Adding Olive Oil into Diluents for Improving Semen Quality and Storage Ability of Roosters' Semen during Liquid Storage

The aim of this study was to investigate the effects of supplementing the diluent of roosters' semen with different levels of olive oil on motility, viability, morphology and acrosome integrity of chicken spermatozoa after in vitro storage for up to 72 h. Semen was collected from 60 White Layer males (62 wk of age) kept in separated floor pens and randomly divided into six treatment groups (10 males in each group). Experimental groups were as follows: T1 :fresh semen, T2 : semen extended 1:1 with Al – Daraji 2 diluent (AD2D) alone, T3 – T6 :semen samples extended 1:1 with AD2D supplemented with 2 ml, 4 ml, 6 ml or 8 ml of olive oil / 100 ml of diluent, respectively. Semen samples were then stored at 5 °C for 24 h, 48 h or 72 h. There was a clear influence of diluent supplementation with olive oil on the spermatozoa motility profile; olive oil groups (T3, T4, T5 and T6) recorded the highest scores of mass activity and individual motility during all storage periods compared to T1 and T2 groups. In addition, the inclusion of olive oil into semen diluent (T3, T4, T5 and T6) gave significantly higher percentages of viable spermatozoa, normal morphologically spermatozoa and intact acrosomes irrespective of storage period. These results clearly show that supplementation the diluent of roosters' semen with olive oil can improve semen quality when semen samples in vitro stored at 5 °C for up to 72 h.

Authors:

• Hazim J. Al-Daraji

Keywords:

• Olive oil
• diluent
• liquid storage
• semen quality of roosters.

References:

[1] Parthasarathy, S., N. Santanam, S. Ramachaandra, and O. Meilhac, "
Oxidants and antioxidants in atherogenesis: an appraisal," J. Lipid Res.,
vol. 40, pp. 2143- 2157, 1999.
[2] Esterbauer, H., J. Gebiki, H. Puhl, and G. Jurgens, " The role of lipid
peroxidation and antioxidants in oxidative modification of LDL," Free
Rad. Biol. Med., vol. 13, pp. 341-350, 1992.
[3] Cerolini, S., K. A. Kelso, R. C. Noble, B. K. Speake, F.Pizzi, and L. G.
Cavalchini, " Relationship between spermatozoan lipid composition and
fertility during aging of chickens," Biol. Reprod., vol., 57, pp. 976-980,
1997.
[4] Blesbois, E., M. Lessire, I. Grasseau, J. M. Hallouis, and D. Hermier, "
Effect of dietary fat on the fatty acid composition and fertilizing ability
of fowl semen," Biol. Reprod., vol. 56, pp. 1216-1220, 1997.
[5] Surai, P. F., G. J. Wishart, R. C. Noble, and B. K. Speake, "The
relationship between the dietary provision of ╬▒ - tocopherol and the
concentration of this vitamin in the semen of chicken: effects on lipid
composition and susceptibility to peroxidation," J. Reprod. Fert.,
vol.110, pp. 47-51, 1997.
[6] Surai, P. F., S. Cerolini, G. J. Wishart, B. K. Spake, R. C. Noble and N.
H. C. Sparks, " Lipid and antioxidant composition of chicken semen and
its susceptibility to peroxidation," Poultry and Avian Biology Reviews,
vol.9, no. 1, pp. 11-23, 1998.
[7] Wishart, G. J, "Liquid semen storage: current status and where do we go
from here," WPC, XXII World's Poultry Congress, Istanbul, Turkey,
2004.
[8] Kelso, K. A., S. Cerolini, R. C. Noble, N. H. C. Sparks, and B. K.
Speake, "Lipid and antioxidant changes in semen of broiler fowl from 25
to 60 weeks of age, J. Reprod. Fert., vol. 106, pp. 201-206, 1996.
[9] Duthie, C., and A. Crozier, "Plant - derived phenolic antioxidants,"
Current Opinion Clinical Nutrition and Metabolic Care, vol. 3, pp. 447-
451, 2000.
[10] Braga, C., C. La Vecchia, S. Franceschi, E. Negri, M. Parpinel, A.
Decarli, A. Giacosa, and D. Trichopoulos, " Olive oil, other seasoning
fats, and the risk of colorectal carcinoma," Cancer, vol. 82, pp. 448 -
453, 1998.
[11] Visioli, F., and C. Galli, "The effect of minor constituents of olive oil on
cardiovascular disease: new findings," Nutr. Rev., vol. 56, pp. 142-147,
1998.
[12] D'Angelo, S., C. Manna, V. Migliardi, O. Mazzoni, P. Morrica, G.
Capasso, G. Pontoni, P. Galleti, and V. Zappia, " Pharmacokinetics and
metabolism of hydroxytyrosol, a natural antioxidant from olive oil,"
DMD, vol. 29, no. 11, pp. 1492-1498, 2001.
[13] Burrows, W. H., and J. P. Quinn, "The collection of spermatozoa from
the domestic fowl and turkey," Poultry Sci., vol. 16, pp. 19-24, 1937.
[14] Al-Daraji, H. J., " Diluent supplementation with vitamins A, C and E for
improving fertilizing ability of indigenous roosters' semen," Patent No.
3195, issued from C. O. S. Q. C., Iraq, 2004.
[15] Sexton, T. J., "Studies on the dilution of turkey semen," Br. Poultry Sci.,
vol. 17, pp. 179-186, 1976.
[16] Al-Daraji, H. J., B. T. O. Al- Tikriti, K. H. Hassan, and A. A. Al- Rawi,
"New techniques for determination the avian spermatozoa
abnormalities," Res. J. Bio. Tech., vol. 4, no. 1, pp. 47 - 64, 2002.
[17] Al-Daraji, H. J., " Effect of ascorbic acid supplementation on
physiological and productive traits of Fawbro broiler breeders' flocks
reared under hot climate," Ph. D. Dissertation, College of Agriculture,
University of Baghdad, I998.
[18] Al-Daraji, H. J., " Effects of holding temperature and time on acrosomal
abnormalities of fowl sperms," Indian J. Anim. Sci., vol. 71, no. 1, pp. 32
- 34, 2001.
[19] SAS. "SAS User's Guide: Statistics Version 6th edn," SAS Inc., Cary,
NC, 1996.
[20] Vissers, M. N., P. L. Zock, R. Leenen, A. J. Roodenburg, K. P. van Putte
and M. B. Katan, " Effect of composition of phenols from olives and
extra virgin olive oil on LDL oxidizability in healthy humans," Free
Radic. Res., vol. 35, no. 5, pp. 619 - 629, 2001.
[21] Giovannini, C., E. Straface, D. Modesti, E. Coni, A. Cantafora, M. De
Vincenzi, W. Malorni, and R. M. Assela, " Tyrosol, the major olive oil
biophenol, protects against oxidized - LDL - induced injury in Caco - 2
cells, J. Nutr., vol. 129, pp. 1269 - 1277, 1999.
[22] Manna, C., P. Galletti, V. Cucciolla, O.Moltedo, A. Leone, and V.
Zappia, " The protective effect of the olive oil polyphenol (3, 4 -
Dihydroxyphenyl) - ethanol counteracts reactive oxygen metabolite -
induced cytotoxicity in Caco - 2 cells," J. Nutr., vol. 127, pp. 286 - 292,
1997.
[23] Zheng, Q. Y., H. Kan, and X. Y. Yang, "Monograph of olive leaf
extract," www. Pureworld. com, 1999.
[24] Masella, R., R. Vari, M. D'Archivio, R. Di Benedetto, P. Matarrese, W.
Malorni, B. Scazzocchio, and C. Giovannini, " Extra virgin olive oil
biophenols inhibit cell - mediated oxidation of LDL by increasing the
mRNA transcription og glutathione - related enzymes," J. Nutr., vol.
134, pp. 785 - 791, 2004.
[25] Rawashdeh, A. Y. A., "Influence of olive oil and Ghee (samen balady)
on serum cholesterol of Jordanians," Pakistan J. Nutr., vol. 6, pp. 270 -
275, 2002.
[26] Aydin, R., M.W.Pariza, and M. E. Cook, "Olive oil prevents the adverse
effects of dietary conjucated linoleic acid on chick hatchability and egg
quality," J. Nutr., vol. 131, pp. 800 - 806, 2001.
[27] Scaccini, M. N., M. D'Aquino, V. Gentili, M. Di Felice, and G. Tomassi,
Effect of dietary oils on lipid peroxidation and on antioxidant parameters
of rat plasma and lipoprotein fractions," J. Lipid Res., vol. 33, pp. 627 -
633, 1992.
[28] Al-Daraji, H. J., " Effect of vitamins A, C or E on quality of fowl semen
stored for 24 hours at 4 ┬░C," Iraqi J. Agric., vol. 7, no. 6, pp.170 - 181,
2002.
[29] Donoghue, A. M., and D. J. Donoghue, "Effects of water - and lipid -
soluble antioxidants on turkey sperm viability, membrane integrity, and
motility during liquid storage," Poultry Sci., vol. 76, pp.1440 - 1445,
1997.