Abstract: The paper outlines the relevance of computational
geometry within the design and production process of architecture.
Based on two case studies, the digital chain - from the initial formfinding
to the final realization of spatial concepts - is discussed in
relation to geometric principles. The association with the fascinating
complexity that can be found in nature and its underlying geometry
was the starting point for both projects presented in the paper. The
translation of abstract geometric principles into a three-dimensional
digital design model – realized in Rhinoceros – was followed by a
process of transformation and optimization of the initial shape that
integrated aesthetic, spatial and structural qualities as well as aspects
of material properties and conditions of production.
Abstract: A simple and dexterous in situ method was introduced to load CdS nanocrystals into organofunctionalized mesoporous, which used an ion-exchange method. The products were extensively characterized by combined spectroscopic methods. X- ray diffraction (XRD) and high-resolution transmission electron microscopy (HRTEM) demonstrated both the maintenance of pore symmetry (space group p6mm) of SBA-15 and the presence of CdS nanocrystals with uniform sizes of about 6 - 8 nm inside the functionalized SBA-15 channels. These mesoporous silica-supported CdS composites showed room temperature photoluminescence properties with a blue shift, indicating the quantum size effect of nanocrystalline CdS.
Abstract: The aim of this study is evaluating the antinociceptive
and anti-inflamatory activity of Geum kokanicum. After
determination total extract LD50, different doses of extract were
chosen for intrapritoneal injections. In inflammation test, male NMRI
mice were divided into 6 groups: control (normal saline), positive
control (Dexamethasone 15mg/kg), and total extract (0.025, 0.05,
0.1, and 0.2 gr/kg). The inflammation was produced by xyleneinduced
edema. In order to evaluate the antinociceptive effect of total
extract, formalin test was used. Mice were divided into 6 groups:
control, positive control (morphine 10mg/kg), and 4 groups which
received total extract. Then they received Formalin. The animals
were observed for the reaction to pain. Data were analyzed using
One-way ANOVA followed by Tukey-Kramer multiple comparison
test. LD50 was 1 gr/kg. Data indicated that 0.5,0.1 and 0.2 gr/kg
doses of total extract have particular antinociceptive and antiinflammatory
effects in a comparison with control (P
Abstract: Ethanol is generally used as a therapeutic reagent against Hepatocellular carcinoma (HCC or hepatoma) worldwide, as it can induce Hepatocellular carcinoma cell apoptosis at low concentration through a multifactorial process regulated by several unknown proteins. This paper provides a simple and available proteomic strategy for exploring differentially expressed proteins in the apoptotic pathway. The appropriate concentrations of ethanol required to induce HepG2 cell apoptosis were first assessed by MTT assay, Gisma and fluorescence staining. Next, the central proteins involved in the apoptosis pathway processs were determined using 2D-PAGE, SDS-PAGE, and bio-software analysis. Finally the downregulation of two proteins, AFP and survivin, were determined by immunocytochemistry and reverse transcriptase PCR (RT-PCR) technology. The simple, useful method demonstrated here provides a new approach to proteomic analysis in key bio-regulating process including proliferation, differentiation, apoptosis, immunity and metastasis.
Abstract: Identifying the nature of protein-nanoparticle
interactions and favored binding sites is an important issue in
functional characterization of biomolecules and their physiological
responses. Herein, interaction of silver nanoparticles with lysozyme
as a model protein has been monitored via fluorescence spectroscopy.
Formation of complex between the biomolecule and silver
nanoparticles (AgNPs) induced a steady state reduction in the
fluorescence intensity of protein at different concentrations of
nanoparticles. Tryptophan fluorescence quenching spectra suggested
that silver nanoparticles act as a foreign quencher, approaching the
protein via this residue. Analysis of the Stern-Volmer plot showed
quenching constant of 3.73 μM−1. Moreover, a single binding site in
lysozyme is suggested to play role during interaction with AgNPs,
having low affinity of binding compared to gold nanoparticles.
Unfolding studies of lysozyme showed that complex of lysozyme-
AgNPs has not undergone structural perturbations compared to the
bare protein. Results of this effort will pave the way for utilization of
sensitive spectroscopic techniques for rational design of
nanobiomaterials in biomedical applications.
Abstract: Chlorine is one of the most abundant elements in
nature, which undergoes a complex biogeochemical cycle. Chlorine
bound in some substances is partly responsible for atmospheric ozone
depletion and contamination of some ecosystems. As due to
international regulations anthropogenic burden of volatile
organochlorines (VOCls) in atmosphere decreases, natural sources
(plants, soil, abiotic formation) are expected to dominate VOCl
production in the near future. Examples of plant VOCl production are
methyl chloride, and bromide emission from (sub)tropical ferns,
chloroform, 1,1,1-trichloroethane and tetrachloromethane emission
from temperate forest fern and moss. Temperate forests are found to
emit in addition to the previous compounds tetrachloroethene, and
brominated volatile compounds. VOCls can be taken up and further
metabolized in plants. The aim of this work is to identify and
quantitatively analyze the formed VOCls in temperate forest
ecosystems by a cryofocusing/GC-ECD detection method, hence
filling a gap of knowledge in the biogeochemical cycle of chlorine.
Abstract: Champs Bourcin black grape originated from
Aquitaine, France and planted in Sapa, Lao cai provice, exhibited
high total acidity (11.72 g/L). After 9 days of alcoholic fermentation
at 25oC using Saccharomyces cerevisiae UP3OY5 strain, the ethanol
concentration of wine was 11.5% v/v, however the sharp sour taste of
wine has been found. The malolactic fermentation (MLF) was carried
out by Oenococcus oeni ATCCBAA-1163 strain which had been preadapted
to acid (pH 3-4) and ethanol (8-12%v/v) conditions. We
obtained the highest vivability (83.2%) upon malolactic fermentation
after 5 days at 22oC with early stationary phase O. oeni cells preadapted
to pH 3.5 and 8% v/v ethanol in MRS medium. The malic
acid content in wine was decreased from 5.82 g/L to 0.02 g/L after
MLF (21 days at 22oC). The sensory quality of wine was
significantly improved.
Abstract: Every day human life experiences new equipments
more automatic and with more abilities. So the need for faster
processors doesn-t seem to finish. Despite new architectures and
higher frequencies, a single processor is not adequate for many
applications. Parallel processing and networks are previous solutions
for this problem. The new solution to put a network of resources on a
chip is called NOC (network on a chip). The more usual topology for
NOC is mesh topology. There are several routing algorithms suitable
for this topology such as XY, fully adaptive, etc. In this paper we
have suggested a new algorithm named Intermittent X, Y (IX/Y). We
have developed the new algorithm in simulation environment to
compare delay and power consumption with elders' algorithms.
Abstract: We present a system that finds road boundaries and
constructs the virtual lane based on fusion data from a laser and a
monocular sensor, and detects forward vehicle position even in no lane
markers or bad environmental conditions. When the road environment
is dark or a lot of vehicles are parked on the both sides of the road, it is
difficult to detect lane and road boundary. For this reason we use
fusion of laser and vision sensor to extract road boundary to acquire
three dimensional data. We use parabolic road model to calculate road
boundaries which is based on vehicle and sensors state parameters and
construct virtual lane. And then we distinguish vehicle position in each
lane.
Abstract: Inorganic nanoparticles filled polymer composites
have extended their multiple functionalities to various applications,
including mechanical reinforcement, gas barrier, dimensional
stability, heat distortion temperature, flame-retardant, and thermal
conductivity. Sodium stearate-modified calcium carbonate (CaCO3)
nanoparticles were prepared using surface modification method. The
results showed that sodium stearate attached to the surface of CaCO3
nanoparticles with the chemical bond. The effect of modified CaCO3
nanoparticles on thermal properties of polypropylene (PP) was
studied by means of differential scanning calorimetry (DSC) and
Thermogravimetric analysis (TGA). It was found that CaCO3
significantly affected the crystallization temperature and
crystallization degree of PP. Effect of the modified CaCO3 content on
mechanical properties of PP/CaCO3 nanocomposites was also
studied. The results showed that the modified CaCO3 can effectively
improve the mechanical properties of PP. In comparison with PP, the
impact strength of PP/CaCO3 nanocomposites increased by about
65% and the hardness increased by about 5%.
Abstract: In present study the effects of anti-inflammatory and
antinociceptive of vitex hydro-alcoholic extract were evaluated on
male mice. In inflammatory test mice were divided into 7 groups:
first group was control. The second group, positive control group,
received dexamethasone (15 mg/kg) and the other five groups
received different doses of hydroalcohol extract of Vitex fruit (265,
365, 465, 565, and 665 mg/kg). The inflammation was caused by
xylene-induced ear edema. Formalin test was used for evaluation of
antinociceptive effect of extract. In this test, mice were divided into 7
groups: control, morphine (10mg/kg) as positive control group, and
Vitex extract groups ((265, 365, 465, 565, and 665 mg/kg). All drugs
were administered intrapritoneally, 30 min before each test. The data
were analyzed using one-way ANOVA followed by Tukey-kramer
multiple comparison test. Results have shown significant antiinflammatory
effects of extract at all dosed as compared with control
(P
Abstract: Analysis for the propagation of elastic waves in
arbitrary anisotropic plates is investigated, commencing with a
formal analysis of waves in a layered plate of an arbitrary anisotropic
media, the dispersion relations of elastic waves are obtained by
invoking continuity at the interface and boundary of conditions on
the surfaces of layered plate. The obtained solutions can be used for
material systems of higher symmetry such as monoclinic,
orthotropic, transversely isotropic, cubic, and isotropic as it is
contained implicitly in the analysis. The cases of free layered plate
and layered half space are considered separately. Some special cases
have also been deduced and discussed. Finally numerical solution of
the frequency equations for an aluminum epoxy is carried out, and
the dispersion curves for the few lower modes are presented. The
results obtained theoretically have been verified numerically and
illustrated graphically.
Abstract: Empty Fruit Bunches (EFB) and Palm Oil Mill
Effluent (POME) are two main wastes from oil palm industries which
contain rich lignocellulose. Degradation of EFB and POME by
microorganisms will produce hydrolytic enzyme which will degrade
cellulose and hemicellulose during composting process. However,
normal composting takes about four to six months to reach maturity.
Hence, application of fungi into compost can shorten the period of
composting. This study identifies the effect of xylanase and cellulase
produced by Aspergillus niger and Trichoderma virens on
composting process using EFB and POME. The degradation of EFB
and POME indicates the lignocellulolytic capacity of Aspergillus
niger and Trichoderma virens with more than 7% decrease in
hemicellulose and more than 25% decrease in cellulose for both
inoculated compost. Inoculation of Aspergillus niger and
Trichoderma virens also increased the enzyme activities during the
composting period compared to the control compost by 21% for both
xylanase and cellulase. Rapid rise in the activities of cellulase and
xylanase was observed by Aspergillus niger with the highest
activities of 14.41 FPU/mg and 3.89 IU/mg, respectively. Increased
activities of cellulase and xylanase also occurred in inoculation of
Trichoderma virens with the highest activities obtained at 13.21
FPU/mg and 4.43 IU/mg, respectively. Therefore, it is evident that
the inoculation of fungi can increase the enzyme activities hence
effectively degrading the EFB and POME.
Abstract: Pretreatment of lignocellulosic biomass materials from
poplar, acacia, oak, and fir with different ionic liquids (ILs)
containing 1-alkyl-3-methyl-imidazolium cations and various anions
has been carried out. The dissolved cellulose from biomass was
precipitated by adding anti-solvents into the solution and vigorous
stirring. Commercial cellulases Celluclast 1.5L and Accelerase 1000
have been used for hydrolysis of untreated and pretreated
lignocellulosic biomass. Among the tested ILs, [Emim]COOCH3
showed the best efficiency, resulting in highest amount of liberated
reducing sugars. Pretreatment of lignocellulosic biomass using
glycerol-ionic liquids combined pretreatment and dilute acid-ionic
liquids combined pretreatment were evaluated and compared with
glycerol pretreatment, ionic liquids pretreatment and dilute acid
pretreatment.
Abstract: Fip-gts, an immunomodulatory protein purified from Ganoderma tsugae, has been reported to possess therapeutic effects in the treatment of cancer and autoimmune disease. For medicinal application, a recombinant Fip-gts was successfully expressed and purified in Sf21 insect cells by our previously work. It is important to evaluate the immunomodulatory activity of the rFip-gts. To assess the immunomodulatory potential of rFip-gts, the T lymphocytes of murine splenocytes were used in the present study. Results revealed that rFip-gts induced cellular aggregation formation. Additionally, the expression of IL-2 and IFN-r were up-regulated after the treatment of rFip-gts, and a corresponding increased production of IL-2 and IFN-r in a dose-dependent manner. The results showed that rFip-gts has an immunomodulatory activity in inducing Th1 lymphocytes from murine splenocytes released IL-2 and IFN-γ, thus suggest that rFip-gts may have therapeutic potential in vivo as an immune modulator.
Abstract: This paper present some preliminary work on the
preparation and physicochemical caracterization of nanocomposite
MFI-alumina structures based on alumina hollow fibres. The fibers
are manufactured by a wet spinning process. α-alumina particles were
dispersed in a solution of polysulfone in NMP. The resulting slurry is
pressed through the annular gap of a spinneret into a precipitation
bath. The resulting green fibres are sintered. The mechanical strength
of the alumina hollow fibres is determined by a three-point-bending
test while the pore size is characterized by bubble-point testing. The
bending strength is in the range of 110 MPa while the average pore
size is 450 nm for an internal diameter of 1 mm and external diameter
of 1.7 mm. To characterize the MFI membranes various techniques
were used for physicochemical characterization of MFI–ceramic
hollow fibres membranes: The nitrogen adsorption, X-ray
diffractometry, scanning electron microscopy combined with X
emission microanalysis. Scanning Electron Microscopy (SEM) and
Energy Dispersive Microanalysis by the X-ray were used to observe
the morphology of the hollow fibre membranes (thickness,
infiltration into the carrier, defects, homogeneity). No surface film,
has been obtained, as observed by SEM and EDX analysis and
confirmed by high temperature variation of N2 and CO2 gas
permeances before cation exchange. Local analysis and characterise
(SEM and EDX) and overall (by ICP elemental analysis) were
conducted on two samples exchanged to determine the quantity and
distribution of the cation of cesium on the cross section fibre of the
zeolite between the cavities.
Abstract: Repeated additions of the unfertilized bacteria led to
increase the activity of Nitrogen-fixing bacteria in the root zone with
drip irrigation system compared to traditional manual vaccination to
increase the proportion of Nitrogen from 29% to 64%, and the
efficiency of adding Nitrogen fertilizer did not exceed 9.5% while
dropped to 4%, due to the amount of fertilizer added was not exceed
20kg N/h, and the second was the existence of a large amount of
available Nitrogen in the soil by fixation, while the efficiency of
irrigation system between 2.08 to 2.26 kg/m3.
Abstract: The fluorescent pseudomonad strain R81 is a root
colonizing rhizobacteria which promotes the growth of many plants
by various mechanisms. Its broth containing siderophore (ironchelating
compound) and 2,4- diacetyl phloroglucinol (DAPG) is
used for preparing bioinoculant formulations for agronomical
applications. Glycerol was found to be the best carbon source for
improved biomass production. Splitting of nitrogen source to NH4Cl
and urea had a stabilizing effect on pH during batch cultivation. Ltryptophan
at 0.5 % in the medium increased the siderophore
production to 850 mg/l. During batch cultivation of the strain in a
bioreactor, a maximum of 4 g/l of dry cell mass, 1.8 g/l of
siderophore and 20 mg/l of DAPG was achieved when glycerol was
15 g/l and C/N ratio was maintained at 12.5. In case of intermittent
feeding of fresh medium during fed-batch cultivation, the dry cell
mass was increased to 25 g/l with improved production of DAPG to
70 mg/l.
Abstract: Monoculture and mixed cultures of microalgae and
the oleaginous yeast for microbial oil productions were investigated
using sugarcane juice as carbon substrate. The monoculture of yeast
Torulaspora maleeae Y30, Torulaspora globosa YU5/2 grew faster
than that of microalgae Chlorella sp. KKU-S2. In monoculture of T.
maleeae Y30, a biomass of 8.267g/L with lipid yield of 0.920g/L
were obtained, while 8.333g/L of biomass with lipid yield of
1.141g/L were obtained for monoculture of T. globosa YU5/2. A
biomass of 1.933g/L with lipid yield of 0.052g/L was found for
monoculture of Chlorella sp. KKU-S2. The biomass concentration in
the mixed culture of the oleaginous yeast with microalgae increased
faster and was higher compared with that in the monocultures. A
biomass of 8.733g/L with lipid yield of 1.564g/L was obtained for a
mixed culture of T. maleeae Y30 with Chlorella sp. KKU-S2, while
8.010g/L of biomass with lipid yield of 2.424g/L was found for
mixed culture of T. globosa YU5/2 with Chlorella sp. KKU-S2.
Maximum cell yield coefficient (YX/S, g/L) was found of 0.323 in
monoculture of Chlorella sp. KKU-S2 but low level of both specific
yield of lipid (YP/X, g lipid/g cells) of 0.027 and volumetric lipid
production rate (QP, g/L/d) of 0.003 were observed. While, maximum
YP/X (0.303), QP (0.105) and maximum process product yield (YP/S,
0.061) were obtained in mixed culture of T. globosa YU5/2 with
Chlorella sp. KKU-S2. The results obtained from the study shows
that mixed culture of yeast with microalgae is a desirable cultivation
process for microbial oil production.
Abstract: Two different superhydrophobic surfaces were
elaborated and their oil repellency behavior was evaluated using
several liquid with different surface tension. A silicone rubber/SiO2
nanocomposite coated (A) on aluminum substrate by “spin-coating"
and the sample B was an anodized aluminum surface covered by
Teflon-like coating. A high static contact angle about ∼162° was
measured for two prepared surfaces on which the water droplet rolloff.
Scanning electron microscopy (SEM) showed the presence of
micro/nanostructures for both sample A and B similar to that of lotus
leaf.
However the sample A presented significantly different behaviour
of wettability against the low surface tension liquid. Sample A has
been wetted totally by oil (dodecan) droplet while sample B showed
oleophobic behaviour. Oleophobic property of Teflon like coating
can be contributed to the presence of CF2 and CF3 functional group
which was shown by XPS analysis.