Assessment of Photodynamic Therapy for Staphylococcus Aureus Infected Wounds using Diffuse Reflectance Spectrometry

In this paper we evaluated the efficacy of photodynamic treatment of infected wounds on pig animal model by diffuse reflectance spectrometry. The study was conducted on fifteen wounds contaminated with Staphylococcus aureus bacteria that were incubated for 30 min with methylene blue solution (c = 3.3 x 10-3 M) and exposed to laser radiations (λ = 670 nm, P = 15 mW) for 15 min. The efficiency of photodynamic inactivation of bacteria was evaluated by microbiological exams and diffuse reflectance spectrometry. The results of the microbiological exams showed that the bacterial concentration has decreased from 6.93±0.138 logCFU/ml to 3.12±0.108 logCFU/ml. The spectral examination showed that the diffuse reflectance of wounds contaminated with Staphylococcus aureus has decreased from 5.06±0.036 % to 3.36±0.025 %. In conclusion, photodynamic therapy is an effective method for the treatment of infected wounds and there is a correlation between the CFU count and diffuse reflectance.

IEEE 802.11 b and g WLAN Propagation Model using Power Density Measurements at ESPOL

This paper describes the development of a WLAN propagation model, using Spectral Analyzer measurements. The signal is generated by two Access Points (APs) on the base floor at the administrative Communication School of ESPOL building. In general, users do not have a Q&S reference about a wireless network; however, this depends on the level signal as a function of frequency, distance and other path conditions between receiver and transmitter. Then, power density of the signal decrease as it propagates through space and data transfer rate is affected. This document evaluates and implements empirical mathematical formulation for the characterization of WLAN radio wave propagation on two aisles of the building base floor.

Optimum Radio Capacity Estimation of a Single-Cell Spread Spectrum MIMO System under Rayleigh Fading Conditions

In this paper, the problem of estimating the optimal radio capacity of a single-cell spread spectrum (SS) multiple-inputmultiple- output (MIMO) system operating in a Rayleigh fading environment is examined. The optimisation between the radio capacity and the theoretically achievable average channel capacity (in the sense of information theory) per user of a MIMO single-cell SS system operating in a Rayleigh fading environment is presented. Then, the spectral efficiency is estimated in terms of the achievable average channel capacity per user, during the operation over a broadcast time-varying link, and leads to a simple novel-closed form expression for the optimal radio capacity value based on the maximization of the achieved spectral efficiency. Numerical results are presented to illustrate the proposed analysis.

Enhancement of m-FISH Images using Spectral Unmixing

Breast carcinoma is the most common form of cancer in women. Multicolour fluorescent in-situ hybridisation (m-FISH) is a common method for staging breast carcinoma. The interpretation of m-FISH images is complicated due to two effects: (i) Spectral overlap in the emission spectra of fluorochrome marked DNA probes and (ii) tissue autofluorescence. In this paper hyper-spectral images of m-FISH samples are used and spectral unmixing is applied to produce false colour images with higher contrast and better information content than standard RGB images. The spectral unmixing is realised by combinations of: Orthogonal Projection Analysis (OPA), Alterating Least Squares (ALS), Simple-to-use Interactive Self-Modeling Mixture Analysis (SIMPLISMA) and VARIMAX. These are applied on the data to reduce tissue autofluorescence and resolve the spectral overlap in the emission spectra. The results show that spectral unmixing methods reduce the intensity caused by tissue autofluorescence by up to 78% and enhance image contrast by algorithmically reducing the overlap of the emission spectra.

A Modified Speech Enhancement Using Adaptive Gain Equalizer with Non linear Spectral Subtraction for Robust Speech Recognition

In this paper we present an enhanced noise reduction method for robust speech recognition using Adaptive Gain Equalizer with Non linear Spectral Subtraction. In Adaptive Gain Equalizer method (AGE), the input signal is divided into a number of subbands that are individually weighed in time domain, in accordance to the short time Signal-to-Noise Ratio (SNR) in each subband estimation at every time instant. Instead of focusing on suppression the noise on speech enhancement is focused. When analysis was done under various noise conditions for speech recognition, it was found that Adaptive Gain Equalizer method algorithm has an obvious failing point for a SNR of -5 dB, with inadequate levels of noise suppression for SNR less than this point. This work proposes the implementation of AGE when coupled with Non linear Spectral Subtraction (AGE-NSS) for robust speech recognition. The experimental result shows that out AGE-NSS performs the AGE when SNR drops below -5db level.

Fluid Structure Interaction Induced by Liquid Slosh in Partly Filled Road Tankers

The liquid cargo contained in a partly-filled road tank vehicle is prone to dynamic slosh movement when subjected to external disturbances. The slosh behavior has been identified as a significant factor impairing the safety of liquid cargo transportation. The laboratory experiments have been conducted for analyzing fluid slosh in partly filled tanks. The experiment results measured under forced harmonic excitations reveal the three-dimensional nature of the fluid motion and coupling between the lateral and longitudinal fluid slosh at resonance. Several spectral components are observed for the transient slosh forces, which can be associated with the excitation, resonance, and beat frequencies. The peak slosh forces and moments in the vicinity of resonance are significantly larger than those of the equivalent rigid mass. Due to the nature of coupling between sloshing fluid and vehicle body, the issue of the dynamic fluid-structure interaction is essential in the analysis of tank-vehicle dynamics. A dynamic pitch plane model of a Tridem truck incorporated the fluid slosh dynamics is developed to analyze the fluid-vehicle interaction under the straight-line braking maneuvers. The results show that the vehicle responses are highly associated with the characteristics of fluid slosh force and moment.

Recursive Wiener-Khintchine Theorem

Power Spectral Density (PSD) computed by taking the Fourier transform of auto-correlation functions (Wiener-Khintchine Theorem) gives better result, in case of noisy data, as compared to the Periodogram approach. However, the computational complexity of Wiener-Khintchine approach is more than that of the Periodogram approach. For the computation of short time Fourier transform (STFT), this problem becomes even more prominent where computation of PSD is required after every shift in the window under analysis. In this paper, recursive version of the Wiener-Khintchine theorem has been derived by using the sliding DFT approach meant for computation of STFT. The computational complexity of the proposed recursive Wiener-Khintchine algorithm, for a window size of N, is O(N).

Solvatochromic Shift and Estimation of Dipole Moment of Quinine Sulphate Dication

Absorption and fluorescence spectra of quinine sulphate (QSD) have been recorded at room temperature in wide range of solvents of different polarities. The ground-state dipole moment of QSD was obtained from quantum mechanical calculations and the excited state dipole moment of QSD was estimated from Bakhshiev-s and Kawski-Chamma-Viallet-s equations by means of solvatochromic shift method. Higher value of dipole moment is observed for excited state as compared to the corresponding ground state value and this is attributed to the more polar excited state of QSD.

The Projection Methods for Computing the Pseudospectra of Large Scale Matrices

The projection methods, usually viewed as the methods for computing eigenvalues, can also be used to estimate pseudospectra. This paper proposes a kind of projection methods for computing the pseudospectra of large scale matrices, including orthogonalization projection method and oblique projection method respectively. This possibility may be of practical importance in applications involving large scale highly nonnormal matrices. Numerical algorithms are given and some numerical experiments illustrate the efficiency of the new algorithms.

Unsteady Transonic Aerodynamic Analysis for Oscillatory Airfoils using Time Spectral Method

This research proposes an algorithm for the simulation of time-periodic unsteady problems via the solution unsteady Euler and Navier-Stokes equations. This algorithm which is called Time Spectral method uses a Fourier representation in time and hence solve for the periodic state directly without resolving transients (which consume most of the resources in a time-accurate scheme). Mathematical tools used here are discrete Fourier transformations. It has shown tremendous potential for reducing the computational cost compared to conventional time-accurate methods, by enforcing periodicity and using Fourier representation in time, leading to spectral accuracy. The accuracy and efficiency of this technique is verified by Euler and Navier-Stokes calculations for pitching airfoils. Because of flow turbulence nature, Baldwin-Lomax turbulence model has been used at viscous flow analysis. The results presented by the Time Spectral method are compared with experimental data. It has shown tremendous potential for reducing the computational cost compared to the conventional time-accurate methods, by enforcing periodicity and using Fourier representation in time, leading to spectral accuracy, because results verify the small number of time intervals per pitching cycle required to capture the flow physics.

Two-Dimensional Solitary Wave Solution to the Quadratic Nonlinear Schrdinger Equation

The solitary wave solution of the quadratic nonlinear Schrdinger equation is determined by the iterative method called Petviashvili method. This solution is also used for the initial condition for the time evolution to study the stability analysis. The spectral method is applied for the time evolution.

A Robust Extrapolation Method for Curtailed Aperture Reconstruction in Acoustic Imaging

Acoustic Imaging based sound localization using microphone array is a challenging task in digital-signal processing. Discrete Fourier transform (DFT) based near-field acoustical holography (NAH) is an important acoustical technique for sound source localization and provide an efficient solution to the ill-posed problem. However, in practice, due to the usage of small curtailed aperture and its consequence of significant spectral leakage, the DFT could not reconstruct the active-region-of-sound (AROS) effectively, especially near the edges of aperture. In this paper, we emphasize the fundamental problems of DFT-based NAH, provide a solution to spectral leakage effect by the extrapolation based on linear predictive coding and 2D Tukey windowing. This approach has been tested to localize the single and multi-point sound sources. We observe that incorporating extrapolation technique increases the spatial resolution, localization accuracy and reduces spectral leakage when small curtail aperture with a lower number of sensors accounts.

Trispectral Analysis of Voiced Sounds Defective Audition and Tracheotomisian Cases

This paper presents the cepstral and trispectral analysis of a speech signal produced by normal men, men with defective audition (deaf, deep deaf) and others affected by tracheotomy, the trispectral analysis based on parametric methods (Autoregressive AR) using the fourth order cumulant. These analyses are used to detect and compare the pitches and the formants of corresponding voiced sounds (vowel \a\, \i\ and \u\). The first results appear promising, since- it seems after several experimentsthere is no deformation of the spectrum as one could have supposed it at the beginning, however these pathologies influenced the two characteristics: The defective audition influences to the formants contrary to the tracheotomy, which influences the fundamental frequency (pitch).

On Detour Spectra of Some Graphs

The Detour matrix (DD) of a graph has for its ( i , j) entry the length of the longest path between vertices i and j. The DD-eigenvalues of a connected graph G are the eigenvalues for its detour matrix, and they form the DD-spectrum of G. The DD-energy EDD of the graph G is the sum of the absolute values of its DDeigenvalues. Two connected graphs are said to be DD- equienergetic if they have equal DD-energies. In this paper, the DD- spectra of a variety of graphs and their DD-energies are calculated.

H-ARQ Techniques for Wireless Systems with Punctured Non-Binary LDPC as FEC Code

This paper presents the H-ARQ techniques comparison for OFDM systems with a new family of non-binary LDPC codes which has been developed within the EU FP7 DAVINCI project. The punctured NB-LDPC codes have been used in a simulated model of the transmission system. The link level performance has been evaluated in terms of spectral efficiency, codeword error rate and average number of retransmissions. The NB-LDPC codes can be easily and effective implemented with different methods of the retransmission needed if correct decoding of a codeword failed. Here the Optimal Symbol Selection method is proposed as a Chase Combining technique.

On Use of Semiconductor Detector Arrays on COMPASS Tokamak

Semiconductor detector arrays are widely used in high-temperature plasma diagnostics. They have a fast response, which allows observation of many processes and instabilities in tokamaks. In this paper, there are reviewed several diagnostics based on semiconductor arrays as cameras, AXUV photodiodes (referred often as fast “bolometers") and detectors of both soft X-rays and visible light installed on the COMPASS tokamak recently. Fresh results from both spring and summer campaigns in 2012 are introduced. Examples of the utilization of the detectors are shown on the plasma shape determination, fast calculation of the radiation center, two-dimensional plasma radiation tomography in different spectral ranges, observation of impurity inflow, and also on investigation of MHD activity in the COMPASS tokamak discharges.

Extinct Ponds: Potential for Increasing Landscape Retention Capacity?

The restoration of extinct ponds is considered as one of ways to gain new retention capacities for water which is getting much more important issue with respect to expected impacts of a climate change. However, there are also other pressures on the landscape which must be all taken into consideration when making a decision on the possible restoration of extinct ponds. The research presented here focuses besides others on the restoration of former ponds which could be important for both the flood protection and drought impacts prevention. The first step of the methodology development for the assessment of such areas is the assessment of their present state. In this paper, the results of land use types assessment for 22 localities are presented. These results confirm the assumption that the most present land use type in such areas is the permanent grassland. However, the spectra of land use types present in extinct pond areas is very diverse and include besides others also airport areas and industry.

Formant Tracking Linear Prediction Model using HMMs for Noisy Speech Processing

This paper presents a formant-tracking linear prediction (FTLP) model for speech processing in noise. The main focus of this work is the detection of formant trajectory based on Hidden Markov Models (HMM), for improved formant estimation in noise. The approach proposed in this paper provides a systematic framework for modelling and utilization of a time- sequence of peaks which satisfies continuity constraints on parameter; the within peaks are modelled by the LP parameters. The formant tracking LP model estimation is composed of three stages: (1) a pre-cleaning multi-band spectral subtraction stage to reduce the effect of residue noise on formants (2) estimation stage where an initial estimate of the LP model of speech for each frame is obtained (3) a formant classification using probability models of formants and Viterbi-decoders. The evaluation results for the estimation of the formant tracking LP model tested in Gaussian white noise background, demonstrate that the proposed combination of the initial noise reduction stage with formant tracking and LPC variable order analysis, results in a significant reduction in errors and distortions. The performance was evaluated with noisy natual vowels extracted from international french and English vocabulary speech signals at SNR value of 10dB. In each case, the estimated formants are compared to reference formants.

Quantification of Peptides based on Isotope Dilution Surface Enhanced Raman Scattering

This study aims to demonstrate the quantification of peptides based on isotope dilution surface enhanced Raman scattering (IDSERS). SERS spectra of phenylalanine (Phe), leucine (Leu) and two peptide sequences TGQIFK (T13) and YSFLQNPQTSLCFSESIPTPSNR (T6) as part of the 22-kDa human growth hormone (hGH) were obtained on Ag-nanoparticle covered substrates. On the basis of the dominant Phe and Leu vibrational modes, precise partial least squares (PLS) prediction models were built enabling the determination of unknown T13 and T6 concentrations. Detection of hGH in its physiological concentration in order to investigate the possibility of protein quantification has been achieved.

Response Spectrum Transformation for Seismic Qualification Testing

Seismic qualification testing for equipments to be mounted on upper storeys of buildings is very demanding in terms of floor spectra. The latter is characterized by high accelerations amplitudes within a narrow frequency band. This article presents a method which permits to cover specified required response spectra beyond the shaking table capability by amplifying the acceleration amplitudes at an appropriate frequency range using a physical intermediate mounted on the platform of the shaker.