Biodiversity of Plants Rhizosphere and Rhizoplane Bacteria in the Presence of Petroleum Hydrocarbons
Following plants-barley (Hordeum sativum), alfalfa
(Medicago sativa), grass mixture (red fescue-75%, long-term
ryegrass - 20% Kentucky bluegrass - 10%), oilseed rape (Brassica
napus biennis), resistant to growth in the contaminated soil with oil
content of 15.8 g / kg 25.9 g / kg soil were used. Analysis of the
population showed that the oil pollution reduces the number of
bacteria in the rhizosphere and rhizoplane of plants and enhances the
amount of spore-forming bacteria and saprotrophic micromycetes. It
was shown that regardless of the plant, dominance of Pseudomonas
and Bacillus genera bacteria was typical for the rhizosphere and
rhizoplane of plants. The frequency of bacteria of these genera was
more than 60%. Oil pollution changes the ratio of occurrence of
various types of bacteria in the rhizosphere and rhizoplane of plants.
Besides the Pseudomonas and Bacillus genera, in the presence of
hydrocarbons in the root zone of plants dominant and most typical
were the representatives of the Mycobacterium and Rhodococcus
genera. Together the number was between 62% to 72%.
[1] E. J. Joner, S. C. Corgie, N. Amellal, C. Leyval “Nutritional constraints
to degradation of polycyclic aromatic hydrocarbons in a simulated
rhizosphere”, Soil Biol. Biochem., vol. 34, pp. 859–864, 2002.
[2] Travis S. Walker, Harsh Pal Bais, Erich Grotewold, Jorge M.
Vivanco “Root Exudation and Rhizosphere Biology”, Plant Physiology,
vol. 132, no.1, pp. 44-51, 2003.
[3] Kun Li, Xiu Wu Guo, Hong Gang Xie, Yinshan Guo and Chengxiang Li
“Influence of Rroot Exudates and residues on soil microecological
environment”, Pak. J. Bot., vol. 45, no.5, pp. 1773-1779, 2013.
[4] M. Garcia-Martinez, J. Kuo, K. Kilminster “Microbial colonization in
the seagrass Posidonia spp. Roots”, Mar. Biol. Res., vol. 1, no. 6, pp
388–395, 2005.
[5] N. Narula, E. Kothe, R. Kumar Beh l “Role of root exudates in plantmicrobe
interactions”, J. Appl. Bot. And Food Qual., vol. 82, no.2, pp.
122–130-2009.
[6] María Guineth Torres-Rubio, Sandra Astrid Valencia-Plata, Jaime
Bernal-Castillo, Patricia Martínez-Nieto “Isolation of Enterobacteria,
Azotobacter sp. and Pseudomonas sp., Producers of Indole-3-Acetic
Acid and Siderophores, from Colombian Rice Rhizosphere”, Revista
Latinoamericana de Microbiología, vol 42, pp. 171-176, 2000.
[7] Ajay Kumar, Amit Kumar, Shikha Devi, Sandip Patil, Chandani Payal
“Isolation, screening and characterization of bacteria from Rhizospheric
soils for different plant growth promotion (PGP) activities: an in vitro
study”, Recent Research in Science and Technology, vol. 4, no. 1, pp.
01-05, 2012.
[8] Togzhan D. Mukasheva, Anel A. Omirbekova, Raikhan S. Sydykbekova
et al “Selection of plants as possible rhizoremediators for restoration of
oil contaminated soil”, World Academy of Science, Engineering and
Technology, Issue 79, pp. 1582 – 1586, 2013.
[9] Upadhyay SK, Singh DP, Saikia R. “Genetic diversity of plant growth
promoting rhizobacteria isolated from rhizospheric soil of wheat under
saline condition”, Curr Microbiol, vol. 59, no.5, pp.489-496, 2009.
[10] Ed. F. Gerhardt “Methods for General Bacteriology”. New York:
Wiley,.264 p., 1984.
[11] Dobrovol'skaya TG, Skvortsov IN, Lysak LV “Methods for the
identification and isolation of soil bacteria”, Moscow, Moscow State
University Press, 1990.
[12] Keshav Prasad Shukla, Shivesh Sharma, Nand Kumar Singh, Vasudha
Singh, Kirti Tiwari “Nature and role of root exudates: Efficacy in
bioremediation”, African Journal of Biotechnology, Vol. 10(48), pp.
9717-9724, 2011.
[13] Amann R.I., Ludwig W., Schleifer K.H. “Phylogenic identification and
in situ detection of individual microbial cells without cultivation”,
Microbiol. Rev. ,Vol. 59, № 1, pp.143–169, 199.5
[14] Kennedy A.C., GewinV.L. “Soil microbial diversity: present and future
considerations “, Soil Sci., Vol. 162, № 9, pp. 607-617. 1997.
[15] Torsvik V., Ovreas L. “Microbial diversity and function in soil: from
genus to ecosystems”, Current Opinion in Microbiology, Vol. 5, P. 240–
245, 2002.
[16] Anel Omirbekova , Makpal Kargayeva , Togzhan Mukasheva et al
“Isolation of oil-degrading microorganisms from rhizoplane and
rhizosphere of plants and and evaluation of their destructive activity”,
FEBS Journal Volume 280, Issue Supplement s1.
[17] L. C. Davis et al.”Benefits of vegetation for soils with organic
contaminants”, Critical Reviews in Plant Sciences. Vol. 21, N 5., P.
457–491. 2002.
[18] N. K. Hannik et al. “Phytoremediation of explosives”, Critical Reviews
in Plant Sciences. – 2002. – Vol. 21, N 5. – P. 511–538.
[19] K. Lindström et al. Potential of the Galega–Rhizobium galegae system
for bioremediation of oil-contaminated soil // Food Technol. and
Biotechnol. – 2003. – Vol. 41, N 1. – P. 11–16.
[20] Ho CH, Applegate B, Banks MK. Impact of microbial/plant interactions
on the transformation of polycyclic aromatic hydrocarbons in
rhizosphere of Festuca arundinacea. Int J Phytoremediation. 2007,
9(2):107-14.
[21] Kuiper I, Bloemberg GV, Lugtenberg BJ. Selection of a plant-bacterium
pair as a novel tool for rhizostimulation of polycyclic aromatic
hydrocarbon-degrading bacteria. Mol Plant Microbe Interact. 2001,
14(10):1197-205.
[22] S. A. Ilarionov, A. V. Nazarov, I. G. Kalachnikova The Role of
Micromycetes in the Phytotoxicity of Crude Oil-Polluted Soils Russian
Journal of Ecology Volume 34, Issue 5, pp 303-308
[23] Michael Angelo C. Nicdao and Windell L. Rivera Two strains of
Gordonia terrae isolated from used engine oilcontaminated soil utilize
short - to - long-chain n-alkanes //Philippine science letters. Vol.5. No,
22012.
[24] Chiu-Chung Young, Ta-Chen Lin, Mao-Song Yeh, Fo-Ting Shen, Jo-
Shu “Chang Identification and Kinetic Characteristics of an
Indigenous Diesel-degrading Gordonia alkanivorans Strain”, World
Journal of Microbiology and Biotechnology, v.21, № 8-9, pp 1409-
1414, 2005.
[1] E. J. Joner, S. C. Corgie, N. Amellal, C. Leyval “Nutritional constraints
to degradation of polycyclic aromatic hydrocarbons in a simulated
rhizosphere”, Soil Biol. Biochem., vol. 34, pp. 859–864, 2002.
[2] Travis S. Walker, Harsh Pal Bais, Erich Grotewold, Jorge M.
Vivanco “Root Exudation and Rhizosphere Biology”, Plant Physiology,
vol. 132, no.1, pp. 44-51, 2003.
[3] Kun Li, Xiu Wu Guo, Hong Gang Xie, Yinshan Guo and Chengxiang Li
“Influence of Rroot Exudates and residues on soil microecological
environment”, Pak. J. Bot., vol. 45, no.5, pp. 1773-1779, 2013.
[4] M. Garcia-Martinez, J. Kuo, K. Kilminster “Microbial colonization in
the seagrass Posidonia spp. Roots”, Mar. Biol. Res., vol. 1, no. 6, pp
388–395, 2005.
[5] N. Narula, E. Kothe, R. Kumar Beh l “Role of root exudates in plantmicrobe
interactions”, J. Appl. Bot. And Food Qual., vol. 82, no.2, pp.
122–130-2009.
[6] María Guineth Torres-Rubio, Sandra Astrid Valencia-Plata, Jaime
Bernal-Castillo, Patricia Martínez-Nieto “Isolation of Enterobacteria,
Azotobacter sp. and Pseudomonas sp., Producers of Indole-3-Acetic
Acid and Siderophores, from Colombian Rice Rhizosphere”, Revista
Latinoamericana de Microbiología, vol 42, pp. 171-176, 2000.
[7] Ajay Kumar, Amit Kumar, Shikha Devi, Sandip Patil, Chandani Payal
“Isolation, screening and characterization of bacteria from Rhizospheric
soils for different plant growth promotion (PGP) activities: an in vitro
study”, Recent Research in Science and Technology, vol. 4, no. 1, pp.
01-05, 2012.
[8] Togzhan D. Mukasheva, Anel A. Omirbekova, Raikhan S. Sydykbekova
et al “Selection of plants as possible rhizoremediators for restoration of
oil contaminated soil”, World Academy of Science, Engineering and
Technology, Issue 79, pp. 1582 – 1586, 2013.
[9] Upadhyay SK, Singh DP, Saikia R. “Genetic diversity of plant growth
promoting rhizobacteria isolated from rhizospheric soil of wheat under
saline condition”, Curr Microbiol, vol. 59, no.5, pp.489-496, 2009.
[10] Ed. F. Gerhardt “Methods for General Bacteriology”. New York:
Wiley,.264 p., 1984.
[11] Dobrovol'skaya TG, Skvortsov IN, Lysak LV “Methods for the
identification and isolation of soil bacteria”, Moscow, Moscow State
University Press, 1990.
[12] Keshav Prasad Shukla, Shivesh Sharma, Nand Kumar Singh, Vasudha
Singh, Kirti Tiwari “Nature and role of root exudates: Efficacy in
bioremediation”, African Journal of Biotechnology, Vol. 10(48), pp.
9717-9724, 2011.
[13] Amann R.I., Ludwig W., Schleifer K.H. “Phylogenic identification and
in situ detection of individual microbial cells without cultivation”,
Microbiol. Rev. ,Vol. 59, № 1, pp.143–169, 199.5
[14] Kennedy A.C., GewinV.L. “Soil microbial diversity: present and future
considerations “, Soil Sci., Vol. 162, № 9, pp. 607-617. 1997.
[15] Torsvik V., Ovreas L. “Microbial diversity and function in soil: from
genus to ecosystems”, Current Opinion in Microbiology, Vol. 5, P. 240–
245, 2002.
[16] Anel Omirbekova , Makpal Kargayeva , Togzhan Mukasheva et al
“Isolation of oil-degrading microorganisms from rhizoplane and
rhizosphere of plants and and evaluation of their destructive activity”,
FEBS Journal Volume 280, Issue Supplement s1.
[17] L. C. Davis et al.”Benefits of vegetation for soils with organic
contaminants”, Critical Reviews in Plant Sciences. Vol. 21, N 5., P.
457–491. 2002.
[18] N. K. Hannik et al. “Phytoremediation of explosives”, Critical Reviews
in Plant Sciences. – 2002. – Vol. 21, N 5. – P. 511–538.
[19] K. Lindström et al. Potential of the Galega–Rhizobium galegae system
for bioremediation of oil-contaminated soil // Food Technol. and
Biotechnol. – 2003. – Vol. 41, N 1. – P. 11–16.
[20] Ho CH, Applegate B, Banks MK. Impact of microbial/plant interactions
on the transformation of polycyclic aromatic hydrocarbons in
rhizosphere of Festuca arundinacea. Int J Phytoremediation. 2007,
9(2):107-14.
[21] Kuiper I, Bloemberg GV, Lugtenberg BJ. Selection of a plant-bacterium
pair as a novel tool for rhizostimulation of polycyclic aromatic
hydrocarbon-degrading bacteria. Mol Plant Microbe Interact. 2001,
14(10):1197-205.
[22] S. A. Ilarionov, A. V. Nazarov, I. G. Kalachnikova The Role of
Micromycetes in the Phytotoxicity of Crude Oil-Polluted Soils Russian
Journal of Ecology Volume 34, Issue 5, pp 303-308
[23] Michael Angelo C. Nicdao and Windell L. Rivera Two strains of
Gordonia terrae isolated from used engine oilcontaminated soil utilize
short - to - long-chain n-alkanes //Philippine science letters. Vol.5. No,
22012.
[24] Chiu-Chung Young, Ta-Chen Lin, Mao-Song Yeh, Fo-Ting Shen, Jo-
Shu “Chang Identification and Kinetic Characteristics of an
Indigenous Diesel-degrading Gordonia alkanivorans Strain”, World
Journal of Microbiology and Biotechnology, v.21, № 8-9, pp 1409-
1414, 2005.
@article{"International Journal of Earth, Energy and Environmental Sciences:72073", author = "Togzhan D. Mukasheva and Anel A. Omirbekova and Raikhan S. Sydykbekova and Ramza Zh. Berzhanova and Lyudmila V. Ignatova", title = "Biodiversity of Plants Rhizosphere and Rhizoplane Bacteria in the Presence of Petroleum Hydrocarbons", abstract = "Following plants-barley (Hordeum sativum), alfalfa
(Medicago sativa), grass mixture (red fescue-75%, long-term
ryegrass - 20% Kentucky bluegrass - 10%), oilseed rape (Brassica
napus biennis), resistant to growth in the contaminated soil with oil
content of 15.8 g / kg 25.9 g / kg soil were used. Analysis of the
population showed that the oil pollution reduces the number of
bacteria in the rhizosphere and rhizoplane of plants and enhances the
amount of spore-forming bacteria and saprotrophic micromycetes. It
was shown that regardless of the plant, dominance of Pseudomonas
and Bacillus genera bacteria was typical for the rhizosphere and
rhizoplane of plants. The frequency of bacteria of these genera was
more than 60%. Oil pollution changes the ratio of occurrence of
various types of bacteria in the rhizosphere and rhizoplane of plants.
Besides the Pseudomonas and Bacillus genera, in the presence of
hydrocarbons in the root zone of plants dominant and most typical
were the representatives of the Mycobacterium and Rhodococcus
genera. Together the number was between 62% to 72%.", keywords = "Identification, micromycetes, pollution, root system.", volume = "8", number = "7", pages = "538-5", }
