Analysis of a Lignocellulose Degrading Microbial Consortium to Enhance the Anaerobic Digestion of Rice Straws

Rice straw is lignocellulosic biomass which can be utilized as substrate for the biogas production. However, due to the property and composition of rice straw, it is difficult to be degraded by hydrolysis enzymes. One of the pretreatment methods that modify such properties of lignocellulosic biomass is the application of lignocellulose-degrading microbial consortia. The aim of this study is to investigate the effect of microbial consortia to enhance biogas production. To select the high efficient consortium, cellulase enzymes were extracted and their activities were analyzed. The results suggested that microbial consortium culture obtained from cattle manure is the best candidate compared to decomposed wood and horse manure. A microbial consortium isolated from cattle manure was then mixed with anaerobic sludge and used as inoculum for biogas production. The optimal conditions for biogas production were investigated using response surface methodology (RSM). The tested parameters were the ratio of amount of microbial consortium isolated and amount of anaerobic sludge (MI:AS), substrate to inoculum ratio (S:I) and temperature. Here, the value of the regression coefficient R2 = 0.7661 could be explained by the model which is high to advocate the significance of the model. The highest cumulative biogas yield was 104.6 ml/g-rice straw at optimum ratio of MI:AS, ratio of S:I, and temperature of 2.5:1, 15:1 and 44°C respectively.

Stability and Kinetic Analysis during Vermicomposting of Sewage Sludge

The present study is aimed at alteration of sewage sludge into stable compost product using vermicomposting of sewage sludge mixed with cattle manure and saw dust in five different proportions based on C/N ratios (C/N 15 (R1), 20 (R2), 25 (R3) and 30 (R4); and control (R5)) by employing an epigeic earthworm Eisenia fetida. Higher reductions in C/N ratio, CO2 evolution and OUR were observed in R4 demonstrated the compost stability. In addition, R4 proved to be best combination for the growth of the earthworms. In order to observe the optimal degradation, kinetics for degradation of organic matter in vermicomposting were quantitatively evaluated. An approach model was developed by assuming that composting process is carried out in a homogeneous way and the kinetics for decomposition reaction is represented by a Monod-type equation. The results exhibit comparable variations in the kinetic constants Km and K3 under varying parameters during vermicomposting process. Results suggested that higher R2 value in R4, enhanced suitability towards Lineweaver-Burke plot. R4 yields higher degradability coefficient (K) reveals that the occurrence of optimal nutrient balance, which not only enhanced the affinity of enzymes towards substrate but also improved its degradation process. Therefore, it can be proved that R4 provided to be the best feed combination for vermicomposting process as compared to other reactors.

Effect of Utilization of Organic and Inorganic Nitrogen Source on the Potato Shoots Dry Matter, Leaf Area Index and Plant Height, During Middle Stage of Growth

Cattle manure and mineral fertilizers are two source of Nitrogen, which can affect the growth and quantity of potato. In this research the effects of the use of cattle manure (5, 10, 15 and 20 ton ha-1), Nitrogen fertilizer (50, 100 and 150 kg N ha-1) and their interaction on potato growth were evaluated during field experiments in 2008 with the help of Randomized Complete Block (RCB) with the factorial arrangement of three experimental replications in Iran. At the 75 th day after emergence, dry weight of Shoots, leaf area index (LAI) and plant height were recorded. Results showed that, dry weight of Shoots, LAI and plant height increased linearly and very significantly in response to the application of manure and Nitrogen fertilizer. While the interaction between manure and Nitrogen fertilizer just on the LAI and plant height was significant, somehow the maximum amount of plant height( 73 cm) was obtained by using 150 kg Nitrogen + 15 tons of manure per hectare, and maximum LAI ( 5.36) was obtained by using 150 kg Nitrogen + 20 tons of manure per hectare. Also in this experiment maximum tuber yield (36.8 tons ha-1) was obtained by the utilization of 150 kg Nitrogen per hectare + 20 tons manure.

The Kinetic of Biogas Production Rate from Cattle Manure in Batch Mode

In this study, the kinetic of biogas production was studied by performing a series laboratory experiment using rumen fluid of animal ruminant as inoculums. Cattle manure as substrate was inoculated by rumen fluid to the anaerobic biodigester. Laboratory experiments using 400 ml biodigester were performed in batch operation mode. Given 100 grams of fresh cattle manure was fed to each biodigester and mixed with rumen fluid by manure : rumen weight ratio of 1:1 (MR11). The operating temperatures were varied at room temperature and 38.5 oC. The cumulative volume of biogas produced was used to measure the biodigester performance. The research showed that the rumen fluid inoculated to biodigester gave significant effect to biogas production (P

Integrated Use of Animal Manure and Inorganic Fertilizer on Growth and Yield of Vegetable Cowpea (Vigna uniquiculata)

Field experiment was conducted to investigate the combine use of animal manure and inorganic fertilizer on growth and yield performance of vegetable cowpea. The experiment was laid out in a Randomized Complete Block Design (RCBD) with seven treatments. Poultry manure, cattle manure and goat manure were evaluated with recommended level of inorganic fertilizer for vegetable cowpea. The highest crop yield was obtained by the application of poultry manure combined with the recommended level of inorganic fertilizer. The lowest yield was obtained by the application of goat manure only. In addition, the results revealed that the goat manure and cattle manure were inferior to poultry manure as a source of organic manure for vegetable cowpea cultivation. The animal manure combine with chemical fertilizer gave a higher yield when compared to the sole application of animal manure. The soil analysis showed that the nitrogen content and phosphorus content of poultry manure treated plots were higher than other treatments tested. But potassium content was higher in goat manure treated plots. The results further revealed that the poultry manure has a beneficial effect on crop growth and yield compared with other treatments. Therefore, the combined use of poultry manure with inorganic fertilizer application has been recognized as the most suitable way of ensuring high crop yield.