Abstract: Epidermal Growth Factor (EGF, Mw=6,045) has been
reported to have high efficiency of wound repair and anti-wrinkle
effect. However, the half-life of EGF in the body is too short to exert
the biological activity effectively when applied in free form. Growth
Factors can be stabilized by immobilization with carbohydrates from
thermal and proteolytic degradation. Low molecular weight chitosan
(LMCS) and its derivate prepared by hydrogen peroxide has high
solubility. LM6A6DC was successfully prepared as a reactive
carbohydrate for the stabilization of EGF by the reactions of LMCS
with alkalization, tosylation, azidation and reduction. The structure of
LM6A6DC was confirmed by FT-IR, 1H NMR and elementary
analysis. For enhancing the stability of free EGF, EGF was attached
with LM6A6DC by using water-soluble carbodiimide.
EGF-LM6A6DC conjugates did not show any cytotoxicity on the
Normal Human Dermal Fibroblast (NHDF) 3T3 proliferation at least
under 100 μg/ml. In the result, it was considered that LM6A6DC is
suitable to immobilize of growth factor.
Abstract: Over the past few years, the online multimedia
collection has grown at a fast pace. Several companies showed
interest to study the different ways to organise the amount of audio
information without the need of human intervention to generate
metadata. In the past few years, many applications have emerged on
the market which are capable of identifying a piece of music in a
short time. Different audio effects and degradation make it much
harder to identify the unknown piece. In this paper, an audio
fingerprinting system which makes use of a non-parametric based
algorithm is presented. Parametric analysis is also performed using
Gaussian Mixture Models (GMMs). The feature extraction methods
employed are the Mel Spectrum Coefficients and the MPEG-7 basic
descriptors. Bin numbers replaced the extracted feature coefficients
during the non-parametric modelling. The results show that nonparametric
analysis offer potential results as the ones mentioned in
the literature.
Abstract: Natural hydrocarbon seepage has helped petroleum
exploration as a direct indicator of gas and/or oil subsurface
accumulations. Surface macro-seeps are generally an indication of a
fault in an active Petroleum Seepage System belonging to a Total
Petroleum System. This paper describes a case study in which
multiple analytical techniques were used to identify and characterize
trace petroleum-related hydrocarbons and other volatile organic
compounds in groundwater samples collected from Sousse aquifer
(Central Tunisia). The analytical techniques used for analyses of
water samples included gas chromatography-mass spectrometry (GCMS),
capillary GC with flame-ionization detection, Compound
Specific Isotope Analysis, Rock Eval Pyrolysis. The objective of the
study was to confirm the presence of gasoline and other petroleum
products or other volatile organic pollutants in those samples in order
to assess the respective implication of each of the potentially
responsible parties to the contamination of the aquifer. In addition,
the degree of contamination at different depths in the aquifer was also
of interest. The oil and gas seeps have been investigated using
biomarker and stable carbon isotope analyses to perform oil-oil and
oil-source rock correlations. The seepage gases are characterized by
high CH4 content, very low δ13CCH4 values (-71,9 ‰) and high
C1/C1–5 ratios (0.95–1.0), light deuterium–hydrogen isotope ratios (-
198 ‰) and light δ13CC2 and δ13CCO2 values (-23,8‰ and-23,8‰
respectively) indicating a thermogenic origin with the contribution of
the biogenic gas. An organic geochemistry study was carried out on
the more ten oil seep samples. This study includes light hydrocarbon
and biomarkers analyses (hopanes, steranes, n-alkanes, acyclic
isoprenoids, and aromatic steroids) using GC and GC-MS. The
studied samples show at least two distinct families, suggesting two
different types of crude oil origins: the first oil seeps appears to be
highly mature, showing evidence of chemical and/or biological
degradation and was derived from a clay-rich source rock deposited
in suboxic conditions. It has been sourced mainly by the lower
Fahdene (Albian) source rocks. The second oil seeps was derived
from a carbonate-rich source rock deposited in anoxic conditions,
well correlated with the Bahloul (Cenomanian-Turonian) source rock.
Abstract: Today, the pollution due to non-degradable material
such as plastics, has led to studies about the development of
environmental-friendly material. Because of biodegradability
obtained from natural sources, polylactid acid (PLA) and ijuk fiber
are interesting to modify into a composite. This material is also
expected to reduce the impact of environmental pollution. Surface
modification of ijuk fiber through alkalinization with 0.25 M NaOH
solution for 30 minutes was aimed to enhance its compatibility to
PLA, in order to improve properties of the composite such as the
mechanical properties. Alkalinization of the ijuk fibers annihilates
some surface components such as lignin, wax and hemicelloluse, so
the pore on the surface clearly appeared, decreasing of the density
and diameter of the ijuk fibers. The change of the ijuk fiber properties
leads to increase the mechanical properties of PLA composites
reinforced the ijuk fibers through strengthening of the mechanical
interlocking with the PLA matrix. An addition to enhance the
distribution of the fibers in the PLA matrix, the stirring during DCM
solvent evaporation from the mixture of the ijuk fibers and the
dissolved-PLA can reduce amount of the trapped-voids and fibers
pull-out phenomena, which can decrease the mechanical properties of
the composite.
Abstract: This paper examines international marketing in
business practice of Czech exporting small and medium-sized
enterprises (SMEs) with regard to the strategic perspectives.
Research was focused on Czech exporting SMEs from Moravian-
Silesia region and their behavior on international markets. For
purpose of collecting data, a questionnaire was given to 262 SMEs
involved in international business. Statistics utilized in this research
included frequency, mean, percentage, and chi-square test. Data were
analyzed by Statistical Package for the Social Sciences software. The
research analysis disclosed that there is certain space for
improvement in strategic marketing especially in a marketing
research, perception of cultural and social differences, product
adaptation and usage of marketing communication tools.
Abstract: In this paper, performances of shuffled frog leaping
algorithm was investigated on the stealth laser dicing process. Effect
of problem on the performance of the algorithm was based on the
tolerance of meandering data. From the customer specification it
could be less than five microns with the target of zero microns.
Currently, the meandering levels are unsatisfactory when compared
to the customer specification. Firstly, the two-level factorial design
was applied to preliminarily study the statistically significant effects
of five process variables. In this study one influential process variable
is integer. From the experimental results, the new operating condition
from the algorithm was superior when compared to the current
manufacturing condition.
Abstract: Content Based Image Retrieval (CBIR) coupled with
Case Based Reasoning (CBR) is a paradigm that is becoming
increasingly popular in the diagnosis and therapy planning of medical
ailments utilizing the digital content of medical images. This paper
presents a survey of some of the promising approaches used in the
detection of abnormalities in retina images as well in
mammographic screening and detection of regions of interest
in MRI scans of the brain. We also describe our proposed
algorithm to detect hard exudates in fundus images of the
retina of Diabetic Retinopathy patients.
Abstract: Iron is an essential nutrient with limited
bioavailability. Nutritional anemia caused mainly by iron deficiency
is the most recognized nutritional problem in both countries as well
as affluent societies. Rice (Oryza sativa L.) has become the most
important cereal crop for the improvement of human health due to the
starch, protein, oil, and the majority of micronutrients, particularly in
Asian countries. In this study, the iron availability and profile lipid
were evaluated for the extracts from Cibeusi varieties (black rices) of
ancient rice brans.
Results: The quality of K, B, R, E diets groups shows the same
effect on the growth of rats. Hematocrit and MCHC levels of rats fed
K, B, R and E diets were not significantly (P
Abstract: This study estimates the seismic demands of tall
buildings with central symmetric setbacks by using nonlinear time
history analysis. Three setback structures, all 60-story high with
setback in three levels, are used for evaluation. The effects of
irregularities occurred by setback are evaluated by determination of
global-drift, story-displacement and story drift. Story-displacement is
modified by roof displacement and first story displacement and story
drift is modified by global drift. All results are calculated at the
center of mass and in x and y direction. Also the absolute values of
these quantities are determined. The results show that increasing of
vertical irregularities increases the global drift of the structure and
enlarges the deformations in the height of the structure. It is also
observed that the effects of geometry irregularity in the seismic
deformations of setback structures are higher than those of mass
irregularity.
Abstract: Chitosan has been an attractive biopolymer for
decades, but its processability is lowered by its poor solubility,
especially in physiological pH values. Freeze concentrated reactions
of chitosan with several organic acids including acrylic, citraconic,
itaconic, and maleic acid revealed improved solubility and
morphological properties. Solubility traits were assessed with a
modified ninhydrin test. Chitosan derivatives were characterized by
ATR-FTIR and morphological characteristics were determined by
SEM. This study is a unique approach to chemically modify chitosan
to enhance water solubility.
Abstract: Evaluated nuclear decay data for the 217Po nuclide is
presented in the present work. These data include recommended
values for the half-life T1/2, α-, β-- and γ-ray emission energies and
probabilities. Decay data from 221Rn α and 217Bi β—decays are
presented. Q(α) has been updated based on the recent published work
of the Atomic Mass Evaluation AME2012. In addition, the logft
values were calculated using the Logft program from the ENSDF
evaluation package. Moreover, the total internal conversion electrons
and the K-shell to L-shell and L-shell to M-shell and to N-shell
conversion electrons ratios K/L, L/M and L/N have been calculated
using Bricc program. Meanwhile, recommendation values or the
multi-polarities have been assigned based on recently measurement
yield a better intensity balance at the 254 keV and 264 keV gamma
transitions.
Abstract: Game theory is the study of how people interact and
make decisions to handle competitive situations. It has mainly been
developed to study decision making in complex situations. Humans
routinely alter their behaviour in response to changes in their social
and physical environment. As a consequence, the outcomes of
decisions that depend on the behaviour of multiple decision makers
are difficult to predict and require highly adaptive decision-making
strategies. In addition to the decision makers may have preferences
regarding consequences to other individuals and choose their actions
to improve or reduce the well-being of others. Nash equilibrium is a
fundamental concept in the theory of games and the most widely used
method of predicting the outcome of a strategic interaction in the
social sciences. A Nash Equilibrium exists when there is no unilateral
profitable deviation from any of the players involved. On the other
hand, no player in the game would take a different action as long as
every other player remains the same.
Abstract: Planning of infrastructure and processes in logistic
center within the frame of various kinds of logistic hubs and
technological activities in them represent quite complex problem.
The main goal is to design appropriate layout, which enables to
realize expected operation on the desired levels. The simulation
software represents progressive contemporary experimental
technique, which can support complex processes of infrastructure
planning and all of activities on it. It means that simulation
experiments, reflecting various planned infrastructure variants,
investigate and verify their eligibilities in relation with corresponding
expected operation. The inducted approach enables to make qualified
decisions about infrastructure investments or measures, which derive
benefit from simulation-based verifications. The paper represents
simulation software for simulation infrastructural layout and
technological activities in marshalling yard, intermodal terminal,
warehouse and combination between them as the parts of logistic
center.
Abstract: The aim of this paper is to select the most accurate
forecasting method for predicting the future values of the
unemployment rate in selected European countries. In order to do so,
several forecasting techniques adequate for forecasting time series
with trend component, were selected, namely: double exponential
smoothing (also known as Holt`s method) and Holt-Winters` method
which accounts for trend and seasonality. The results of the empirical
analysis showed that the optimal model for forecasting
unemployment rate in Greece was Holt-Winters` additive method. In
the case of Spain, according to MAPE, the optimal model was double
exponential smoothing model. Furthermore, for Croatia and Italy the
best forecasting model for unemployment rate was Holt-Winters`
multiplicative model, whereas in the case of Portugal the best model
to forecast unemployment rate was Double exponential smoothing
model. Our findings are in line with European Commission
unemployment rate estimates.
Abstract: Housing loans play an important role in CEE
countries’ economies. This fact is based on their share in total loans
to households and their importance for economic activity and growth
in CEE countries. Therefore, it is important to find out key
determinants of housing loans demand in these countries. The aim of
this study is to research and analyze the determinants of the demand
for housing loans in Croatia. In this regard, the effect of economic
activity, loan terms and real estate prices were analyzed. Also, the
aim of this study is to find out what motivates people to take housing
loans. Therefore, primarily empirical study was conducted among the
Croatian residents. The results show that demand for housing loans is
positively affected by economic growth, higher personal income and
flexible loan terms, while it is negatively affected by interest rate rise.
Abstract: Diminished antioxidant defense or increased
production of reactive oxygen species in the biological system can
result in oxidative stress which may lead to various
neurodegenerative diseases including Alzheimer’s disease (AD).
Microglial activation also contributes to the progression of AD by
producing several proinflammatory cytokines, nitric oxide (NO) and
prostaglandin E2 (PGE2). Oxidative stress and inflammation have
been reported to be possible pathophysiological mechanisms
underlying AD. In addition, the cholinergic hypothesis postulates that
memory impairment in patient with AD is also associated with the
deficit of cholinergic function in the brain. Although a number of
drugs have been approved for the treatment of AD, most of these
synthetic drugs have diverse side effects and yield relatively modest
benefits. Marine algae have great potential in pharmaceutical and
biomedical applications as they are valuable sources of bioactive
properties such as anticoagulation, antimicrobial, antioxidative,
anticancer and anti-inflammatory. Hence, this study aimed to provide
an overview of the properties of Malaysian seaweeds (Padina
australis, Sargassum polycystum and Caulerpa racemosa) in
inhibiting oxidative stress, neuroinflammation and cholinesterase
enzymes. These seaweeds significantly exhibited potent DPPH and
moderate superoxide anion radical scavenging ability (P
Abstract: Facing the understanding problem of update and
preservation of urban history space under background of rapid Chinese
urbanization, so at first there is a need to dig the philosophic principles
of “antithesis” and “unification” which are contained in the traditional
Chinese literature known as “antithesis” and do the job of planning
translation by personal understanding in order to form understanding
and value systems of dialectical urban history space under the
foundation of “antithesis”. Then we could put forward a “context”
concept for urban history space under the foregoing basis. After that,
we will take the update and preservation of Ningbo Tianyi Pavilion’s
historical district as an example to discuss problems related to
understanding of urban history area under the basis of Chinese
tradition culture, improvement of value system, construction of urban
trait space and Chinese “localization” of planning theory.
Abstract: Due to the rapid increase of Internet, web opinion
sources dynamically emerge which is useful for both potential
customers and product manufacturers for prediction and decision
purposes. These are the user generated contents written in natural
languages and are unstructured-free-texts scheme. Therefore, opinion
mining techniques become popular to automatically process customer
reviews for extracting product features and user opinions expressed
over them. Since customer reviews may contain both opinionated and
factual sentences, a supervised machine learning technique applies
for subjectivity classification to improve the mining performance. In
this paper, we dedicate our work is the task of opinion
summarization. Therefore, product feature and opinion extraction is
critical to opinion summarization, because its effectiveness
significantly affects the identification of semantic relationships. The
polarity and numeric score of all the features are determined by
Senti-WordNet Lexicon. The problem of opinion summarization
refers how to relate the opinion words with respect to a certain
feature. Probabilistic based model of supervised learning will
improve the result that is more flexible and effective.
Abstract: Quantification of cardiac function is performed by
calculating blood volume and ejection fraction in routine clinical
practice. However, these works have been performed by manual
contouring, which requires computational costs and varies on the
observer. In this paper, an automatic left ventricle segmentation
algorithm on cardiac magnetic resonance images (MRI) is presented.
Using knowledge on cardiac MRI, a K-mean clustering technique is
applied to segment blood region on a coil-sensitivity corrected image.
Then, a graph searching technique is used to correct segmentation
errors from coil distortion and noises. Finally, blood volume and
ejection fraction are calculated. Using cardiac MRI from 15 subjects,
the presented algorithm is tested and compared with manual
contouring by experts to show outstanding performance.
Abstract: In this research paper were investigated the main
regularities of a radical bromination reaction of decalin. There had
been studied the temperature effect, durations of reaction, frequency
rate of process, a ratio of initial components, type and number of the
initiator on decalin bromination degree.
There were specified optimum conditions of synthesis of a
perbromodecalin by the method of a decalin bromination. There are
developed the technological flowchart of receiving a
perbromodecalin and the mass balance of process on the first and the
subsequent loadings of components.
The results of research of antibacterial and antifungal activity of
synthesized bromoderivatives have been represented.