Abstract: A quartz crystal microbalance (QCM) nanosensor was developed to detect lysozyme enzyme by functionalizing its gold surface with the attachment of poly(methacroyl-L-phenylalanine) (PMAPA) nanoparticles. PMAPA was chosen as a hydrophobic matrix. The hydrophobic nanoparticles were synthesized by micro-emulsion polymerization method. Hydrophobic QCM nanosensor was tested for real time detection of lysozyme enzyme from aqueous solution. The kinetic and affinity studies were determined by using lysozyme solutions with different concentrations. The responses related with mass (Δm) and frequency (Δf) shifts were used to evaluate adsorption properties.
Abstract: This paper proposes a mathematical model and
examines the performance of an exact algorithm for a location–
transportation problems in humanitarian relief. The model determines
the number and location of distribution centers in a relief network,
the amount of relief supplies to be stocked at each distribution center
and the vehicles to take the supplies to meet the needs of disaster
victims under capacity restriction, transportation and budgetary
constraints. The computational experiments are conducted on the
various sizes of problems that are generated. Branch and bound
algorithm is applied for these problems. The results show that this
algorithm can solve problem sizes of up to three candidate locations
with five demand points and one candidate location with up to twenty
demand points without premature termination.
Abstract: Waxapple (Syzygium samarngense Merr.et Perry) is an
important tropical fruit in Taiwan. The famous producing area is
located on the coast in Pingtung County. Land subsidence and climate
change will tend to soil alkalization more seriously. This study was to
evaluate the effects of NaCl in waxapple seedlings. NaCl salinity
reduced waxapple shoot growth; it may due to reducing relative water
content in leaf and new shoot. Leaf Cl and Na concentration were
increased but K, Ca, and Mg content had no significant difference after
irrigated with NaCl for six weeks. In roots, Na and Cl content increase
significantly with 90 mM NaCl treatment, but K, Ca, and Mg content
was reduced. 30-90mM Nacl treatment do not effect K/Na, Ca/Na and
Mg/Na ratio, but decrease significantly in 90mM treatment in roots.
The leaf and root electrolyte leakage were significantly affected by 90
mM NaCl treatment. Suggesting 90mM was optimum concentration
for sieve out other tolerance waxapples verities.
Abstract: In this study, we have focused our attention on
combining of molecular imprinting into nanofilms and QCM
nanosensor approaches and producing QCM nanosensor for anti-
CCP, chosen as model protein, using anti-CCP imprinted nanofilms.
The nonimprinted nanosensor was also prepared to evaluate the
selectivity of the imprinted nanosensor. Anti-CCP imprinted QCM
nanosensor was tested for real time detection of anti-CCP from
aqueous solution. The kinetic and affinity studies were determined by
using anti-CCP solutions with different concentrations. The
responses related with mass shifts (%m) and frequency shifts (%f)
were used to evaluate adsorption properties. To show the selectivity
of the anti-CCP imprinted QCM nanosensor, competitive adsorption
of anti-CCP and IgM was investigated. The results indicate that anti-
CCP imprinted QCM nanosensor has higher adsorption capabilities
for anti-CCP than for IgM, due to selective cavities in the polymer
structure.
Abstract: This study examines analytically the effect of tsunami loads on reinforced concrete (RC) frame buildings. The impact of tsunami wave loads and waterborne objects are analyzed using a typical substandard full-scale two-story RC frame building tested as part of the EU-funded Ecoleader project. The building was subjected to shake table tests in bare condition, and subsequently strengthened using Carbon Fiber Reinforced Polymers (CFRP) composites and retested. Numerical models of the building in both bare and CFRP-strengthened conditions are calibrated in DRAIN-3DX software to match the test results. To investigate the response of wave loads and impact forces, the numerical models are subjected to nonlinear dynamic analyses using force time-history input records. The analytical results are compared in terms of displacements at the floors and at the “impact point” of a boat. The results show that the roof displacement of the CFRP-strengthened building reduced by 63% when compared to the bare building. The results also indicate that strengthening only the mid-height of the impact column using CFRP is more effective at reducing damage when compared to strengthening other parts of the column. Alternative solutions to mitigate damage due to tsunami loads are suggested.
Abstract: Biofuels production has come forth as a future
technology to combat the problem of depleting fossil fuels. Bio-based
ethanol production from enzymatic lignocellulosic biomass
degradation serves an efficient method and catching the eye of
scientific community. High cost of the enzyme is the major obstacle
in preventing the commercialization of this process. Thus main
objective of the present study was to optimize composition of
medium components for enhancing cellulase production by newly
isolated strain of Bacillus tequilensis. Nineteen factors were taken
into account using statistical Plackett-Burman Design. The significant
variables influencing the cellulose production were further employed
in statistical Response Surface Methodology using Central
Composite Design for maximizing cellulase production. The
optimum medium composition for cellulase production was: peptone
(4.94 g/L), ammonium chloride (4.99 g/L), yeast extract (2.00 g/L),
Tween-20 (0.53 g/L), calcium chloride (0.20 g/L) and cobalt chloride
(0.60 g/L) with pH 7, agitation speed 150 rpm and 72 h incubation at
37oC. Analysis of variance (ANOVA) revealed high coefficient of
determination (R2) of 0.99. Maximum cellulase productivity of 11.5
IU/ml was observed against the model predicted value of 13 IU/ml.
This was found to be optimally active at 60oC and pH 5.5.
Abstract: The aim of this paper is to perform experimental
modal analysis (EMA) of reinforced concrete (RC) square slabs.
EMA is the process of determining the modal parameters (Natural
Frequencies, damping factors, modal vectors) of a structure from a
set of frequency response functions FRFs (curve fitting). Although,
experimental modal analysis (or modal testing) has grown steadily in
popularity since the advent of the digital FFT spectrum analyzer in
the early 1970’s, studying all types of members and materials using
such method have not yet been well documented. Therefore, in this
work, experimental tests were conducted on RC square slab
specimens of dimensions 600mm x 600mmx 40mm. Experimental
analysis was based on freely supported boundary condition.
Moreover, impact testing as a fast and economical means of finding
the modes of vibration of a structure was used during the
experiments. In addition, Pico Scope 6 device and MATLAB
software were used to acquire data, analyze and plot Frequency
Response Function (FRF). The experimental natural frequencies
which were extracted from measurements exhibit good agreement
with analytical predictions. It is showed that EMA method can be
usefully employed to investigate the dynamic behavior of RC slabs.
Abstract: As the use of geothermal energy grows internationally
more effort is required to monitor and protect areas with rare and
important geothermal surface features. A number of approaches are
presented for developing and calibrating numerical geothermal
reservoir models that are capable of accurately representing
geothermal surface features. The approaches are discussed in the
context of cases studies of the Rotorua geothermal system and the
Orakei-korako geothermal system, both of which contain important
surface features. The results show that models are able to match the
available field data accurately and hence can be used as valuable
tools for predicting the future response of the systems to changes in
use.
Abstract: Elastomeric dielectric material has recently become a
new alternative for actuator technology. The characteristics of
dielectric elastomers placed between two electrodes to withstand
large strain when electrodes are charged has attracted the attention of
many researcher to study this material for actuator technology. Thus,
in the past few years Danfoss Ventures A/S has established their own
dielectric electro-active polymer (DEAP), which was called
PolyPower.
The main objective of this work was to investigate the dynamic
characteristics for vibration control of a PolyPower actuator folded in
‘pull’ configuration. A range of experiments was carried out on the
folded actuator including passive (without electrical load) and active
(with electrical load) testing. For both categories static and dynamic
testing have been done to determine the behavior of folded DEAP
actuator.
Voltage-Strain experiments show that the DEAP folded actuator is
a non-linear system. It is also shown that the voltage supplied has no
effect on the natural frequency. Finally, varying AC voltage with
different amplitude and frequency shows the parameters that
influence the performance of DEAP folded actuator. As a result, the
actuator performance dominated by the frequency dependence of the
elastic response and was less influenced by dielectric properties.
Abstract: Geometric and mechanical properties all influence the
resistance of RC structures and may, in certain combination of
property values, increase the risk of a brittle failure of the whole
system.
This paper presents a statistical and probabilistic investigation on
the resistance of RC beams designed according to Eurocodes 2 and 8,
and subjected to multiple failure modes, under both the natural
variation of material properties and the uncertainty associated with
cross-section and transverse reinforcement geometry. A full
probabilistic model based on JCSS Probabilistic Model Code is
derived. Different beams are studied through material nonlinear
analysis via Monte Carlo simulations. The resistance model is
consistent with Eurocode 2. Both a multivariate statistical evaluation
and the data clustering analysis of outcomes are then performed.
Results show that the ultimate load behaviour of RC beams
subjected to flexural and shear failure modes seems to be mainly
influenced by the combination of the mechanical properties of both
longitudinal reinforcement and stirrups, and the tensile strength of
concrete, of which the latter appears to affect the overall response of
the system in a nonlinear way. The model uncertainty of the
resistance model used in the analysis plays undoubtedly an important
role in interpreting results.
Abstract: A knowledge-based expert system with the acronym
RASPE is developed as an application tool to help decision makers in
construction companies make informed decisions about managing
risks in pipeline construction projects. Choosing to use expert
systems from all available artificial intelligence techniques is due to
the fact that an expert system is more suited to representing a
domain’s knowledge and the reasoning behind domain-specific
decisions. The knowledge-based expert system can capture the
knowledge in the form of conditional rules which represent various
project scenarios and potential risk mitigation/response actions. The
built knowledge in RASPE is utilized through the underlying
inference engine that allows the firing of rules relevant to a project
scenario into consideration. Paper provides an overview of the
knowledge acquisition process and goes about describing the
knowledge structure which is divided up into four major modules.
The paper shows one module in full detail for illustration purposes
and concludes with insightful remarks.
Abstract: A collection of thirty cultivars/clones of a red pitaya
was used to investigate flowering response to lighting
supplementation in the winter season of 2013-2014 in southern
Taiwan. The night-breaking treatment was conducted during the
period of 10 Oct. 2013 to 5 Mar. 2014 with 4-continuous hours
(22.00 – 02.00 hrs) of additional lighting daily using incandescent
bulbs (100W). Among cultivars and clones tested, twenty-three
genotypes, most belonging to the red-magenta flesh type, were found
to have positively flowering response to the lighting treatment. The
duration of night-breaking treatment for successful flowering
initiation varied from 33- 48 days. The lighting-sensitive genotypes
bore 1-2 flowering flushes. Floral and fruiting stages took 21-26 and
46-59 days, respectively. Among sixteen fruiting genotypes, the
highest fruit set rates were found in Damao 9, D4, D13, Chaozou
large, Chaozhou 5, Small Nick and F22. Five cultivars and clones
(Orejona, D4, Chaozhou large, Chaozhou 5 and Small Nick) produced
fruits with an average weight of more than 300 g per fruit which were
higher than those of the fruits formed in the summer of 2013. Fruits
produced during off-season containing total soluble solids (TSS)
from 17.5 to 20.7oBrix, which were higher than those produced inseason.
Abstract: This study is concerned with the optimization of
fermentation parameters for the hyper production of mannanase from
Fusarium oxysporum SS-25 employing two step statistical strategy
and kinetic characterization of crude enzyme preparation. The
Plackett-Burman design used to screen out the important factors in
the culture medium revealed 20% (w/w) wheat bran, 2% (w/w) each
of potato peels, soyabean meal and malt extract, 1% tryptone, 0.14%
NH4SO4, 0.2% KH2PO4, 0.0002% ZnSO4, 0.0005% FeSO4, 0.01%
MnSO4, 0.012% SDS, 0.03% NH4Cl, 0.1% NaNO3 in brewer’s spent
grain based medium with 50% moisture content, inoculated with
2.8×107 spores and incubated at 30oC for 6 days to be the main
parameters influencing the enzyme production. Of these factors, four
variables including soyabean meal, FeSO4, MnSO4 and NaNO3 were
chosen to study the interactive effects and their optimum levels in
central composite design of response surface methodology with the
final mannanase yield of 193 IU/gds. The kinetic characterization
revealed the crude enzyme to be active over broader temperature and
pH range. This could result in 26.6% reduction in kappa number with
4.93% higher tear index and 1% increase in brightness when used to
treat the wheat straw based kraft pulp. The hydrolytic potential of
enzyme was also demonstrated on both locust bean gum and guar
gum.
Abstract: Different tools and technologies were implemented
for Crisis Response and Management (CRM) which is generally
using available network infrastructure for information exchange.
Depending on type of disaster or crisis, network infrastructure could
be affected and it could not be able to provide reliable connectivity.
Thus any tool or technology that depends on the connectivity could
not be able to fulfill its functionalities. As a solution, a new message
exchange framework has been developed. Framework provides
offline/online information exchange platform for CRM Information
Systems (CRMIS) and it uses XML compression and packet
prioritization algorithms and is based on open source web
technologies. By introducing offline capabilities to the web
technologies, framework will be able to perform message exchange
on unreliable networks. The experiments done on the simulation
environment provide promising results on low bandwidth networks
(56kbps and 28.8 kbps) with up to 50% packet loss and the solution is
to successfully transfer all the information on these low quality
networks where the traditional 2 and 3 tier applications failed.
Abstract: Physics Education Research (PER) results have shown
that students do not achieve the expected level of competency in
understanding the concepts of different domains of Physics learning
when taught by the traditional teaching methods, the concepts of
Electricity and Magnetism (E&M) being one among them.
Simulation being one of the valuable instructional tools renders an
opportunity to visualize varied experiences with such concepts.
Considering the electric force concept which requires extensive use
of vector representations, we report here the outcome of the research
results pertaining to the student understanding of this concept and the
role of simulation in using vector representation. The simulation
platform provides a positive impact on the use of vector
representation.
The first stage of this study involves eliciting and analyzing
student responses to questions that probe their understanding of the
concept of electrostatic force and this is followed by four stages of
student interviews as they use the interactive simulations of electric
force in one dimension. Student responses to the questions are
recorded in real time using electronic pad. A validation test interview
is conducted to evaluate students' understanding of the electric force
concept after using interactive simulation. Results indicate lack of
procedural knowledge of the vector representation. The study
emphasizes the need for the choice of appropriate simulation and
mode of induction for learning.
Abstract: Building loss estimation methodologies which have
been advanced considerably in recent decades are usually used to
estimate socio and economic impacts resulting from seismic structural
damage. In accordance with these methods, this paper presents the
evaluation of an annual loss probability of a reinforced concrete
moment resisting frame designed according to Korean Building Code.
The annual loss probability is defined by (1) a fragility curve obtained
from a capacity spectrum method which is similar to a method adopted
from HAZUS, and (2) a seismic hazard curve derived from annual
frequencies of exceedance per peak ground acceleration. Seismic
fragilities are computed to calculate the annual loss probability of a
certain structure using functions depending on structural capacity,
seismic demand, structural response and the probability of exceeding
damage state thresholds. This study carried out a nonlinear static
analysis to obtain the capacity of a RC moment resisting frame
selected as a prototype building. The analysis results show that the
probability of being extensive structural damage in the prototype
building is expected to 0.01% in a year.
Abstract: In this paper, model order reduction method is used
for approximation in linear and nonlinearity aspects in some
experimental data. This method can be used for obtaining offline
reduced model for approximation of experimental data and can
produce and follow the data and order of system and also it can
match to experimental data in some frequency ratios. In this study,
the method is compared in different experimental data and influence
of choosing of order of the model reduction for obtaining the best and
sufficient matching condition for following the data is investigated in
format of imaginary and reality part of the frequency response curve
and finally the effect and important parameter of number of order
reduction in nonlinear experimental data is explained further.
Abstract: An existing RC building in Madinah is seismically
evaluated with and without infill wall. Four model systems have been
considered i.e. model I (no infill), model IIA (strut infill-update from
field test), model IIB (strut infill- ASCE/SEI 41) and model IIC (strut
infill-Soft storey- ASCE/SEI 41). Three dimensional pushover
analyses have been carried out using SAP2000 software
incorporating inelastic material behavior for concrete, steel and infill
walls. Infill wall has been modeled as equivalent strut according to
suggested equation matching field test measurements and to the
ASCE/SEI 41 equation. The effect of building modeling on the
performance point as well as capacity and demand spectra due to EQ
design spectrum function in Madinah area has been investigated. The
response modification factor (R) for the 5 story RC building is
evaluated from capacity and demand spectra (ATC-40) for the
studied models. The results are summarized and discussed.
Abstract: A capacity spectrum method (CSM), one of methodologies to evaluate seismic fragilities of building structures, has been long recognized as the most convenient method, even if it contains several limitations to predict the seismic response of structures of interest. This paper proposes the procedure to estimate seismic fragility curves using an incremental dynamic analysis (IDA) rather than the method adopting a CSM. To achieve the research purpose, this study compares the seismic fragility curves of a 5-story reinforced concrete (RC) moment frame obtained from both methods; an IDA method and aCSM. Both seismic fragility curves are similar in slight and moderate damage states whereas the fragility curve obtained from the IDA method presents less variation (or uncertainties) in extensive and complete damage states. This is due to the fact that the IDA method can properly capture the structural response beyond yielding rather than the CSM and can directly calculate higher mode effects. From these observations, the CSM could overestimate seismic vulnerabilities of the studied structure in extensive or complete damage states.
Abstract: The present study was carried out to evaluate the
effect of different levels of dietary seed and extract of Harmal
(Peganum harmala L.) on immunity of broiler chicks. A total of 350
one-day old broiler chicks (Ross 308) were randomly allocated to
five dietary treatments with four replicates pen of 14 birds each.
Dietary treatments consisted of control, 1 and 2 g/kg Harmal seed in
diet, 100 and 200 mg/L Harmal seed extract in water. Broilers
received dietary treatments from 1 to 42 d. Two birds from each pen
were randomly weighed and sacrificed at 42 d of age, the relative
weight of lymphoid organs (bursa of Fabercius and spleen) to live
weight were calculated. Antibody titers against Newcastle and
influenza viruses and sheep red blood cell were measured at 30 d of
age. Results showed that the relative weights of lymphoid organs
were not affected by dietary treatments. Furthermore, antibody titer
against Newcastle and influenza viruses as well as sheep red blood
cell antigen were significantly (P