Abstract: Nonspecific protein adsorption generally occurs on
any solid surfaces and usually has adverse consequences. Adsorption
of proteins onto a solid surface is believed to be the initial and
controlling step in biofouling. Surfaces modified with end-tethered
poly(ethylene glycol) (PEG) have been shown to be protein-resistant
to some degree. In this study, the adsorption of β-casein and
lysozyme was performed on 6 different types of surfaces where PEG
was tethered onto stainless steel by polyethylene imine (PEI) through
either OH or NHS end groups. Protein adsorption was also performed
on the bare stainless steel surface as a control. The adsorption was
conducted at 23 °C and pH 7.2. In situ QCM-D was used to
determine PEG adsorption kinetics, plateau PEG chain densities,
protein adsorption kinetics and plateau protein adsorbed quantities.
PEG grafting density was the highest for a NHS coupled chain,
around 0.5 chains / nm2. Interestingly, lysozyme which has smaller
size than β-casein, appeared to adsorb much less mass than that of β-
casein. Overall, the surface with high PEG grafting density exhibited
a good protein rejection.
Abstract: This research project aims to investigate difference in
relative rates concerning phosphoryl transfer relevant to biological
catalysis of DNA and RNA in the pH-independent reactions.
Activated Models of DNA and RNA for alkyl-aryl phosphate diesters
(with 4-nitrophenyl as a good leaving group) have successfully been
prepared to gather kinetic parameters. Eyring plots for the pH–
independent hydrolysis of 1 and 2 were established at different
temperatures in the range 100–160 °C. These measurements have
been used to provide a better estimate for the difference in relative
rates between the reactivity of DNA and RNA cleavage. Eyring plot
gave an extrapolated rate of kH2O = 1 × 10-10 s -1 for 1 (RNA model)
and 2 (DNA model) at 25°C. Comparing the reactivity of RNA
model and DNA model shows that the difference in relative rates in
the pH-independent reactions is surprisingly very similar at 25°. This
allows us to obtain chemical insights into how biological catalysts
such as enzymes may have evolved to perform their current
functions.
Abstract: In the current work, a numerical parametric study was
performed in order to model the fluid mechanics in the riser of a
bubbling fluidized bed (BFB). The gas-solid flow was simulated by
mean of a multi-fluid Eulerian model incorporating the kinetic theory
for solid particles. The bubbling fluidized bed was simulated two
dimensionally by mean of a Computational Fluid Dynamic (CFD)
commercial software package, Fluent. The effects of using different
inter-phase drag function (the drag model of Gidaspow, Syamlal and
O-Brien and the EMMS drag model) on the model predictions were
evaluated and compared. The results showed that the drag models of
Gidaspow and Syamlal and O-Brien overestimated the drag force for
the FCC particles and predicted a greater bed expansion in
comparison to the EMMS drag model.
Abstract: The rate of nitrate adsorption by a nitrate selective ion
exchange resin was investigated in a well-stirred batch experiments.
The kinetic experimental data were simulated with diffusion models including external mass transfer, particle diffusion and chemical
adsorption. Particle pore volume diffusion and particle surface diffusion were taken into consideration separately and simultaneously
in the modeling. The model equations were solved numerically using the Crank-Nicholson scheme. An optimization technique was
employed to optimize the model parameters. All nitrate concentration
decay data were well described with the all diffusion models. The
results indicated that the kinetic process is initially controlled by external mass transfer and then by particle diffusion. The external
mass transfer coefficient and the coefficients of pore volume diffusion and surface diffusion in all experiments were close to each
other with the average value of 8.3×10-3 cm/S for external mass
transfer coefficient. In addition, the models are more sensitive to the
mass transfer coefficient in comparison with particle diffusion. Moreover, it seems that surface diffusion is the dominant particle
diffusion in comparison with pore volume diffusion.
Abstract: Heavy metals have bad effects on environment and
soils and it can uptake by natural HAP .natural Hap is an inexpensive
material that uptake large amounts of various heavy metals like Zn
(II) .Natural HAP (N-HAP), extracted from bovine cortical bone ash,
is a good choice for substitution of commercial HAP. Several
experiments were done to investigate the sorption capacity of Zn (II)
to N-HAP in various particles sizes, temperatures, initial
concentrations, pH and reaction times. In this study, the sorption of
Zinc ions from a Zn solution onto HAP particles with sizes of 1537.6
nm and 47.6 nm at three initial pH values of 4.50, 6.00 and 7.50 was
studied. The results showed that better performance was obtained
through a 47.6 nm particle size and higher pH values. The
experimental data were analyzed using Langmuir, Freundlich, and
Arrhenius equations for equilibrium, kinetic and thermodynamic
studies. The analysis showed a maximum adsorption capacity of NHAP
as being 1.562 mmol/g at a pH of 7.5 and small particle size.
Kinetically, the prepared N-HAP is a feasible sorbent that retains Zn
(II) ions through a favorable and spontaneous sorption process.
Abstract: Gaharu that produced by Aquilaria spp. is classified as
one of the most valuable forest products traded internationally as it is
very resinous, fragrant and highly valuable heartwood. Gaharu has
been widely used in aromatheraphy, medicine, perfume and religious
practices. This work aimed to determine the factors affecting solid
liquid extraction of gaharu oil using hexane as solvent under
experimental condition. The kinetics of extraction was assumed and
verified based on a second-order mechanism. The effect of three
main factors, which were temperature, reaction time and solvent to
solid ratio were investigated to achieve maximum oil yield. The
optimum condition were found at temperature 65°C, 9 hours reaction
time and solvent to solid ratio of 12:1 with 14.5% oil yield. The
kinetics experimental data agrees and well fitted with the second
order extraction model. The initial extraction rate (h) was 0.0115
gmL-1min-1; the extraction capacity (Cs) was 1.282gmL-1; the second
order extraction constant (k) was 0.007 mLg-1min-1 and coefficient of
determination, R2 was 0.945.
Abstract: This paper presents a computational study of the separated flow in a planer asymmetric diffuser. The steady RANS equations for turbulent incompressible fluid flow and six turbulence closures are used in the present study. The commercial software code, FLUENT 6.3.26, was used for solving the set of governing equations using various turbulence models. Five of the used turbulence models are available directly in the code while the v2-f turbulence model was implemented via User Defined Scalars (UDS) and User Defined Functions (UDF). A series of computational analysis is performed to assess the performance of turbulence models at different grid density. The results show that the standard k-ω, SST k-ω and v2-f models clearly performed better than other models when an adverse pressure gradient was present. The RSM model shows an acceptable agreement with the velocity and turbulent kinetic energy profiles but it failed to predict the location of separation and attachment points. The standard k-ε and the low-Re k- ε delivered very poor results.
Abstract: The scroll pump belongs to the category of positive
displacement pump can be used for continuous pumping of gases at
low pressure apart from general vacuum application. The shape of
volume occupied by the gas moves and deforms continuously as the
spiral orbits. To capture flow features in such domain where mesh
deformation varies with time in a complicated manner, mesh less
solver was found to be very useful. Least Squares Kinetic Upwind
Method (LSKUM) is a kinetic theory based mesh free Euler solver
working on arbitrary distribution of points. Here upwind is enforced
in molecular level based on kinetic flux vector splitting scheme
(KFVS). In the present study we extended the LSKUM to moving
node viscous flow application. This new code LSKUM-NS-MN for
moving node viscous flow is validated for standard airfoil pitching
test case. Simulation performed for flow through scroll pump using
LSKUM-NS-MN code agrees well with the experimental pumping
speed data.
Abstract: In this paper, an analytical approach is used to study the coupled lateral-torsional vibrations of laminated composite beam. It is known that in such structures due to the fibers orientation in various layers, any lateral displacement will produce a twisting moment. This phenomenon is modeled by the bending-twisting material coupling rigidity and its main feature is the coupling of lateral and torsional vibrations. In addition to the material coupling, the effects of shear deformation and rotary inertia are taken into account in the definition of the potential and kinetic energies. Then, the governing differential equations are derived using the Hamilton-s principle and the mathematical model matches the Timoshenko beam model when neglecting the effect of bending-twisting rigidity. The equations of motion which form a system of three coupled PDEs are solved analytically to study the free vibrations of the beam in lateral and rotational modes due to the bending, as well as the torsional mode caused by twisting. The analytic solution is carried out in three steps: 1) assuming synchronous motion for the kinematic variables which are the lateral, rotational and torsional displacements, 2) solving the ensuing eigenvalue problem which contains three coupled second order ODEs and 3) imposing different boundary conditions related to combinations of simply, clamped and free end conditions. The resulting natural frequencies and mode shapes are compared with similar results in the literature and good agreement is achieved.
Abstract: Cry j 1 is a causative substance of Japanese cedar
pollinosis, and it may deteriorate by Cry j 1 invasion to a lower
respiratory tract. We observed airborne particles containing Cry j 1 by
an immunofluorescence technique using a fluorescence microscope,
and we clarified that Cry j 1 exist as aggregates of airborne fine
particles (< 1.1 μm) in the urban atmosphere. Airborne Cry j 1 may
react with air pollutants and be denature to a substance deteriorated
Japanese cedar pollinosis. Therefore, we applied a sodium dodecyl
sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to evaluate a
Cry j 1 reacted with various air pollutants by liquid phase reaction,
and calculated kinetics constants of Cry j 1 extracted from pollens
collected in various sites and airborne fine particles containing Cry j
1 by using a surface plasmon resonance (SPR) method. As a result, it
is suggested that Cry j 1 may be denatured by air pollutants during
the transportation to the urban atmosphere.
Abstract: We demonstrate the synthesis of intermediary views
within a sequence of color encoded, materials discriminating, X-ray
images that exhibit animated depth in a visual display. During the
image acquisition process, the requirement for a linear X-ray detector
array is replaced by synthetic image. Scale Invariant Feature
Transform, SIFT, in combination with material segmented morphing
is employed to produce synthetic imagery. A quantitative analysis of
the feature matching performance of the SIFT is presented along with
a comparative study of the synthetic imagery. We show that the total
number of matches produced by SIFT reduces as the angular
separation between the generating views increases. This effect is
accompanied by an increase in the total number of synthetic pixel
errors. The trends observed are obtained from 15 different luggage
items. This programme of research is in collaboration with the UK
Home Office and the US Dept. of Homeland Security.
Abstract: This paper presents an overview of the Ocean wave kinetic energy harvesting system. Energy harvesting is a concept by which energy is captured, stored, and utilized using various sources by employing interfaces, storage devices, and other units. Ocean wave energy harvesting in which the kinetic and potential energy contained in the natural oscillations of Ocean waves are converted into electric power. The kinetic energy harvesting system could be used for a number of areas. The main applications that we have discussed in this paper are to how generate the energy from Ocean wave energy (kinetic energy) to electric energy that is to eliminate the requirement for continual battery replacement.
Abstract: This paper is focused on issues of process modeling
and two model based control strategies of a fed-batch sugar
crystallization process applying the concept of artificial neural
networks (ANNs). The control objective is to force the operation into
following optimal supersaturation trajectory. It is achieved by
manipulating the feed flow rate of sugar liquor/syrup, considered as
the control input. The control task is rather challenging due to the
strong nonlinearity of the process dynamics and variations in the
crystallization kinetics. Two control alternatives are considered –
model predictive control (MPC) and feedback linearizing control
(FLC). Adequate ANN process models are first built as part of the
controller structures. MPC algorithm outperforms the FLC approach
with respect to satisfactory reference tracking and smooth control
action. However, the MPC is computationally much more involved
since it requires an online numerical optimization, while for the FLC
an analytical control solution was determined.
Abstract: Transdermal delivery of ondansetron hydrochloride (OdHCl) can prevent the problems encountered with oral ondansetron. In previously conducted studies, effect of amount of polyvinyl pyrrolidone, permeation enhancer and casting solvent on the physicochemical properties on OdHCl were investigated. It is feasible to develop ondansetron transdermal patch by using ethyl cellulose and polyvinyl pyrrolidone with dibutyl pthalate as plasticizer, however, the desired flux is not achieved. The primary aim of this study is to use dimethyl succinate (DMS) and propylene glycol that are not incorporated in previous studies to determine their effect on the physicochemical properties of an OdHCl transdermal patch using ethyl cellulose and polyvinyl pyrrolidone. This study also investigates the effect of permeation enhancer (eugenol and phosphatidylcholine) on the release of OdHCl. The results showed that propylene glycol is a more suitable plasticizer compared to DMS in the fabrication of OdHCl transdermal patch using ethyl cellulose and polyvinyl pyrrolidone as polymers. Propylene glycol containing patch has optimum drug content, thickness, moisture content and water absorption, tensile strength, and a better release profile than DMS. Eugenol and phosphatidylcholine can increase release of OdHCl from the patches. From the physicochemical result and permeation profile, a combination of 350mg of ethyl cellulose, 150mg polyvinyl pyrrolidone, 3% of total polymer weight of eugenol, and 40% of total polymer weight of propylene glycol is the most suitable formulation to develop an OdHCl patch. OdHCl release did not increase with increasing the percentage of plasticiser. DMS 4, PG 4, DMS 9, PG 9, DMS 14, and PG 14 gave better release profiles where using 300mg: 0mg, 300mg: 100mg, and 350mg: 150mg of EC: PVP. Thus, 40% of PG or DMS appeared to be the optimum amount of plasticiser when the above combination where EC: PVP was used. It was concluded from the study that a patch formulation containing 350mg EC, 150mg PVP, 40% PG and 3% eugenol is the best transdermal matrix patch compositions for the uniform and continuous release/permeation of OdHCl over an extended period. This patch design can be used for further pharmacokinetic and pharmacodynamic studies in suitable animal models.
Abstract: Four phenylurea herbicides (isoproturon, chlortoluron, diuron and linuron) were dissolved in different water matrices in order to study their chemical degradation by using UV radiation, ozone and some advanced oxidation processes (UV/H2O2, O3/H2O2, Fenton reagent and the photo- Fenton system). The waters used were: ultra-pure water, a commercial mineral water, a groundwater and a surface water taken from a reservoir. Elimination levels were established for each herbicide and for several global quality parameters, and a kinetic study was performed in order to determine basic kinetic parameters of each reaction between the target phenylureas and these oxidizing systems.
Abstract: Poly-β-hydroxybutyrate (PHB) is one of the most
famous biopolymers that has various applications in production of
biodegradable carriers. The most important strategy for enhancing
efficiency in production process and reducing the price of PHB, is the
accurate expression of kinetic model of products formation and
parameters that are effective on it, such as Dry Cell Weight (DCW)
and substrate consumption. Considering the high capabilities of
artificial neural networks in modeling and simulation of non-linear
systems such as biological and chemical industries that mainly are
multivariable systems, kinetic modeling of microbial production of
PHB that is a complex and non-linear biological process, the three
layers perceptron neural network model was used in this study.
Artificial neural network educates itself and finds the hidden laws
behind the data with mapping based on experimental data, of dry cell
weight, substrate concentration as input and PHB concentration as
output. For training the network, a series of experimental data for
PHB production from Hydrogenophaga Pseudoflava by glucose
carbon source was used. After training the network, two other
experimental data sets that have not intervened in the network
education, including dry cell concentration and substrate
concentration were applied as inputs to the network, and PHB
concentration was predicted by the network. Comparison of predicted
data by network and experimental data, indicated a high precision
predicted for both fructose and whey carbon sources. Also in present
study for better understanding of the ability of neural network in
modeling of biological processes, microbial production kinetic of
PHB by Leudeking-Piret experimental equation was modeled. The
Observed result indicated an accurate prediction of PHB
concentration by artificial neural network higher than Leudeking-
Piret model.
Abstract: Rice husk is a lignocellulosic source that can be
converted to ethanol. Three hundreds grams of rice husk was mixed
with 1 L of 0.18 N sulfuric acid solutions then was heated in an
autoclave. The reaction was expected to be at constant temperature
(isothermal), but before that temperature was achieved, reaction has
occurred. The first liquid sample was taken at temperature of 140 0C
and repeated every 5 minute interval. So the data obtained are in the
regions of non-isothermal and isothermal. It was observed that the
degradation has significant effects on the ethanol production. The
kinetic constants can be expressed by Arrhenius equation with the
frequency factors for hydrolysis and sugar degradation of 1.58 x 105
1/min and 2.29 x 108 L/mole/min, respectively, while the activation
energies are 64,350 J/mole and 76,571 J/mole. The highest ethanol
concentration from fermentation is 1.13% v/v, attained at 220 0C.
Abstract: Fuller’s earth is a fine-grained, naturally occurring substance that has a substantial ability to adsorb impurities. In the present study Fuller’s earth has been characterized and used for the removal of Pb(II) from aqueous solution. The effect of various physicochemical parameters such as pH, adsorbent dosage and shaking time on adsorption were studied. The result of the equilibrium studies showed that the solution pH was the key factor affecting the adsorption. The optimum pH for adsorption was 5. Kinetics data for the adsorption of Pb(II) was best described by pseudo-second order model. The effective diffusion co-efficient for Pb(II) adsorption was of the order of 10-8 m2/s. The adsorption data for metal adsorption can be well described by Langmuir adsorption isotherm. The maximum uptake of metal was 103.3 mg/g of adsorbent. Mass transfer analysis was also carried out for the adsorption process. The values of mass transfer coefficients obtained from the study indicate that the velocity of the adsorbate transport from bulk to the solid phase was quite fast. The mean sorption energy calculated from Dubinin-Radushkevich isotherm indicated that the metal adsorption process was chemical in nature.
Abstract: Complex assemblies of interacting proteins carry out
most of the interesting jobs in a cell, such as metabolism, DNA
synthesis, mitosis and cell division. These physiological properties
play out as a subtle molecular dance, choreographed by underlying
regulatory networks that control the activities of cyclin-dependent
kinases (CDK). The network can be modeled by a set of nonlinear
differential equations and its behavior predicted by numerical
simulation. In this paper, an innovative approach has been proposed
that uses genetic algorithms to mine a set of behavior data output by
a biological system in order to determine the kinetic parameters of
the system. In our approach, the machine learning method is
integrated with the framework of existent biological information in a
wiring diagram so that its findings are expressed in a form of system
dynamic behavior. By numerical simulations it has been illustrated
that the model is consistent with experiments and successfully shown
that such application of genetic algorithms will highly improve the
performance of mathematical model of the cell division cycle to
simulate such a complicated bio-system.
Abstract: Linear stochastic estimation and quadratic stochastic
estimation techniques were applied to estimate the entire velocity
flow-field of an open cavity with a length to depth ratio of 2. The
estimations were done through the use of instantaneous velocity
magnitude as estimators. These measurements were obtained by
Particle Image Velocimetry. The predicted flow was compared
against the original flow-field in terms of the Reynolds stresses and
turbulent kinetic energy. Quadratic stochastic estimation proved to be
more superior than linear stochastic estimation in resolving the shear
layer flow. When the velocity fluctuations were scaled up in the
quadratic estimate, both the time-averaged quantities and the
instantaneous cavity flow can be predicted to a rather accurate extent.