Abstract: Tannase (tannin acyl hydrolase, E.C.3.1.1.20) is an
important hydrolysable enzyme with innumerable applications and
industrial potential. In the present study, a kinetic model has been
developed for the batch fermentation used for the production of
tannase by A.flavus MTCC 3783. Maximum tannase activity of
143.30 U/ml was obtained at 96 hours under optimum operating
conditions at 35oC, an initial pH of 5.5 and with an inducer tannic
acid concentration of 3% (w/v) for a fermentation period of 120
hours. The biomass concentration reaches a maximum of 6.62 g/l at
96 hours and further there was no increase in biomass concentration
till the end of the fermentation. Various unstructured kinetic models
were analyzed to simulate the experimental values of microbial
growth, tannase activity and substrate concentration. The Logistic
model for microbial growth , Luedeking - Piret model for production
of tannase and Substrate utilization kinetic model for utilization of
substrate were capable of predicting the fermentation profile with
high coefficient of determination (R2) values of 0.980, 0.942 and
0.983 respectively. The results indicated that the unstructured models
were able to describe the fermentation kinetics more effectively.
Abstract: A novel chromium-free protective coating films based
on a zeolite coating was growing onto a FeCrAlloy metal using in –
situ hydrothermal method. The zeolite film was obtained using in-situ
crystallization process that is capable of coating large surfaces with
complex shape and in confined spaces has been developed. The
zeolite coating offers an advantage of a high mechanical stability and
thermal stability. The physicochemical properties were investigated
using X-ray diffraction (XRD), Electron Microscopy (SEM), Energy
Dispersive X–ray Analysis (EDX) and Thermogravimetric Analysis
(TGA). The transition from oxide-on-alloy wires to hydrothermally
synthesised uniformly zeolite coated surfaces was followed using
SEM and XRD. In addition, the robustness of the prepared coating
was confirmed by subjecting these to thermal cycling (ambient to
550oC).
Abstract: The use of engineered nanomaterials has increased as
a result of their positive impact on many sectors of the economy,
including agriculture. Silver nanoparticles (AgNPs) are now used to
enhance seed germination, plant growth, and photosynthetic quantum
efficiency and as antimicrobial agents to control plant diseases. In
this study, we examined the effect of AgNP dosage on the seed
germination of three plant species: corn (Zea mays L.), watermelon
(Citrullus lanatus [Thunb.] Matsum. & Nakai) and zucchini
(Cucurbita pepo L.). This experiment was designed to study the
effect of AgNPs on germination percentage, germination rate, mean
germination time, root length and fresh and dry weight of seedlings
for the three species. Seven concentrations (0.05, 0.1, 0.5, 1, 1.5, 2
and 2.5 mg/ml) of AgNPs were examined at the seed germination
stage. The three species had different dose responses to AgNPs in
terms of germination parameters and the measured growth
characteristics. The germination rates of the three plants were
enhanced in response to AgNPs. Significant enhancement of the
germination percentage values was observed after treatment of the
watermelon and zucchini plants with AgNPs in comparison with
untreated seeds. AgNPs showed a toxic effect on corn root
elongation, whereas watermelon and zucchini seedling growth were
positively affected by certain concentrations of AgNPs. This study
showed that exposure to AgNPs caused both positive and negative
effects on plant growth and germination.
Abstract: Several embryonic cellular mechanism including cell
cycle, growth and apoptosis are regulated by phosphatidylinositol-3-
kinase (PI3K)/Akt signaling pathway. The goal of present study is to
determine the effects of annatto (Bixa orellana)-derived δ-tocotrienol
(δ-TCT) on the regulations of PI3K/Akt genes in murine morula.
Twenty four 6-8 week old (23-25g) female balb/c mice were
randomly divided into four groups (G1-G4; n=6). Those groups were
subjected to the following treatments for 7 consecutive days: G1
(control) received tocopherol stripped corn oil, G2 was given 60
mg/kg/day of δ-TCT mixture (contains 90% delta & 10% gamma
isomers), G3 was given 60 mg/kg/day of pure δ-TCT (>98% purity)
and G4 received 60 mg/kg/day α-TOC. On Day 8, females were
superovulated with 5 IU Pregnant Mare’s Serum Gonadotropin
(PMSG) for 48 hours followed with 5 IU human Chorionic
Gonadotropin (hCG) before mated with males at the ratio of 1:1.
Females were sacrificed by cervical dislocation for embryo collection
48 hours post-coitum. About fifty morulas from each group were
used in the gene expression analyses using Affymetrix QuantiGene
Plex 2.0 Assay. Present data showed a significant increase (p
Abstract: Human beings have the ability to make logical
decisions. Although human decision - making is often optimal, it is
insufficient when huge amount of data is to be classified. Medical
dataset is a vital ingredient used in predicting patient’s health
condition. In other to have the best prediction, there calls for most
suitable machine learning algorithms. This work compared the
performance of Artificial Neural Network (ANN) and Decision Tree
Algorithms (DTA) as regards to some performance metrics using
diabetes data. WEKA software was used for the implementation of
the algorithms. Multilayer Perceptron (MLP) and Radial Basis
Function (RBF) were the two algorithms used for ANN, while
RegTree and LADTree algorithms were the DTA models used. From
the results obtained, DTA performed better than ANN. The Root
Mean Squared Error (RMSE) of MLP is 0.3913 that of RBF is
0.3625, that of RepTree is 0.3174 and that of LADTree is 0.3206
respectively.
Abstract: DNA Barcode provides good sources of needed
information to classify living species. The classification problem has
to be supported with reliable methods and algorithms. To analyze
species regions or entire genomes, it becomes necessary to use the
similarity sequence methods. A large set of sequences can be
simultaneously compared using Multiple Sequence Alignment which
is known to be NP-complete. However, all the used methods are still
computationally very expensive and require significant computational
infrastructure. Our goal is to build predictive models that are highly
accurate and interpretable. In fact, our method permits to avoid the
complex problem of form and structure in different classes of
organisms. The empirical data and their classification performances
are compared with other methods. Evenly, in this study, we present
our system which is consisted of three phases. The first one, is called
transformation, is composed of three sub steps; Electron-Ion
Interaction Pseudopotential (EIIP) for the codification of DNA
Barcodes, Fourier Transform and Power Spectrum Signal Processing.
Moreover, the second phase step is an approximation; it is
empowered by the use of Multi Library Wavelet Neural Networks
(MLWNN). Finally, the third one, is called the classification of DNA
Barcodes, is realized by applying the algorithm of hierarchical
classification.
Abstract: The lifetime of a wireless sensor network can be
effectively increased by using scheduling operations. Once the
sensors are randomly deployed, the task at hand is to find the largest
number of disjoint sets of sensors such that every sensor set provides
complete coverage of the target area. At any instant, only one of these
disjoint sets is switched on, while all other are switched off. This
paper proposes a heuristic search method to find the maximum
number of disjoint sets that completely cover the region. A
population of randomly initialized members is made to explore the
solution space. A set of heuristics has been applied to guide the
members to a possible solution in their neighborhood. The heuristics
escalate the convergence of the algorithm. The best solution explored
by the population is recorded and is continuously updated. The
proposed algorithm has been tested for applications which require
sensing of multiple target points, referred to as point coverage
applications. Results show that the proposed algorithm outclasses the
existing algorithms. It always finds the optimum solution, and that
too by making fewer number of fitness function evaluations than the
existing approaches.
Abstract: These Monolayer and multilayer coatings of CrN and
AlCrN deposited on 100Cr6 (AISI 52100) substrate by PVD
magnetron sputtering system. The microstructures of the coatings
were characterized using atomic force microscopy (AFM). The AFM
analysis revealed the presence of domes and craters that are
uniformly distributed over all surfaces of the various layers.
Nanoindentation measurement of CrN coating showed maximum
hardness (H) and modulus (E) of 14 GPa and 190 GPa, respectively.
The measured H and E values of AlCrN coatings were found to be 30
GPa and 382 GPa, respectively. The improved hardness in both the
coatings was attributed mainly to a reduction in crystallite size and
decrease in surface roughness. The incorporation of Al into the CrN
coatings has improved both hardness and Young’s modulus.
Abstract: We apply the non-parametric, unconditional,
hyperbolic order-α quantile estimator to appraise the relative
efficiency of Microfinance Institutions in Africa in terms of outreach.
Our purpose is to verify if these institutions, which must constantly
try to strike a compromise between their social role and financial
sustainability are operationally efficient.
Using data on African MFIs extracted from the Microfinance
Information eXchange (MIX) database and covering the 2004 to
2006 periods, we find that more efficient MFIs are also the most
profitable. This result is in line with the view that social performance
is not in contradiction with the pursuit of excellent financial
performance. Our results also show that large MFIs in terms of asset
and those charging the highest fees are not necessarily the most
efficient.
Abstract: This paper investigates the parametric stability of an
axially moving web subjected to non-uniform in-plane edge
excitations on two opposite, simply-supported edges. The web is
modeled as a viscoelastic plate whose constitutive relation obeys the
Kelvin-Voigt model, and the in-plane edge excitations are expressed
as the sum of a static tension and a periodical perturbation. Due to the
in-plane edge excitations, the moving plate may bring about
parametric instability under certain situations. First, the in-plane
stresses of the plate due to the non-uniform edge excitations are
determined by solving the in-plane forced vibration problem. Then,
the dependence on the spatial coordinates in the equation of transverse
motion is eliminated by the generalized Galerkin method, which
results in a set of discretized system equations in time. Finally, the
method of multiple scales is utilized to solve the set of system
equations analytically if the periodical perturbation of the in-plane
edge excitations is much smaller as compared with the static tension of
the plate, from which the stability boundaries of the moving plate are
obtained. Numerical results reveal that only combination resonances
of the summed-type appear under the in-plane edge excitations
considered in this work.
Abstract: The article describes the effect of the replacement of
the used reference coordinate system in the georeferencing of an old
map of Europe. The map was georeferenced into three types of
projection – the equal-area conic (original cartographic projection),
cylindrical Plate Carrée and cylindrical Mercator map projection. The
map was georeferenced by means of the affine and the second-order
polynomial transformation. The resulting georeferenced raster
datasets from the Plate Carrée and Mercator projection were
projected into the equal-area conic projection by means of projection
equations. The output is the comparison of drawn graphics, the
magnitude of standard deviations for individual projections and types
of transformation.
Abstract: Sodium formate is the chemical substance used for
food additive. Catalase is the important antioxidative enzyme in
protecting the cell from oxidative damage by reactive oxygen species
(ROS). The resultant level of oxidative stress in sodium formatetreated
lymphocytes was investigated. The sodium formate
concentrations of 0.05, 0.1, 0.2, 0.4 and 0.6 mg/mL were treated in
human lymphocytes for 12 hours. After 12 treated hours, catalase
activity change was measured in sodium formate-treated
lymphocytes. The results showed that the sodium formate
concentrations of 0.4 and 0.6 mg/mL significantly decreased catalase
activities in lymphocytes (P < 0.05). The change of catalase activity
in sodium formate-treated lymphocytes may be the oxidative damage
marker for detect sodium formate exposure in human.
Abstract: In this study, lipid-deprived residuals of microalgae
were hydrolyzed for the production of reducing sugars by using the
recombinant Bacillus cellulosome, carrying eight genes from the
Clostridium thermocellum ATCC27405. The obtained cellulosome
was found to exist mostly in the broth supernatant with a cellulosome
activity of 2.4 U/mL. Furthermore, the Michaelis-Menten constant
(Km) and Vmax of cellulosome were found to be 14.832 g/L and 3.522
U/mL. The activation energy of the cellulosome to hydrolyze
microalgae LDRs was calculated as 32.804 kJ/mol.
Abstract: SARA is a common and serious metabolic disorder in
early lactation in dairy cattle and in finishing beef cattle, caused by
diets with high inclusion of cereal grain. This experiment was
performed to determine the efficacy of Megasphaera elsdenii, a
major lactate-utilizing bacterium in prevention/treatment of SARA in
vivo. In vivo experimentation, it was used eight ruminally cannulated
rams and it was applied the rapid adaptation with the mixture of grain
based on wheat (80% wheat, 20% barley) and barley (80% barley,
20% wheat). During the systematic adaptation, it was followed the
probability of SARA formation by being measured the rumen pH
with two hours intervals after and before feeding. After being
evaluated the data, it was determined the ruminal pH ranged from
5.2-5.6 on the condition of feeding with 60 percentage of grain
mixture based on barley and wheat, that assured the definite form of
subacute acidosis. In four days SARA period, M. elsdenii (1010 cfu
ml-1) was inoculated during the first two days. During the SARA
period, it was observed the decrease of feed intake with M. elsdenii
inoculation. Inoculation of M. elsdenii was caused to differentiation
of rumen pH (P
Abstract: This paper presents the design and fabrication of a
novel piezoelectric actuator for a gas micro pump with check valve
having the advantages of miniature size, light weight and low power
consumption. The micro pump is designed to have eight major
components, namely a stainless steel upper cover layer, a piezoelectric
actuator, a stainless steel diaphragm, a PDMS chamber layer, two
stainless steel channel layers with two valve seats, a PDMS check
valve layer with two cantilever-type check valves and an acrylic
substrate. A prototype of the gas micro pump, with a size of 52 mm ×
50 mm × 5.0 mm, is fabricated by precise manufacturing. This device
is designed to pump gases with the capability of performing the
self-priming and bubble-tolerant work mode by maximizing the stroke
volume of the membrane as well as the compression ratio via
minimization of the dead volume of the micro pump chamber and
channel. By experiment apparatus setup, we can get the real-time
values of the flow rate of micro pump and the displacement of the
piezoelectric actuator, simultaneously. The gas micro pump obtained
higher output performance under the sinusoidal waveform of 250 Vpp.
The micro pump achieved the maximum pumping rates of 1185
ml/min and back pressure of 7.14 kPa at the corresponding frequency
of 120 and 50 Hz.
Abstract: Breast cancer is in the top rate of cancer. We analyzed
the prevalence of obesity and its association with breast cancer and
finally we reviewed 25 article that 320 patient and 320 control which
enrolled to our study. The distribution of breast cancer patients and
controls with respect to their anthropometric indices in patients with
higher weight, which was statistically significant (60.2 ± 10.2 kg)
compared with control group (56.1 ± 11.3 kg). The body mass index
of patients was (26.06+/-3.42) and significantly higher than the
control group (24.1+/-1.7). Obesity leads to increased levels of
adipose tissue in the body that can be stored toxins and carcinogens
to produce a continuous supply. Due to the high level of fat and the
role of estrogen in a woman which is endogenous estrogen of the
tumor and regulates the activities of growth steroids, obesity has
confirmed as a risk factor for breast cancer. Our study and other
studies have shown that obesity is a risk factor for breast cancer. And
it can be prevented with a weight loss intervention for breast cancer
in the future.
Abstract: Waste Load Allocation (WLA) strategies usually
intend to find economic policies for water resource management.
Water quality trading (WQT) is an approach that uses discharge
permit market to reduce total environmental protection costs. This
primarily requires assigning discharge limits known as total
maximum daily loads (TMDLs). These are determined by monitoring
organizations with respect to the receiving water quality and
remediation capabilities. The purpose of this study is to compare two
approaches of TMDL assignment for WQT policy in small catchment
area of Haraz River, in north of Iran. At first, TMDLs are assigned
uniformly for the whole point sources to keep the concentrations of
BOD and dissolved oxygen (DO) at the standard level at checkpoint
(terminus point). This was simply simulated and controlled by
Qual2kw software. In the second scenario, TMDLs are assigned
using multi objective particle swarm optimization (MOPSO) method
in which the environmental violation at river basin and total treatment
costs are minimized simultaneously. In both scenarios, the equity
index and the WLA based on trading discharge permits (TDP) are
calculated. The comparative results showed that using economically
optimized TMDLs (2nd scenario) has slightly more cost savings rather
than uniform TMDL approach (1st scenario). The former annually
costs about 1 M$ while the latter is 1.15 M$. WQT can decrease
these annual costs to 0.9 and 1.1 M$, respectively. In other word,
these approaches may save 35 and 45% economically in comparison
with command and control policy. It means that using multi objective
decision support systems (DSS) may find more economical WLA,
however its outcome is not necessarily significant in comparison with
uniform TMDLs. This may be due to the similar impact factors of
dischargers in small catchments. Conversely, using uniform TMDLs
for WQT brings more equity that makes stakeholders not feel that
much envious of difference between TMDL and WQT allocation. In
addition, for this case, determination of TMDLs uniformly would be
much easier for monitoring. Consequently, uniform TMDL for TDP
market is recommended as a sustainable approach. However,
economical TMDLs can be used for larger watersheds.
Abstract: Background: Delayed onset muscle soreness (DOMS)
is the most common symptom when ordinary individuals and athletes
are exposed to unaccustomed physical activity, especially eccentric
contraction which impairs athletic performance, ordinary people
work ability and physical functioning. Multitudes of methods have
been investigated to reduce DOMS. One of the valuable methods to
control DOMS is repeated bout effect (RBE) as a prophylactic
method. Purpose: To compare the repeated bout effect of
submaximal eccentric with maximal isometric contraction on induced
DOMS. Methods: Sixty normal male volunteers were assigned
randomly into three equal groups: Group A (first study group): 20
subjects received submaximal eccentric contraction on non-dominant
elbow flexors as a prophylactic exercise. Group B (second study
group): 20 subjects received maximal isometric contraction on nondominant
elbow flexors as a prophylactic exercise. Group C (control
group): 20 subjects did not receive any prophylactic exercises.
Maximal isometric peak torque of elbow flexors and patient related
elbow evaluation (PREE) scale were measured for each subject 3
times before, immediately after, and 48 hours after induction of
DOMS. Results: Post-hoc test for maximal isometric peak torque and
PREE scale immediately and 48 hours after induction of DOMS
revealed that group (A) and group (B) resulted in significant decrease
in maximal isometric strength loss and elbow pain and disability
rather than control group (C), but submaximal eccentric group (A)
was more effective than maximal isometric group (B) as it showed
more rapid recovery of functional strength and less degrees of elbow
pain and disability. Conclusion: Both submaximal eccentric
contraction and maximal isometric contraction were effective in
prevention of DOMS but submaximal eccentric contraction produced
a greater protective effect against muscle damage induced by
maximal eccentric exercise performed 2 days later.
Abstract: One of the functions of the commercial heavy vehicle
is to safely and efficiently transport goods and people. Due to its size
and carrying capacity, it is important to study the vehicle dynamic
stability during cornering. Study has shown that there are a number of
overloaded heavy vehicles or permissible Gross Vehicle Weight
(GVW) violations recorded at selected areas in Malaysia assigned by
its type and category. Thus, the objective of this study is to
investigate the correlation and effect of the GVW on heavy vehicle
stability during cornering event using simulation. Various selected
heavy vehicle types and category are simulated using IPG/Truck
Maker® with different GVW and road condition (coefficient of
friction of road surface), while the speed, driver characteristic, center
of gravity of load and road geometry are constant. Based on the
analysis, the relationship between GVW and lateral acceleration were
established. As expected, on the same value of coefficient of friction,
the maximum lateral acceleration would be increased as the GVW
increases.
Abstract: It is an established fact that polymers have several
physical limitations such as low stiffness and low resistance to
impact on loading. Hence, polymers do not usually have requisite
mechanical strength for application in various fields. The
reinforcement by high strength fibers provides the polymer
substantially enhanced mechanical properties and makes them more
suitable for a large number of diverse applications. This research
evaluates the effects of particulate Cow bone and Groundnut shell
additions on the mechanical properties and microstructure of cow
bone and groundnut shell reinforced epoxy composite in order to
assess the possibility of using it as a material for engineering
applications. Cow bone and groundnut shell particles reinforced with
epoxy (CBRPC and GSRPC) was prepared by varying the cow bone
and groundnut shell particles from 0-25 wt% with 5 wt% intervals. A
Hybrid of the Cow bone and Groundnut shell (HGSCB) reinforce
with epoxy was also prepared. The mechanical properties of the
developed composites were investigated. Optical microscopy was
used to examine the microstructure of the composites. The results
revealed that mechanical properties did not increase uniformly with
additions in filler but exhibited maximum properties at specific
percentages of filler additions. From the Microscopic evaluation, it
was discovered that homogeneity decreases with increase in % filler,
this could be due to poor interfacial bonding.