Abstract: Poly-β-hydroxybutyrate (PHB) is one of the most
famous biopolymers that has various applications in production of
biodegradable carriers. The most important strategy for enhancing
efficiency in production process and reducing the price of PHB, is the
accurate expression of kinetic model of products formation and
parameters that are effective on it, such as Dry Cell Weight (DCW)
and substrate consumption. Considering the high capabilities of
artificial neural networks in modeling and simulation of non-linear
systems such as biological and chemical industries that mainly are
multivariable systems, kinetic modeling of microbial production of
PHB that is a complex and non-linear biological process, the three
layers perceptron neural network model was used in this study.
Artificial neural network educates itself and finds the hidden laws
behind the data with mapping based on experimental data, of dry cell
weight, substrate concentration as input and PHB concentration as
output. For training the network, a series of experimental data for
PHB production from Hydrogenophaga Pseudoflava by glucose
carbon source was used. After training the network, two other
experimental data sets that have not intervened in the network
education, including dry cell concentration and substrate
concentration were applied as inputs to the network, and PHB
concentration was predicted by the network. Comparison of predicted
data by network and experimental data, indicated a high precision
predicted for both fructose and whey carbon sources. Also in present
study for better understanding of the ability of neural network in
modeling of biological processes, microbial production kinetic of
PHB by Leudeking-Piret experimental equation was modeled. The
Observed result indicated an accurate prediction of PHB
concentration by artificial neural network higher than Leudeking-
Piret model.
Abstract: The aim of this study is to identify the conditions of
implementation for reconfigurability in summarizing past flexible
manufacturing systems (FMS) research by drawing overall
conclusions from many separate High Performance Manufacturing
(HPM) studies. Meta-analysis will be applied to links between HPM
programs and their practices related to FMS and manufacturing
performance with particular reference to responsiveness performance.
More specifically, an application of meta-analysis will be made with
reference to two of the main steps towards the development of an
empirically-tested theory: testing the adequacy of the measurement of
variables and testing the linkages between the variables.
Abstract: Complex assemblies of interacting proteins carry out
most of the interesting jobs in a cell, such as metabolism, DNA
synthesis, mitosis and cell division. These physiological properties
play out as a subtle molecular dance, choreographed by underlying
regulatory networks that control the activities of cyclin-dependent
kinases (CDK). The network can be modeled by a set of nonlinear
differential equations and its behavior predicted by numerical
simulation. In this paper, an innovative approach has been proposed
that uses genetic algorithms to mine a set of behavior data output by
a biological system in order to determine the kinetic parameters of
the system. In our approach, the machine learning method is
integrated with the framework of existent biological information in a
wiring diagram so that its findings are expressed in a form of system
dynamic behavior. By numerical simulations it has been illustrated
that the model is consistent with experiments and successfully shown
that such application of genetic algorithms will highly improve the
performance of mathematical model of the cell division cycle to
simulate such a complicated bio-system.
Abstract: In this paper, a robust watermarking algorithm using
the wavelet transform and edge detection is presented. The efficiency
of an image watermarking technique depends on the preservation of
visually significant information. This is attained by embedding the
watermark transparently with the maximum possible strength. The
watermark embedding process is carried over the subband
coefficients that lie on edges, where distortions are less noticeable,
with a subband level dependent strength. Also, the watermark is
embedded to selected coefficients around edges, using a different
scale factor for watermark strength, that are captured by a
morphological dilation operation. The experimental evaluation of the
proposed method shows very good results in terms of robustness and
transparency to various attacks such as median filtering, Gaussian
noise, JPEG compression and geometrical transformations.
Abstract: In this study, three strains of Vibrio parahaemolyticus
(690, BCRC 13023 and BCRC 13025) were subjected to acid
adaptation at pH 5.5 for 90 min. The survival of acid-adapted and
non-adapted V. parahaemolyticus strains under simulated gastric
condition and their protein expression profiles were investigated.
Results showed that acid adaptation increased the survival of the test
V. parahaemolyticus strains after exposure to simulated gastric juice
(pH 3). Additionally, acid adaptation also affected the protein
expression in these V. parahaemolyticus strains. Nine proteins,
identified as atpA, atpB, DnaK, GroEL, OmpU, enolase,
fructose-bisphosphate aldolase, phosphoglycerate kinase and
triosephosphate isomerase, were induced by acid adaptation in two or
three of the test strains. These acid-adaptive proteins may play
important regulatory roles in the acid tolerance response (ATR) of V.
parahaemolyticus.
Abstract: Matrix metalloproteinases (MMP) are a class of
structural and functional related enzymes involved in altering the
natural elements of the extracellular matrix. Most of the MMP
structures are cristalographycally determined and published in
WorldWide ProteinDataBank, isolated, in full structure or bound to
natural or synthetic inhibitors. This study proposes an algorithm to
replace missing crystallographic structures in PDB database. We
have compared the results of a chosen docking algorithm with a
known crystallographic structure in order to validate enzyme sites
reconstruction there where crystallographic data are missing.
Abstract: An experiment was conducted to study the effects of different types of probiotic on Sucrase enzyme activity of the small intestine mucosa in male broilers. The experimental design was arranged as randomized completely blocks in 4 × 2 factorial arrangement of treatment. 180 male broilers of Ross 308 commercial hybrid were designated into 4 groups. Three replicates of 15 birds were assigned to each treatment. Control treatments (diet contained no probiotic) were fed according to the NRC as base diet and three treatment groups were fed from the same diet plus three different types of probiotics. Birds were slaughtered after 21 and 42 days and different segments of small intestine (at 1,10,30,50,70 and 90% of total length the small intestine) were taken from each replicates (N=2) Sucrase enzyme activities were measured and recorded. Obtained data were analyzed by Spss (P
Abstract: Tomato nutrition value, color, flavor of their fruits and products depends mainly on lycopene, β-carotene, ascorbic acid, sugars and their ratio. The two most important carotenoids in fruits of tomato are lycopene, which determined fruits red color, and β- carotene, which accounts for approximately 7% of the tomato carotenoids. Therefore, tomato products and their quality can be well characterized by the content of these elements.Maturity at harvest is very important to composition and quality of tomatoes. This is especially a problem with tomatoes picked green since it is difficult to differentiate between mature and immature-green fruits. Typical and advanced mature-green tomatoes will usually attain a much better flavor than those picked at the immature or partially mature stages.To better understand the synthesis of biochemical compounds, their concentration should be compared not only at the last stage of maturity, but also during all fruit ripening period in different varieties.
Abstract: The overall objective of this research is a strain
improvement technology for efficient pectinase production. A novel
cells cultivation technology by immobilization of fungal cells has
been studied in long time continuous fermentations. Immobilization
was achieved by using of new material for absorption of stores of
immobilized cultures which was for the first time used for
immobilization of microorganisms. Effects of various conditions of
nitrogen and carbon nutrition on the biosynthesis of pectolytic
enzymes in Aspergillus awamori 1-8 strain were studied. Proposed
cultivation technology along with optimization of media components
for pectinase overproduction led to increased pectinase productivity
in Aspergillus awamori 1-8 from 7 to 8 times. Proposed technology
can be applied successfully for production of major industrial
enzymes such as α-amylase, protease, collagenase etc.
Abstract: The purpose of my research proposal is to
demonstrate that there is a relationship between EEG and
endometrial cancer.
The above relationship is based on an Aristotelian Syllogism;
since it is known that the 14-3-3 protein is related to the electrical
activity of the brain via control of the flow of Na+ and K+ ions and
since it is also known that many types of cancer are associated with
14-3-3 protein, it is possible that there is a relationship between EEG
and cancer. This research will be carried out by well-defined
diagnostic indicators, obtained via the EEG, using signal processing
procedures and pattern recognition tools such as neural networks in
order to recognize the endometrial cancer type. The current research
shall compare the findings from EEG and hysteroscopy performed on
women of a wide age range. Moreover, this practice could be
expanded to other types of cancer. The implementation of this
methodology will be completed with the creation of an ontology.
This ontology shall define the concepts existing in this research-s
domain and the relationships between them. It will represent the
types of relationships between hysteroscopy and EEG findings.
Abstract: The elimimation of mefenamic acid has been carried
out by photolysis, ozonation, adsorption onto activated carbon (AC)
and combinations of the previous single systems (O3+AC and
O3+UV). The results obtained indicate that mefenamic acid is not
photo-reactive, showing a relatively low quantum yield of the order
of 6 x 10-4 mol Einstein-1. Application of ozone to mefenamic
aqueous solutions instantaneously eliminates the pharmaceutical,
achieving simultaneously a 40% of mineralization. Addition of AC to
the ozonation process does not enhance the process, moreover,
mineralization is completely inhibited if compared to results obtained
by single ozonation. The combination of ozone and UV radiation led
to the best results in terms of mineralization (60% after 120 min).
Abstract: The halophilic proteinase showed a maximal activity
at 50°C and pH 9~10, in 20% NaCl and was highly stabilized by
NaCl. It was able to hydrolyse natural actomyosin (NAM), collagen
and anchovy protein. For NAM hydrolysis, the myosin heavy chain
was completely digested by halophilic proteinase as evidenced by the
lowest band intensity remaining, but partially hydrolysed actin. The
SR5-3 proteinase was also capable hydrolyzing two major
components of collagen, β- and α-compounds, effectively. The
degree of hydrolysis (DH) of the halophilic proteinase and
commercial proteinases (Novozyme, Neutrase, chymotrypsin and
Flavourzyme) on the anchovy protein, were compared, and it was
found that the proteinase showed a greater degree of hydrolysis
towards anchovy protein than that from commercial proteinases. DH
of halophilic proteinase was sharply enhanced according to the
increase in the concentration of enzyme from 0.035 U to 0.105 U.
The results warranting that the acceleration of the production of fish
sauce with higher quality, may be achieved by adding of the
halophilic proteinase from this bacterium.
Abstract: Nowadays for algae cell ultrasonication the
longitudinal ultrasonic piezosystems are used. In this paper a
possibility of creating unique ultrasonic piezoelectric system, which
would allow reducing energy losses and concentrating this energy to
a small closed volume are proposed. The current vibrating systems
whose ultrasonic energy is concentrated inside of hollow cylinder in
which water-algae mixture is flowing. Two, three or multiply
ultrasonic composite systems to concentrate total energy into a
hollow cylinder to creating strong algae cell ultrasonication are used.
The experiments and numerical FEM analysis results using diskshaped
transducer and the first biological test results on algae cell
disruption by ultrasonication are presented as well.
Abstract: Malting is usually carried out on intact barley seed,
while hull is still attached to it. In this study, oat grain with and
without hull was subjected to controlled germination to optimize its
enzymes activity, in such a way that lipase has the lowest and α-
amylase and proteinase the highest activities. Since pH has a great
impact on the activity of the enzymes, the pH of germination media
was set up to 3 to 8. In dehulled oats, lipase and α-amylase had the
lowest and highest activities in pHs 3 and 6, respectively whereas the
highest proteinase activity was evidenced at pH 7 and 4 in the oats
with and without hull respectively. While measurements indicated
that the effect of hull on the enzyme activities particularly in lipase
and amylase at each level of the pH are significantly different, the
best results were obtained in those samples in which their hull had
been removed. However, since the similar lipase activity in
germinated dehulled oat were recorded at the pHs 4 and 5, therefore
it was concluded that pH 5 in dehulled oat seed may provide the
optimum enzyme activity for all the enzymes.
Abstract: Hepatitis C is an infectious disease transmitted by
blood and due to hepatitis C virus (HCV), which attacks the liver.
The infection is characterized by liver inflammation (hepatitis) that is
often asymptomatic but can progress to chronic hepatitis and later
cirrhosis and liver cancer. Our problem tends to highlight on the one
hand the prevalence of infectious disease in the population of the
region of Batna and on other hand the biological characteristics of
this disease by a screening and a specific diagnosis based on
serological tests, liver checkup (measurement of haematological and
biochemical parameters).
The results showed:
The serology of hepatitis C establishes the diagnosis of infection
with hepatitis C. In this study and with the serological test, 24 cases
of the disease of hepatitis C were found in 1000 suspected cases (7
cases with normal transaminases and 17 cases with elevated
transaminases). The prevalence of this disease in this study
population was 2.4%.
The presence of hepatitis C disrupts liver function including the
onset of cytolysis, cholestasis, jaundice, thrombocytopenia, and
coagulation disorders.
Abstract: Matrix metalloproteinase-3 (MMP3) is key member
of the MMP family, and is known to be present in coronary
atherosclerotic. Several studies have demonstrated that MMP-3
5A/6A polymorphism modify each transcriptional activity in allele
specific manner. We hypothesized that this polymorphism may play
a role as risk factor for development of coronary stenosis. The aim of
our study was to estimate MMP-3 (5A/6A) gene polymorphism on
interindividual variability in risk for coronary stenosis in an Iranian
population.DNA was extracted from white blood cells and genotypes
were obtained from coronary stenosis cases (n=95) and controls
(n=100) by PCR (polymerase chain reaction) and restriction
fragment length polymorphism techniques. Significant differences
between cases and controls were observed for MMP3 genotype
frequencies (X2=199.305, p< 0.001); the 6A allele was less
frequently seen in the control group, compared to the disease group
(85.79 vs. 78%, 6A/6A+5A/6A vs. 5A/5A, P≤0.001). These data
imply the involvement of -1612 5A/6A polymorphism in coronary
stenosis, and suggest that probably the 6A/6A MMP-3 genotype is a
genetic susceptibility factor for coronary stenosis.
Abstract: Prickly pear juice has received renewed attention with regard to the effects of processing and preservation on its sensory qualities (colour, taste, flavour, aroma, astringency, visual browning and overall acceptability). Juice was prepared by homogenizing fruit and treating the pulp with pectinase (Aspergillus niger). Juice treatments applied were sugar addition, acidification, heat-treatment, refrigeration, and freezing and thawing. Prickly pear pulp and juice had unique properties (low pH 3.88, soluble solids 3.68 oBrix and high titratable acidity 0.47). Sensory profiling and descriptive analyses revealed that non-treated juice had a bitter taste with high astringency whereas treated prickly pear was significantly sweeter. All treated juices had a good sensory acceptance with values approximating or exceeding 7. Regression analysis of the consumer sensory attributes for non-treated prickly pear juice indicated an overwhelming rejection, while treated prickly pear juice received overall acceptability. Thus, educed favourable sensory responses and may have positive implications for consumer acceptability.
Abstract: In this work, ionic liquids (ILs) for CO2 capturing in typical absorption/stripper process are considered. The use of ionic liquids is considered to be cost-effective because it requires less energy for solvent recovery compared to other conventional processes. A mathematical model is developed for the process based on Peng-Robinson (PR) equation of state (EoS) which is validated with experimental data for various solutions involving CO2. The model is utilized to study the sorbent and energy demand for three types of ILs at specific CO2 capturing rates. The energy demand is manifested by the vapor-liquid equilibrium temperature necessary to remove the captured CO2 from the used solvent in the regeneration step. It is found that higher recovery temperature is required for solvents with higher solubility coefficient. For all ILs, the temperature requirement is less than that required by the typical monoethanolamine (MEA) solvent. The effect of the CO2 loading in the sorbent stream on the process performance is also examined.
Abstract: The Corporate Social Responsibility (CSR) performance has garnered significant interest during the last two decades as numerous methodologies are proposed by Social Responsible Investment (SRI) indexes. The weight of each indicator is a crucial component of the CSR measurement procedures. Based on a previous study, the appropriate weight of each proposed indicator for the Greek telecommunication sector is specified using the rank reciprocal weighting. The Kendall-s Coefficient of Concordance and Spearman Correlation Coefficient non-parametric tests are adopted to determine the level of consensus among the experts concerning the importance rank of indicators. The results show that there is no consensus regarding the rank of indicators in most of stakeholders- domains. The equal weight for all indicators could be proposed as a solution for the lack of consensus among the experts. The study recommends three different equations concerning the adopted weight approach.
Abstract: Oxyleotris marmorata is considered as
undomesticated fish, and its culture occasionally faces a problem of
food deprivation. The present study aims to evaluate alteration of
hematological indices, blood chemical associated with liver function
during 4 weeks of fasting. A non-linear relationships between fasting
days and hematological parameters (red blood cell number; y = -
0.002x2 + 0.041x + 1.249; R2=0.915, P0.05), mean corpuscular
volume; y = -0.180x2 + 2.183x + 149.61; R2=0.732, P>0.05, mean
corpuscular hemoglobin; y = -0.041x2 + 0.862x + 29.864; R2=0.818,
P>0.05 and mean corpuscular hemoglobin concentration; y = -
0.044x2 + 0.711x + 21.580; R2=0.730, P>0.05) were demonstrated.
Significant change in hematocrit (Ht) during fasting period was
observed. Ht elevated sharply increase at the first weeks of fasting
period. Higher Ht also was detected during week 2-4 of fasting time.
The significant reduction of hepatosomatic index was observed (y = -
0.007x2 - 0.096x + 1.414; R2=0.968, P0.05, serum glutamic oxaloacetic transaminase;
y = 0.005x2 – 0.201x2 + 1.297x + 33.256; R2=1, P0.05). Taken together, prolonged fasting has
deleterious effects on hematological indices, liver mass and enzyme
associated in liver function. The marked adverse effects occurred
after the first week of fasting state.