Abstract: The aim of the present study was to examine whether
increasing molybdenum (Mo) concentration affects the growth and
Mo concentration of maize (Zea mays L. cv Norma SC) and
sunflower (Helianthus annuus L. cv Arena PR) seedlings within
laboratory conditions.
In this experiment, calcareous chernozem soil was used and Mo
was supplemented into the soil as ammonium molybdate
[(NH4)6Mo7O24.4H2O] in four different concentrations as follow: 0
(control), 30, 90 and 270 mg·kg-1.
In this study, we found that molybdenum in small amount (30
mg·kg-1) affects positively on growth of maize and sunflower
seedlings, however, higher concentration of Mo reduces the dry
weights of shoots and roots. In the case of maize the highest Mo
treatment (270 mg·kg-1) and in sunflower 90 mg·kg-1 treatment
caused significant reduction in plant growth.
In addition, we observed that molybdenum contents in the roots
and shoots were very low in case of control soil but were
significantly elevated with increasing concentration of Mo treatment.
Only in case of sunflower the highest 270 mg·kg-1 Mo treatment
caused decrease in Mo concentration.
Abstract: Waxapple (Syzygium samarngense Merr.et Perry) is an
important tropical fruit in Taiwan. The famous producing area is
located on the coast in Pingtung County. Land subsidence and climate
change will tend to soil alkalization more seriously. This study was to
evaluate the effects of NaCl in waxapple seedlings. NaCl salinity
reduced waxapple shoot growth; it may due to reducing relative water
content in leaf and new shoot. Leaf Cl and Na concentration were
increased but K, Ca, and Mg content had no significant difference after
irrigated with NaCl for six weeks. In roots, Na and Cl content increase
significantly with 90 mM NaCl treatment, but K, Ca, and Mg content
was reduced. 30-90mM Nacl treatment do not effect K/Na, Ca/Na and
Mg/Na ratio, but decrease significantly in 90mM treatment in roots.
The leaf and root electrolyte leakage were significantly affected by 90
mM NaCl treatment. Suggesting 90mM was optimum concentration
for sieve out other tolerance waxapples verities.
Abstract: The tomato is a very important crop, whose
cultivation in the Mediterranean basin is severely affected by the
phytoparasitic weed Phelipanche ramosa. The semiarid regions of
the world are considered the main areas where this parasitic weed is
established causing heavy infestation as it is able to produce high
numbers of seeds (up to 500,000 per plant), which remain viable for
extended period (more than 20 years). In this paper the results
obtained from eleven treatments in order to control this parasitic
weed including chemical, agronomic, biological and biotechnological
methods compared with the untreated test under two plowing depths
(30 and 50 cm) are reported. The split-plot design with 3 replicates
was adopted. In 2014 a trial was performed in Foggia province
(southern Italy) on processing tomato (cv Docet) grown in the field
infested by Phelipanche ramosa. Tomato seedlings were transplant
on May 5, on a clay-loam soil. During the growing cycle of the
tomato crop, at 56-78 and 92 days after transplantation, the number
of parasitic shoots emerged in each plot was detected. At tomato
harvesting, on August 18, the major quantity-quality yield parameters
were determined (marketable yield, mean weight, dry matter, pH,
soluble solids and color of fruits). All data were subjected to analysis
of variance (ANOVA) and the means were compared by Tukey's test.
Each treatment studied did not provide complete control against
Phelipanche ramosa. However, among the different methods tested,
some of them which Fusarium, gliphosate, radicon biostimulant and
Red Setter tomato cv (improved genotypes obtained by Tilling
technology) under deeper plowing (50 cm depth) proved to mitigate
the virulence of the Phelipanche ramose attacks. It is assumed that
these effects can be improved combining some of these treatments
each other, especially for a gradual and continuing reduction of the
“seed bank” of the parasite in the soil.
Abstract: The present study involved analysis of certain
characteristics of the perennial ryegrass (Lolium perenne L.)
genotypes collected from the natural flora of Ankara, and explores a
correlation among them. In order to evaluate the plants for breeding
purpose as per Turkey's environmental conditions, the perennial
ryegrass plants were collected from natural pasture of Ankara in 2004
and were utilized for the study. Seeds of the collected plants were
sown in pots and seedlings were prepared in a greenhouse. In 2005,
the seedlings were transplanted at 50 × 50 cm2 intervals in
Randomized Complete Blocks Design in an experimental field. In
2007 and 2008, data were recorded from the observations and
measurements of 568 perennial ryegrasses. The plant characteristics,
which were investigated, included re-growth time in spring, color,
density, growth habit, tendency to form inflorescence, time of
inflorescence, plant height, length of upper internode, spike length,
leaf length, leaf width, leaf area, leaf shape, number of spikelets per
spike, seed yield per spike and 1000 grain weight and the correlation
analyses were made using this data. Correlation coefficients were
estimated between all paired combinations of the studied traits. The
yield components exhibited varying trends of association among
themselves. Seed yield per spike showed significant and positive
association with the number of spikelets per spike, 1000 grain weight,
plant height, length of upper internode, spike length, leaf length, leaf
width, leaf area and color, but significant and negative association
with the growth habit and re-growth time in spring.
Abstract: Weed suppression and weeding are necessary measures
for rice cultivation. Weed suppression precedes the process of
weeding. It means suppressing the growth of young weeds and
creating a weed-less environment. If we suppress the growth of weeds,
we can reduce the number of weeds in a paddy field. This would result
in a reduction of the weeding work load.
In this paper, we will show how we developed a weed suppression
robot for the purpose of reducing the weeding work load. The robot
has a laser range finder for autonomous mobility and a robot arm for
weed suppression. It travels along the rice rows without stepping on
and injuring the rice plants in a paddy field. The robot arm applies
force to the weed seedlings and thereby suppresses the growth of
weeds. This paper will explain the methodology of the autonomous
mobile, the experiment in weed suppression, and the method of
controlling the robot’s posture on uneven ground.
Abstract: This study was aimed to investigate the effect of
various organic supplements on growth and development of
Dendrobium discolor’s protocorms and seedlings growth of
Dendrobium Judy Rutz. Protocorms of Dendrobium discolor with 2.0
cm. in diameter and seedlings of Dendrobium Judy Rutz at the same
size (0.5 cm. height) were sub-cultured on Hyponex medium
supplemented with cow milk (CM), soy milk (SM), potato extract
(PE) and peptone (P) for 2 months. The protocorms were developed
to seedlings in all treatments after cultured for 2 months. However,
the best results were found on Hyponex medium supplemented with
P was the best in which the maximum fresh and dry weight and
maximum shoot height were obtained in this treatment statistically
different (p ≤ 0.05) to other treatments. Moreover, Hyponex medium
supplemented with P also stimulated the maximum mean number of
5.7 shoots per explant which also showed statistically different (p ≤
0.05) when compared to other treatments. The results of growth of
Dendrobium Judy Rutz seedlings indicated the medium
supplemented with 100 mL/L PE enhanced the maximum fresh and
dry weigh per explants with significantly different (p ≤ 0.05) in fresh
weight from other treatments including the control medium without
any organic supplementation. However, the dry weight was not
significantly different (p ≤ 0.05) from medium supplemented with
SM and P. There was multiple shoots induction in all media with or
without organic supplementation ranging from 2.6 to 3 shoots per
explants. The maximum shoot height was also obtained in the
seedlings cultured on medium supplemented with PE while the
longest root length was found in medium supplemented with SM.
Abstract: This study aimed to investigate effect of different organic supplements on growth of Vanda and Mokara seedlings. Vanda and Mokara seedlings approximately 0.2 and 0.3 cm. in height were sub-cultured onto VW supplemented with 150 ml/L coconut water, 100 g/L potato extract, 100 g/L ‘Gros Michel’ banana (AAA group) and 100 g/L ‘Namwa’ banana (ABB group). The explants were sub-cultured onto the same medium every month for 3 months. The best medium increased stem height to 0.52 and 0.44 Cm. in Vanda and Mokara respectively was supplemented with coconut water. The maximum fresh weight of Vanda (0.59 g) was found on medium supplemented with ‘Gros Michel’ banana while Mokara cultured on medium supplemented with Potato extract had the maximum fresh weight (0.27 g) and number of roots (5.20 roots/shoot) statistically different (p≤ 0.05) to other treatments. However, Vanda cultured on medium supplemented with ‘Namwa’ banana had the maximum number of roots (3.80 roots/shoot). Our results suggested that growth of different orchid genera was responded diversely to different organic supplements.
Abstract: The use of plastic materials in agriculture causes
serious hazards to the environment. The introduction of biodegradable materials, which can be disposed directly into the soil
can be one possible solution to this problem. In the present research results of experimental tests carried out on biodegradable film
fabricated from natural waste (corn husk) are presented. The film was
characterized by Fourier transform infrared spectroscopy (FTIR),
differential scanning calorimeter (DSC), thermal gravimetric analysis
(TGA) and atomic force microscope (AFM) observation. The film is
shown to be readily degraded within 7-9 months under controlled soil
conditions, indicating a high biodegradability rate. The film
fabricated was use to produce biodegradable pot (BioPot) for
seedlings plantation. The introduction and the expanding use of
biodegradable materials represent a really promising alternative for
enhancing sustainable and environmentally friendly agricultural
activities.
Abstract: Polypropylene blended with natural oil and pigment additives has been studied. Different formulations for each compound were made into polybag used for cultivation of oil palm seedlings for strength and mechanical properties studies. One group of sample was exposed under normal sunlight to initiate degradation and another group of sample was placed under shaded area for five months. All samples were tested for tensile strength to determine the degradation effects. The tensile strength of directly exposed sunlight samples and shaded area showed up to 50% and 25% degradation respectively. However, similar reduction of Young’s modulus for all samples was found for both exposures. Structural investigations were done using FTIR to detect deformation. The natural additives that were used in the studies were all natural and environmental friendly
Abstract: Cabbage seedlings grown in vitro were exposed to
excess levels of heavy metals, including Cd, Mo, and Zn. High metal
levels affected plant growth at cotyledonary stage. Seedlings under
Cd, Mo, and Zn treatments could not produce root hairs and true
leaves. Under stress conditions, seedlings accumulated a higher
amount of anthocyanins in their cotyledons than those in the control.
The pigments isolated from Cd and Zn stressed seedling cotyledons
appeared as pink, while under Mo stress, was dark pink or purple.
Moreover, excess Mo stress increased antioxidant enzyme activities
of APX, CAT, SOD. These results suggest that, under excess Mo
stress, the induced antioxidant enzyme activity of cabbage seedlings
may function as a protective mechanism to shield the plants from
toxicity and exacerbated growth.
Abstract: Principally, plants grown in soilless culture may be
attacked by the same pests and diseases as cultivated traditionally in
soil. The most destructive phytopathogens are fungi, such as
Phythium, Phytophthora and Fusarium, followed by viruses, bacteria
and nematodes. We investigated effect of carbon nanotube filters on
disease management of soilless culture. Tomato seedlings transplant
in plastic pots filled with a soilless media of vermiculite. The crop
irrigated and fertilized using a hydroponic nutrient solution. We used
carbon nanotube filters for nutrient solution disinfection. Our results
show that carbon nanotube filtration significantly reduces pathogens
on tomato plants. Fungal elimination (Fusarium oxysporum and
Pythium spp.) was usually successful at about 96 to 99.9% all over
the cultural season. It is seem that in tomato soilless culture,
nanofiltration constitutes a reliable method that allows control of the
development of diseases caused by pathogenic fungi
Abstract: The comparative analysis of different taxonomic
groups of microorganisms isolated from dark chernozem soils under
different agricultures (alfalfa, melilot, sainfoin, soybean, rapeseed) at
Almaty region of Kazakhstan was conducted. It was shown that the
greatest number of micromycetes was typical to the soil planted with
alfalfa and canola. Species diversity of micromycetes markedly
decreases as it approaches the surface of the root, so that the species
composition in the rhizosphere is much more uniform than in the
virgin soil. Promising strains of microscopic fungi and yeast with
plant growth-promoting activity to agricultures were selected. Among
the selected fungi there are representatives of Penicillium bilaiae,
Trichoderma koningii, Fusarium equiseti, Aspergillus ustus. The
highest rates of growth and development of seedlings of plants
observed under the influence of yeasts Aureobasidium pullulans,
Rhodotorula mucilaginosa, Metschnikovia pulcherrima. Using
molecular - genetic techniques confirmation of the identification
results of selected micromycetes was conducted.
Abstract: In a lowland dipterocarp forest, we assessed the impact of canopy openness (CO) and the resultant changes under different logging systems using hemispherical photography. CO was assessed in a primary forest and two forests logged selectively using reduced impact logging. At one site, 3-m-wide strip cutting was conducted for line planting. From the comparison of CO among the three sites, we found significant changes caused by logging. However, no significant difference was observed between the two logged sites. Strip cutting treatment did not affect CO. One year after, significant canopy closure occurred in both of the logged sites. Canopy closure was significant regardless of the disturbance element, logging gap, skid trail, or strip cutting line. Significant establishment of seedlings within a year was observed in the strip cutting line. Seedling establishment seemed to contribute to rapid canopy closure and prospected to affect to the survival and growth of planted trees.
Abstract: In conventional seedling production, the seedlings are
being grown in the open field under natural conditions. Here they are
susceptible to sudden changes in climate were their quality and yield
is affected. Quality seedlings are essential for good growth and
performance of crops in main field; they serve as a foundation for the
economic returns to the farmer. Producing quality seedling demands
usage of hybrid seeds as they have the ability to result in better yield,
greater uniformity, improved color, disease resistance, and so forth.
Hybrid seed production poses major operational challenge and its
seed use efficiency plays an important role. Thus in order to
overcome the difficulties currently present in conventional seedling
production and to efficiently use hybrid seeds, ITC Limited Agri
Business Divisions - Sustainability Cell as conceptualized a novel
method of seedling production unit for farmers in West Godavari
District of Andhra Pradesh. The “Green House based Float Seedling"
methodology aims at a protected cultivation technique wherein the
micro climate surrounding the plant/seedling body is controlled
partially or fully as per the requirement of the species. This paper
reports on the techno economic evaluation of green house for
cultivation of float based seedling production with experimental
results that was attained from the pilot implementation in West
Godavari District, Rajahmundry region of India.
Abstract: Humic acids (HAs) have been shown to activate some
ion uptakes along with stimulating the lateral roots at effective
concentration of micronutrients. However, the effects of HA on ion
adsorption by plant roots are not easily explainable due to the
varieties of HAs that differ from origins. Therefore, this study was
aimed to investigate the effect of various concentrations of HA
obtained from the compost derived from mix manures and some
agricultural wastes on the growth of eggplant seedlings (Solanum
melongena L. cv. Chao Praya) in tissue cultures at low nutrient level.
Egg plant seeds were surfaced sterilized and germinated in ½
Murashige and Skoog medium (MS) without HA added or in ¼ MS
supplemented with 0, 25, 50, 75 and 100 ppm of HAs. Then, they
were cultured for 4 weeks under the controlled environment. The
results showed that seedlings grown on ¼MS supplemented with
HAs at the concentration of 25 and 50 ppm had the average plant
heights (2.49 and 2.28 cm, respectively) higher than the other
treatments. Both treatments also significantly showed the maximum
average fresh and dry weights (p
Abstract: Phytotoxicity of Daphne gnidium L. was evaluated
through the effect of incorporating leaves, stems and roots biomass
into soil (at 12.5, 25, 50g/Kg) and irrigation by their aqueous extracts
(50g/L), on the growth of two crops (Lactuca sativa L. and Raphanus
sativus L.) and two weeds (Peaganum harmala L. and Scolymus
maculatus L.). Results revealed a perceptible phytotoxic effect which
increased with dose and concentration. At the highest dose, roots and
leaves residues was the most toxic and caused total inhibition
respectively, for lettuce and thistle seedling growth. Irrigation with
aqueous extracts of D. gnidium different organs decreased also
seedlings length of all test species. Stems extract was more inhibitor
on thistle than peganum seedling growth; it induced a significant
reduction of 80% and 67%, for, respectively, roots and shoots.
Results of the present study suggest that different organs of D.
gnidium could be exploited in the management of agro-ecosystems.
Abstract: Perennial ryegrass (Lolium perenne L.) plants are cultivated for lawn constitution and as forage plants. Considerable number of perennial ryegrass genotypes are present in the flora of our country and they present substantial was performed based on a Project supported bu TUBITAK (Project numver : 106O159) and perannial ryegrass genotypes from 8 provinces were collected during 2006. Seeds of perennial ryegrass were collected from 48 different locations. Populations of turfgrass seeds in flowerpots to be 20 and 1 cm deep greenhouse were sown in three replications at 07.07.2007.Then the growth of turfgrass seedlings in the greenhouse in pots showed sufficiently separated from the plants were planted in each population. Plants planted in the garden of the observation scale of 1-9 was evaluated by the quality, 1 = the weakest / worst, 6 = acceptable and 9 = superior or considered as an ideal. Essentially only recognized in assessing the quality of the color of grass, but the color, density, uniformity, texture (texture), illness or environmental stresses are evaluated as a combination reaction. Turfgrass quality 15.11.2007, 19.03.2008, 27.05.2008, 27.11.2008, 07.03.2009 and 02.06.2009 have been 6 times to be in order. Observations made regarding the quality of grass; 3 years according to seasonal environments turf quality genotypes belonging to 14 different populations were found to be 7.5 and above are reserved for future use in breeding works.The number of genotypes belonging to 41 populations in terms of turfgrass quality was determined as 7.9 of 3 year average seasonal. Argıthan between Doğanhisar (Konya) is located 38.09 latitude and 31.40 longitude, altitude 1158 m in the set that population numbered 41.
Abstract: Pentachlorophenol (PCP) is a polychlorinated
aromatic compound that is widespread in industrial effluents and is
considered to be a serious pollutant. Among the variety of industrial
effluents encountered, effluents from tanning industry are very
important and have a serious pollution potential. PCP is also formed
unintentionally in effluents of paper and pulp industries. It is highly
persistent in soils and is lethal to a wide variety of beneficial
microorganisms and insects, human beings and animals. The natural
processes that breakdown toxic chemicals in the environment have
become the focus of much attention to develop safe and environmentfriendly
deactivation technologies. Microbes and plants are among
the most important biological agents that remove and degrade waste
materials to enable their recycling in the environment. The present
investigation was carried out with the aim of developing a microbial
system for bioremediation of PCP polluted soils. A number of plant
species were evaluated for their ability to tolerate different
concentrations of pentachlorophenol (PCP) in the soil. The
experiment was conducted for 30 days under pot culture conditions.
The toxic effect of PCP on plants was studied by monitoring seed
germination, plant growth and biomass. As the concentration of PCP
was increased to 50 ppm, the inhibition of seed germination, plant
growth and biomass was also increased. Although PCP had a
negative effect on all plant species tested, maize and groundnut
showed the maximum tolerance to PCP. Other tolerating crops
included wheat, safflower, sunflower, and soybean. From the
rhizosphere soil of the tolerant seedlings, as many as twenty seven
PCP tolerant bacteria were isolated. From soybean, 8; sunflower, 3;
safflower 8; maize 2; groundnut and wheat, 3 each isolates were
made. They were screened for their PCP degradation potentials.
HPLC analyses of PCP degradation revealed that the isolate MAZ-2
degraded PCP completely. The isolate MAZ-1 was the next best
isolate with 90 per cent PCP degradation. These strains hold promise
to be used in the bioremediation of PCP polluted soils.
Abstract: The cDNA encoding the 326 amino acids of a Class I
basic chitinase gene from Leucaena leucocephala de Wit (KB3,
Genbank accession: AAM49597) was cloned under the control of
CaMV35S promoter in pCAMBIA 1300 and transferred to
Koshihikari. Calli of Koshihikari rice was transformed with
agrobacterium with this construct expressing the chitinase and β-
glucouronidase (GUS). The frequencies of calli 90 % has been
obtained from rice seedlings cultured on NB medium. The high
regeneration frequencies, 74% was obtained from calli cultured on
regeneration medium containing 4 mg/l BAP, and 7 g/l phytagel at
25°C. Various factors were studied in order to establish a procedure
for the transformation of Koshihikari Agrobacterium tumefaciens.
Supplementation of 50 mM acetosyringone to the medium during
coculivation was important to enhance the frequency to transient
transformation. The 4 week-old scutellum-derived calli were
excellent starting materials. Selection medium based on NB medium
supplement with 40 mg/l hygromycin and 400 mg/l cefotaxime were
an optimized medium for selection of transformed rice calli. The
percentage of transformation 70 was obtained. Recombinant calli and
regenerated rice plants were checked the expression of chitinase and
gus by PCR, northern blot gel, southern blot gel, and gus assay.
Chitinase and gus were expressed in all parts of recombinant rice.
The rice line expressing the KB3 chiitnase was more resistant to the
blast fungus Fusarium monoliforme than control line.
Abstract: One problem of synthetic sunflower cultivation is an
erratic germination of the seeds. To improve the germination, presowing
seed treatment with an ultrasound was tested. All treatments
were carried out at 40 kHz frequency with the intensities of 40, 60,
80 and 100% of the ultrasonic generator total power (250 W) for the
durations of 5, 10, 15 and 20 minutes. Data on seed germination
percentage, seed vigor index (SVI), root and shoot lengths of
seedlings were collected. The results showed that germination, SVI,
root and shoot lengths of ultrasonic treated seedlings were different
from the control, depending on intensity of the ultrasound. The
effects of ultrasonic treatment were significant on germination,
resulting in a maximum increase of 43% at 40 and 60% intensities
compared to that of the control seeds. In addition, seedlings of these 2
treatments had higher SVI and longer root and shoot lengths than that
of the control seedlings. All treatment durations resulted in higher
germination and SVI, longer root and higher shoot lenghts of
seedlings than the control. Among the duration treatments, only SVI
and seedling root length were significantly different.