Abstract: As in other countries from Central and Eastern Europe,
the economic restructuring occurred in the last decade of the
twentieth century affected the mining industry in Romania, an
oversize and heavily subsidized sector before 1989. After more than
a decade since the beginning of mining restructuring, an evaluation
of current social implications of the process it is required, together
with an efficiency analysis of the adaptation mechanisms developed
at governmental level. This article aims to provide an insight into
these issues through case studies conducted in the most important
coal basin of Romania, Petroşani Depression.
Abstract: In the current research, neuro-fuzzy model and regression model was developed to predict Material Removal Rate in Electrical Discharge Machining process for AISI D2 tool steel with copper electrode. Extensive experiments were conducted with various levels of discharge current, pulse duration and duty cycle. The experimental data are split into two sets, one for training and the other for validation of the model. The training data were used to develop the above models and the test data, which was not used earlier to develop these models were used for validation the models. Subsequently, the models are compared. It was found that the predicted and experimental results were in good agreement and the coefficients of correlation were found to be 0.999 and 0.974 for neuro fuzzy and regression model respectively
Abstract: The major objective of this paper is to introduce a new method to select genes from DNA microarray data. As criterion to select genes we suggest to measure the local changes in the correlation graph of each gene and to select those genes whose local changes are largest. More precisely, we calculate the correlation networks from DNA microarray data of cervical cancer whereas each network represents a tissue of a certain tumor stage and each node in the network represents a gene. From these networks we extract one tree for each gene by a local decomposition of the correlation network. The interpretation of a tree is that it represents the n-nearest neighbor genes on the n-th level of a tree, measured by the Dijkstra distance, and, hence, gives the local embedding of a gene within the correlation network. For the obtained trees we measure the pairwise similarity between trees rooted by the same gene from normal to cancerous tissues. This evaluates the modification of the tree topology due to tumor progression. Finally, we rank the obtained similarity values from all tissue comparisons and select the top ranked genes. For these genes the local neighborhood in the correlation networks changes most between normal and cancerous tissues. As a result we find that the top ranked genes are candidates suspected to be involved in tumor growth. This indicates that our method captures essential information from the underlying DNA microarray data of cervical cancer.
Abstract: Mycophenolic acid “MPA" is a secondary metabolite
of Penicillium bervicompactum with antibiotic and
immunosuppressive properties. In this study, fermentation process
was established for production of mycophenolic acid by Penicillium
bervicompactum MUCL 19011 in shake flask. The maximum MPA
production, product yield and productivity were 1.379 g/L, 18.6 mg/g
glucose and 4.9 mg/L.h respectively. Glucose consumption, biomass
and MPA production profiles were investigated during fermentation
time. It was found that MPA production starts approximately after
180 hours and reaches to a maximum at 280 h. In the next step, the
effects of methionine and acetate concentrations on MPA production
were evaluated. Maximum MPA production, product yield and
productivity (1.763 g/L, 23.8 mg/g glucose and 6.30 mg/L. h
respectively) were obtained with using 2.5 g/L methionine in culture
medium. Further addition of methionine had not more positive effect
on MPA production. Finally, results showed that the addition of
acetate to the culture medium had not any observable effect on MPA
production
Abstract: The main aim of this research is to study the possible
use of recycled fine aggregate made from waste rubble wall to
substitute partially for the natural sand used in the production of
cement and sand bricks. The bricks specimens were prepared by
using 100% natural sand; they were then replaced by recycled fine
aggregate at 25, 50, 75, and 100% by weight of natural sand. A series
of tests was carried out to study the effect of using recycled aggregate
on the physical and mechanical properties of bricks, such as density,
drying shrinkage, water absorption characteristic, compressive and
flexural strength. Test results indicate that it is possible to
manufacture bricks containing recycled fine aggregate with good
characteristics that are similar in physical and mechanical properties
to those of bricks with natural aggregate, provided that the percentage
of recycled fine aggregates is limited up to 50-75%.
Abstract: This paper aims to discuss the influence of resistance
characteristic on the high conductive concrete considering the changes
of voltage and environment. The high conductive concrete with
appropriate proportion is produced to the press-electrode method. The
curve of resistivity with the changes of voltage and environment is
plotted and the changes of resistivity are explored.
Abstract: Basic objective of this study is to create a regression
analysis method that can estimate the length of a plastic hinge which
is an important design parameter, by making use of the outcomes of
(lateral load-lateral displacement hysteretic curves) the experimental
studies conducted for the reinforced square concrete columns. For
this aim, 170 different square reinforced concrete column tests results
have been collected from the existing literature. The parameters
which are thought affecting the plastic hinge length such as crosssection
properties, features of material used, axial loading level,
confinement of the column, longitudinal reinforcement bars in the
columns etc. have been obtained from these 170 different square
reinforced concrete column tests. In the study, when determining the
length of plastic hinge, using the experimental test results, a
regression analysis have been separately tested and compared with
each other. In addition, the outcome of mentioned methods on
determination of plastic hinge length of the reinforced concrete
columns has been compared to other methods available in the
literature.
Abstract: A new concept for long-term reagent storage for Labon- a-Chip (LoC) devices is described. Here we present a polymer multilayer stack with integrated stick packs for long-term storage of several liquid reagents, which are necessary for many diagnostic applications. Stick packs are widely used in packaging industry for storing solids and liquids for long time. The storage concept fulfills two main requirements: First, a long-term storage of reagents in stick packs without significant losses and interaction with surroundings, second, on demand releasing of liquids, which is realized by pushing a membrane against the stick pack through pneumatic pressure. This concept enables long-term on-chip storage of liquid reagents at room temperature and allows an easy implementation in different LoC devices.
Abstract: The purpose of research was to know the role of
immunogenic protein of 49 kDa from V.alginolyticus which capable
to initiate molecule expression of MHC Class II in receptor of
Cromileptes altivelis. The method used was in vivo experimental
research through testing of immunogenic protein 49 kDa from
V.alginolyticus at Cromileptes altivelis (size of 250 - 300 grams)
using 3 times booster by injecting an immunogenic protein in a
intramuscular manner. Response of expressed MHC molecule was
shown using immunocytochemistry method and SEM. Results
indicated that adhesin V.alginolyticus 49 kDa which have
immunogenic character could trigger expression of MHC class II on
receptor of grouper and has been proven by staining using
immunocytochemistry and SEM with labeling using antibody anti
MHC (anti mouse). This visible expression based on binding between
epitopes antigen and antibody anti MHC in the receptor. Using
immunocytochemistry, intracellular response of MHC to in vivo
induction of immunogenic adhesin from V.alginolyticus was shown.
Abstract: Analytical expression for maximum power transfer
through a transmission line limited by voltage stability has been
formulated using exact representation of transmission line with
ABCD parameters. The expression has been used for plotting PV
curve at different power factors of a radial transmission line.
Limiting values of reactive power have been obtained.
Abstract: Day by day technology increases and problems
associated with this technology also increase. Several researches
were carried out to investigate the deployment of such material safely
in geotechnical engineering in particular and civil engineering in
general. However, different types of waste material have such as
cement duct, fly ash and slag been proven to be suitable in several
applications. In this research cement dust mixed with different
percentages of sand will be used in some civil engineering
application as will be explained later in this paper throughout filed
and laboratory test. The used mixer (waste material with sand) prove
high performance, durability to environmental condition, low cost
and high benefits. At higher cement dust ratio, small cement ratio is
valuable for compressive strength and permeability. Also at small
cement dust ratio higher cement ratio is valuable for compressive
strength.
Abstract: The cinema in Turkey during the 1940s was shaped
under the Second World War conditions. The amateur film makers
from different socioeconomic roots experienced movie production in
those years. Having similar socioeconomic characteristics and
autobiographies, each of them has a different understanding of
cinema. Nevertheless, they joined in making movies which address
native culture and audience. They narrated indigenous stories with
native music, amateur players and simple settings. Although the
martial law, censorship and economical deficiencies, they started to
produce films in the Second World War. The cinematographers of the
1940s usually called as thetransition period cinematographers in
Turkey, producing in the passage between the period of thetheatre
playersand the period of thenational cinema. But, 1940- 1950 period
of Turkish cinema should be defined not as a transition but a period
of forming the professional conscioussness in cinema.
Abstract: Digital watermarking in medical images can ensure
the authenticity and integrity of the image. This design paper reviews
some existing watermarking schemes and proposes a reversible
tamper detection and recovery watermarking scheme. Watermark
data from ROI (Region Of Interest) are stored in RONI (Region Of
Non Interest). The embedded watermark allows tampering detection
and tampered image recovery. The watermark is also reversible and
data compression technique was used to allow higher embedding
capacity.
Abstract: Detection and tracking of the lip contour is an important
issue in speechreading. While there are solutions for lip tracking
once a good contour initialization in the first frame is available,
the problem of finding such a good initialization is not yet solved
automatically, but done manually. We have developed a new tracking
solution for lip contour detection using only few landmarks (15
to 25) and applying the well known Active Shape Models (ASM).
The proposed method is a new LMS-like adaptive scheme based on
an Auto regressive (AR) model that has been fit on the landmark
variations in successive video frames. Moreover, we propose an extra
motion compensation model to address more general cases in lip
tracking. Computer simulations demonstrate a fair match between
the true and the estimated spatial pixels. Significant improvements
related to the well known LMS approach has been obtained via a
defined Frobenius norm index.
Abstract: In this paper we present an adaptive method for image
compression that is based on complexity level of the image. The
basic compressor/de-compressor structure of this method is a multilayer
perceptron artificial neural network. In adaptive approach
different Back-Propagation artificial neural networks are used as
compressor and de-compressor and this is done by dividing the
image into blocks, computing the complexity of each block and then
selecting one network for each block according to its complexity
value. Three complexity measure methods, called Entropy, Activity
and Pattern-based are used to determine the level of complexity in
image blocks and their ability in complexity estimation are evaluated
and compared. In training and evaluation, each image block is
assigned to a network based on its complexity value. Best-SNR is
another alternative in selecting compressor network for image blocks
in evolution phase which chooses one of the trained networks such
that results best SNR in compressing the input image block. In our
evaluations, best results are obtained when overlapping the blocks is
allowed and choosing the networks in compressor is based on the
Best-SNR. In this case, the results demonstrate superiority of this
method comparing with previous similar works and JPEG standard
coding.
Abstract: In this paper, position vector of a partially null unit speed curve with respect to standard frame of Minkowski space-time is studied. First, it is proven that position vector of every partially null unit speed curve satisfies a vector differential equation of fourth order. In terms of solution of the differential equation, position vector of a partially null unit speed curve is expressed.
Abstract: Multifunctional structures are a potentially disruptive
technology that allows for significant mass savings on spacecraft.
The specific concept addressed herein is that of a multifunctional
power structure. In this paper, a parametric optimisation of the
design of such a structure that uses commercially available battery
cells is presented. Using numerical modelling, it was found that there
exists several trade-offs aboutthe conflict between the capacity of the
panel and its mechanical properties. It was found that there is no
universal optimal location for the cells. Placing them close to the
mechanical interfaces increases loading in the mechanically weak
cells whereas placing them at the centre of the panel increases the
stress inthe panel and reduces the stiffness of the structure.
Abstract: β-Glucosidase is an important enzyme for production
of ethanol from lignocellulose. With hydrolytic activity on
cellooligosaccharides, especially cellobiose, β-glucosidase removes
product inhibitory effect on cellulases and forms fermentable sugars.
In this study, β-glucosidase encoding gene (BGL1) from traditional
starter yeast Saccharomycosis fibuligera BMQ908 was cloned and
expressed in Pichia pastoris. BGL1 of S. fibuligera BMQ 908 shared
98% nucleotide homology with the closest GenBank sequence
(M22475) but identity in amino-acid sequences of catalytic domains.
Recombinant plasmid pPICZαA/BGL1 containing the sequence
encoding BGL1 mature protein and α-factor secretion signal was
constructed and transformed into methylotrophic yeast P. pastoris by
electroporation. The recombinant strain produced single extracellular
protein with molecular weight of 120 kDa and cellobiase activity of
60 IU/ml. The optimum pH of the recombinant β-glucosidase was 5.0
and the optimum temperature was 50°C.
Abstract: This paper presents a complete procedure for tool path
planning and blade machining in 5-axis manufacturing. The actual
cutting contact and cutter locations can be determined by lead and tilt
angles. The tool path generation is implemented by piecewise curved
approximation and chordal deviation detection. An application about
drive surface method promotes flexibility of tool control and stability
of machine motion. A real manufacturing process is proposed to
separate the operation into three regions with five stages and to modify
the local tool orientation with an interactive algorithm.
Abstract: The transformation of vocal characteristics aims at
modifying voice such that the intelligibility of aphonic voice is
increased or the voice characteristics of a speaker (source speaker) to
be perceived as if another speaker (target speaker) had uttered it. In
this paper, the current state-of-the-art voice characteristics
transformation methodology is reviewed. Special emphasis is placed
on voice transformation methodology and issues for improving the
transformed speech quality in intelligibility and naturalness are
discussed. In particular, it is suggested to use the modulation theory
of speech as a base for research on high quality voice transformation.
This approach allows one to separate linguistic, expressive, organic
and perspective information of speech, based on an analysis of how
they are fused when speech is produced. Therefore, this theory
provides the fundamentals not only for manipulating non-linguistic,
extra-/paralinguistic and intra-linguistic variables for voice
transformation, but also for paving the way for easily transposing the
existing voice transformation methods to emotion-related voice
quality transformation and speaking style transformation. From the
perspectives of human speech production and perception, the popular
voice transformation techniques are described and classified them
based on the underlying principles either from the speech production
or perception mechanisms or from both. In addition, the advantages
and limitations of voice transformation techniques and the
experimental manipulation of vocal cues are discussed through
examples from past and present research. Finally, a conclusion and
road map are pointed out for more natural voice transformation
algorithms in the future.