Abstract: This research work presents the surface
thermodynamics approach to M-TB/HIV-Human sputum
interactions. This involved the use of the Hamaker coefficient
concept as a surface energetics tool in determining the interaction
processes, with the surface interfacial energies explained using van
der Waals concept of particle interactions. The Lifshitz derivation for
van der Waals forces was applied as an alternative to the contact
angle approach which has been widely used in other biological
systems. The methodology involved taking sputum samples from
twenty infected persons and from twenty uninfected persons for
absorbance measurement using a digital Ultraviolet visible
Spectrophotometer. The variables required for the computations with
the Lifshitz formula were derived from the absorbance data. The
Matlab software tools were used in the mathematical analysis of the
data produced from the experiments (absorbance values). The
Hamaker constants and the combined Hamaker coefficients were
obtained using the values of the dielectric constant together with the
Lifshitz Equation. The absolute combined Hamaker coefficients
A132abs and A131abs on both infected and uninfected sputum samples
gave the values of A132abs = 0.21631x10-21Joule for M-TB infected
sputum and Ã132abs = 0.18825x10-21Joule for M-TB/HIV infected
sputum. The significance of this result is the positive value of the
absolute combined Hamaker coefficient which suggests the existence
of net positive van der waals forces demonstrating an attraction
between the bacteria and the macrophage. This however, implies that
infection can occur. It was also shown that in the presence of HIV,
the interaction energy is reduced by 13% conforming adverse effects
observed in HIV patients suffering from tuberculosis.
Abstract: This paper presents results of the survey regarding the
awareness about HIV/AIDS among HIV-infected individuals. A
questionnaire covering various aspects of HIV-infection was
conducted among 110 HIV-infected individuals who attended the
G.A. Zaharyan Moscow Tuberculosis Clinic, Department for
treatment of TB patients with HIV. The questionnaire included
questions about modes of HIV transmission and preventive measures
against HIV/AIDS, as well as questions about age, gender, education
and employment status. The survey revealed that the respondents in
the whole had a good knowledge regarding modes of HIV
transmission and preventive measures against HIV/AIDS: about
83,6% male respondents and 85,7% female respondents gave an
accurate answers regarding the HIV-infection. However, the
overwhelming majority of the study participants, that is, 88,5% men
and 98% women, was quite ignorant about the risk of acquiring HIV
through saliva and toothbrush of HIV-infected individual. Though
that risk is rather insignificant, it is still biologically possible. And
this gap in knowledge needs to be filled. As the study showed another
point of concern was the fact, that despite the knowledge of HIV
transmission risk through unprotected sex about 40% percent of HIVpositive
men and 25% of HIV-positive women did not insist on using
condoms with their sexual partners. These findings indicate that there
are still some aspects about HIV-infection which needed to be
clarified and explained through more detailed and specific
educational programs.
Abstract: This paper presents the design, development and
evaluation of an application prototype developed to support
tuberculosis (TB) patients’ treatment adherence. The system makes
use of graphics and voice reminders as opposed to text messaging to
encourage patients to follow their medication routine. To evaluate the
effect of the prototype applications, participants were given mobile
phones on which the reminder system was installed. Thirty-eight
people, including TB health workers and patients from Zanzibar,
Tanzania, participated in the evaluation exercises. The results
indicate that the participants found the mobile image-based
application is useful to support TB treatment. All participants
understood and interpreted the intended meaning of every image
correctly. The study findings revealed that the use of a mobile visualbased
application may have potential benefit to support TB patients
(both literate and illiterate) in their treatment processes.
Abstract: Tuberculosis (TB) remains a leading cause of
infectious mortality. It is primarily transmitted by the respiratory
route, individuals with active disease may infect others through
airborne particles which releases when they cough, talk, or sing and
subsequently inhale by others. In order to study the effect of the
Bacilli Calmette-Guerin (BCG) vaccine after vaccination of TB
patient, a Vaccinated Susceptible Infected and Recovered (VSIR)
mathematical model is being developed to achieve the desired
objectives. The mathematical model, so developed, shall be used to
quantify the effect of BCG Vaccine to protect the immigrant young
adult person. Moreover, equations are to be established for the
disease endemic and free equilibrium states and subsequently utilized
in disease stability analysis. The stability analysis will give a
complete picture of disease annihilation from the total population if
the total removal rate from the infectious group should be greater
than total number of dormant infections produced throughout
infectious period.
Abstract: Potato (Solanum tuberosum L.) is one of the major
vegetable crops that are grown world –wide because of its economic
importance. This experiment investigated the effect of local sands
(River Base, Al-Ekader and Karbala) on number and total weight of
minitubers. Statistical analysis revealed that there were no significant
differences among sand cultures in number of stem/plant, chlorophyll
index and tubers dry weight. River Base sand had the highest plant
height (74.9 cm), leaf number/plant number (39.3), leaf area (84.4
dcm2⁄plant), dry weight/plant (26.31), tubers number/plant (8.5),
tubers weight/plant (635.53 gm) and potato tuber yields/trove (28.60
kg), whereas the Karbala sand had lower performance. All the
characters had positive and significant correlation with yields except
the traits number of stem and tuber dry weight.
Abstract: Effect of biopesticide from wood vinegar and
extracted substances from 3 medicinal plants such as: non taai yak
(Stemona tuberosa Lour), boraphet (Tinospora crispa Mier) and
derris (Derris elliptica Roxb) were tested on the age five years of
pomelo. The selected pomelo was carried out for insects’ pest control
and its quality. The experimental site was located at farmer’s orchard
in Phichit Province, Thailand. This study was undertaken during the
drought season (December to March). The extracted from plants and
wood vinegar were evaluated in 6 treatments: 1) water as control; 2)
wood vinegar; 3) S. tuberosa Lour; 4) T. crispa Mier; 5) D. elliptica
Roxb; 6) mixed (wood vinegar + S. tuberosa Lour + T. crispa Mier +
D. elliptica Roxb). The experiment was RCB with 6 treatments and 3
replications per treatment. The results showed that T. crispa Mier was
the highest effectiveness for reduction population of thrips
(Scirtothrips dorsalis Hood) and citrus leaf miner (Phyllocnistis
citrella Stainton) at 14.10 and 15.37 respectively, followed by
treatment of mixed, D. elliptica Roxb, S. tuberosa Lour and wood
vinegar with significance different. Additionally, T. crispa Mier
promoted the high quality of harvested pomelo in term of thickness
of skin at 12.45 mm and S. tuberosa Lour gave the high quality of the
pomelo in term of firmness (276.5 kg/cm2) and brix (11.0%).
Abstract: Ambrosia trifida L. is designated as invasive alien
species by the Act on the Conservation and Use of Biodiversity by the
Ministry of Environment, Korea. The purpose of present paper was to
investigate the inhibitory effects of aqueous extracts of A.trifida on the
development of root hairs of Triticum aestivum L., and Allium
tuberosum Rottler ex Spreng and the electrophoretic protein patterns of
their radicles. The development of root hairs was inhibited by
increasing of aqueous extract concentrations. Through SDS-PAGE,
the electrophoretic protein bands of extracted proteins from their
radicles were appeared in controls, but protein bands of specific
molecular weight disappeared or weakened in treatments. In
conclusion, inhibitory effects of A. trifida made two receptor species
changed morphologically, and at the molecular level in early growth
stage.
Abstract: We present a deterministic model which describes
the dynamics of tuberculosis in Algerian population where the
vaccination program with BCG is in place since 1969 and where
the WHO recommendations regarding the DOTS (directly-observed
treatment, short course) strategy are in application. The impact
of an intervention program, targeting recently infected people
among all close contacts of active cases and their treatment to
prevent endogenous reactivation, on the incidence of tuberculosis,
is investigated. We showed that a widespread treatment of latently
infected individuals for some years is recommended to shift from
higher to lower equilibrium state and thereafter relaxation is
recommended.
Abstract: New diagnostic tools are urgently needed to interrupt the transmission of tuberculosis and multidrug-resistant tuberculosis. The microscopic-observation drug-susceptibility (MODS) assay is a rapid, accurate and simple liquid culture method to detect multidrug-resistant tuberculosis (MDR-TB). MODS were evaluated to determine a lower and same concentration of isoniazid and rifampin for detection of MDR-TB. Direct drug-susceptibility testing was performed with the use of the MODS assay. Drug-sensitive control strains were tested daily. The drug concentrations that used for both isoniazid and rifampin were at the same concentration: 0.16, 0.08 and 0.04μg per milliliter. We tested 56 M. tuberculosis clinical isolates and the control strains M. tuberculosis H37RV. All concentration showed same result. Of 53 M. tuberculosis clinical isolates, 14 were MDR-TB, 38 were susceptible with isoniazid and rifampin, 1 was resistant with isoniazid only. Drug-susceptibility testing was performed with the use of the proportion method using Mycobacteria Growth Indicator Tube (MGIT) system as reference. The result of MODS assay using lower concentration was significance (P
Abstract: Altered drug binding may be an important factor in isoniazid (INH) resistance, rather than major changes in the enzyme’s activity as a catalase or peroxidase (KatG). The identification of structural or functional defects in the mutant KatGs responsible for INH resistance remains as an area to be explored. In this connection, the differences in the binding affinity between wild-type (WT) and mutants of KatG were investigated, through the generation of three mutants of KatG, Ser315Thr [S315T], Ser315Asn [S315N], Ser315Arg [S315R] and a WT [S315]) with the help of software-MODELLER. The mutants were docked with INH using the software-GOLD. The affinity is lower for WT than mutant, suggesting the tight binding of INH with the mutant protein compared to WT type. These models provide the in silico evidence for the binding interaction of KatG with INH and implicate the basis for rationalization of INH resistance in naturally occurring KatG mutant strains of Mycobacterium tuberculosis.
Abstract: Pyrazinamide (PZA) is among the first-line pro-drugs in the tuberculosis (TB) combination chemotherapy used to treat Mycobacterium tuberculosis. Numerous reports have suggested that hepatotoxicity due to pyrazinamide in patients is due to inappropriate dosing. It is, therefore necessary to develop sensitive and reliable techniques for determining the PZA metabolic profile of diagnosed patients promptly and at point-of-care. This study reports the determination of PZA based on nanobiosensor systems developed from disuccinimidyl octanedioate modified Cytochrome P450-2E1 (CYP2E1) electrodeposited on gold substrates derivatised with (poly(8-anilino-1-napthalene sulphonic acid) PANSA/PVP-AgNPs nanocomposites. The rapid and sensitive amperometric PZA detection gave a dynamic linear range of 2µM to 16µM revealing a
limit of detection of 0.044µM and a sensitivity of 1.38µA/µM. The Michaelis-Menten parameters; KM, KM app and IMAX were calculated to be 6.0µM, 1.41µM and 1.51x10-6 A, respectively, indicating a nanobiosensor suitable for use in serum.
Abstract: M. paratuberculosis is a slow growing mycobactin dependent mycobacterial species known to be the causative agent of Johne’s disease in all species of domestic ruminants worldwide. JD is characterized by gradual weight loss; decreased milk production. Excretion of the organism may occur for prolonged periods (1 to 2.5 years) before the onset of clinical disease. In recent years researchers focus on identification a specific antigen of MAP to use in diagnosis test and preparation of effective vaccine. In this paper, for production of polyclonal antibody against proteins of Mycobacterium avium paratuberculosis cell well a rabbit immunization at a certain time period with antigen. After immunization of the animal, rabbit was bleeded for producing enriched serum. Antibodies were purification with ion exchange chromatography. For exact measurement of interaction, western blotting test was used that this study demonstrated sharp bands appears in nitrocellulose paper and specific bands were 50 and 150 KD molecular weight. These were indicating immunodominant proteins.
Abstract: Modelling is a widely used tool to facilitate the evaluation of disease management. The interest of epidemiological models lies in their ability to explore hypothetical scenarios and provide decision makers with evidence to anticipate the consequences of disease incursion and impact of intervention strategies.
All models are, by nature, simplification of more complex systems. Models that involve diseases can be classified into different categories depending on how they treat the variability, time, space, and structure of the population. Approaches may be different from simple deterministic mathematical models, to complex stochastic simulations spatially explicit.
Thus, epidemiological modelling is now a necessity for epidemiological investigations, surveillance, testing hypotheses and generating follow-up activities necessary to perform complete and appropriate analysis.
The state of the art presented in the following, allows us to position itself to the most appropriate approaches in the epidemiological study.
Abstract: There is long history of konjac tubers being used as a cure for certain diseases in China and Japan. Konjac flour is prepared from konjac tubers and it contains high concentration of glucomannan. Konjac Glucomannan (KGM) is dietary fiber and the role of which has been demonstrated in weight reduction, lowering blood cholesterol and sugar level, promoting intestinal activity etc. Konjac glucomanan has a property of swelling by absorbing water, more than a hundred times its own weight. Therefore it helps increasing weight of feces, water content of feces, and promotes satiety feeling. Mode of actions of dietary fibre as laxatives agents includes holding water inside the bowel lumen, inhibition of water absorption in the colon and stimulating colonic motility. Number of fecal pellets did not effected in rats were fed on 300 and 600 mg/kg of konjac flour, as well as constipated control and Dulcolax treatment. Water content, weight of fecal pellets and gastrointestinal transit ratio were higher in rats treated with 600 mg/kg than 300 mg/kg of konjac flour. Rats were administered with Dulcolax showed the highest gastrointestinal transit ratio, followed by 600 mg/kg konjac flour. The lowest feed consumption was noted in 600 mg/kg konjac flour diet group.
Abstract: There has been studied the distribution character of potato stem nematode (Ditylenchus destructor Thorne, 1945) on the potato fields in four municipalities (Tsalka, Bolnisi, Marneuli, Gardabani) of Kvemo Kartli (Eastern Georgia).
As a result of scientific research there is stated the extensiveness of pathogens invasion, accompanying composition of fauna species, environmental groups of populations and quantity.
During the research process in the studied ecosystems there were registered 160 forms of free-living and Phyto-parasitic nematodes, from which 118 forms are determined as species and 42 as genus.
It was found that in almost the entire studied ecosystem there is dominated pathogenic nematodes Ditylenchus destructor. The large number of exemplars (almost uncountable) was found in tubers material of Bolnisi and Gardabani.
Abstract: Cattle manure and mineral fertilizers are two source
of Nitrogen, which can affect the growth and quantity of potato. In
this research the effects of the use of cattle manure (5, 10, 15 and 20
ton ha-1), Nitrogen fertilizer (50, 100 and 150 kg N ha-1) and their
interaction on potato growth were evaluated during field
experiments in 2008 with the help of Randomized Complete Block
(RCB) with the factorial arrangement of three experimental
replications in Iran. At the 75 th day after emergence, dry weight of
Shoots, leaf area index (LAI) and plant height were recorded. Results
showed that, dry weight of Shoots, LAI and plant height increased
linearly and very significantly in response to the application of
manure and Nitrogen fertilizer. While the interaction between
manure and Nitrogen fertilizer just on the LAI and plant height was
significant, somehow the maximum amount of plant height( 73 cm)
was obtained by using 150 kg Nitrogen + 15 tons of manure per
hectare, and maximum LAI ( 5.36) was obtained by using 150 kg
Nitrogen + 20 tons of manure per hectare. Also in this experiment
maximum tuber yield (36.8 tons ha-1) was obtained by the utilization
of 150 kg Nitrogen per hectare + 20 tons manure.
Abstract: The study aimed to investigate characteristics of
vegetative tissue for taxonomic purpose and possibly trend of waste
application in industry. Stems and branches of 15 species in Solanum
found in Thailand were prepared for fiber and examined by light
microscopy. Microstructural characteristic data of fiber i.e. fiber
length and width, fiber lumen diameter and fiber cell wall thickness
were recorded. The longest average fiber cell length (>3.9 mm.) were
obtained in S. lycopersicum L. and S. tuberosum L. Fiber cells from
S. lycopersicum also revealed the widest average diameter of whole
cell and its lumen at >45.5 μm and >29 μm respectively. However
fiber cells with thickest wall of > 9.6 μm were belonged to the
ornamental tree species, S. wrightii Benth. The results showed that
the slenderness ratio, Runkel ratio, and flexibility coefficient, with
potentially suitable for feedstock in paper industry fell in 4 exotic
species, i.e. Solanumamericanum L., S. lycopersicum, S.
seaforthianum Andr., and S. tuberosum L
Abstract: To study the effect of suitable methods for
propagation of True Potato Seed (TPS) progenies, transplant and
selection of the best progenies, a factorial experiment base on a
randomized complete block design was carried out in the research
field of Sahneh region, Kermanshah, Iran during 2009-2010. Five
selective progenies from CIP (International Potato Center) including
CIP.994013, CIP.994002, CIP.994014, CIP.888006, and
CIP.994001 and two transplant preparation methods (Paper pot
preparation for mechanical cultivation and preparation in transplant
trays for manual cultivation) were studied in three replications.
Results showed that different progenies had no significant effect on
plant height (cm) and tuber yield (t ha-1), whereas had a significant
effect on number of tubers per unit area (m2). There was significant
difference between transplant preparation methods for plant height
and tuber yield. The interaction effect of progenies and transplant
preparation method was not significant for these traits. CIP.888006
progeny and paper pot preparation method produced the highest
tuber yields. Also CIP.994002 and CIP.994014 progenies considered
as the best progenies under paper pot preparation method due to high
yields.
Abstract: The in vitro culture procedure of purple nutsedge
(Cyperus rotundus L.) for multiple shoot induction and tuber
formation was established. Multiple shoots were significantly
induced from a single shoot of about 0.5 – 0.8 cm long, on Murashige
and Skoog (MS) medium supplemented with 4.44 μM 6-
benzyladinine (BA) alone or in combination with 2.85 μM 1-
indoleacetic acid (IAA), providing 17.6 and 15.3 shoots per explant
with 31.2 and 27.5 leaves per explant, respectively, within 6 weeks of
culturing. Moreover, MS medium supplemented with 4.44 μM BA
and 2.85 μM IAA was suitable for tuber induction, obtaining 5.9
tubers with 3.4 rhizomes per explant. In combination with ancymidol
and higher concentration of sucrose, 11.1 μM BA and 60 g/L sucrose
or 11.1 μM BA, 7.8 μM ancymidol and 60 g/L sucrose induced 3.5
tubers with 1.6 rhizomes or 3.5 tubers without rhizome, respectively.
However, MS medium containing 3.9 or 7.8 μM ancymidol in
combination with either 60 or 80 g/L sucrose enchanced significant
root formation at 20.9 – 23.6 roots per explant.
Abstract: Meat and meat products for human consumption are one of main sources of protein, amino acids, fatty acids, vitamins, and minerals. Popular variety of meat product is meatballs, which can be enriched with valuable product – Jerusalem artichoke powder, made from dried and grinded Jerusalem artichoke tubers, it is raw material with low-calorie, low fat, rich in dietary fibres, minerals, and vitamins. The results of this study indicate that that people could accept the new product - meatballs with Jerusalem artichoke powder and Jerusalem artichoke powder is suitable for meatballs preparation, in result them is possible to improve meatballs sensory and physical properties.