Abstract: Liposomal magnetofection is the most powerful nonviral method for the nucleic acid delivery into the cultured cancer cells and widely used for in vitro applications. Use of the static magnetic field condition may result in non-uniform distribution of aggregate complexes on the surface of cultured cells. To prevent this, we developed the new device which allows to concentrate aggregate complexes under dynamic magnetic field, assisting more contact of these complexes with cellular membrane and, possibly, stimulating endocytosis. Newly developed device for magnetofection under dynamic gradient magnetic field, “DynaFECTOR", was used to compare transfection efficiency of human liver hepatocellular carcinoma cell line HepG2 with that obtained by lipofection and magnetofection. The effect of two parameters on transfection efficiency, incubation time under dynamic magnetic field and rotation frequency of magnet, was estimated. Liposomal magnetofection under dynamic gradient magnetic field showed the highest transfection efficiency for HepG2 cells.
Abstract: Knowledge Discovery in Databases (KDD) has
evolved into an important and active area of research because of
theoretical challenges and practical applications associated with the
problem of discovering (or extracting) interesting and previously
unknown knowledge from very large real-world databases. Rough
Set Theory (RST) is a mathematical formalism for representing
uncertainty that can be considered an extension of the classical set
theory. It has been used in many different research areas, including
those related to inductive machine learning and reduction of
knowledge in knowledge-based systems. One important concept
related to RST is that of a rough relation. In this paper we presented
the current status of research on applying rough set theory to KDD,
which will be helpful for handle the characteristics of real-world
databases. The main aim is to show how rough set and rough set
analysis can be effectively used to extract knowledge from large
databases.
Abstract: Availability of high dimensional biological datasets such as from gene expression, proteomic, and metabolic experiments can be leveraged for the diagnosis and prognosis of diseases. Many classification methods in this area have been studied to predict disease states and separate between predefined classes such as patients with a special disease versus healthy controls. However, most of the existing research only focuses on a specific dataset. There is a lack of generic comparison between classifiers, which might provide a guideline for biologists or bioinformaticians to select the proper algorithm for new datasets. In this study, we compare the performance of popular classifiers, which are Support Vector Machine (SVM), Logistic Regression, k-Nearest Neighbor (k-NN), Naive Bayes, Decision Tree, and Random Forest based on mock datasets. We mimic common biological scenarios simulating various proportions of real discriminating biomarkers and different effect sizes thereof. The result shows that SVM performs quite stable and reaches a higher AUC compared to other methods. This may be explained due to the ability of SVM to minimize the probability of error. Moreover, Decision Tree with its good applicability for diagnosis and prognosis shows good performance in our experimental setup. Logistic Regression and Random Forest, however, strongly depend on the ratio of discriminators and perform better when having a higher number of discriminators.
Abstract: Fourty one strains of ESBL producing P.aeruginosa
which were previously isolated from burn patients in Kerman
University general hospital, Iran were subjected to PCR, RFLP and
sequencing in order to determine the type of extended spectrum β-
lactamases (ESBL), the restriction digestion pattern and possibility of
mutation among detected genes. DNA extraction was carried out by
phenol chloroform method. PCR for detection of bla genes was
performed using specific primer for each gene. Restriction Fragment
Length Polymorphism (RFLP) for ESBL genes was carried out using
EcoRI, NheI, PVUII, EcoRV, DdeI, and PstI restriction enzymes. The
PCR products were subjected to direct sequencing of both the strands
for identification of the ESBL genes.The blaCTX-M, blaVEB-1, blaPER-1,
blaGES-1, blaOXA-1, blaOXA-4 and blaOXA-10 genes were detected in the
(n=1) 2.43%, (n=41)100%, (n=28) 68.3%, (n=10) 24.4%, (n=29)
70.7%, (n=7)17.1% and (n=38) 92.7% of the ESBL producing isolates
respectively. The RFLP analysis showed that each ESBL gene has
identical pattern of digestion among the isolated strains. Sequencing
of the ESBL genes confirmed the genuinety of PCR products and
revealed no mutation in the restriction sites of the above genes. From
results of the present investigation it can be concluded that blaVEB-1
and blaCTX-M were the most and the least frequently isolated ESBL
genes among the P.aeruginosa strains isolated from burn patients. The
RFLP and sequencing analysis revealed that same clone of the bla
genes were indeed existed among the antibiotic resistant strains.
Abstract: Dengue is a mosquito-borne infection that has peaked to an alarming rate in recent decades. It can be found in tropical and sub-tropical climate. In Malaysia, dengue has been declared as one of the national health threat to the public. This study aimed to map the spatial distributions of dengue cases in the district of Hulu Langat, Selangor via a combination of Geographic Information System (GIS) and spatial statistic tools. Data related to dengue was gathered from the various government health agencies. The location of dengue cases was geocoded using a handheld GPS Juno SB Trimble. A total of 197 dengue cases occurring in 2003 were used in this study. Those data then was aggregated into sub-district level and then converted into GIS format. The study also used population or demographic data as well as the boundary of Hulu Langat. To assess the spatial distribution of dengue cases three spatial statistics method (Moran-s I, average nearest neighborhood (ANN) and kernel density estimation) were applied together with spatial analysis in the GIS environment. Those three indices were used to analyze the spatial distribution and average distance of dengue incidence and to locate the hot spot of dengue cases. The results indicated that the dengue cases was clustered (p < 0.01) when analyze using Moran-s I with z scores 5.03. The results from ANN analysis showed that the average nearest neighbor ratio is less than 1 which is 0.518755 (p < 0.0001). From this result, we can expect the dengue cases pattern in Hulu Langat district is exhibiting a cluster pattern. The z-score for dengue incidence within the district is -13.0525 (p < 0.0001). It was also found that the significant spatial autocorrelation of dengue incidences occurs at an average distance of 380.81 meters (p < 0.0001). Several locations especially residential area also had been identified as the hot spots of dengue cases in the district.
Abstract: Weblog is an Internet tool that is believed to possess
great potential to facilitate learning in education. This study wants to
know if weblog can be used to promote students- critical thinking. It
used a group of secondary two students from a Singapore school to
write weblogs as a means of substitution for their traditional
handwritten assignments. The topics for the weblogging are taken
from History syllabus but modified to suit the purpose of this study.
Weblogs from the students were collected and analysed using a
known coding system for measuring critical thinking. Results show
that the topic for blogging is crucial in determining the types of
critical thinking employed by the students. Students are seen to
display critical thinking traits in the areas of information sourcing,
linking information to arguments and viewpoints justification.
Students- criticalness is more profound when the information for
writing a topic is readily available. Otherwise, they tend to be less
critical and subjective. The study also found that students lack the
ability to source for external information suggesting that students
may need to be taught information literacy in order to widen their use
of critical thinking skills.
Abstract: Routing places an important role in determining the
quality of service in wireless networks. The routing methods adopted
in wireless networks have many drawbacks. This paper aims to
review the current routing methods used in wireless networks. This
paper proposes an innovative solution to overcome the problems in
routing. This solution is aimed at improving the Quality of Service.
This solution is different from others as it involves the resuage of the
part of the virtual circuits. This improvement in quality of service is
important especially in propagation of multimedia applications like
video, animations etc. So it is the dire need to propose a new solution
to improve the quality of service in ATM wireless networks for
multimedia applications especially during this era of multimedia
based applications.
Abstract: In this paper, the application of GRNN in
modeling of SOFC fuel cells were studied. The parameters
are of interested as voltage and power value and the current
changes are investigated. In addition, the comparison between
GRNN neural network application and conventional method
was made. The error value showed the superlative results.
Abstract: The objective of the present communication is to
develop new genuine exponentiated mean codeword lengths and to
study deeply the problem of correspondence between well known
measures of entropy and mean codeword lengths. With the help of
some standard measures of entropy, we have illustrated such a
correspondence. In literature, we usually come across many
inequalities which are frequently used in information theory.
Keeping this idea in mind, we have developed such inequalities via
coding theory approach.
Abstract: With the exponential progress of technological
development comes a strong sense that events are moving too quickly
for our schools and that teachers may be losing control of them in the
process. This paper examines the impact of e-learning and e-teaching
in universities, from both the student and teacher perspective. In
particular, it is shown that e-teachers should focus not only on the
technical capacities and functions of IT materials and activities, but
must attempt to more fully understand how their e-learners perceive
the learning environment. From the e-learner perspective, this paper
indicates that simply having IT tools available does not automatically
translate into all students becoming effective learners. More
evidence-based evaluative research is needed to allow e-learning and
e-teaching to reach full potential.
Abstract: According as the Architecture, Engineering and Construction (AEC) Industry projects have grown more complex and larger, the number of utilization of BIM for 3D design and simulation is increasing significantly. Therefore, typical applications of BIM such as clash detection and alternative measures based on 3-dimenstional planning are expanded to process management, cost and quantity management, structural analysis, check for regulation, and various domains for virtual design and construction. Presently, commercial BIM software is operated on single-user environment, so initial cost is so high and the investment may be wasted frequently. Cloud computing that is a next-generation internet technology enables simple internet devices (such as PC, Tablet, Smart phone etc) to use services and resources of BIM software. In this paper, we suggested developing method of the BIM software based on cloud computing environment in order to expand utilization of BIM and reduce cost of BIM software. First, for the benchmarking, we surveyed successful case of BIM and cloud computing. And we analyzed needs and opportunities of BIM and cloud computing in AEC Industry. Finally, we suggested main functions of BIM software based on cloud computing environment and developed a simple prototype of cloud computing BIM software for basic BIM model viewing.
Abstract: System-level design based on high-level abstractions
is becoming increasingly important in hardware and embedded
system design. This paper analyzes meta-design techniques oriented
at developing meta-programs and meta-models for well-understood
domains. Meta-design techniques include meta-programming and
meta-modeling. At the programming level of design process, metadesign
means developing generic components that are usable in a
wider context of application than original domain components. At the
modeling level, meta-design means developing design patterns that
describe general solutions to the common recurring design problems,
and meta-models that describe the relationship between different
types of design models and abstractions. The paper describes and
evaluates the implementation of meta-design in hardware design
domain using object-oriented and meta-programming techniques.
The presented ideas are illustrated with a case study.
Abstract: The counting and analysis of blood cells allows the
evaluation and diagnosis of a vast number of diseases. In particular,
the analysis of white blood cells (WBCs) is a topic of great interest to
hematologists. Nowadays the morphological analysis of blood cells is
performed manually by skilled operators. This involves numerous
drawbacks, such as slowness of the analysis and a nonstandard
accuracy, dependent on the operator skills. In literature there are only
few examples of automated systems in order to analyze the white
blood cells, most of which only partial. This paper presents a
complete and fully automatic method for white blood cells
identification from microscopic images. The proposed method firstly
individuates white blood cells from which, subsequently, nucleus and
cytoplasm are extracted. The whole work has been developed using
MATLAB environment, in particular the Image Processing Toolbox.
Abstract: Biodiesel as an alternative fuel for diesel engines has been developed for some three decades now. While it is gaining wide acceptance in Europe, USA and some parts of Asia, the same cannot be said of Africa. With more than 35 countries in the continent depending on imported crude oil, it is necessary to look for alternative fuels which can be produced from resources available locally within any country. Hence this study presents performance of single cylinder diesel engine using blends of shea butter biodiesel. Shea butter was transformed into biodiesel by transesterification process. Tests are conducted to compare the biodiesel with baseline diesel fuel in terms of engine performance and exhaust emission characteristics. The results obtained showed that the addition of biodiesel to diesel fuel decreases the brake thermal efficiency (BTE) and increases the brake specific fuel consumption (BSFC). These results are expected due to the lower energy content of biodiesel fuel. On the other hand while the NOx emissions increased with increase in biodiesel content in the fuel blends, the emissions of carbon monoxide (CO), un-burnt hydrocarbon (UHC) and smoke opacity decreased. The engine performance which indicates that the biodiesel has properties and characteristics similar to diesel fuel and the reductions in exhaust emissions make shea butter biodiesel a viable additive or substitute to diesel fuel.
Abstract: The recent development of humanoid robots has led robot designers to imagine a great variety of anthropomorphic forms for human-like machine. Which form is the best ? We try to answer this question from a double meaning of the anthropomorphism : a positive anthropomorphism corresponing to the realization of an effective anthropomorphic form object and a negative one corresponding to our natural tendency in certain circumstances to give human attributes to non-human beings. We postulate that any humanoid robot is concerned by both these two anthropomorphism kinds. We propose to use gestalt theory and Heider-s balance theory in order to analyze how negative anthropomorphism can influence our perception of human-like robots. From our theoretical approach we conclude that an “even shape" as defined by gestalt theory is not a sufficient condition for a good integration of future humanoid robots into a human community. Aesthetic perception of the robot cannot be splitted from a social perception : a humanoid robot, any how the efforts made for improving its appearance, could be rejected if it is devoted to a task with too high affective implications.
Abstract: The purpose of this study was to develop and examine a
Teaching Commitment Scale of Health and Physical Education
(TCS-HPE) for Taiwanese elementary school teachers. First of all,
based on teaching commitment related theory and literatures to
develop a original scale with 40 items, later both stratified random
sampling and cluster sampling were used to sample participants.
During the first stage, 300 teachers were sampled and 251 valid scales
(83.7%) returned. Later, the data was analyzed by exploratory factor
analysis to obtain 74.30% of total variance for the construct validity.
The Cronbach-s alpha coefficient of sum scale reliability was 0.94, and
subscale coefficients were between 0.80 and 0.96. In the second stage,
400 teachers were sampled and 318 valid scales (79.5%) returned.
Finally, this study used confirmatory factor analysis to test validity and
reliability of TCS-HPE. The result showed that the fit indexes reached
acceptable criteria(¤ç2
(246 ) =557.64 , p
Abstract: In modern distributed software systems, the issue of communication among composing parts represents a critical point, but the idea of extending conventional programming languages with general purpose communication constructs seems difficult to realize. As a consequence, there is a (growing) gap between the abstraction level required by distributed applications and the concepts provided by platforms that enable communication. This work intends to discuss how the Model Driven Software Development approach can be considered as a mature technology to generate in automatic way the schematic part of applications related to communication, by providing at the same time high level specialized languages useful in all the phases of software production. To achieve the goal, a stack of languages (meta-meta¬models) has been introduced in order to describe – at different levels of abstraction – the collaborative behavior of generic entities in terms of communication actions related to a taxonomy of messages. Finally, the generation of platforms for communication is viewed as a form of specification of language semantics, that provides executable models of applications together with model-checking supports and effective runtime environments.
Abstract: During this day a considerable amount of Leachate is produced with high amounts of organic material and nutrients needed plants. This study has done in order to scrutinize the effect of Leachate compost on the pH, EC and organic matter percentage in the form of statistical Factorial plan through randomizing block design with three main and two minor treatments and also three replications during three six month periods. Major treatments include N: Irrigation with the region-s well water as a control, I: Frequent irrigation with well water and Leachate, C: Mixing Leachate and water well (25 percent leachate + 75 percent ordinary well water) and secondary treatments, include DI: surface drip irrigation and SDI: sub surface drip irrigation. Results of this study indicated significant differences between treatments and also there were mixing up with the control treatment in the reduction of pH, increasing soluble salts and also increasing the organic matter percentage. This increase is proportional to the amount of added Leachate and in the treatment also proportional to higher mixture of frequent treatment. Therefore, since creating an acidic pH increases the ability to absorb some nutrient elements such as phosphorus, iron, zinc, copper and manganese are increased and the other hand, organic materials also improve many physical and chemical properties of soil are used in Leachate trash Consider health issues as refined in the green belts around cities as a liquid fertilizer recommended.
Abstract: The financial crisis has decreased the opportunities of
small businesses to acquire financing through conventional financial
actors, such as commercial banks. This credit constraint is partly the
reason for the emergence of new alternatives of financing, in addition
to the spreading opportunities for communication and secure
financial transfer through Internet. One of the most interesting venues
for finance is termed “crowdfunding". As the term suggests
crowdfunding is an appeal to prospective customers and investors to
form a crowd that will finance projects that otherwise would find it
hard to generate support through the most common financial actors.
Crowdfunding is in this paper divided into different models; the
threshold model, the microfinance model, the micro loan model and
the equity model. All these models add to the financial possibilities of
emerging entrepreneurs.
Abstract: The purpose of this research was to investigate Thai Muslims’ way of life through the way their clothes. The data of this qualitative research were collected from related documents and research reports, ancient cloths and clothing, and in-depth interviews with clothes owners and weavers.
The research found that in the 18th century Thai Muslims in the three southern border provinces used many types of clothing in their life. At home women wore plain clothes. They used checked cloths to cover the upper part of their body from the breasts down to the waist. When going out, they used Lima cloth and So Kae with a piece of Pla-nging cloth as a head scarf. For men, they wore a checked sarong as a lower garment, and wore no upper garment. However, when going out, they wore Puyo Potong. In addition, Thai Muslims used cloths in various religious rites, namely, the rite of placing a baby in a cradle, the Masoyawi rite, the Nikah rite, and the burial rite. These types of cloths were related to the way of life of Thai Muslims from birth to death. They reflected the race, gender, age, social status, values, and beliefs in traditions that have been inherited.
Practical Implication: Woven in these cloths are the lost local wisdom, and therefore, aesthetics on the cloths are like mirrors reflecting the background of people in this region that is fading away. These cloths are pages of a local history book that is of importance and value worth for preservation and publicity so that they are treasured. Government organizations can expand and materialize the knowledge received from the study in accordance with government policy in supporting the One Tambon, One Product project.