Abstract: In this study, the in vitro anticholinesterase activity of
the volatile oils of both O. basilicum and O. africanum was
investigated and both samples showed significant activity. The major
constituents of the two oils were isolated using several column
chromatographies. Linalool, 1,8-cineol and eugenol were isolated
from the volatile oil of O. basilicum and camphor was isolated from
the volatile oil of O. africanum. The anticholinesterase activities of
the isolated compounds were also evaluated where 1,8-cineol showed
the highest inhibitory activity followed by camphor. To confirm these
activities, learning and memory enhancing effects were tested in
mice. Memory impairment was induced by scopolamine, a
cholinergic muscarinic receptor antagonist. Anti-amnesic effects of
both volatile oils and their terpenoids were investigated by the
passive avoidance task in mice. We also examined their effects on
brain acetylcholinesterase activity. Results showed that scopolamineinduced
cognitive dysfunction was significantly attenuated by
administration of the volatile oils and their terpenoids, eugenol and
camphor, in the passive avoidance task and inhibited brain
acetylcholinesterase activity. These results suggest that O. basilicum
and O. africanum volatile oils can be good candidates for further
studies on Alzheimer’s disease via their acetylcholinesterase
inhibitory actions.
Abstract: Intercropping is one of the sustainable agricultural
factors. The SPAD meter can be used to predict nitrogen index
reliably, it may also be a useful tool for assessing the relative impact
of weeds on crops. In order to study the effect of weeds on SPAD in
corn (Zea mays L.), sweet basil (Ocimum basilicum L.) and borage
(Borago officinalis L.) in intercropping system, a factorial experiment
was conducted in three replications in 2011. Experimental factors
were included intercropping of corn with sweet basil and borage in
different ratios (100:0, 75:25, 50:50, 25:75 and 0:100 corn: borage or
sweet basil) and weed infestation (weed control and weed
interference). The results showed that intercropping of corn with
sweet basil and borage increased the SPAD value of corn compare to
monoculture in weed interference condition. Sweet basil SPAD value
in weed control treatments (43.66) was more than weed interference
treatments (40.17). Corn could increase the borage SPAD value
compare to monoculture in weed interference treatments.