Antibiotic Resistance Profile of Bacterial Isolates from Animal Farming Aquatic Environments and Meats in a Peri-Urban Community in South Korea

The increasing usage of antibiotics in the animal farming industry is an emerging worldwide problem contributing to the development of antibiotic resistance. The purpose of this work was to investigate the prevalence and antibiotic resistance profile of bacterial isolates collected from aquatic environments and meats in a peri-urban community in Daejeon, Korea. In an antibacterial susceptibility test, the bacterial isolates showed a high incidence of resistance (~ 26.04 %) to cefazolin, tetracycline, gentamycin, norfloxacin, erythromycin and vancomycin. The results from a test for multiple antibiotic resistance indicated that the isolates were displaying an approximately 5-fold increase in the incidence of multiple antibiotic resistance to combinations of two different antibiotics compared to combinations of three or more antibiotics. Most of the isolates showed multi-antibiotic resistance, and the resistance patterns were similar among the sampling groups. Sequencing data analysis of 16S rRNA showed that most of the resistant isolates appeared to be dominated by the classes Betaproteobacteria and Gammaproteobacteria in the phylum Proteobacteria.

Stress Analysis of Adhesively Bonded Double- Lap Joints Subjected to Combined Loading

Adhesively bonded joints are preferred over the conventional methods of joining such as riveting, welding, bolting and soldering. Some of the main advantages of adhesive joints compared to conventional joints are the ability to join dissimilar materials and damage-sensitive materials, better stress distribution, weight reduction, fabrication of complicated shapes, excellent thermal and insulation properties, vibration response and enhanced damping control, smoother aerodynamic surfaces and an improvement in corrosion and fatigue resistance. This paper presents the behavior of adhesively bonded joints subjected to combined thermal loadings, using the numerical methods. The joint configuration considers aluminum as central adherend with six different outer adherends including aluminum, steel, titanium, boronepoxy, unidirectional graphite-epoxy and cross-ply graphite-epoxy and epoxy-based adhesives. Free expansion of the joint in x direction was permitted and stresses in adhesive layer and interfaces calculated for different adherends.

A Novel Source/Drain-to-Gate Non-overlap MOSFET to Reduce Gate Leakage Current in Nano Regime

In this paper, gate leakage current has been mitigated by the use of novel nanoscale MOSFET with Source/Drain-to-Gate Non-overlapped and high-k spacer structure for the first time. A compact analytical model has been developed to study the gate leakage behaviour of proposed MOSFET structure. The result obtained has found good agreement with the Sentaurus Simulation. Fringing gate electric field through the dielectric spacer induces inversion layer in the non-overlap region to act as extended S/D region. It is found that optimal Source/Drain-to-Gate Non-overlapped and high-k spacer structure has reduced the gate leakage current to great extent as compared to those of an overlapped structure. Further, the proposed structure had improved off current, subthreshold slope and DIBL characteristic. It is concluded that this structure solves the problem of high leakage current without introducing the extra series resistance.

Silicon Nanowire for Thermoelectric Applications: Effects of Contact Resistance

Silicon nanowire (SiNW) based thermoelectric device (TED) has potential applications in areas such as chip level cooling/ energy harvesting. It is a great challenge however, to assemble an efficient device with these SiNW. The presence of parasitic in the form of interfacial electrical resistance will have a significant impact on the performance of the TED. In this work, we explore the effect of the electrical contact resistance on the performance of a TED. Numerical simulations are performed on SiNW to investigate such effects on its cooling performance. Intrinsically, SiNW individually without the unwanted parasitic effect has excellent cooling power density. However, the cooling effect is undermined with the contribution of the electrical contact resistance.

Effect of TCSR on Measured Impedance by Distance Protection in Presence Single Phase to Earth Fault

This paper presents the impact study of apparent reactance injected by series Flexible AC Transmission System (FACTS) i.e. Thyristor Controlled Series Reactor (TCSR) on the measured impedance of a 400 kV single electrical transmission line in the presence of phase to earth fault with fault resistance. The study deals with an electrical transmission line of Eastern Algerian transmission networks at Group Sonelgaz (Algerian Company of Electrical and Gas) compensated by TCSR connected at midpoint of the line. This compensator used to inject active and reactive powers is controlled by three TCSR-s. The simulations results investigate the impacts of the TCSR on the parameters of short circuit calculation and parameters of measured impedance by distance relay in the presence of earth fault for three cases study.

Improvement of Durability of Wood by Maleic Anhydride

Wood as a natural renewable material is vulnerable to degradation by microorganisms and susceptible to change in dimension by water. In order to effectively improve the durability of wood, an active reagent, maleic anhydride (Man) was selected for wood modification. Man was first dissolved into a solvent, and then penetrated into wood porous structure under a vacuum/pressure condition. After a final catalyst-thermal treatment, wood modification was finished. The test results indicate that acetone is a good solvent for transporting Man into wood matrix. SEM observation proved that wood samples treated by Man kept a good cellular structure, indicating a well penetration of Man into wood cell walls. FTIR analysis suggested that Man reacted with hydroxyl groups on wood cell walls by its ring-ether group, resulting in reduction of amount of hydroxyl groups and resultant good dimensional stability as well as fine decay resistance. Consequently, Man modifying wood to improve its durability is an effective method.

An Investigation of the Effect of the Different Mix Constituents on Concrete Electric Resistivity

Steel corrosion in concrete is considered as a main engineering problems for many countries and lots of expenses has been paid for their repair and maintenance annually. This problem may occur in all engineering structures whether in coastal and offshore or other areas. Hence, concrete structures should be able to withstand corrosion factors existing in water or soil. Reinforcing steel corrosion enhancement can be measured by use of concrete electrical resistance; and maintaining high electric resistivity in concrete is necessary for steel corrosion prevention. Lots of studies devoted to different aspects of the subjects worldwide. In this paper, an evaluation of the effects of W/C ratio, cementitious materials, and percent increase in silica fume were investigated on electric resistivity of high strength concrete. To do that, sixteen mix design with one aggregate grading was planned. Five of them had varying amount of W/C ratio and other eleven mixes was prepared with constant W/C ratio but different amount of cementitious materials. Silica fume and super plasticizer were used with different proportions in all specimens. Specimens were tested after moist curing for 28 days. A total of 80 cube specimens (50 mm) were tested for concrete electrical resistance. Results show that concrete electric resistivity can be increased with increasing amount of cementitious materials and silica fume.

Novel Structural Insights of Glutamate Racemase from Mycobacterium tuberculosis through Modeling and Docking Studies

An alarming emergence of multidrug-resistant strains of the tuberculosis pathogen Mycobacterium tuberculosis and continuing high worldwide incidence of tuberculosis has invigorated the search for novel drug targets. The enzyme glutamate racemase (MurI) in bacteria catalyzes the stereoconversion of L-glutamate to D-glutamate which is a component of the peptidoglycan cell wall of the bacterium. The inhibitors targeted against MurI from several bacterial species have been patented and are advocated as promising antibacterial agents. However there are none available against MurI from Mycobacterium tuberculosis, due to the lack of its threedimensional structure. This work accomplished two major objectives. First, the tertiary structure of MtMurI was deduced computationally through homology modeling using the templates from bacterial homologues. It is speculated that like in other Gram-positive bacteria, MtMurI exists as a dimer and many of the protein interactions at the dimer interface are also conserved. Second, potent candidate inhibitors against MtMurI were identified through docking against already known inhibitors in other organisms.

Performance of Membrane Bioreactor (MBR) in High Phosphate Wastewater

This study presents the performance of membrane bioreactor in treating high phosphate wastewater. The laboratory scale MBR was operated at permeate flux of 25 L/m2.h with a hollow fiber membrane (polypropylene, approx. pore size 0.01 - 0.2 μm) at hydraulic retention time (HRT) of 12 hrs. Scanning electron microscopy (SEM) and energy diffusive X-ray (EDX) analyzer were used to characterize the membrane foulants. Results showed that the removal efficiencies of COD, TSS, NH3-N and PO4 3- were 93, 98, 80 and 30% respectively. On average 91% of influent soluble microbial products (SMP) were eliminated, with the eliminations of polysaccharides mostly above 80%. The main fouling resistance was cake resistance. It should be noted that SMP were found in major portions of mixed liquor that played a relatively significant role in membrane fouling. SEM and EDX analyses indicated that the foulants covering the membrane surfaces comprises not only organic substances but also inorganic elements including Mg, Ca, Al, K and P.