Primer Design with Specific PCR Product using Particle Swarm Optimization

Before performing polymerase chain reactions (PCR), a feasible primer set is required. Many primer design methods have been proposed for design a feasible primer set. However, the majority of these methods require a relatively long time to obtain an optimal solution since large quantities of template DNA need to be analyzed. Furthermore, the designed primer sets usually do not provide a specific PCR product. In recent years, evolutionary computation has been applied to PCR primer design and yielded promising results. In this paper, a particle swarm optimization (PSO) algorithm is proposed to solve primer design problems associated with providing a specific product for PCR experiments. A test set of the gene CYP1A1, associated with a heightened lung cancer risk was analyzed and the comparison of accuracy and running time with the genetic algorithm (GA) and memetic algorithm (MA) was performed. A comparison of results indicated that the proposed PSO method for primer design finds optimal or near-optimal primer sets and effective PCR products in a relatively short time.

Seamless Flow of Voluminous Data in High Speed Network without Congestion Using Feedback Mechanism

Continuously growing needs for Internet applications that transmit massive amount of data have led to the emergence of high speed network. Data transfer must take place without any congestion and hence feedback parameters must be transferred from the receiver end to the sender end so as to restrict the sending rate in order to avoid congestion. Even though TCP tries to avoid congestion by restricting the sending rate and window size, it never announces the sender about the capacity of the data to be sent and also it reduces the window size by half at the time of congestion therefore resulting in the decrease of throughput, low utilization of the bandwidth and maximum delay. In this paper, XCP protocol is used and feedback parameters are calculated based on arrival rate, service rate, traffic rate and queue size and hence the receiver informs the sender about the throughput, capacity of the data to be sent and window size adjustment, resulting in no drastic decrease in window size, better increase in sending rate because of which there is a continuous flow of data without congestion. Therefore as a result of this, there is a maximum increase in throughput, high utilization of the bandwidth and minimum delay. The result of the proposed work is presented as a graph based on throughput, delay and window size. Thus in this paper, XCP protocol is well illustrated and the various parameters are thoroughly analyzed and adequately presented.

Fractal Dimension: An Index to Quantify Parameters in Genetic Algorithms

Genetic Algorithms (GAs) are direct searching methods which require little information from design space. This characteristic beside robustness of these algorithms makes them to be very popular in recent decades. On the other hand, while this method is employed, there is no guarantee to achieve optimum results. This obliged designer to run such algorithms more than one time to achieve more reliable results. There are many attempts to modify the algorithms to make them more efficient. In this paper, by application of fractal dimension (particularly, Box Counting Method), the complexity of design space are established for determination of mutation and crossover probabilities (Pm and Pc). This methodology is followed by a numerical example for more clarification. It is concluded that this modification will improve efficiency of GAs and make them to bring about more reliable results especially for design space with higher fractal dimensions.

Distributed Frequency Synchronization for Global Synchronization in Wireless Mesh Networks

In this paper, our focus is to assure a global frequency synchronization in OFDMA-based wireless mesh networks with local information. To acquire the global synchronization in distributed manner, we propose a novel distributed frequency synchronization (DFS) method. DFS is a method that carrier frequencies of distributed nodes converge to a common value by repetitive estimation and averaging step and sharing step. Experimental results show that DFS achieves noteworthy better synchronization success probability than existing schemes in OFDMA-based mesh networks where the estimation error is presented.

Development of Coronal Field and Solar Wind Components for MHD Interplanetary Simulations

The connection between solar activity and adverse phenomena in the Earth’s environment that can affect space and ground based technologies has spurred interest in Space Weather (SW) research. A great effort has been put on the development of suitable models that can provide advanced forecast of SW events. With the progress in computational technology, it is becoming possible to develop operational large scale physics based models which can incorporate the most important physical processes and domains of the Sun-Earth system. In order to enhance our SW prediction capabilities we are developing advanced numerical tools. With operational requirements in mind, our goal is to develop a modular simulation framework of propagation of the disturbances from the Sun through interplanetary space to the Earth. Here, we report and discuss on the development of coronal field and solar wind components for a large scale MHD code. The model for these components is based on a potential field source surface model and an empirical Wang-Sheeley-Arge solar wind relation. 

Identification of Factors Influencing Company's Competitiveness

Fast development of technologies, economic globalization and many other external circumstances stimulate company’s competitiveness. One of the major trends in today’s business is the shift to the exploitation of the Internet and electronic environment for entrepreneurial needs. Latest researches confirm that e-environment provides a range of possibilities and opportunities for companies, especially for micro-, small- and medium-sized companies, which have limited resources. The usage of e-tools raises the effectiveness and the profitability of an organization, as well as its competitiveness. In the electronic market, as in the classic one, there are factors, such as globalization, development of new technology, price sensitive consumers, Internet, new distribution and communication channels that influence entrepreneurship. As a result of eenvironment development, e-commerce and e-marketing grow as well. Objective of the paper: To describe and identify factors influencing company’s competitiveness in e-environment. Research methodology: The authors employ well-established quantitative and qualitative methods of research: grouping, analysis, statistics method, factor analysis in SPSS 20 environment, etc. The theoretical and methodological background of the research is formed by using scientific researches and publications, such as that from mass media and professional literature; statistical information from legal institutions as well as information collected by the authors during the surveying process. Research result: The authors detected and classified factors influencing competitiveness in e-environment.  In this paper, the authors presented their findings based on theoretical, scientific, and field research. Authors have conducted a research on e-environment utilization among Latvian enterprises. 

Application of Lattice Boltzmann Methods in Heat and Moisture Transfer in Frozen Soil

Although water only takes a little percentage in the total mass of soil, it indeed plays an important role to the strength of structure. Moisture transfer can be carried out by many different mechanisms which may involve heat and mass transfer, thermodynamic phase change, and the interplay of various forces such as viscous, buoyancy, and capillary forces. The continuum models are not well suited for describing those phenomena in which the connectivity of the pore space or the fracture network, or that of a fluid phase, plays a major role. However, Lattice Boltzmann methods (LBMs) are especially well suited to simulate flows around complex geometries. Lattice Boltzmann methods were initially invented for solving fluid flows. Recently, fluid with multicomponent and phase change is also included in the equations. By comparing the numerical result with experimental result, the Lattice Boltzmann methods with phase change will be optimized.

Adsorption Kinetics of Alcohols over MCM-41 Materials

Adsorption of methanol and ethanol over mesoporous siliceous material are studied in the current paper. The pure mesoporous silica is prepared using tetraethylorthosilicate (TEOS) as silica source and dodecylamine as template at low pH. The prepared material was characterized using nitrogen adsorption,nX-ray diffraction (XRD) and scanning electron microscopy (SEM). The adsorption kinetics of methanol and ethanol from aqueous solution were studied over the prepared mesoporous silica material. The percent removal of alcohol was calculated per unit mass of adsorbent used. The 1st order model is found to be in agreement with both adsorbates while the 2nd order model fit the adsorption of methanol only.

Universal Kinetic Modeling of RAFT Polymerization using Moment Equations

In the following text, we show that by introducing universal kinetic scheme, the origin of rate retardation and inhibition period which observed in dithiobenzoate-mediated RAFT polymerization can be described properly. We develop our model by utilizing the method of moments, then we apply our model to different monomer/RAFT agent systems, both homo- and copolymerization. The modeling results are in an excellent agreement with experiments and imply the validity of universal kinetic scheme, not only for dithiobenzoate-mediated systems, but also for different types of monomer/RAFT agent ones.

Business Model Topology in Emerging Business Ecosystem

This paper describes topology of business models in market ecosystem of the emerging electric mobility industry. The business model topology shows that firm-s participation in the ecosystem is associated with different requirements on resources and capabilities, and different levels of risk. Business model concept is used together with concepts of networked value creation and shows that firms can achieve higher levels of sustainable advantage by cooperation, not competition. Hybrid business models provide companies a viable alternative possibility for participation in the market ecosystem.

Compact Binary Tree Representation of Logic Function with Enhanced Throughput

An effective approach for realizing the binary tree structure, representing a combinational logic functionality with enhanced throughput, is discussed in this paper. The optimization in maximum operating frequency was achieved through delay minimization, which in turn was possible by means of reducing the depth of the binary network. The proposed synthesis methodology has been validated by experimentation with FPGA as the target technology. Though our proposal is technology independent, yet the heuristic enables better optimization in throughput even after technology mapping for such Boolean functionality; whose reduced CNF form is associated with a lesser literal cost than its reduced DNF form at the Boolean equation level. For cases otherwise, our method converges to similar results as that of [12]. The practical results obtained for a variety of case studies demonstrate an improvement in the maximum throughput rate for Spartan IIE (XC2S50E-7FT256) and Spartan 3 (XC3S50-4PQ144) FPGA logic families by 10.49% and 13.68% respectively. With respect to the LUTs and IOBUFs required for physical implementation of the requisite non-regenerative logic functionality, the proposed method enabled savings to the tune of 44.35% and 44.67% respectively, over the existing efficient method available in literature [12].

Distributed Case Based Reasoning for Intelligent Tutoring System: An Agent Based Student Modeling Paradigm

Online learning with Intelligent Tutoring System (ITS) is becoming very popular where the system models the student-s learning behavior and presents to the student the learning material (content, questions-answers, assignments) accordingly. In today-s distributed computing environment, the tutoring system can take advantage of networking to utilize the model for a student for students from other similar groups. In the present paper we present a methodology where using Case Based Reasoning (CBR), ITS provides student modeling for online learning in a distributed environment with the help of agents. The paper describes the approach, the architecture, and the agent characteristics for such system. This concept can be deployed to develop ITS where the tutor can author and the students can learn locally whereas the ITS can model the students- learning globally in a distributed environment. The advantage of such an approach is that both the learning material (domain knowledge) and student model can be globally distributed thus enhancing the efficiency of ITS with reducing the bandwidth requirement and complexity of the system.

Target Detection with Improved Image Texture Feature Coding Method and Support Vector Machine

An image texture analysis and target recognition approach of using an improved image texture feature coding method (TFCM) and Support Vector Machine (SVM) for target detection is presented. With our proposed target detection framework, targets of interest can be detected accurately. Cascade-Sliding-Window technique was also developed for automated target localization. Application to mammogram showed that over 88% of normal mammograms and 80% of abnormal mammograms can be correctly identified. The approach was also successfully applied to Synthetic Aperture Radar (SAR) and Ground Penetrating Radar (GPR) images for target detection.

Removal of Ciprofloxazin and Carbamazepine by Adsorption on Functionalized Mesoporous Silicates

Ciprofloxacin (CIP) and Carbamazepine (CBZ), nonbiodegradable pharmaceutical residues, were become emerging pollutants in several aquatic environments. The objectives of this research were to study the possibility to recover these pharmaceuticals residues from pharmaceutical wastewater by increasing the selective adsorption on synthesized functionalized porous silicate, comparing with powdered activated carbon (PAC). Hexagonal mesoporous silicate (HMS), functionalized HMSs (3- aminopropyltriethoxy, 3- mercaptopropyltrimethoxy and noctyldimethyl) were synthesized and characterized physico-chemical characteristics. Obtained adsorption kinetics and isotherms showed that 3-mercaptopropyltrimethoxy functional groups grafted on HMS provided highest CIP and CBZ adsorption capacities; however, it was still lower than that of PAC. The kinetic results were compatible with pseudo-second order. The hydrophobicity and hydrogen bonding might play a key role on the adsorption. Furthermore, the capacities were affected by varying pH values due to the strength of hydrogen bonding between targeted compounds and adsorbents. Electrostatic interaction might not affect the adsorption capacities.

Effect of a Magnetic Field on the Onset of Marangoni Convection in a Micropolar Fluid

With the presence of a uniform vertical magnetic field and suspended particles, thermocapillary instability in a horizontal liquid layer is investigated. The resulting eigenvalue is solved by the Galerkin technique for various basic temperature gradients. It is found that the presence of magnetic field always has a stability effect of increasing the critical Marangoni number.

Complex-Valued Neural Network in Image Recognition: A Study on the Effectiveness of Radial Basis Function

A complex valued neural network is a neural network, which consists of complex valued input and/or weights and/or thresholds and/or activation functions. Complex-valued neural networks have been widening the scope of applications not only in electronics and informatics, but also in social systems. One of the most important applications of the complex valued neural network is in image and vision processing. In Neural networks, radial basis functions are often used for interpolation in multidimensional space. A Radial Basis function is a function, which has built into it a distance criterion with respect to a centre. Radial basis functions have often been applied in the area of neural networks where they may be used as a replacement for the sigmoid hidden layer transfer characteristic in multi-layer perceptron. This paper aims to present exhaustive results of using RBF units in a complex-valued neural network model that uses the back-propagation algorithm (called 'Complex-BP') for learning. Our experiments results demonstrate the effectiveness of a Radial basis function in a complex valued neural network in image recognition over a real valued neural network. We have studied and stated various observations like effect of learning rates, ranges of the initial weights randomly selected, error functions used and number of iterations for the convergence of error on a neural network model with RBF units. Some inherent properties of this complex back propagation algorithm are also studied and discussed.

Speech Encryption and Decryption Using Linear Feedback Shift Register (LFSR)

This paper is taken into consideration the problem of cryptanalysis of stream ciphers. There is some attempts need to improve the existing attacks on stream cipher and to make an attempt to distinguish the portions of cipher text obtained by the encryption of plain text in which some parts of the text are random and the rest are non-random. This paper presents a tutorial introduction to symmetric cryptography. The basic information theoretic and computational properties of classic and modern cryptographic systems are presented, followed by an examination of the application of cryptography to the security of VoIP system in computer networks using LFSR algorithm. The implementation program will be developed Java 2. LFSR algorithm is appropriate for the encryption and decryption of online streaming data, e.g. VoIP (voice chatting over IP). This paper is implemented the encryption module of speech signals to cipher text and decryption module of cipher text to speech signals.

Comparative Kinetic Study on Alkylation of p-cresol with Tert-butyl Alcohol using Different SO3-H Functionalized Ionic Liquid Catalysts

Ionic liquids are well known as green solvents, reaction media and catalysis. Here, three different sulfonic acid functional ionic liquids prepared in the laboratory are used as catalysts in alkylation of p-cresol with tert-butyl alcohol. The kinetics on each of the catalysts was compared and a kinetic model was developed based on the product distribution over these catalysts. The kinetic parameters were estimated using Marquadt's algorithm to minimize the error function. The Arrhenius plots show a curvature which is best interpreted by the extended Arrhenius equation.

Robust Design of Power System Stabilizers Using Adaptive Genetic Algorithms

Genetic algorithms (GAs) have been widely used for global optimization problems. The GA performance depends highly on the choice of the search space for each parameter to be optimized. Often, this choice is a problem-based experience. The search space being a set of potential solutions may contain the global optimum and/or other local optimums. A bad choice of this search space results in poor solutions. In this paper, our approach consists in extending the search space boundaries during the GA optimization, only when it is required. This leads to more diversification of GA population by new solutions that were not available with fixed search space boundaries. So, these dynamic search spaces can improve the GA optimization performances. The proposed approach is applied to power system stabilizer optimization for multimachine power system (16-generator and 68-bus). The obtained results are evaluated and compared with those obtained by ordinary GAs. Eigenvalue analysis and nonlinear system simulation results show the effectiveness of the proposed approach to damp out the electromechanical oscillation and enhance the global system stability.

Qualitative Modelling for Ferromagnetic Hysteresis Cycle

In determining the electromagnetic properties of magnetic materials, hysteresis modeling is of high importance. Many models are available to investigate those characteristics but they tend to be complex and difficult to implement. In this paper a new qualitative hysteresis model for ferromagnetic core is presented, based on the function approximation capabilities of adaptive neuro fuzzy inference system (ANFIS). The proposed ANFIS model combined the neural network adaptive capabilities and the fuzzy logic qualitative approach can restored the hysteresis curve with a little RMS error. The model accuracy is good and can be easily adapted to the requirements of the application by extending or reducing the network training set and thus the required amount of measurement data.