Abstract: Bean common mosaic necrosis virus (BCMNV) is a
potyvirus with a worldwide distribution. This virus causes serious
economic losses in Iran in many leguminoses. During 20008,
samples were collected from soybeans fields in Tehran Province.
Four isolates (S1, S2 and S3) were inoculated on 15 species of
Cucurbitaceae, Chenopodiaceae, Solanacae and Leguminosae.
Chenopodium quinoa and C. amaranticolor.
Did not developed any symptoms.all isolates caused mosaic
symptoms on Phaseolus vulgaris cv. Red Kidney and P. vulgaris cv.
Bountiful. The molecular weights of coat protein using SDS-PAGE
and western blotting were estimated at 33 kDa. Reverse transcription
polymerase chain reaction (RT-PCR) was performed using one
primer pairs designed by L. XU et al. An approximately 920 bp
fragment was amplified with a specific primer.
Abstract: The anti-lock braking systems installed on vehicles
for safe and effective braking, are high-order nonlinear and timevariant.
Using fuzzy logic controllers increase efficiency of such
systems, but impose a high computational complexity as well. The
main concept introduced by this paper is reducing computational
complexity of fuzzy controllers by deploying problem-solution data
structure. Unlike conventional methods that are based on
calculations, this approach is based on data oriented modeling.
Abstract: This paper is on the general discussion of memory consistency model like Strict Consistency, Sequential Consistency, Processor Consistency, Weak Consistency etc. Then the techniques for implementing distributed shared memory Systems and Synchronization Primitives in Software Distributed Shared Memory Systems are discussed. The analysis involves the performance measurement of the protocol concerned that is Multiple Writer Protocol. Each protocol has pros and cons. So, the problems that are associated with each protocol is discussed and other related things are explored.
Abstract: Synthetic juice clarification was done through spiral
wound ultrafiltration (UF) membrane module. Synthetic juice was
clarified at two different operating conditions, such as, with and
without permeates recycle at turbulent flow regime. The performance
of spiral wound ultrafiltration membrane was analyzed during
clarification of synthetic juice. Synthetic juice was the mixture of
deionized water, sucrose and pectin molecule. The operating
conditions are: feed flowrate of 10 lpm, pressure drop of 413.7 kPa
and Reynolds no of 5000. Permeate sample was analyzed in terms of
volume reduction factor (VRF), viscosity (Pa.s), ⁰Brix, TDS (mg/l),
electrical conductivity (μS) and turbidity (NTU). It was observe that
the permeate flux declined with operating time for both conditions of
with and without permeate recycle due to increase of concentration
polarization and increase of gel layer on membrane surface. For
without permeate recycle, the membrane fouling rate was faster
compared to with permeate recycle. For without permeate recycle,
the VRF rose up to 5 and for with recycle permeate the VRF is 1.9.
The VRF is higher due to adsorption of solute (pectin) molecule on
membrane surface and resulting permeateflux declined with VRF.
With permeate recycle, quality was within acceptable limit. Fouled
membrane was cleaned by applying different processes (e.g.,
deionized water, SDS and EDTA solution). Membrane cleaning was
analyzed in terms of permeability recovery.
Abstract: This work relates the development of an optical fiber
(OF) sensor for the detection and quantification of single walled
carbon nanotubes in aqueous solutions. The developed OF displays a
compact design, it requires less expensive materials and equipment
as well as low volume of sample (0.2 mL). This methodology was
also validated by the comparison of its analytical performance with
that of a standard methodology based on ultraviolet-visible
spectroscopy. The developed OF sensor follows the general SDS
calibration proposed for OF sensors as a more suitable calibration
fitting compared with classical calibrations.
Abstract: Von Willebrand-s disease is the most common
inherited bleeding disorder in humans, it
caused by qualitative abnormalities of the von Willebrand factor
(vWF). Our objective is to determine the prevalence of this disease at
part of the Algerian population in the East and the South by a
biological diagnosis based on specific biological tests (automated
platelet count, the bleeding time (TS), the time of cephalin + activator
(TCA), measure of the prothrombin rate (TP), vWF rate and factor
VIII rate, Molecular electrophoresis of vWF multimers in agarose gel
in the presence of SDS). Four patients of type III or severe
Willebrand-s disease were found on 200 suspect cases. All cases are
showed a deficit in vWF rate (< 5%), and factor VIII (P
Abstract: Herein, we report the different types of surface morphology due to the interaction between the pure protein Insulin (INS) and catanionic surfactant mixture of Sodium Dodecyl Sulfate (SDS) and Cetyl Trimethyl Ammonium Bromide (CTAB) at air/water interface obtained by the Langmuir-Blodgett (LB) technique. We characterized the aggregations by Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR) in LB films. We found that the INS adsorption increased in presence of catanionic surfactant at air/water interface. The presence of small amount of surfactant induces two-stage growth kinetics due to the pure protein absorption and protein-catanionic surface micelle interaction. The protein remains in native state in presence of small amount of surfactant mixture. Smaller amount of surfactant mixture with INS is producing surface micelle type structure. This may be considered for drug delivery system. On the other hand, INS becomes unfolded and fibrillated in presence of higher amount of surfactant mixture. In both the cases, the protein was successfully immobilized on a glass substrate by the LB technique. These results may find applications in the fundamental science of the physical chemistry of surfactant systems, as well as in the preparation of drug-delivery system.
Abstract: Ethanol is generally used as a therapeutic reagent against Hepatocellular carcinoma (HCC or hepatoma) worldwide, as it can induce Hepatocellular carcinoma cell apoptosis at low concentration through a multifactorial process regulated by several unknown proteins. This paper provides a simple and available proteomic strategy for exploring differentially expressed proteins in the apoptotic pathway. The appropriate concentrations of ethanol required to induce HepG2 cell apoptosis were first assessed by MTT assay, Gisma and fluorescence staining. Next, the central proteins involved in the apoptosis pathway processs were determined using 2D-PAGE, SDS-PAGE, and bio-software analysis. Finally the downregulation of two proteins, AFP and survivin, were determined by immunocytochemistry and reverse transcriptase PCR (RT-PCR) technology. The simple, useful method demonstrated here provides a new approach to proteomic analysis in key bio-regulating process including proliferation, differentiation, apoptosis, immunity and metastasis.
Abstract: Characterization and evaluation of the activity of Vespa basalis DPP-IV, which expressed in Spodoptera frugiperda 21 cells. The expression of rDPP-IV was confirmed by SDS–PAGE, Western blot analyses, LC-MS/MS and measurement of its peptidase specificity. One-step purification by Ni-NTA affinity chromatography and the total amount of rDPP-IV recovered was approximately 6.4mg per liter from infected culture medium; an equivalent amount would be produced by 1x109 infected Sf21 insect cells. Through the affinity purification led to highly stable rDPP-IV enzyme was recovered and with significant peptidase activity. The rDPP-IV exhibited classical Michaelis–Menten kinetics, with kcat/Km in the range of 10-500 mM-1×S-1 for the five synthetic substrates and optimum substrate is Ala-Pro-pNA. As expected in inhibition assay, the enzymatic activity of rDPP-IV was significantly reduced by 80 or 60% in the presence of sitagliptin (a DPP-IV inhibitor) or PMSF (a serine protease inhibitor), but was not apparently affected by iodoacetamide (a cysteine protease inhibitor).
Abstract: In this study, solid phase micro-extraction (SPME)
was optimized to improve the sensitivity and accuracy in
formaldehyde determination for plywood panels. Further work has
been carried out to compare the newly developed technique with
existing method which reacts formaldehyde collected in desiccators
with acetyl acetone reagent (DC-AA). In SPME, formaldehyde was
first derivatized with O-(2,3,4,5,6 pentafluorobenzyl)-hydroxylamine
hydrochloride (PFBHA) and analysis was then performed by gas
chromatography in combination with mass spectrometry (GC-MS).
SPME data subjected to various wood species gave satisfactory
results, with relative standard deviations (RSDs) obtained in the
range of 3.1-10.3%. It was also well correlated with DC values,
giving a correlation coefficient, RSQ, of 0.959. The quantitative
analysis of formaldehyde by SPME was an alternative in wood
industry with great potential
Abstract: Soil washing process with a surfactant solution is a potential technology for the rapid removal of hydrophobic organic compound (HOC) from soil. However, large amount of washed water would be produced during operation and this should be treated effectively by proper methods. The soil washed water for complex contaminated site with HOC and heavy metals might contain high amount of pollutants such as HOC and heavy metals as well as used surfactant. The heavy metals in the soil washed water have toxic effects on microbial activities thus these should be removed from the washed water before proceeding to a biological waste-water treatment system. Moreover, the used surfactant solutions are necessary to be recovered for reducing the soil washing operation cost. In order to simultaneously remove the heavy metals and HOC from soil-washed water, activated carbon (AC) was used in the present study. In an anionic-nonionic surfactant mixed solution, the Cd(II) and phenanthrene (PHE) were effectively removed by adsorption on activated carbon. The removal efficiency for Cd(II) was increased from 0.027 mmol-Cd/g-AC to 0.142 mmol-Cd/g-AC as the mole ratio of SDS increased in the presence of PHE. The adsorptive capacity of PHE was also increased according to the SDS mole ratio due to the decrement of molar solubilization ratios (MSR) for PHE in an anionic-nonionic surfactant mixture. The simultaneous adsorption of HOC and cationic heavy metals using activated carbon could be a useful method for surfactant recovery and the reduction of heavy metal toxicity in a surfactant-enhanced soil washing process.
Abstract: The spores of entomopathogenic fungi, Beauveria bassiana was evaluated for their compatibility with four surfactants; SDS (sodium dodyl sulphate) and CABS-65 (calcium alkyl benzene sulphonate), Tween 20 (polyethylene sorbitan monolaureate) and Tween 80 (polyoxyethylene sorbitan monoleate) at six different concentrations (0.1%, 0.5%, 1%, 2.5%, 5% and 10%). Incubated spores showed decrease in concentrations due to conversion of spores to hyphae. The maximum germination recorded in 72 h incubated spores varied with surfactant concentration at 49-68% (SDS), 39- 53% (CABS), 78-92% (Tween 80) and 80-92% (Tween 20), while the optimal surfactant concentration for spore germination was found to be 2.5-5%. The surfactant effect on spores was more pronounced with SDS and CABS-65, where significant deterioration and loss in viability of the incubated spores was observed. The effect of Tween 20 and Tween 80 were comparatively less inhibiting. The results of the study would help in surfactant selection for B. bassiana emulsion preparation.
Abstract: Proteins levels produced by bacteria may be increased
in stressful surroundings, such as in the presence of antibiotics. It
appears that many antimicrobial agents or antibiotics, when used at
low concentrations, have in common the ability to activate or repress
gene transcription, which is distinct from their inhibitory effect.
There have been comparatively few studies on the potential of
antibiotics or natural compounds in nature as a specific chemical
signal that can trigger a variety of biological functions. Therefore,
this study was focusing on the effect of essential oils from
Cymbopogon flexuosus and C. nardus in regulating proteins
production by Bacillus subtilis ATCC 21332. The Minimum
Inhibition Concentrations (MICs) of both essential oils on B. subtilis
were determined by using microdilution assay, resulting 0.2% and
1.56% for each C. flexuosus and C. nardus subsequently. The
bacteria were further exposed to each essential oils at concentration
of 0.01XMIC for 2 days. The proteins were then isolated and
analyzed by sodium dodecyl sulfate polyacrylamide gel
electrophoresis (SDS-PAGE). Protein profile showed that a band
with approximate size of 250 kD was appeared for the treated
bacteria with essential oils. Thus, Bacillus subtilis ATCC 21332 in
stressful condition with the presence of essential oils at low
concentration could induce the protein production.
Abstract: Artemia is one of the most conspicuous invertebrates
associated with aquaculture. It can be considered as a model
organism, offering numerous advantages for comprehensive and
multidisciplinary studies using morphologic or molecular methods.
Since DNA extraction is an important step of any molecular
experiment, a new and a rapid method of DNA extraction from adult
Artemia was described in this study. Besides, the efficiency of this
technique was compared with two widely used alternative techniques,
namely Chelex® 100 resin and SDS-chloroform methods. Data
analysis revealed that the new method is the easiest and the most cost
effective method among the other methods which allows a quick and
efficient extraction of DNA from the adult animal.
Abstract: This paper present a new way to find the aerodynamic
characteristic equation of missile for the numerical trajectories
prediction more accurate. The goal is to obtain the polynomial
equation based on two missile characteristic parameters, angle of
attack (α ) and flight speed (ν ). First, the understudied missile is
modeled and used for flow computational model to compute
aerodynamic force and moment. Assume that performance range of
understudied missile where range -10< α
Abstract: Clean air in subway station is important to passengers. The Platform Screen Doors (PSDs) can improve indoor air quality in the subway station; however the air quality in the subway tunnel is degraded. The subway tunnel has high CO2 concentration and indoor particulate matter (PM) value. The Indoor Air Quality (IAQ) level in subway environment degrades by increasing the frequency of the train operation and the number of the train. The ventilation systems of the subway tunnel need improvements to have better air-quality. Numerical analyses might be effective tools to analyze the performance of subway twin-track tunnel ventilation systems. An existing subway twin-track tunnel in the metropolitan Seoul subway system is chosen for the numerical simulations. The ANSYS CFX software is used for unsteady computations of the airflow inside the twin-track tunnel when the train moves. The airflow inside the tunnel is simulated when one train runs and two trains run at the same time in the tunnel. The piston-effect inside the tunnel is analyzed when all shafts function as the natural ventilation shaft. The supplied air through the shafts is mixed with the pollutant air in the tunnel. The pollutant air is exhausted by the mechanical ventilation shafts. The supplied and discharged airs are balanced when only one train runs in the twin-track tunnel. The pollutant air in the tunnel is high when two trains run simultaneously in opposite direction and all shafts functioned as the natural shaft cases when there are no electrical power supplies in the shafts. The remained pollutant air inside the tunnel enters into the station platform when the doors are opened.
Abstract: The Platform Screen Doors improve Indoor Air Quality
(IAQ) in the subway station; however, and the air quality is degraded
in the subway tunnel. CO2 concentration and indoor particulate matter
value are high in the tunnel. The IAQ level in subway tunnel degrades
by increasing the train movements. Air-curtain installation reduces
dusts, particles and moving toxic smokes and permits traffic by
generating virtual wall. The ventilation systems of the subway tunnel
need improvements to have better air-quality. Numerical analyses
might be effective tools analyze the flowfield inside the air-curtain
installed subway tunnel. The ANSYS CFX software is used for steady
computations of the airflow inside the tunnel. The single-track subway
tunnel has the natural shaft, the mechanical shaft, and the PSDs
installed stations. The height and width of the tunnel are 6.0 m and 4.0
m respectively. The tunnel is 400 m long and the air-curtain is installed
at the top of the tunnel. The thickness and the width of the air-curtain
are 0.08 m and 4 m respectively. The velocity of the air-curtain
changes between 20 - 30 m/s. Three cases are analyzed depending on
the installing location of the air-curtain. The discharged-air through
the natural shafts increases as the velocity of the air-curtain increases
when the air-curtain is installed between the mechanical and the
natural shafts. The pollutant-air is exhausted by the mechanical and the
natural shafts and remained air is pushed toward tunnel end. The
discharged-air through the natural shaft is low when the air-curtain
installed before the natural shaft. The mass flow rate decreases in the
tunnel after the mechanical shaft as the air-curtain velocity increases.
The computational results of the air-curtain installed tunnel become
basis for the optimum design study. The air-curtain installing location
is chosen between the mechanical and the natural shafts. The velocity
of the air-curtain is fixed as 25 m/s. The thickness and the blowing
angles of the air-curtain are the design variables for the optimum
design study. The object function of the design optimization is
maximizing the discharged air through the natural shaft.
Abstract: Multi-residue analysis method for penicillins was
developed and validated in bovine muscle, chicken, milk, and flatfish.
Detection was based on liquid chromatography tandem mass
spectrometry (LC/MS/MS). The developed method was validated for
specificity, precision, recovery, and linearity. The analytes were
extracted with 80% acetonitrile and clean-up by a single
reversed-phase solid-phase extraction step. Six penicillins presented
recoveries higher than 76% with the exception of Amoxicillin
(59.7%). Relative standard deviations (RSDs) were not more than
10%. LOQs values ranged from 0.1 and to 4.5 ug/kg. The method was
applied to 128 real samples. Benzylpenicillin was detected in 15
samples and Cloxacillin was detected in 7 samples. Oxacillin was
detected in 2 samples. But the detected levels were under the MRL
levels for penicillins in samples.
Abstract: Lectins have a good scope in current clinical
microbiology research. In the present study evaluated the
antimicrobial activities of a D-galactose binding lectin (PnL) was
purified from the annelid, Perinereis nuntia (polychaeta) by affinity
chromatography. The molecular mass of the lectin was determined to
be 32 kDa as a single polypeptide by SDS-PAGE under both reducing
and non-reducing conditions. The hemagglutinating activity of the
PnL showed against trypsinized and glutaraldehyde-fixed human
erythrocytes was specifically inhibited by D-Gal, GalNAc,
Galβ1-4Glc and Galα1-6Glc. PnL was evaluated for in vitro
antibacterial screening studies against 11 gram-positive and
gram-negative microorganisms. From the screening results, it was
revealed that PnL exhibited significant antibacterial activity against
gram-positive bacteria. Bacillus megaterium showed the highest
growth inhibition by the lectin (250 μg/disc). However, PnL did not
inhibit the growth of gram-negative bacteria such as Vibrio cholerae
and Pseudomonas sp. PnL was also examined for in vitro antifungal
activity against six fungal phytopathogens. PnL (100 μg/mL) inhibited
the mycelial growth of Alternaria alternata (24.4%). These results
indicate that future findings of lectin applications obtained from
annelids may be of importance to life sciences.
Abstract: We fabricated multi-walled carbon nanotube (MCNT)
emitters by an electroporetic deposition (EPD) method using a
MCNT-sodium dodecyl sulfate (SDS) suspension. MCNT films were
prepared on graphite tip using EPD. We observe field emission
properties of MCNT film after heat treatment. Consequently, The
MCNT film on graphite tip exhibit good electron emission current.