Abstract: The overall objective of this research is a strain
improvement technology for efficient pectinase production. A novel
cells cultivation technology by immobilization of fungal cells has
been studied in long time continuous fermentations. Immobilization
was achieved by using of new material for absorption of stores of
immobilized cultures which was for the first time used for
immobilization of microorganisms. Effects of various conditions of
nitrogen and carbon nutrition on the biosynthesis of pectolytic
enzymes in Aspergillus awamori 1-8 strain were studied. Proposed
cultivation technology along with optimization of media components
for pectinase overproduction led to increased pectinase productivity
in Aspergillus awamori 1-8 from 7 to 8 times. Proposed technology
can be applied successfully for production of major industrial
enzymes such as α-amylase, protease, collagenase etc.
Abstract: Expression data analysis is based mostly on the
statistical approaches that are indispensable for the study of
biological systems. Large amounts of multidimensional data resulting
from the high-throughput technologies are not completely served by
biostatistical techniques and are usually complemented with visual,
knowledge discovery and other computational tools. In many cases,
in biological systems we only speculate on the processes that are
causing the changes, and it is the visual explorative analysis of data
during which a hypothesis is formed. We would like to show the
usability of multidimensional visualization tools and promote their
use in life sciences. We survey and show some of the
multidimensional visualization tools in the process of data
exploration, such as parallel coordinates and radviz and we extend
them by combining them with the self-organizing map algorithm. We
use a time course data set of transitional cell carcinoma of the bladder
in our examples. Analysis of data with these tools has the potential to
uncover additional relationships and non-trivial structures.
Abstract: This study explored the relationship between
occupational stress and the perceived effectiveness of men and
women managers in Ghanaian organizations. The exploration is
underlined by attempt to understand the degree to which male and
female managers in Ghanaian organizations experience occupational
stress at the workplace. The purpose is to examine the sources and
extents of occupational stress experienced by male and female
managers in Ghana. Data was collected using questionnaires and
analyzed using both descriptive statistics and correlation analysis.
The results showed that female managers in Ghana are more likely to
report of more stress experiences in the workplace than their male
counterparts. The female managers are more likely to perceive role
conflict and alienation as job stressors while the male managers
perceived blocked career as a major source of workplace stress. It is
concluded that despite the female managers experiencing enormous
level of occupational stress, there was no significant differences
between their managerial effectiveness and that of the male.
Abstract: The steady-state temperature for one-dimensional transpiration cooling system has been conducted experimentally and numerically to investigate the heat transfer characteristics of combined convection and radiation. The Nickel –Chrome (Ni-Cr) open-cellular porous material having porosity of 0.93 and pores per inch (PPI) of 21.5 was examined. The upper surface of porous plate was heated by the heat flux of incoming radiation varying from 7.7 - 16.6 kW/m2 whereas air injection velocity fed into the lower surface was varied from 0.36 - 1.27 m/s, and was then rearranged as Reynolds number (Re). For the report of the results in the present study, two efficiencies including of temperature and conversion efficiency were presented. Temperature efficiency indicating how close the mean temperature of a porous heat plate to that of inlet air, and increased rapidly with the air injection velocity (Re). It was then saturated and had a constant value at Re higher than 10. The conversion efficiency, which was regarded as the ability of porous material in transferring energy by convection after absorbed from heat radiation, decreased with increasing of the heat flux and air injection velocity. In addition, it was then asymptotic to a constant value at the Re higher than 10. The numerical predictions also agreed with experimental data very well.
Abstract: Alpfa-fetoprotein and its fragments may be an important vehicle for targeted delivery of radionuclides to the tumor. We investigated the effect of conditions on the labeling of biologically active synthetic peptide based on the (F-afp) with technetium-99m. The influence of the nature of the buffer solution, pH, concentration of reductant, concentration of the peptide and the reaction temperature on the yield of labeling was examined. As a result, the following optimal conditions for labeling of (F-afp) are found: pH 8.5 (phosphate and bicarbonate buffers) and pH from 1.7 to 7.0 (citrate buffer). The reaction proceeds with sufficient yield at room temperature for 30 min at the concentration of SnCl2 and (Fafp) (F-afp) is to be less than 10 mkg/ml and 25 mkg/ml, respectively. Investigations of the test drug accumulation in the tumor cells of human breast cancer were carried out. Results can be assumed that the in vivo study of the (F-afp) in experimental tumor lesions will show concentrations sufficient for imaging these lesions by SPECT.
Abstract: We report a computational study of the spreading
dynamics of a viral infection in a complex (scale-free) network. The
final epidemic size distribution (FESD) was found to be unimodal or
bimodal depending on the value of the basic reproductive
number R0 . The FESDs occurred on time-scales long enough for
intermediate-time epidemic size distributions (IESDs) to be important
for control measures. The usefulness of R0 for deciding on the
timeliness and intensity of control measures was found to be limited
by the multimodal nature of the IESDs and by its inability to inform
on the speed at which the infection spreads through the population. A
reduction of the transmission probability at the hubs of the scale-free
network decreased the occurrence of the larger-sized epidemic events
of the multimodal distributions. For effective epidemic control, an
early reduction in transmission at the index cell and its neighbors was
essential.
Abstract: In this paper a new fast simplification method is presented. Such method realizes Karnough map with large number of variables. In order to accelerate the operation of the proposed method, a new approach for fast detection of group of ones is presented. Such approach implemented in the frequency domain. The search operation relies on performing cross correlation in the frequency domain rather than time one. It is proved mathematically and practically that the number of computation steps required for the presented method is less than that needed by conventional cross correlation. Simulation results using MATLAB confirm the theoretical computations. Furthermore, a powerful solution for realization of complex functions is given. The simplified functions are implemented by using a new desigen for neural networks. Neural networks are used because they are fault tolerance and as a result they can recognize signals even with noise or distortion. This is very useful for logic functions used in data and computer communications. Moreover, the implemented functions are realized with minimum amount of components. This is done by using modular neural nets (MNNs) that divide the input space into several homogenous regions. Such approach is applied to implement XOR function, 16 logic functions on one bit level, and 2-bit digital multiplier. Compared to previous non- modular designs, a clear reduction in the order of computations and hardware requirements is achieved.
Abstract: Shadoo protein (Sho) was described in 2003 as the newest member of Prion protein superfamily [1]. Sho has similar structural motifs like prion protein (PrP) that is known for its central role in transmissible spongiform enchephalopathies. Although a great number of functions have been proposed, the exact physiological function of PrP is not known yet. Investigation of the function and localization of Sho may help us to understand the function of the Prion protein superfamily. Analyzing the subcellular localization of YFP-tagged forms of Sho, we detected the protein in the plasma membrane and in the nucleus of various cell lines. To reveal the localization of the endogenous protein we generated antibodies against Shadoo as well as employed commercially available anti-Shadoo antibodies: i) EG62 anti-mouse Shadoo antibody generated by Eurogentec Ltd.; ii) S-12 anti-human Shadoo antibody by Santa Cruz Biotechnology Inc.; iii) R-12 anti-mouse Shadoo antibody by Santa Cruz Biotechnology Inc.; iv) SPRN antibody against human Shadoo by Abgent Inc. We carried out immunocytochemistry on non-transfected HeLa, Zpl 2-1, Zw 3-5, GT1-1, GT1-7 and SHSY5Y cells as well as on YFP-Sho, Sho-YFP, and YFP-GPI transfected HeLa cells. Their specificity (in antibody-peptide competition assay) and co-localization (with the YFP signal) were assessed.
Abstract: Fine-grained data replication over the Internet allows duplication of frequently accessed data objects, as opposed to entire sites, to certain locations so as to improve the performance of largescale content distribution systems. In a distributed system, agents representing their sites try to maximize their own benefit since they are driven by different goals such as to minimize their communication costs, latency, etc. In this paper, we will use game theoretical techniques and in particular auctions to identify a bidding mechanism that encapsulates the selfishness of the agents, while having a controlling hand over them. In essence, the proposed game theory based mechanism is the study of what happens when independent agents act selfishly and how to control them to maximize the overall performance. A bidding mechanism asks how one can design systems so that agents- selfish behavior results in the desired system-wide goals. Experimental results reveal that this mechanism provides excellent solution quality, while maintaining fast execution time. The comparisons are recorded against some well known techniques such as greedy, branch and bound, game theoretical auctions and genetic algorithms.
Abstract: Hemorrhage Disease of Grass Carp (HDGC) is a kind
of commonly occurring illnesses in summer, and the extremely high
death rate result in colossal losses to aquaculture. As the complex
connections among each factor which influences aquiculture diseases,
there-s no quit reasonable mathematical model to solve the problem at
present.A BP neural network which with excellent nonlinear mapping
coherence was adopted to establish mathematical model;
Environmental factor, which can easily detected, such as breeding
density, water temperature, pH and light intensity was set as the main
analyzing object. 25 groups of experimental data were used for
training and test, and the accuracy of using the model to predict the
trend of HDGC was above 80%. It is demonstrated that BP neural
network for predicating diseases in HDGC has a particularly
objectivity and practicality, thus it can be spread to other aquiculture
disease.
Abstract: This paper proposes an effective adaptation learning
algorithm based on artificial neural networks for speed control of an
induction motor assumed to operate in a high-performance drives
environment. The structure scheme consists of a neural network
controller and an algorithm for changing the NN weights in order that
the motor speed can accurately track of the reference command. This
paper also makes uses a very realistic and practical scheme to
estimate and adaptively learn the noise content in the speed load
torque characteristic of the motor. The availability of the proposed
controller is verified by through a laboratory implementation and
under computation simulations with Matlab-software. The process is
also tested for the tracking property using different types of reference
signals. The performance and robustness of the proposed control
scheme have evaluated under a variety of operating conditions of the
induction motor drives. The obtained results demonstrate the
effectiveness of the proposed control scheme system performances,
both in steady state error in speed and dynamic conditions, was found
to be excellent and those is not overshoot.
Abstract: The study investigated the hydrophilic to hydrophobic
transition of modified polyacrylamide hydrogel with the inclusion of
N-isopropylacrylamide (NIAM). The modification was done by
mimicking micellar polymerization, which resulted in better
arrangement of NIAM chains in the polyacrylamide network. The
degree of NIAM arrangement is described by NH number. The
hydrophilic to hydrophobic transition was measured through the
partition coefficient, K, of Orange II and Methylene Blue in hydrogel
and in water. These dyes were chosen as a model for solutes with
different degree of hydrophobicity. The study showed that the
hydrogel with higher NH values resulted in better solubility of both
dyes. Moreover, in temperature above the lower critical solution
temperature (LCST) of Poly(N-isopropylacrylamide) (PNIAM)also
caused the collapse of NIPAM chains which results in a more
hydrophobic environment that increases the solubility of Methylene
Blue and decreases the solubility of Orange II in the hydrogels with
NIPAM present.
Abstract: In this paper, an algorithm for detecting and attenuating
puff noises frequently generated under the mobile environment is
proposed. As a baseline system, puff detection system is designed
based on Gaussian Mixture Model (GMM), and 39th Mel Frequency
Cepstral Coefficient (MFCC) is extracted as feature parameters. To
improve the detection performance, effective acoustic features for puff
detection are proposed. In addition, detected puff intervals are
attenuated by high-pass filtering. The speech recognition rate was
measured for evaluation and confusion matrix and ROC curve are used
to confirm the validity of the proposed system.
Abstract: Sugarcane Shoots is an abundantly available
residual resources consisting of lignocelluloses which take it into
the benefit. The present study was focused on utilizing of
sugarcane shoot for reducing sugar production as a substrate in
ethanol production. Physical and chemical pretreatments of
sugarcane shoot were investigated. Results showed that the size of
sugarcane shoot influenced the cellulose content. The maximum
cellulose yield (60 %) can be obtained from alkaline pretreated
sugarcane shoot with 1.0 M NaOH at 30 oC for 90 min. The
cellulose yield reached up to 93.9% (w/w). Enzymatically
hydrolyzed of cellulosic residual in 0.04 citrate buffer (pH 5) with
celluclast 1.5L (0.7 FPU/ml) resulted in the highest amount of
reducing sugar at a rate of 32.1 g/l after 4 h incubation at 50°C,
and 100 oC for 5 min . Cellulose conversion was 55.5%.
Abstract: Despite the fact that Arabic language is currently one
of the most common languages worldwide, there has been only a
little research on Arabic speech recognition relative to other
languages such as English and Japanese. Generally, digital speech
processing and voice recognition algorithms are of special
importance for designing efficient, accurate, as well as fast automatic
speech recognition systems. However, the speech recognition process
carried out in this paper is divided into three stages as follows: firstly,
the signal is preprocessed to reduce noise effects. After that, the
signal is digitized and hearingized. Consequently, the voice activity
regions are segmented using voice activity detection (VAD)
algorithm. Secondly, features are extracted from the speech signal
using Mel-frequency cepstral coefficients (MFCC) algorithm.
Moreover, delta and acceleration (delta-delta) coefficients have been
added for the reason of improving the recognition accuracy. Finally,
each test word-s features are compared to the training database using
dynamic time warping (DTW) algorithm. Utilizing the best set up
made for all affected parameters to the aforementioned techniques,
the proposed system achieved a recognition rate of about 98.5%
which outperformed other HMM and ANN-based approaches
available in the literature.
Abstract: By the application of an improved back-propagation
neural network (BPNN), a model of current densities for a solid oxide
fuel cell (SOFC) with 10 layers is established in this study. To build
the learning data of BPNN, Taguchi orthogonal array is applied to
arrange the conditions of operating parameters, which totally 7 factors
act as the inputs of BPNN. Also, the average current densities
achieved by numerical method acts as the outputs of BPNN.
Comparing with the direct solution, the learning errors for all learning
data are smaller than 0.117%, and the predicting errors for 27
forecasting cases are less than 0.231%. The results show that the
presented model effectively builds a mathematical algorithm to predict
performance of a SOFC stack immediately in real time.
Also, the calculating algorithms are applied to proceed with the
optimization of the average current density for a SOFC stack. The
operating performance window of a SOFC stack is found to be
between 41137.11 and 53907.89. Furthermore, an inverse predicting
model of operating parameters of a SOFC stack is developed here by
the calculating algorithms of the improved BPNN, which is proved to
effectively predict operating parameters to achieve a desired
performance output of a SOFC stack.
Abstract: The effects of coatings based on sodium alginate (S.A) and carboxyl methyl cellulose (CMC) on the color and moisture characteristics of potato round slices were investigated. It is the first time that this combination of polysaccharides is used as edible coating which alone had the best performance as inhibitor of potato color discoloration during the storage of 15 days at 4oC. When ascorbic acid (AA) and green tea (GT) were added in the above edible coating its effects on potato round slices changed. The mixtures of sodium alginate and carboxyl methyl cellulose with ascorbic acid or with green tea behave as a potential moisture barrier, resulting to the extent of potato samples self–life. These data suggests that both GT and AA are potential inhibitors of dehydration in potatoes and not only natural antioxidants.
Abstract: A convenient and physically sound mathematical model of the external or I - V characteristic of solar cells generators is presented in this paper. This model is compared with the traditional model of p-n junction. The direct analytical calculation of load regime leads to a quadratic equation, which is importantly to simplify the calculations in the real time.
Abstract: fibers of pure cellulose can be made from some bacteria such as acetobacter xylinum. Bacterial cellulose fibers are very pure, tens of nm across and about 0.5 micron long. The fibers are very stiff and, although nobody seems to have measured the strength of individual fibers. Their stiffness up to 70 GPa. Fundamental strengths should be at least greater than those of the best commercial polymers, but best bulk strength seems to about the same as that of steel. They can potentially be produced in industrial quantities at greatly lowered cost and water content, and with triple the yield, by a new process. This article presents a critical review of the available information on the bacterial cellulose as a biological nonwoven fabric with special emphasis on its fermentative production and applications. Characteristics of bacterial cellulose biofabric with respect to its structure and physicochemical properties are discussed. Current and potential applications of bacterial cellulose in textile, nonwoven cloth, paper, films synthetic fiber coating, food, pharmaceutical and other industries are also presented.
Abstract: The objective of this paper is to support the application of Open Innovation practices in firms and organizations by the assessment and management of Intellectual Capital. Intellectual Capital constituents are analyzed in order to verify their capability of acting as key drivers of Open Innovation processes and, therefore, of creating value. A methodology is defined to settle a procedure which helps to select the most relevant Intellectual Capital value drivers and to provide Communities of Innovation with strategic and managerial guidelines in sustaining Open Innovation paradigm. An application of the methodology is developed within a specifically addressed project and its results are hereafter examined.