Abstract: Plants as therapeutic agents are used as drug in many parts of the world. Medicinal plants are mostly used in developing countries due to culture acceptability, belief or due to lack of easy access to primary health care services. Jatropha curcas is a plant from the Euphorbiaceae family which is widely used in Northern Nigeria as an anti-diarrheal agent. This study was conducted to determine the anti-diarrheal effect of the leaf extract on castor oil induced diarrhea in albino rats. The leaves of J. curcas were collected from Balanga Local government in Gombe State, north-eastern Nigeria; due to its bioavailability. The leaves were air-dried at room temperature and ground to powder. Phytochemical screening was done and different concentrations of the extract was prepared and administered to the different categories of experimental animals. From the results, aqueous leaf extract of Jatropha curcas at doses of 200mg/Kg and 400mg/Kg was found to reduce the mean stool score as compared to control rats, however, maximum reduction was achieved with the standard drug of Loperamide (5mg/Kg). Treatment of diarrhea with 200mg/Kg of the extract did not produce any significant decrease in stool fluid content but was found to be significant in those rats that were treated with 400mg/Kg of the extract at 2hours (0.05±0.02) and 4hours (0.01±0.01). A significant reduction of diarrhea in the experimental animals signifies it to possess some anti-diarrheal activity.
Abstract: Following plants-barley (Hordeum sativum), alfalfa
(Medicago sativa), grass mixture (red fescue-75%, long-term
ryegrass - 20% Kentucky bluegrass - 10%), oilseed rape (Brassica
napus biennis), resistant to growth in the contaminated soil with oil
content of 15.8 g / kg 25.9 g / kg soil were used. Analysis of the
population showed that the oil pollution reduces the number of
bacteria in the rhizosphere and rhizoplane of plants and enhances the
amount of spore-forming bacteria and saprotrophic micromycetes. It
was shown that regardless of the plant, dominance of Pseudomonas
and Bacillus genera bacteria was typical for the rhizosphere and
rhizoplane of plants. The frequency of bacteria of these genera was
more than 60%. Oil pollution changes the ratio of occurrence of
various types of bacteria in the rhizosphere and rhizoplane of plants.
Besides the Pseudomonas and Bacillus genera, in the presence of
hydrocarbons in the root zone of plants dominant and most typical
were the representatives of the Mycobacterium and Rhodococcus
genera. Together the number was between 62% to 72%.
Abstract: This paper presents results of numerical and experimental studies on a two-dimensional (2D) flapping elliptic airfoil in a forward flight condition at Reynolds number of 5000. The study is motivated from an earlier investigation which shows that the deterioration in thrust performance of a sinusoidal heaving and pitching 2D (NACA0012) airfoil at high flapping frequency can be recovered by changing the effective angle of attack profile to square wave, sawtooth, or cosine wave shape. To better understand why such modifications lead to superior thrust performance, we take a closer look at the transient aerodynamic force behavior of an airfoil when the effective angle of attack profile changes gradually from a generic smooth trapezoidal profile to a sinusoid shape by modifying the base length of the trapezoid. The choice of using a smooth trapezoidal profile is to avoid the infinite acceleration condition encountered in the square wave profile. Our results show that the enhancement in the time-averaged thrust performance at high flapping frequency can be attributed to the delay and reduction in the drag producing valley region in the transient thrust force coefficient when the effective angle of attack profile changes from sinusoidal to trapezoidal.
Abstract: Medicinal and aromatic plants are promising and are characterized by the biosynthesis of odorous molecules that make up the so-called essential oils (EO), which have long been known for their antiseptic and therapeutic activity in folk medicine. Essential oils have many therapeutic properties. In herbal medicine, they are used for their antiseptic properties against infectious diseases of fungal origin, against dermatophytes, those of bacterial origin. The objective of this study was to evaluate the influence of drying in the shade on the water content and on the content of essential oils extracted from leaves of Eucalyptus camendulensis for better quality control of medicinal and aromatic plants. The water content of the Eucalyptus camendulensis plant material decreases during the drying process. It decreased from 100% to 0.006% for the drying in the shade after ten days. The moisture content is practically constant at the end of the drying period. The drying in the shade increases the concentration of essential oils of Eucalyptus camendulensis. When the leaves of Eucalyptus camendulensis plant are in the shade, the maximum of the essential oil content was obtained on the eighth day, the recorded value was 1.43% ± 0.01%. Beyond these periods, the content continuously drop in before stabilizing. The optimum drying time is between 6 and 9 days.
Abstract: This study focuses on the stress analysis of Mandibular
Advancement Devices (MADs), which are considered as a standard
treatment of snoring that promoted by American Academy of Sleep
Medicine (AASM). Snoring is the most significant feature of
sleep-disordered breathing (SDB). SDB will lead to serious problems
in human health. Oral appliances are ensured in therapeutic effect and
compliance, especially the MADs. This paper proposes a new MAD
design, and the finite element analysis (FEA) is introduced to precede
the stress simulation for this MAD.
Abstract: The main cause of Alzheimer disease (AD) was
believed to be mainly due to the accumulation of free radicals owing
to oxidative stress (OS) in brain tissue. The mechanism of the
neurotoxicity of Aluminum chloride (AlCl3) induced AD in
hippocampus Albino wister rat brain tissue, the curative & the
protective effects of Lipidium sativum group (LS) water extract were
assessed after 8 weeks by attenuated total reflection spectroscopy
ATR-IR and histologically by light microscope. ATR-IR results
revealed that the membrane phospholipid undergo free radical
attacks, mediated by AlCl3, primary affects the polyunsaturated fatty
acids indicated by the increased of the olefinic -C=CH sub-band area
around 3012 cm-1 from the curve fitting analysis. The narrowing in
the half band width (HBW) of the sνCH2 sub-band around 2852 cm-1
due to Al intoxication indicates the presence of trans form fatty acids
rather than gauch rotomer. The degradation of hydrocarbon chain to
shorter chain length, increasing in membrane fluidity, disorder, and
decreasing in lipid polarity in AlCl3 group indicated by the detected
changes in certain calculated area ratios compared to the control.
Administration of LS was greatly improved these parameters
compared to the AlCl3 group. Al influences the Aβ aggregation and
plaque formation, which in turn interferes to and disrupts the
membrane structure. The results also showed a marked increase in
the β-parallel and antiparallel structure, that characterize the Aβ
formation in Al-induced AD hippocampal brain tissue, indicated by
the detected increase in both amide I sub-bands around 1674, 1692
cm-1. This drastic increase in Aβ formation was greatly reduced in the
curative and protective groups compared to the AlCl3 group and
approached nearly the control values. These results supported too by
the light microscope. AlCl3 group showed significant marked
degenerative changes in hippocampal neurons. Most cells appeared
small, shrieked and deformed. Interestingly, the administration of LS
in curative and protective groups markedly decreases the amount of
degenerated cells compared to the non-treated group. In addition, the
intensity of congo red stained cells was decreased. Hippocampal
neurons looked more/or less similar to those of control. This study showed a promising therapeutic effect of Lipidium
sativum group (LS) on AD rat model that seriously overcome the
signs of oxidative stress on membrane lipid and restore the protein
misfolding.
Abstract: According to IR, 13C and 1H NMR, APT, 1D NOE,
2D heteronuclear 1H/13C HSQC and 2D DOSY experiments the main
chemical constituent of high-molecular preparations from Symphytum
asperum, S. caucasicum, S. officinale and Anchusa italica
(Boraginaceae) was found to be caffeic acid-derived polyether,
namely poly[3-(3,4-dihydroxyphenyl)glyceric acid] (PDPGA) or
poly[oxy-1-carboxy-2-(3,4-dihydroxyphenyl)ethylene]. Most
carboxylic groups of this polymer of A. italica are methylated.
Abstract: Cancer is still one of the serious diseases threatening
the lives of human beings. How to have an early diagnosis and
effective treatment for tumors is a very important issue. The animal
carcinoma model can provide a simulation tool for the studies of
pathogenesis, biological characteristics, and therapeutic effects.
Recently, drug delivery systems have been rapidly developed to
effectively improve the therapeutic effects. Liposome plays an
increasingly important role in clinical diagnosis and therapy for
delivering a pharmaceutic or contrast agent to the targeted sites.
Liposome can be absorbed and excreted by the human body, and is
well known that no harm to the human body. This study aimed to
compare the therapeutic effects between encapsulated (doxorubicin
liposomal, Lipodox) and un-encapsulated (doxorubicin, Dox)
anti-tumor drugs using magnetic resonance imaging (MRI).
Twenty-four New Zealand rabbits implanted with VX2 carcinoma at
left thighs were classified into three groups: control group (untreated),
Dox-treated group, and LipoDox-treated group, 8 rabbits for each
group. MRI scans were performed three days after tumor implantation.
A 1.5T GE Signa HDxt whole body MRI scanner with a high
resolution knee coil was used in this study. After a 3-plane localizer
scan was performed, three-dimensional (3D) fast spin echo (FSE)
T2-weighted Images (T2WI) was used for tumor volumetric
quantification. Afterwards, two-dimensional (2D) spoiled gradient
recalled echo (SPGR) dynamic contrast-enhanced (DCE) MRI was
used for tumor perfusion evaluation. DCE-MRI was designed to
acquire four baseline images, followed by contrast agent Gd-DOTA
injection through the ear vein of rabbit. A series of 32 images were
acquired to observe the signals change over time in the tumor and
muscle. The MRI scanning was scheduled on a weekly basis for a
period of four weeks to observe the tumor progression longitudinally.
The Dox and LipoDox treatments were prescribed 3 times in the first
week immediately after the first MRI scan; i.e. 3 days after VX2 tumor
implantation. ImageJ was used to quantitate tumor volume and time
course signal enhancement on DCE images. The changes of tumor size
showed that the growth of VX2 tumors was effectively inhibited for
both LipoDox-treated and Dox-treated groups. Furthermore, the tumor
volume of LipoDox-treated group was significantly lower than that of
Dox-treated group, which implies that LipoDox has better therapeutic effect than Dox. The signal intensity of LipoDox-treated group is
significantly lower than that of the other two groups, which implies
that targeted therapeutic drug remained in the tumor tissue. This study
provides a radiation-free and non-invasive MRI method for
therapeutic monitoring of targeted liposome on an animal tumor
model.
Abstract: Abstract—[Tris (1,10-phenanthroline) lanthanum(III)]
trithiocyanate is a new compound that has shown high ability for
stopping the synthesis of DNA and also acting as a photosensitizer.
Nowadays, the radiation dose assessment resource (RADAR) method
is known as the most common method for absorbed dose calculation.
177Lu was produced by (n, gamma) reaction in a research reactor.
177Lu-PL3 was prepared in the optimized condition. The
radiochemical yield was checked by ITLC method. The
biodistribution of the complex was investigated by intravenously
injection to wild-type rats via their tail veins. In this study, the
absorbed dose of 177Lu-PL3 to human organs was estimated by
RADAR method. 177Lu was prepared with a specific activity of 2.6-3
GBq.mg-1 and radionuclide purity of 99.98 %. Final preparation of
the radiolabelled complex showed high radiochemical purity of >
99%. The results show that liver and spleen have received the highest
absorbed dose of 1.051 and 0.441 mSv/MBq, respectively. The
absorbed dose values for these two dose-limiting tissues suggest
more biological studies special in tumor-bearing animals.
Abstract: Cholera is a disease that is predominately common in
developing countries due to poor sanitation and overcrowding
population. In this paper, a deterministic model for the dynamics of
cholera is developed and control measures such as health educational
message, therapeutic treatment, and vaccination are incorporated in
the model. The effective reproduction number is computed in terms
of the model parameters. The existence and stability of the
equilibrium states, disease free and endemic equilibrium states are
established and showed to be locally and globally asymptotically
stable when R0 < 1 and R0 > 1 respectively. The existence of
backward bifurcation of the model is investigated. Furthermore,
numerical simulation of the model developed is carried out to show
the impact of the control measures and the result indicates that
combined control measures will help to reduce the spread of cholera
in the population.
Abstract: Tannins are a unique category of plant phytochemicals
especially in terms of their vast potential health-benefiting properties.
Researchers have described the capacity of tannins to enhance
glucose uptake and inhibit adipogenesis, thus being potential drugs
for the treatment of non-insulin dependent diabetes mellitus. Thus,
the present research was conducted to find out tannin content of food
products. The percentage of tannin in various analyzed sources
ranged from 0.0 to 108.53%; highest in kathaa and lowest in ker and
mango bark. The percentage of tannins present in the plants,
however, varies. Numerous studies have confirmed that the naturally
occurring polyphenols are key factor for the beneficial effects of the
herbal medicines. Isolation and identification of active constituents
from plants, preparation of standardized dose & dosage regimen can
play a significant role in improving the hypoglycaemic action.
Abstract: Consumers are demanding novel beverages that are
healthier, convenient and have appealing consumer acceptance. The
objectives of this study were to investigate the effects of adding grape
polyphenols and the influence of presenting health claims on the
sensory acceptability of wines. Fresh red sorrel calyces were
fermented into wines. The total soluble solids of the pectinase-treated
sorrel puree were from 4°Brix to 23.8°Brix. Polyphenol in the form
of grape pomace extract was added to sorrel wines (w/v) in specified
levels to give 0. 25. 50 and 75 ppm. A focus group comprising of 12
panelists was use to select the level of polyphenol to be added to
sorrel wines for sensory preference The sensory attributed of the
wines which were evaluated were colour, clarity, aroma, flavor,
mouth-feel, sweetness, astringency and overall preference. The sorrel
wine which was most preferred from focus group evaluation was
presented for hedonic rating. In the first stage of hedonic testing, the
sorrel wine was served chilled at 7°C for 24 h prior to sensory
evaluation. Each panelist was provided with a questionnaire and was
asked to rate the wines on colour, aroma, flavor, mouth-feel,
sweetness, astringency and overall acceptability using a 9-point
hedonic scale. In the second stage of hedonic testing, the panelist
were instructed to read a health abstract on the health benefits of
polyphenolic compounds and again to rate sorrel wine with added 25
ppm polyphenol. Paired t-test was used for the analysis of the
influence of presenting health information on polyphenols on hedonic
scoring of sorrel wines. Focus groups found that the addition of
polyphenol addition had no significant effect on sensory color and
aroma but affected clarity and flavor. A 25 ppm wine was liked
moderately in overall acceptability. The presentation of information
on the health benefit of polyphenols in sorrel wines to panelists had
no significant influence on the sensory acceptance of wine. More
than half of panelists would drink this wine now and then. This wine
had color L 19.86±0.68, chroma 2.10±0.12, hue° 16.90 ±3.10 and
alcohol content of 13.0%. The sorrel wine was liked moderately in
overall acceptability with the added polyphenols.
Abstract: While choosing insulating oil, characteristic features
such as thermal cooling, endurance, efficiency and being
environment-friendly should be considered. Mineral oils are referred
as petroleum-based oil. In this study, vegetable oils investigated as an
alternative insulating liquid to mineral oil. Dissipation factor,
breakdown voltage, relative dielectric constant and resistivity
changes with the frequency and voltage of mineral, rapeseed and nut
oils were measured. Experimental studies were performed according
to ASTM D924 and IEC 60156 standards.
Abstract: It is a major challenge to build a bridge superstructure
that has long-term durability and low maintenance requirements. A
solution to this challenge may be to use new materials or to
implement new structural systems. Fiber Reinforced Polymer (FRP)
composites have continued to play an important role in solving some
of persistent problems in infrastructure applications because of its
high specific strength, light weight, and durability. In this study, the
concept of the hybrid FRP-concrete structural systems is applied to a
bridge superstructure. The hybrid FRP-concrete bridge superstructure
is intended to have durable, structurally sound, and cost effective
hybrid system that will take full advantage of the inherent properties
of both FRP materials and concrete. In this study, two hybrid FRP-concrete
bridge systems were investigated. The first system consists
of trapezoidal cell units forming a bridge superstructure. The second
one is formed by arch cells. The two systems rely on using cellular
components to form the core of the bridge superstructure, and an
outer shell to warp around those cells to form the integral unit of the
bridge. Both systems were investigated analytically by using finite
element (FE) analysis. From the rigorous FE studies, it was
concluded that first system is more efficient than the second.
Abstract: This study will examine how the therapeutic factors
(therapeutic catharsis-seeking and game-efficacy of the game player)
and self-construal factors (independent and interdependent
self-construal of the game player) as well as social capital factors
(bonding and bridging social capital of the game player) affect
aggression in the game. Results show that both therapeutic
catharsis-seeking and game self-efficacy are particularly important to
the players since they cause the game players’ aggressive tendencies to
be greatly diminished. Independent self-construal reduces the level of
the players’ aggression. Interestingly enough, the bonding social
capital enhances the level of the players’ aggression, while individuals
with bridging social capital did not show any significant effects. The
results and implications will be discussed herein.
Abstract: More than 3000 plants of notable phyto-therapeutic
value grow in South Africa; these include Cissampelos capensis,
commonly known in Afrikaans as dawidjie or dawidjiewortel. C.
capensis is the most significant and popular medicinal plant used by
the Khoisan as well as other rural groups in the Western region of
South Africa. Its rhizomes are traditionally used to treat male fertility
problems. Yet, no studies have investigated the effects of this plant or
its extracts on human spermatozoa. Therefore, this study aimed at
investigating the effects of C. capensis rhizome extract (CRE)
fractions on ejaculated human spermatozoa in vitro. Spermatozoa
from a total of 77 semen samples were washed with human tubular
fluid medium supplemented with bovine serum albumin (HTF-BSA)
and incubated for 2 hours with 20 μg/ml progesterone (P4) followed
by incubation with different concentrations (0, 0.05, 0.5, 5, 50, 200
μg/ml) of fractionated CRE (F1=0% MeOH, F2=30% MeOH,
F3=60% MeOH and F4=100% MeOH) for 1.5 hours at 37°C. A
sample without addition of CRE fractions served as control. Samples
were analyzed for sperm motility, reactive oxygen species (ROS),
DNA-fragmentation, acrosome reaction and capacitation. Results
showed that F1 resulted in significantly higher values for ROS,
capacitation and hyper-activation compared to F2, F3, and F4 with
P4-stimulated samples generally having higher values. No significant
effect was found for the other parameters. In conclusion, alkaloids
present in F1 of CRE appear to have triggered sperm intrinsic ROS
production leading to sperm capacitation and acrosome reaction
induced by P4.
Abstract: Auditory hallucinations among the most invalidating
and distressing experiences reported by patients diagnosed with
schizophrenia, leading to feelings of powerlessness and helplessness
towards their illness. In more severe cases, these auditory
hallucinations can take the form of commanding voices, which are
often related to high suicidality rates in these patients. Several
authors propose that the meanings attributed to the hallucinatory
experience, rather than characteristics like form and content, can be
determinant in patients’ reactions to hallucinatory activity,
particularly in the case of voice-hearing experiences. In this study, 48
patients diagnosed with paranoid schizophrenia presenting auditory
hallucinations were studied. Multiple regression analyses were
computed to study the influence of several developmental aspects,
such as family and social dynamics, bullying, depression, and sociocognitive
variables on the auditory hallucinations, on patients’
attributions and relationships with their voices, and on the resulting
invalidation of hallucinatory experience. Overall, results showed how
relationships with voices can mirror several aspects of interpersonal
relationship with others, and how self-schemas, depression and actual
social relationships help shaping the voice-hearing experience. Early
experiences of victimization and submission help predict the
attributions of omnipotence of the voices, and increased hostility
from parents seems to increase the malevolence of the voices,
suggesting that socio-cognitive factors can significantly contribute to
the etiology and maintenance of auditory hallucinations. The
understanding of the characteristics of auditory hallucinations and the
relationships patients established with their voices can allow the
development of more promising therapeutic interventions that can be
more effective in decreasing invalidation caused by this devastating
mental illness.
Abstract: Purpose: The study aimed to assess the depressant or
antidepressant effects of several Nonsteroidal Anti-Inflammatory
Drugs (NSAIDs) in mice: the selective cyclooxygenase-2 (COX-2)
inhibitor meloxicam, and the non-selective COX-1 and COX-2
inhibitors lornoxicam, sodium metamizole, and ketorolac. The
current literature data regarding such effects of these agents are
scarce.
Materials and methods: The study was carried out on NMRI mice
weighing 20-35 g, kept in a standard laboratory environment. The
study was approved by the Ethics Committee of the University of
Medicine and Pharmacy „Carol Davila”, Bucharest. The study agents
were injected intraperitoneally, 10 mL/kg body weight (bw) 1 hour
before the assessment of the locomotor activity by cage testing (n=10
mice/ group) and 2 hours before the forced swimming tests (n=15).
The study agents were dissolved in normal saline (meloxicam,
sodium metamizole), ethanol 11.8% v/v in normal saline (ketorolac),
or water (lornoxicam), respectively. Negative and positive control
agents were also given (amitryptilline in the forced swimming test).
The cage floor used in the locomotor activity assessment was divided
into 20 equal 10 cm squares. The forced swimming test involved
partial immersion of the mice in cylinders (15/9cm height/diameter)
filled with water (10 cm depth at 28C), where they were left for 6
minutes. The cage endpoint used in the locomotor activity assessment
was the number of treaded squares. Four endpoints were used in the
forced swimming test (immobility latency for the entire 6 minutes,
and immobility, swimming, and climbing scores for the final 4
minutes of the swimming session), recorded by an observer that was
„blinded” to the experimental design. The statistical analysis used the
Levene test for variance homogeneity, ANOVA and post-hoc
analysis as appropriate, Tukey or Tamhane tests.
Results: No statistically significant increase or decrease in the
number of treaded squares was seen in the locomotor activity
assessment of any mice group. In the forced swimming test,
amitryptilline showed an antidepressant effect in each experiment, at
the 10 mg/kg bw dosage. Sodium metamizole was depressant at 100
mg/kg bw (increased the immobility score, p=0.049, Tamhane test),
but not in lower dosages as well (25 and 50 mg/kg bw). Ketorolac
showed an antidepressant effect at the intermediate dosage of 5
mg/kg bw, but not so in the dosages of 2.5 and 10 mg/kg bw,
respectively (increased the swimming score, p=0.012, Tamhane test).
Meloxicam and lornoxicam did not alter the forced swimming
endpoints at any dosage level.
Discussion: 1) Certain NSAIDs caused changes in the forced
swimming patterns without interfering with locomotion. 2) Sodium
metamizole showed a depressant effect, whereas ketorolac proved
antidepressant. Conclusion: NSAID-induced mood changes are not
class effects of these agents and apparently are independent of the
type of inhibited cyclooxygenase (COX-1 or COX-2).
Disclosure: This paper was co-financed from the European Social
Fund, through the Sectorial Operational Programme Human Resources Development 2007-2013, project number POSDRU /159
/1.5 /S /138907 "Excellence in scientific interdisciplinary research,
doctoral and postdoctoral, in the economic, social and medical fields
-EXCELIS", coordinator The Bucharest University of Economic
Studies.
Abstract: Quantitative radiobiological models can be used to
assess the optimum clinical outcome from sophisticated therapeutic
modalities by calculating tumor control probability (TCP) and normal
tissue complication probability (NTCP). In this study two 3D-CRT
and an IMRT treatment plans were developed with an initial
prescription dose of 60 Gy in 2 Gy/fraction to prostate. Sensitivity of
TCP and Complication free tumor control probability (P+) to the
different values of α/β ratio was investigated for various prescription
doses planned to be delivered in either a fixed number of fractions (I)
or in a fixed dose per fraction (II) in each of the three different
treatment plans. High dose/fraction and high α/β value result in
comparatively smaller P+ and IMRT plans resulted in the highest P+,
mainly due to the decrease in NTCP. If α/β is lower than expected,
better tumor control can be achieved by increasing dose/fraction but
decreasing the number of fractions.
Abstract: The research of juice flavor forecasting has become
more important in China. Due to the fast economic growth in China,
many different kinds of juices have been introduced to the market. If a
beverage company can understand their customers’ preference well,
the juice can be served more attractive. Thus, this study intends to
introducing the basic theory and computing process of grapes juice
flavor forecasting based on support vector regression (SVR). Applying
SVR, BPN, and LR to forecast the flavor of grapes juice in real data
shows that SVR is more suitable and effective at predicting
performance.