Abstract: β-Glucosidase is an important enzyme for production
of ethanol from lignocellulose. With hydrolytic activity on
cellooligosaccharides, especially cellobiose, β-glucosidase removes
product inhibitory effect on cellulases and forms fermentable sugars.
In this study, β-glucosidase encoding gene (BGL1) from traditional
starter yeast Saccharomycosis fibuligera BMQ908 was cloned and
expressed in Pichia pastoris. BGL1 of S. fibuligera BMQ 908 shared
98% nucleotide homology with the closest GenBank sequence
(M22475) but identity in amino-acid sequences of catalytic domains.
Recombinant plasmid pPICZαA/BGL1 containing the sequence
encoding BGL1 mature protein and α-factor secretion signal was
constructed and transformed into methylotrophic yeast P. pastoris by
electroporation. The recombinant strain produced single extracellular
protein with molecular weight of 120 kDa and cellobiase activity of
60 IU/ml. The optimum pH of the recombinant β-glucosidase was 5.0
and the optimum temperature was 50°C.
Abstract: This paper presents a complete procedure for tool path
planning and blade machining in 5-axis manufacturing. The actual
cutting contact and cutter locations can be determined by lead and tilt
angles. The tool path generation is implemented by piecewise curved
approximation and chordal deviation detection. An application about
drive surface method promotes flexibility of tool control and stability
of machine motion. A real manufacturing process is proposed to
separate the operation into three regions with five stages and to modify
the local tool orientation with an interactive algorithm.
Abstract: A new conceptual architecture for low-level neural
pattern recognition is presented. The key ideas are that the brain
implements support vector machines and that support vectors are
represented as memory patterns in competitive queuing memories. A
binary classifier is built from two competitive queuing memories
holding positive and negative valence training examples respectively.
The support vector machine classification function is calculated in
synchronized evaluation cycles. The kernel is computed by bisymmetric
feed-forward networks feed by sensory input and by
competitive queuing memories traversing the complete sequence of
support vectors. Temporary summation generates the output
classification. It is speculated that perception apparatus in the brain
reuses structures that have evolved for enabling fluent execution of
prepared action sequences so that pattern recognition is built on
internalized motor programmes.
Abstract: The purpose of this article is to identify the practical strategies of R&D (research and development) entities for developing converging technology in organizational context. Based on the multi-assignation technological domains of patents derived from entire government-supported R&D projects for 13 years, we find that technology convergence is likely to occur when a university solely develops technology or when university develops technology as one of the collaborators. These results reflect the important role of universities in developing converging technology
Abstract: The transformation of vocal characteristics aims at
modifying voice such that the intelligibility of aphonic voice is
increased or the voice characteristics of a speaker (source speaker) to
be perceived as if another speaker (target speaker) had uttered it. In
this paper, the current state-of-the-art voice characteristics
transformation methodology is reviewed. Special emphasis is placed
on voice transformation methodology and issues for improving the
transformed speech quality in intelligibility and naturalness are
discussed. In particular, it is suggested to use the modulation theory
of speech as a base for research on high quality voice transformation.
This approach allows one to separate linguistic, expressive, organic
and perspective information of speech, based on an analysis of how
they are fused when speech is produced. Therefore, this theory
provides the fundamentals not only for manipulating non-linguistic,
extra-/paralinguistic and intra-linguistic variables for voice
transformation, but also for paving the way for easily transposing the
existing voice transformation methods to emotion-related voice
quality transformation and speaking style transformation. From the
perspectives of human speech production and perception, the popular
voice transformation techniques are described and classified them
based on the underlying principles either from the speech production
or perception mechanisms or from both. In addition, the advantages
and limitations of voice transformation techniques and the
experimental manipulation of vocal cues are discussed through
examples from past and present research. Finally, a conclusion and
road map are pointed out for more natural voice transformation
algorithms in the future.
Abstract: Delivering course material via a virtual environment
is beneficial to today-s students because it offers the interactivity,
real-time interaction and social presence that students of all ages
have come to accept in our gaming rich community. It is essential
that the Net Generation also known as Generation Why, have
exposure to learning communities that encompass interactivity to
form social and educational connections. As student and professor
become interconnected through collaboration and interaction in a
virtual learning space, relationships develop and students begin to
take on an individual identity. With this in mind the research project
was developed to investigate the use of virtual environments on
student satisfaction and the effectiveness of course delivery.
Furthermore, the project was designed to integrate both interactive
(real-time) classes conducted in the Virtual Reality (VR)
environment while also creating archived VR sessions for student use
in retaining and reviewing course content.
Abstract: Since the European renewable energy directives set the
target for 22.1% of electricity generation to be supplied by 2010
[1], there has been increased interest in using green technologies
also within the urban enviroment. The most commonly considered
installations are solar thermal and solar photovoltaics. Nevertheless,
as observed by Bahaj et al. [2], small scale turbines can reduce the
built enviroment related CO2 emissions. Thus, in the last few years,
an increasing number of manufacturers have developed small wind
turbines specifically designed for the built enviroment. The present
work focuses on the integration into architectural systems of such
installations and presents a survey of successful case studies.
Abstract: This paper explores gender related barriers to interagency collaboration in statutory children safeguard partnerships against a theoretical framework that considers individuals, professions and organisations interacting as part of a complex adaptive system. We argue that gender-framed obstacles to effective communication between culturally discrepant agencies can ultimately impact on the effectiveness of policy delivery,. We focused our research on three partnership structures in Sefton Metropolitan Borough in order to observe how interactions occur, whether the agencies involved perceive their occupational environment as being gender affected and whether they believe this can hinder effective collaboration with other biased organisations. Our principal empirical findings indicate that there is a general awareness amongst professionals of the role that gender plays in each of the agencies reviewed, that gender may well constitute a barrier to effective communication, but there is a sense in which there is little scope for change in the short term. We aim to signal here, however, the need to change against the risk of service failure.
Abstract: The Prediction of aerodynamic characteristics and
shape optimization of airfoil under the ground effect have been carried
out by integration of computational fluid dynamics and the multiobjective
Pareto-based genetic algorithm. The main flow
characteristics around an airfoil of WIG craft are lift force, lift-to-drag
ratio and static height stability (H.S). However, they show a strong
trade-off phenomenon so that it is not easy to satisfy the design
requirements simultaneously. This difficulty can be resolved by the
optimal design. The above mentioned three characteristics are chosen
as the objective functions and NACA0015 airfoil is considered as a
baseline model in the present study. The profile of airfoil is
constructed by Bezier curves with fourteen control points and these
control points are adopted as the design variables. For multi-objective
optimization problems, the optimal solutions are not unique but a set
of non-dominated optima and they are called Pareto frontiers or Pareto
sets. As the results of optimization, forty numbers of non- dominated
Pareto optima can be obtained at thirty evolutions.
Abstract: The article describes a case study on one of Czech
Republic-s manufacturing middle size enterprises (ME), where due to
the European financial crisis, production lines had to be redesigned
and optimized in order to minimize the total costs of the production
of goods. It is considered an optimization problem of minimizing the
total cost of the work load, according to the costs of the possible
locations of the workplaces, with an application of the Greedy
algorithm and a partial analogy to a Set Packing Problem. The
displacement of working tables in a company should be as a one-toone
monotone increasing function in order for the total costs of
production of the goods to be at minimum. We use a heuristic
approach with greedy algorithm for solving this linear optimization
problem, regardless the possible greediness which may appear and
we apply it in a Czech ME.
Abstract: This study deals with Computational Fluid Dynamics
(CFD) studies of the interactions between the air flow and louvered
fins which equipped the automotive heat exchangers. 3D numerical
simulation results are obtained by using the ANSYS Fluent 13.0 code
and compared to experimental data. The paper studies the effect of
louver angle and louver pitch geometrical parameters, on overall
thermal hydraulic performances of louvered fins.
The comparison between CFD simulations and experimental data
show that established 3-D CFD model gives a good agreement. The
validation agrees, with about 7% of deviation respectively of friction
and Colburn factors to experimental results. As first, it is found that
the louver angle has a strong influence on the heat transfer rate. Then,
louver angle and louver pitch variation of the louvers and their effects
on thermal hydraulic performances are studied. In addition to this
study, it is shown that the second half of the fin takes has a
significant contribution on pressure drop increase without any
increase in heat transfer.
Abstract: Cameron Highlands is known for upland tourism area
with vast natural wealth, mountainous landscape endowed with rich
diverse species as well as people traditions and cultures. With these
various resources, CH possesses an interesting visual and panorama
that can be offered to the tourist. However this benefit may not be
utilized without obtaining the understanding of existing landscape
structure and visual. Given a limited data, this paper attempts to
classify landscape visual of Cameron Highlands using land use and
contour data. Visual points of view were determined from the given
tourist attraction points in the CH Local Plan 2003-2015. The result
shows landscape visual and structure categories offered in the study
area. The result can be used for further analysis to determine the best
alternative tourist trails for tourism planning and decision making
using readily available data.
Abstract: The major purpose of this study is to use network and multimedia technologies to build a game-based learning system for junior high school students to apply in learning “World Geography" through the “role-playing" game approaches. This study first investigated the motivation and habits of junior high school students to use the Internet and online games, and then designed a game-based learning system according to situated and game-based learning theories. A teaching experiment was conducted to analyze the learning effectiveness of students on the game-based learning system and the major factors affecting their learning. A questionnaire survey was used to understand the students- attitudes towards game-based learning. The results showed that the game-based learning system can enhance students- learning, but the gender of students and their habits in using the Internet have no significant impact on learning. Game experience has a significant impact on students- learning, and the higher the experience value the better the effectiveness of their learning. The results of questionnaire survey also revealed that the system can increase students- motivation and interest in learning "World Geography".
Abstract: Prime Factorization based on Quantum approach in
two phases has been performed. The first phase has been achieved at
Quantum computer and the second phase has been achieved at the
classic computer (Post Processing). At the second phase the goal is to
estimate the period r of equation xrN ≡ 1 and to find the prime factors
of the composite integer N in classic computer. In this paper we
present a method based on Randomized Approach for estimation the
period r with a satisfactory probability and the composite integer N
will be factorized therefore with the Randomized Approach even the
gesture of the period is not exactly the real period at least we can find
one of the prime factors of composite N. Finally we present some
important points for designing an Emulator for Quantum Computer
Simulation.
Abstract: In this paper, we present a new and effective image indexing technique that extracts features directly from DCT domain. Our proposed approach is an object-based image indexing. For each block of size 8*8 in DCT domain a feature vector is extracted. Then, feature vectors of all blocks of image using a k-means algorithm is clustered into groups. Each cluster represents a special object of the image. Then we select some clusters that have largest members after clustering. The centroids of the selected clusters are taken as image feature vectors and indexed into the database. Also, we propose an approach for using of proposed image indexing method in automatic image classification. Experimental results on a database of 800 images from 8 semantic groups in automatic image classification are reported.
Abstract: Sharing motivations of viral advertisements by
consumers and the impacts of these advertisements on the
perceptions for brand will be questioned in this study. Three
fundamental questions are answered in the study. These are
advertisement watching and sharing motivations of individuals,
criteria of liking viral advertisement and the impact of individual
attitudes for viral advertisement on brand perception respectively.
This study will be carried out via a viral advertisement which was
practiced in Turkey. The data will be collected by survey method and
the sample of the study consists of individuals who experienced the
practice of sample advertisement. Data will be collected by online
survey method and will be analyzed by using SPSS statistical
package program.
Recently traditional advertisement mind have been changing. New
advertising approaches which have significant impacts on consumers
have been argued. Viral advertising is a modernist advertisement
mind which offers significant advantages to brands apart from
traditional advertising channels such as television, radio and
magazines. Viral advertising also known as Electronic Word-of-
Mouth (eWOM) consists of free spread of convincing messages sent
by brands among interpersonal communication. When compared to
the traditional advertising, a more provocative thematic approach is
argued.
The foundation of this approach is to create advertisements that
are worth sharing with others by consumers. When that fact is taken
into consideration, in a manner of speaking it can also be stated that
viral advertising is media engineering.
The content worth sharing makes people being a volunteer
spokesman of a brand and strengthens the emotional bonds among
brand and consumer. Especially for some sectors in countries which
are having traditional advertising channel limitations, viral
advertising creates vital advantages.
Abstract: A 3D industrial computed tomography (CT)
manufactured based on a first generation CT systems, single-source
– single-detector, was evaluated. Operation accuracy assessment of
the manufactured system was achieved using simulation in
comparison with experimental tests. 137Cs and 60Co were used as a gamma source. Simulations were achieved using MCNP4C code.
Experimental tests of 137Cs were in good agreement with the simulations
Abstract: This paper addresses the problem of building a unified
structure to describe a peer-to-peer system. Our approach uses the
well-known notations in the P2P area, and provides a global
architecture that puts a separation between the platform specific
characteristics and the logical ones. In order to enable the navigation
of the peer across platforms, a roaming layer is added. The latter
provides a capability to define a unique identification of peer and
assures the mapping between this identification and those used in
each platform. The mapping task is assured by special wrapper. In
addition, ontology is proposed to give a clear presentation of the
structure of the P2P system without interesting in the content and the
resource managed by the peer. The ontology is created according to
the web semantic paradigm and using OWL language; so, the
structure of the system is considered as a web resource.
Abstract: Our study proposes an alternative method in building
Fuzzy Rule-Based System (FRB) from Support Vector Machine
(SVM). The first set of fuzzy IF-THEN rules is obtained through
an equivalence of the SVM decision network and the zero-ordered
Sugeno FRB type of the Adaptive Network Fuzzy Inference System
(ANFIS). The second set of rules is generated by combining the
first set based on strength of firing signals of support vectors using
Gaussian kernel. The final set of rules is then obtained from the
second set through input scatter partitioning. A distinctive advantage
of our method is the guarantee that the number of final fuzzy IFTHEN
rules is not more than the number of support vectors in the
trained SVM. The final FRB system obtained is capable of performing
classification with results comparable to its SVM counterpart, but it
has an advantage over the black-boxed SVM in that it may reveal
human comprehensible patterns.
Abstract: Ontology Matching is a task needed in various applica-tions, for example for comparison or merging purposes. In literature,many algorithms solving the matching problem can be found, butmost of them do not consider instances at all. Mappings are deter-mined by calculating the string-similarity of labels, by recognizinglinguistic word relations (synonyms, subsumptions etc.) or by ana-lyzing the (graph) structure. Due to the facts that instances are oftenmodeled within the ontology and that the set of instances describesthe meaning of the concepts better than their meta information,instances should definitely be incorporated into the matching process.In this paper several novel instance-based matching algorithms arepresented which enhance the quality of matching results obtainedwith common concept-based methods. Different kinds of formalismsare use to classify concepts on account of their instances and finallyto compare the concepts directly.KeywordsInstances, Ontology Matching, Semantic Web