwater income and expenditure turned worse. Then Kumamoto city and four
companies expended financial support to increase recharging water to
underground by ponded water in the field from 2004.
In this paper, the author reported the situation of recovery of groundwater by recharge and estimates the efficiency of recharge by
statistical method.
Abstract: Herein, we report the different types of surface morphology due to the interaction between the pure protein Insulin (INS) and catanionic surfactant mixture of Sodium Dodecyl Sulfate (SDS) and Cetyl Trimethyl Ammonium Bromide (CTAB) at air/water interface obtained by the Langmuir-Blodgett (LB) technique. We characterized the aggregations by Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR) in LB films. We found that the INS adsorption increased in presence of catanionic surfactant at air/water interface. The presence of small amount of surfactant induces two-stage growth kinetics due to the pure protein absorption and protein-catanionic surface micelle interaction. The protein remains in native state in presence of small amount of surfactant mixture. Smaller amount of surfactant mixture with INS is producing surface micelle type structure. This may be considered for drug delivery system. On the other hand, INS becomes unfolded and fibrillated in presence of higher amount of surfactant mixture. In both the cases, the protein was successfully immobilized on a glass substrate by the LB technique. These results may find applications in the fundamental science of the physical chemistry of surfactant systems, as well as in the preparation of drug-delivery system.
Abstract: Selection of maize (Zea mays) hybrids with wide adaptability across diverse farming environments is important, prior to recommending them to achieve a high rate of hybrid adoption. Grain yield of 14 maize hybrids, tested in a randomized completeblock design with four replicates across 22 environments in Iran, was analyzed using site regression (SREG) stability model. The biplot technique facilitates a visual evaluation of superior genotypes, which is useful for cultivar recommendation and mega-environment identification. The objectives of this study were (i) identification of suitable hybrids with both high mean performance and high stability (ii) to determine mega-environments for maize production in Iran. Biplot analysis identifies two mega-environments in this study. The first mega-environments included KRM, KSH, MGN, DZF A, KRJ, DRB, DZF B, SHZ B, and KHM, where G10 hybrid was the best performing hybrid. The second mega-environment included ESF B, ESF A, and SHZ A, where G4 hybrid was the best hybrid. According to the ideal-hybrid biplot, G10 hybrid was better than all other hybrids, followed by the G1 and G3 hybrids. These hybrids were identified as best hybrids that have high grain yield and high yield stability. GGE biplot analysis provided a framework for identifying the target testing locations that discriminates genotypes that are high yielding and stable.
Abstract: The aim of this study was to investigate the effects of
supplementing the diluent of roosters' semen with different levels of
olive oil on motility, viability, morphology and acrosome integrity of
chicken spermatozoa after in vitro storage for up to 72 h. Semen was
collected from 60 White Layer males (62 wk of age) kept in
separated floor pens and randomly divided into six treatment groups
(10 males in each group). Experimental groups were as follows: T1
:fresh semen, T2 : semen extended 1:1 with Al – Daraji 2 diluent
(AD2D) alone, T3 – T6 :semen samples extended 1:1 with AD2D
supplemented with 2 ml, 4 ml, 6 ml or 8 ml of olive oil / 100 ml of
diluent, respectively. Semen samples were then stored at 5 °C for 24
h, 48 h or 72 h. There was a clear influence of diluent
supplementation with olive oil on the spermatozoa motility profile;
olive oil groups (T3, T4, T5 and T6) recorded the highest scores of
mass activity and individual motility during all storage periods
compared to T1 and T2 groups. In addition, the inclusion of olive oil
into semen diluent (T3, T4, T5 and T6) gave significantly higher
percentages of viable spermatozoa, normal morphologically
spermatozoa and intact acrosomes irrespective of storage period.
These results clearly show that supplementation the diluent of
roosters' semen with olive oil can improve semen quality when
semen samples in vitro stored at 5 °C for up to 72 h.
Abstract: The peel of dragon fruit is a byproduct left over after consuming. Normally, the use of plants as antioxidant source must be dried before further process. Therefore, the aim of this study is interesting to dry the peel by heat pump dryer (45 ºC) and fluidized bed dryer (110 º C) compared with the sun drying method. The sample with initial moisture content of about 85-91% wet basis was dried down to about 10% wet basis where it took 620 and 25 min for heat pump dryer and fluidized bed dryer, respectively. However, the sun drying took about 900 min to dry the peel. After that, sample was evaluated antioxidant activity, -carotene and betalains contents. The results found that the antioxidant activity and betalains contents of dried peel obtained from heat pump and fluidized bed dryings were significantly higher than that sun drying (p 0.05). Moreover, the drying by heat pump provided the highest -carotene content.
Abstract: Various assisted reproductive techniques have been
developed and refined to obtain a large number of offspring from
genetically superior animals or obtain offspring from infertile (or
subfertile) animals. The embryo transfer is one assisted reproductive
technique developed well, aimed at increased productivity of selected
females, disease control, importation and exportation of livestock,
rapid screening of AI sires for genetically recessive characteristics,
treatment or circumvention of certain types of infertility. Embryo
transfer also is a useful research tool for evaluating fetal and maternal
interactions. This technique has been applied to nearly every species
of domestic animal and many species of wildlife and exotic animals,
including humans and non-human primates. The successful of
embryo transfers have been limited to within-animal, homologous
replacement of the embryos. There are several examples of
interspecific and intergeneric embryo transfers in which embryos
implanted but did not develop to term: sheep and goat, mouse and rat.
An immunological rejections and placental incompatibility between
the embryo and the surrogate mother appear to restrict interspecific
embryo transfer/interspecific pregnancy. Recently, preimplantation
embryo manipulation procedures have been applied, such as
technique of inner cell mass transfer. This technique will possible to
overcome the reproductive barrier interspecific embryo
transfer/interspecific pregnancy, if there is a protective mechanism
which prevents recognition of the foreign fetus by the mother of the
other species
Abstract: The bioassay-guided isolation and purification of an
ethyl acetate extract of Aspergillus terreus MC751 led to the
characterization of butyrolactone I as an antidiabetic and antioxidant.
The antidiabetic activity of butyrolactone I was evaluated by α-
glucosidase and α-amylase inhibition assays. Butyrolactone I
demonstrated significant concentration-dependent, mixed-type
inhibitory activity against yeast α-glucosidase with an IC50 of 54μM.
However, the compound exhibited less activity against rat intestinal
α-glucosidase and α-amylase. This is the first report on α-glucosidase
inhibitory activity of butyrolactone I. The antioxidative activity of
butyrolactone I was evaluated based on scavenging effects on 1,1-
diphenyl-2-picrylhydrazyl (DPPH) (IC50 =51 μM) and hydrogen
peroxide (IC50= 141 μM) radicals as well as a reducing power assay.
The results suggest that butyrolactone I is a promising antidiabetic as
well as antioxidant and should be considered for clinical trials.
Abstract: Carbon nanotubes (CNTs) are attractive because of
their excellent chemical durability mechanical strength and electrical
properties. Therefore there is interest in CNTs for not only electrical
and mechanical application, but also biological and medical
application.
In this study, the dispersion power of surfactant-treated multiwalled
carbon nanotubes (MWCNTs) and their effect on the antibacterial
activity were examined. Surfactant was used sodium
dodecyl-benzenesulfonate (SDBS). UV-vis absorbance and
transmission electron microscopy(TEM) were used to characterize the
dispersion of MWCNTs in the aqueous phase, showing that the
surfactant molecules had been adsorbed onto the MWCNTs surface.
The surfactant-treated MWCNTs exhibited antimicrobial activities
to streptococcus mutans. The optical density growth curves and viable
cell number determined by the plating method suggested that the
antimicrobial activity of surfactant-treated MWCNTs was both
concentration and treatment time-dependent.
Abstract: Twelve lactating Etawah Crossedbred goats were used
in this study. Goat feed consisted of Cally andra callothyrsus,
Pennisetum purpureum, wheat bran and dried fermented cassava
peel. The cassava peels were fermented with a traditional culture
called “ragi tape" (mixed culture of Saccharomyces cerevisae,
Aspergillus sp, Candida, Hasnula and Acetobacter). The goats were
divided into 2 groups (Control and Treated) of six does. The
experimental diet of the Control group consisted of 70% of roughage
(fresh Callyandra callothyrsus and Pennisetum purpureum 60:40)
and 30% of wheat bran on dry matter (DM) base. In the Treated
group 30% of wheat bran was replaced with dried fermented cassava
peels. Data were statistically analyzed using analysis of variance
followed SPSS program. The concentration of HCN in fermented
cassava peel decreased to non toxic level. Nutrient composition of
dried fermented cassava peel consisted of 85.75% dry matter;
5.80% crude protein and 82.51% total digestible nutrien (TDN).
Substitution of 30% of wheat bran with dried fermented cassava peel
in the diet had no effect on dry matter and organic matter intake but
significantly (P< 0.05) decreased crude protein and TDN
consumption as well as milk yields and milk composition. The study
recommended to reduced the level of substitution to less than 30% of
concentrates in the diet in order to avoid low nutrient intake and milk
production of goats.