Abstract: Enterococci are important inhabitants of the animal
intestine and are widely used in probiotic products. A probiotic strain
is expected to possess several desirable properties in order to exert
beneficial effects. Therefore, the objective of this study was to
isolate, characterize and identify Enterococcus sp. from chicken cecal
and fecal samples to determine potential probiotic properties.
Enterococci were isolated from chicken ceca and feces of thirty three
clinically healthy chickens from a local farm. In vitro studies were
performed to assess antibacterial activity of the isolated LAB (using
agar well diffusion and cell free supernatant broth technique against
Salmonella enterica serotype Enteritidis), survival in acidic
conditions, resistance to bile salts, and their survival during simulated
gastric juice conditions at pH 2.5. Isolates were identified by
biochemical carbohydrate fermentation patterns using an API 50
CHL kit and API ZYM kits and by sequenced 16S rDNA. An isolate
belonging to E. faecium species exhibited inhibitory effect against S.
enteritidis. This isolate producing a clear zone as large as 10.30 mm
or greater and was able to grow in the coculture medium and at the
same time, inhibited the growth S. enteritidis. In addition, E. faecium
exhibited significant resistance under highly acidic conditions at pH
2.5 for 8 h and survived well in bile salt at 0.2% for 24 h and showing
ability to survive in the presence of simulated gastric juice at pH 2.5.
Based on these results, E. faecium isolate fulfills some of the criteria
to be considered as a probiotic strain and therefore, could be used as a
feed additive with good potential for controlling S. Enteritidis in
chickens. However, in vivo studies are needed to determine the safety
of the strain.
Abstract: “Dengue" is an African word meaning “bone
breaking" because it causes severe joint and muscle pain that feels
like bones are breaking. It is an infectious disease mainly transmitted
by female mosquito, Aedes aegypti, and causes four serotypes of
dengue viruses. In recent years, a dramatic increase in the dengue
fever confirmed cases around the equator-s belt has been reported.
Several conventional indices have been designed so far to monitor the
transmitting vector populations known as House Index (HI),
Container Index (CI), Breteau Index (BI). However, none of them
describes the adult mosquito population size which is important to
direct and guide comprehensive control strategy operations since
number of infected people has a direct relationship with the vector
density. Therefore, it is crucial to know the population size of the
transmitting vector in order to design a suitable and effective control
program. In this context, a study is carried out to report a new
statistical index, ABURAS Index, using Poisson distribution based
on the collection of vector population in Jeddah Governorate, Saudi Arabia.
Abstract: Dengue, a disease found in most tropical and
subtropical areas of the world. It has become the most common
arboviral disease of humans. This disease is caused by any of four
serotypes of dengue virus (DEN1-DEN4). In many endemic
countries, the average age of getting dengue infection is shifting
upwards, dengue in pregnancy and infancy are likely to be
encountered more frequently. The dynamics of the disease is studied
by a compartmental model involving ordinary differential equations
for the pregnant, infant human and the vector populations. The
stability of each equilibrium point is given. The epidemic dynamic is
discussed. Moreover, the numerical results are shown for difference
values of dengue antibody.
Abstract: Dengue virus is transmitted from person to person
through the biting of infected Aedes Aegypti mosquitoes. DEN-1,
DEN-2, DEN-3 and DEN-4 are four serotypes of this virus. Infection
with one of these four serotypes apparently produces permanent
immunity to it, but only temporary cross immunity to the others. The
length of time during incubation of dengue virus in human and
mosquito are considered in this study. The dengue patients are
classified into infected and infectious classes. The infectious human
can transmit dengue virus to susceptible mosquitoes but infected
human can not. The transmission model of this disease is formulated.
The human population is divided into susceptible, infected, infectious
and recovered classes. The mosquito population is separated into
susceptible, infected and infectious classes. Only infectious
mosquitoes can transmit dengue virus to the susceptible human. We
analyze this model by using dynamical analysis method. The
threshold condition is discussed to reduce the outbreak of this
disease.
Abstract: Foodborne Salmonella infections have become a
major problem world wide. Salmonellosis transmitted from fish are
quite common. Established quality control measures exist for export
oriented fish, none exists for fish consumed locally. This study aimed
at characterization of Salmonella isolated from Nile tilapia . The
study was carried out in selected beaches along L. Victoria in
Western Kenya between March and June 2007. One hundred and
twenty fish specimens were collected. Salmonella isolates were
confirmed using serotyping, biochemical testing in addition to malic
acid dehydrogenase (mdh) and fliC gene sequencing. Twenty
Salmonella isolates were confirmed by mdh gene sequencing. Nine
(9) were S. enterica serotype typhimurium, four (4) were S. enterica
Serotype, enteritidis and seven (7) were S. enterica serotype typhi.
Nile tilapia have a role in transmission of Salmonellosis in the study
area, poor sanitation was a major cause of pollution at the beach
inshore waters.