The Role of Immunogenic Adhesin Vibrio alginolyticus 49 k Da to Molecule Expression of Major Histocompatibility Complex on Receptors of Humpback Grouper Cromileptes altivelis
The purpose of research was to know the role of
immunogenic protein of 49 kDa from V.alginolyticus which capable
to initiate molecule expression of MHC Class II in receptor of
Cromileptes altivelis. The method used was in vivo experimental
research through testing of immunogenic protein 49 kDa from
V.alginolyticus at Cromileptes altivelis (size of 250 - 300 grams)
using 3 times booster by injecting an immunogenic protein in a
intramuscular manner. Response of expressed MHC molecule was
shown using immunocytochemistry method and SEM. Results
indicated that adhesin V.alginolyticus 49 kDa which have
immunogenic character could trigger expression of MHC class II on
receptor of grouper and has been proven by staining using
immunocytochemistry and SEM with labeling using antibody anti
MHC (anti mouse). This visible expression based on binding between
epitopes antigen and antibody anti MHC in the receptor. Using
immunocytochemistry, intracellular response of MHC to in vivo
induction of immunogenic adhesin from V.alginolyticus was shown.
[1] K. Yuasa, Des Roza, I. Koesharyani, F. Johnny and K. Mahardika.
General Remarks On Fish Disease Diagnosis. Textbook for the Training
Course on Fish Disease Diagnosis. Lolitkanta-JICA Booklet No. 12.
2000, pp. 5-18.
[2] U. Yanuhar. Development of Vaccine based on Receptor Peptide of
Grouper recognizing Antigen as Transgenic Antibody for Preparing
Preeminent Seed. Research Report. Ministry of Research and
Technology. 2009. Unpublished
[3] L.Tort, J.C. Balasch, S. Mackenzie. "Fish immune system. A crossroads
between innate and adaptive responses (Rev.)". Immunología Vol. 22 /
N├║m 3/ 2003, p :277-286
[4] A. Meager. Interleukin. In: Immunology: from cell biology to disease:
Meyers (ed). Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim .
2007, p: 167-204.
[5] S. Hibbeler. Infection-dependent MHC expression in the three-spined
stickleback, Gasterosteus aculeatus (Diplomarbeit). Mathematisch-
Naturwissenschaftlichen Fakultät der Christian-Albrechts-Universität zu
Kiel, Max-Planck-Institut F├╝r Limnologie, Plön, Germany. 2006.
[6] E.O.Koppang, B. H. Dannevig, O. Lie, K. Ronningen and C. McL.
Press. "Expression of Mhc class I and II mRNA in a macrophage-like
cell line (SHK-1) derived from Atlantic salmon, Salmo salar L., head
kidney". Fish & Shellfish Immunology. Vol 9, Issue 6, 1999, pp. 473-
489.
[7] T., Xu, S. Chen, X. Ji and Y. Tian. "MHC polymorphism and disease
resistance to Vibrio anguillarum in 12 selective Japanese flounder
(Paralichthys olivaceus) families". Fish & Shellfish Immunology Vol.
25, Issue 3, 2008, pp. 213-221.
[8] M.G.,Mage, L. Lee, R. K. Ribaudo, M. Corr, S. Kozlowski, L. McHugh,
and D. H. Margulies. "A recombinant, soluble, single-chain class I major
histocompatibility complex molecule with biological activity". Proc.
Natl. Acad. Sci USA. Vol. 89, 1992, pp. 10658-10662.
[9] S.H.E. Kaufman, A. Sher, R. Ahmed. Immunology of Infection Disease.
Washington, DC: ASM Press,. 2002.
[10] B. Dixon, K. E. Magor, B. P. Shum, and P. Parham. Major
Histocompatibility Complex Class I Genes in Rainbow Trout
(Oncorynchus mykiss). Departement of Structural Biology Fairchild
Center, Stanford University School of Medicine, California. 1996.
[11] M.T. Madigan, J.M. Martinko, dan J. Parker. Brock Biology of
Microorganisms, Tenth edition. New Jersey: Prentice Hall, Pearson
education, Inc., 2003, 1019p.
[12] L.F. Hanne, and R.A. Findkelstein, "Characterization and distribution of
the Hemaglutinins produced by Vibrio Cholera". Infect. Immun. Vol 36:
1982, pp. 209-269.
[13] N.K. Nanda, L. Birch, N. M. Greenberg, and G. S. Prins. "MHC Class I
and Class II Molecules Are Expressed in Both Human and Mouse
Prostate Tumor Microenvironment". Prostate ; 66 (12), 2006, pp. 1275-
1284.
[14] E.O.Koppang, M. Lundin, C. McL. Press, K. Ronningen and O. Lie.
Differing levels of Mhc class II β chain expression in a range of tissues
from vaccinated and non-vaccinated Atlantic salmon (Salmo salar.)
Department of Morphology, Genetics and Aquatic Biology, Norwegian
College of Veterinary Medicine, P.O. 8146 .Dep. 0033, Oslo, Norway.
2002.
[15] A. Cuesta, M.A. Esteban, and J. Meseguer. "Cloning, Distribution, and
Up-regulation of the Teleost fish MHC class II alpha as Antigen-
Presenting Cells". Molecular Immunology Vol. 45 Issue 8. March 2006,
pp. 1275-1285.
[16] J.M. Janda. Vibrio, Aeromonas and Plesiomonas. In Balows and
Duerden (Editors), Topley & Wilson- s Microbiology and Microbial
Infections, 9th Ed., Vol. 2, Arnold, London. 1998. pp. 1065-1089.
[17] Kropshofer dan Vogt, 2007 Kropshofer, H. And A. B. Vogt. 2007.
Antigen presenting cells (APCs). In Meyers Immunology from cell
biology to disease (Ed. Meyers, R. A.) wiley VHC-Verlag, 43-91
[18] A.K. Abbas and A. H. Lichtman. Cellular and Molecular Immunology,
fifth edition, updated edition. Pennsylvania:Elsevier saunders, 2005.
[19] G. Drozina, J. Kohoutek, N. J. Ferrat,
and B. M. Peterlin. "Expression
of MHC II Genes". Curr. Top. Microbiol. Immunol 290. 2005.pp147-70.
[1] K. Yuasa, Des Roza, I. Koesharyani, F. Johnny and K. Mahardika.
General Remarks On Fish Disease Diagnosis. Textbook for the Training
Course on Fish Disease Diagnosis. Lolitkanta-JICA Booklet No. 12.
2000, pp. 5-18.
[2] U. Yanuhar. Development of Vaccine based on Receptor Peptide of
Grouper recognizing Antigen as Transgenic Antibody for Preparing
Preeminent Seed. Research Report. Ministry of Research and
Technology. 2009. Unpublished
[3] L.Tort, J.C. Balasch, S. Mackenzie. "Fish immune system. A crossroads
between innate and adaptive responses (Rev.)". Immunología Vol. 22 /
N├║m 3/ 2003, p :277-286
[4] A. Meager. Interleukin. In: Immunology: from cell biology to disease:
Meyers (ed). Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim .
2007, p: 167-204.
[5] S. Hibbeler. Infection-dependent MHC expression in the three-spined
stickleback, Gasterosteus aculeatus (Diplomarbeit). Mathematisch-
Naturwissenschaftlichen Fakultät der Christian-Albrechts-Universität zu
Kiel, Max-Planck-Institut F├╝r Limnologie, Plön, Germany. 2006.
[6] E.O.Koppang, B. H. Dannevig, O. Lie, K. Ronningen and C. McL.
Press. "Expression of Mhc class I and II mRNA in a macrophage-like
cell line (SHK-1) derived from Atlantic salmon, Salmo salar L., head
kidney". Fish & Shellfish Immunology. Vol 9, Issue 6, 1999, pp. 473-
489.
[7] T., Xu, S. Chen, X. Ji and Y. Tian. "MHC polymorphism and disease
resistance to Vibrio anguillarum in 12 selective Japanese flounder
(Paralichthys olivaceus) families". Fish & Shellfish Immunology Vol.
25, Issue 3, 2008, pp. 213-221.
[8] M.G.,Mage, L. Lee, R. K. Ribaudo, M. Corr, S. Kozlowski, L. McHugh,
and D. H. Margulies. "A recombinant, soluble, single-chain class I major
histocompatibility complex molecule with biological activity". Proc.
Natl. Acad. Sci USA. Vol. 89, 1992, pp. 10658-10662.
[9] S.H.E. Kaufman, A. Sher, R. Ahmed. Immunology of Infection Disease.
Washington, DC: ASM Press,. 2002.
[10] B. Dixon, K. E. Magor, B. P. Shum, and P. Parham. Major
Histocompatibility Complex Class I Genes in Rainbow Trout
(Oncorynchus mykiss). Departement of Structural Biology Fairchild
Center, Stanford University School of Medicine, California. 1996.
[11] M.T. Madigan, J.M. Martinko, dan J. Parker. Brock Biology of
Microorganisms, Tenth edition. New Jersey: Prentice Hall, Pearson
education, Inc., 2003, 1019p.
[12] L.F. Hanne, and R.A. Findkelstein, "Characterization and distribution of
the Hemaglutinins produced by Vibrio Cholera". Infect. Immun. Vol 36:
1982, pp. 209-269.
[13] N.K. Nanda, L. Birch, N. M. Greenberg, and G. S. Prins. "MHC Class I
and Class II Molecules Are Expressed in Both Human and Mouse
Prostate Tumor Microenvironment". Prostate ; 66 (12), 2006, pp. 1275-
1284.
[14] E.O.Koppang, M. Lundin, C. McL. Press, K. Ronningen and O. Lie.
Differing levels of Mhc class II β chain expression in a range of tissues
from vaccinated and non-vaccinated Atlantic salmon (Salmo salar.)
Department of Morphology, Genetics and Aquatic Biology, Norwegian
College of Veterinary Medicine, P.O. 8146 .Dep. 0033, Oslo, Norway.
2002.
[15] A. Cuesta, M.A. Esteban, and J. Meseguer. "Cloning, Distribution, and
Up-regulation of the Teleost fish MHC class II alpha as Antigen-
Presenting Cells". Molecular Immunology Vol. 45 Issue 8. March 2006,
pp. 1275-1285.
[16] J.M. Janda. Vibrio, Aeromonas and Plesiomonas. In Balows and
Duerden (Editors), Topley & Wilson- s Microbiology and Microbial
Infections, 9th Ed., Vol. 2, Arnold, London. 1998. pp. 1065-1089.
[17] Kropshofer dan Vogt, 2007 Kropshofer, H. And A. B. Vogt. 2007.
Antigen presenting cells (APCs). In Meyers Immunology from cell
biology to disease (Ed. Meyers, R. A.) wiley VHC-Verlag, 43-91
[18] A.K. Abbas and A. H. Lichtman. Cellular and Molecular Immunology,
fifth edition, updated edition. Pennsylvania:Elsevier saunders, 2005.
[19] G. Drozina, J. Kohoutek, N. J. Ferrat,
and B. M. Peterlin. "Expression
of MHC II Genes". Curr. Top. Microbiol. Immunol 290. 2005.pp147-70.
@article{"International Journal of Biological, Life and Agricultural Sciences:53966", author = "Uun Yanuhar", title = "The Role of Immunogenic Adhesin Vibrio alginolyticus 49 k Da to Molecule Expression of Major Histocompatibility Complex on Receptors of Humpback Grouper Cromileptes altivelis", abstract = "The purpose of research was to know the role of
immunogenic protein of 49 kDa from V.alginolyticus which capable
to initiate molecule expression of MHC Class II in receptor of
Cromileptes altivelis. The method used was in vivo experimental
research through testing of immunogenic protein 49 kDa from
V.alginolyticus at Cromileptes altivelis (size of 250 - 300 grams)
using 3 times booster by injecting an immunogenic protein in a
intramuscular manner. Response of expressed MHC molecule was
shown using immunocytochemistry method and SEM. Results
indicated that adhesin V.alginolyticus 49 kDa which have
immunogenic character could trigger expression of MHC class II on
receptor of grouper and has been proven by staining using
immunocytochemistry and SEM with labeling using antibody anti
MHC (anti mouse). This visible expression based on binding between
epitopes antigen and antibody anti MHC in the receptor. Using
immunocytochemistry, intracellular response of MHC to in vivo
induction of immunogenic adhesin from V.alginolyticus was shown.", keywords = "C.altivelis, immunogenic, MHC, V.alginolyticus.", volume = "4", number = "7", pages = "457-6", }
