Abstract: A suitable model membrane to study the pharmacological effect of pharmaceutical products is human stratum corneum because this layer of human skin is the outermost layer and it is an important barrier to be passed through. Other model membranes which were also used are for example skins from pig, mouse, reptile or fish. We are interested in fish skins in this project. The advantages of the fish skins are, that they can be obtained from the supermarket or fish shop. However, the fish skins should be freshly prepared and used directly without storage. In order to understand the effect of different model drugs e.g. lidocaine HCl, resveratrol, paracetamol, ibuprofen, acetyl salicylic acid on the properties of the model membrane from various types of fishes e.g. trout, salmon, cod, plaice permeation tests were performed and differential scanning calorimetry was applied.
Abstract: In this article, the antibiogram and heavy metal
resistance profile of the bacteria isolated from total 34 studied
animals (Pelophylax ridibundus = 12; Mauremys rivulata = 14;
Natrix natrix = 8) captured around the Biga Stream, are described.
There was no database information on antibiogram and heavy metal
resistance profile of bacteria from these area’s amphibians and
reptiles.
A total of 200 bacteria were successfully isolated from cloaca and
oral samples of the aquatic amphibians and reptiles as well as from
the water sample. According to Jaccard’s similarity index, the degree
of similarity in the bacterial flora was quite high among the
amphibian and reptile species under examination, whereas it was
different from the bacterial diversity in the water sample. The most
frequent isolates were A. hydrophila (31.5%), B. pseudomallei
(8.5%), and C. freundii (7%). The total numbers of bacteria obtained
were as follows: 45 in P. ridibundus, 45 in N. natrix 30 in M.
rivulata, and 80 in the water sample. The result showed that
cefmetazole was the most effective antibiotic to control the bacteria
isolated in this study and that approximately 93.33% of the bacterial
isolates were sensitive to this antibiotic. The multiple antibiotic
resistances (MAR) index indicated that P. ridibundus (0.95) > N.
natrix (0.89) > M. rivulata (0.39). Furthermore, all the tested heavy
metals (Pb+2, Cu+2, Cr+3, and Mn+2) inhibit the growth of the bacterial
isolates at different rates. Therefore, it indicated that the water source
of the animals was contaminated with both antibiotic residues and
heavy metals.