Abstract: Agriculture is the backbone of economy of Pakistan
and cotton is the major agricultural export and supreme source of raw
fiber for our textile industry. To combat severe problems of insect
and weed, combination of three genes namely Cry1Ac, Cry2A and
EPSPS genes was transferred in locally cultivated cotton variety
MNH-786 with the use of Agrobacterium mediated genetic
transformation. The present study focused on the molecular screening
of transgenic cotton plants at T3 generation in order to confirm
integration and expression of all three genes (Cry1Ac, Cry2A and
EPSP synthase) into the cotton genome. Initially, glyphosate spray
assay was used for screening of transgenic cotton plants containing
EPSP synthase gene at T3 generation. Transgenic cotton plants which
were healthy and showed no damage on leaves were selected after 07
days of spray. For molecular analysis of transgenic cotton plants in
the laboratory, the genomic DNA of these transgenic cotton plants
were isolated and subjected to amplification of the three genes. Thus,
seventeen out of twenty (Cry1Ac gene), ten out of twenty (Cry2A
gene) and all twenty (EPSP synthase gene) were produced positive
amplification. On the base of PCR amplification, ten transgenic plant
samples were subjected to protein expression analysis through
ELISA. The results showed that eight out of ten plants were actively
expressing the three transgenes. Real-time PCR was also done to
quantify the mRNA expression levels of Cry1Ac and EPSP synthase
gene. Finally, eight plants were confirmed for the presence and active
expression of all three genes at T3 generation.
Abstract: This study examines analytically the effect of tsunami loads on reinforced concrete (RC) frame buildings. The impact of tsunami wave loads and waterborne objects are analyzed using a typical substandard full-scale two-story RC frame building tested as part of the EU-funded Ecoleader project. The building was subjected to shake table tests in bare condition, and subsequently strengthened using Carbon Fiber Reinforced Polymers (CFRP) composites and retested. Numerical models of the building in both bare and CFRP-strengthened conditions are calibrated in DRAIN-3DX software to match the test results. To investigate the response of wave loads and impact forces, the numerical models are subjected to nonlinear dynamic analyses using force time-history input records. The analytical results are compared in terms of displacements at the floors and at the “impact point” of a boat. The results show that the roof displacement of the CFRP-strengthened building reduced by 63% when compared to the bare building. The results also indicate that strengthening only the mid-height of the impact column using CFRP is more effective at reducing damage when compared to strengthening other parts of the column. Alternative solutions to mitigate damage due to tsunami loads are suggested.
Abstract: The air transport impact on environment is more than
ever a limitative obstacle to the aeronautical industry continuous
growth. Over the last decades, considerable effort has been carried
out in order to obtain quieter aircraft solutions, whether by changing
the original design or investigating more silent maneuvers. The
noise propagated by rotating surfaces is one of the most important
sources of annoyance, being present in most aerial vehicles. Bearing
this is mind, CEIIA developed a new computational chain for
noise prediction with in-house software tools to obtain solutions in
relatively short time without using excessive computer resources. This
work is based on the new acoustic tool, which aims to predict the
rotor noise generated during steady and maneuvering flight, making
use of the flexibility of the C language and the advantages of GPU
programming in terms of velocity. The acoustic tool is based in the
Formulation 1A of Farassat, capable of predicting two important
types of noise: the loading and thickness noise. The present work
describes the most important features of the acoustic tool, presenting
its most relevant results and framework analyses for helicopters and
UAV quadrotors.
Abstract: Biofuels production has come forth as a future
technology to combat the problem of depleting fossil fuels. Bio-based
ethanol production from enzymatic lignocellulosic biomass
degradation serves an efficient method and catching the eye of
scientific community. High cost of the enzyme is the major obstacle
in preventing the commercialization of this process. Thus main
objective of the present study was to optimize composition of
medium components for enhancing cellulase production by newly
isolated strain of Bacillus tequilensis. Nineteen factors were taken
into account using statistical Plackett-Burman Design. The significant
variables influencing the cellulose production were further employed
in statistical Response Surface Methodology using Central
Composite Design for maximizing cellulase production. The
optimum medium composition for cellulase production was: peptone
(4.94 g/L), ammonium chloride (4.99 g/L), yeast extract (2.00 g/L),
Tween-20 (0.53 g/L), calcium chloride (0.20 g/L) and cobalt chloride
(0.60 g/L) with pH 7, agitation speed 150 rpm and 72 h incubation at
37oC. Analysis of variance (ANOVA) revealed high coefficient of
determination (R2) of 0.99. Maximum cellulase productivity of 11.5
IU/ml was observed against the model predicted value of 13 IU/ml.
This was found to be optimally active at 60oC and pH 5.5.
Abstract: The paper deals with the diagnostics of steel roof
structure of the winter sports halls built in 1970 year. The necessity
of the diagnostics has been given by the requirement to the evaluation
design of this structure, which has been caused by the new situation
in the field of the loadings given by the validity of the European
Standards in the Czech Republic from 2010 year. Due to these
changes in the normative rules, in practice existing structures are
gradually subjected to the evaluation design and depending on its
results to the strengthening or reconstruction, respectively. Steel roof
is composed of plane truss main girders, purlins and bracings and the
roof structure is supported by two arch main girders with the span of
L = 84 m. The in situ diagnostics of the roof structure was oriented to
the following parts: (i) determination and evaluation of the actual
material properties of used steel and (ii) verification of the actual
dimensions of the structural members. For the solution the nondestructive
methods have been used for in situ measurement. For the
indicative determination of steel strengths the modified method based
on the determination of Rockwell’s hardness has been used. For the
verification of the member’s dimensions (thickness of hollow
sections) the ultrasound method has been used. This paper presents
the results obtained using these testing methods and their evaluation,
from the viewpoint of the usage for the subsequent static assessment
and design evaluation of the existing structure. For the comparison,
the examples of the similar evaluations realized for steel structures of
the stadiums in Olomouc and Jihlava cities are briefly illustrated, too.
Abstract: The restrained construction zoning, an important part
in the urban master plan, is a necessary planning tool to control the city
sprawl, to guarantee the reservation implementation of the various
types of protective elements, and to realize the storage of the essential
urban spatial resources. Simultaneously, owing to the diverse
constitutes of restrained construction area and the various stakeholders
involved in, its planning requires an overall consideration of all
elements from the perspective of coordination+, balance and
practicability to deal with the problems and conflicts in this process.
Taking Yangzijin Ecological Restrained Construction Area in
Yangzhou as an example, this study analyzes all the potential actors,
agencies and stakeholders in this restrained construction area, as well
as the relevant conflicts between each other. Besides, this study tries to
build up a planning procedure based on the framework of governance
theory, and proposes a possible planning method that combines
"rigidity" and "flexibility" to protect the ecological limitation
boundary, to take every interest into account, and to promote economic
development in a harmonious society.
Abstract: This study evaluated the use of raw or processed
Prosopis juliflora (Meskit) pods as a major ingredient in a formulated
ration to provide an alternative non-conventional concentrate for
livestock feeding in Oman. Dry Meskit pods were reduced to lengths
of 0.5- 1.0 cm to ensure thorough mixing into three diets. Meskit
pods were subjected to two types of treatments; roasting and soaking.
They were roasted at 150оC for 30 minutes using a locally-made
roasting device (40 kg barrel container rotated by electric motor and
heated by flame gas cooker). Chopped pods were soaked in tap water
for 24 hours and dried for 2 days under the sun with frequent turning.
The Meskit-pod-based diets (MPBD) were formulated and pelleted
from 500 g/kg ground Meskit pods, 240 g/kg wheat bran, 200 g/kg
barley grain, 50 g/kg local dried sardines and 10 g/kg of salt. Twenty
four 10 months-old intact Omani male lambs with average body
weight of 27.3 kg (± 0.5 kg) were used in a feeding trial for 84 days.
They were divided (on body weight basis) and allocated to four diet
combination groups. These were: Rhodes grass hay (RGH) plus a
general ruminant concentrate (GRC); RGH plus raw Meskit pods
(RMP) based concentrate; RGH plus roasted Meskit pods (ROMP)
based concentrate; RGH plus soaked Meskit pods (SMP) based
concentrate Daily feed intakes and bi-weekly body weights were
recorded. MPBD had higher contents of crude protein (CP), acid
detergent fibre (ADF) and neutral detergent fibre (NDF) than the
GRC. Animals fed various types of MPBD did not show signs of ill
health. There was a significant effect of feeding ROMP on the
performance of Omani sheep compared to RMP and SMP. The
ROMP fed animals had similar performance to those fed the GRC in
terms of feed intake, body weight gain and feed conversion ratio
(FCR).This study indicated that roasted Meskit pods based diet may
be used instead of the commercial concentrate for feeding Omani
sheep without adverse effects on performance. It offers a cheap
alternative source of protein and energy for feeding Omani sheep.
Also, it might help in solving the spread impact of Meskit trees,
maintain the ecosystem and helping in preserving the local tree
species.
Abstract: The aim of this paper is to perform experimental
modal analysis (EMA) of reinforced concrete (RC) square slabs.
EMA is the process of determining the modal parameters (Natural
Frequencies, damping factors, modal vectors) of a structure from a
set of frequency response functions FRFs (curve fitting). Although,
experimental modal analysis (or modal testing) has grown steadily in
popularity since the advent of the digital FFT spectrum analyzer in
the early 1970’s, studying all types of members and materials using
such method have not yet been well documented. Therefore, in this
work, experimental tests were conducted on RC square slab
specimens of dimensions 600mm x 600mmx 40mm. Experimental
analysis was based on freely supported boundary condition.
Moreover, impact testing as a fast and economical means of finding
the modes of vibration of a structure was used during the
experiments. In addition, Pico Scope 6 device and MATLAB
software were used to acquire data, analyze and plot Frequency
Response Function (FRF). The experimental natural frequencies
which were extracted from measurements exhibit good agreement
with analytical predictions. It is showed that EMA method can be
usefully employed to investigate the dynamic behavior of RC slabs.
Abstract: Social Media (SM) is websites increasingly popular
and built to allow people to express themselves and to interact
socially with others. Most SMT are dominated by youth particularly
College students. The proliferation of popular social media tools,
which can accessed from any communication devices has become
pervasive in the lives of today’s student life. Connecting traditional
education to social media tools are a relatively new era and any
collaborative tool could be used for learning activities. This study
focuses (i) how the social media tools are useful for the learning
activities of the students of faculty of medicine in King Khalid
University (ii) whether the social media affects the collaborative
learning with interaction among students, among course instructor,
their engagement, perceived ease of use and perceived ease of
usefulness (TAM) (iii) overall, the students satisfy with this
collaborative learning through Social media.
Abstract: An investigation into Cahn-Hilliard equation was
carried out through numerical simulation to identify a possible phase
separation for one and two dimensional domains. It was observed that
this equation can reproduce important mass fluxes necessary for
phase separation within the miscibility gap and for coalescence of
particles.
Abstract: This paper presents small signal stability study carried
over the 140-Bus, 31-Machine, 5-Area MEPE system and validated
on free and open source software: PSAT. Well-established linearalgebra
analysis, eigenvalue analysis, is employed to determine the
small signal dynamic behavior of test system. The aspects of local
and interarea oscillations which may affect the operation and
behavior of power system are analyzed. Eigenvalue analysis is carried
out to investigate the small signal behavior of test system and the
participation factors have been determined to identify the
participation of the states in the variation of different mode shapes.
Also, the variations in oscillatory modes are presented to observe the
damping performance of the test system.
Abstract: The 3D body movement signals captured during
human-human conversation include clues not only to the content of
people’s communication but also to their culture and personality.
This paper is concerned with automatic extraction of this information
from body movement signals. For the purpose of this research, we
collected a novel corpus from 27 subjects, arranged them into groups
according to their culture. We arranged each group into pairs and
each pair communicated with each other about different topics.
A state-of-art recognition system is applied to the problems of
person, culture, and topic recognition. We borrowed modeling,
classification, and normalization techniques from speech recognition.
We used Gaussian Mixture Modeling (GMM) as the main technique
for building our three systems, obtaining 77.78%, 55.47%, and
39.06% from the person, culture, and topic recognition systems
respectively. In addition, we combined the above GMM systems with
Support Vector Machines (SVM) to obtain 85.42%, 62.50%, and
40.63% accuracy for person, culture, and topic recognition
respectively.
Although direct comparison among these three recognition
systems is difficult, it seems that our person recognition system
performs best for both GMM and GMM-SVM, suggesting that intersubject
differences (i.e. subject’s personality traits) are a major
source of variation. When removing these traits from culture and
topic recognition systems using the Nuisance Attribute Projection
(NAP) and the Intersession Variability Compensation (ISVC)
techniques, we obtained 73.44% and 46.09% accuracy from culture
and topic recognition systems respectively.
Abstract: An experiment was conducted to determine the effect
of pollination on seed quality of rapeseed in Chitwan, Nepal during
2012-2013. The experiment was designed in Randomized Complete
Block with four replications and five treatments. The rapeseed plots
were caged with mosquito nets at 10% flowering except natural
pollination. Two-framed colonies of Apis mellifera L. and Apis
cerana F. were introduced separately for pollination, and control plot
caged without pollinators. The highest germination percent was
observed on Apis cerana F. pollinated plot seeds (90.50%
germination) followed by Apis mellifera L. pollinated plots (87.25 %)
and lowest on control plots (42.00% germination) seeds. Similarly,
seed test weight of Apis cerana F. pollinated plots (3.22 gm/ 1000
seed) and Apis mellifera L. pollinated plots (2.93 gm/1000 seed) were
and lowest on control plots (2.26 gm/ 1000 seed) recorded. Likewise,
oil content was recorded highest on pollinated by Apis cerana F.
(36.1%) followed by pollinated by Apis mellifera L. (35.4%) and
lowest on control plots (32.8%). This study clearly indicated
pollination increases the seed quality of rapeseed and therefore,
management of honeybee is necessary for producing higher quality of
rapeseed under Chitwan condition.
Abstract: The legends about “user-friendly” and “easy-to-use”
birotical tools (computer-related office tools) have been spreading
and misleading end-users. This approach has led us to the extremely
high number of incorrect documents, causing serious financial losses
in the creating, modifying, and retrieving processes. Our research
proved that there are at least two sources of this underachievement:
(1) The lack of the definition of the correctly edited, formatted
documents. Consequently, end-users do not know whether their
methods and results are correct or not. They are not aware of their
ignorance. They are so ignorant that their ignorance does not allow
them to realize their lack of knowledge. (2) The end-users’ problem
solving methods. We have found that in non-traditional programming
environments end-users apply, almost exclusively, surface approach
metacognitive methods to carry out their computer related activities,
which are proved less effective than deep approach methods.
Based on these findings we have developed deep approach
methods which are based on and adapted from traditional
programming languages. In this study, we focus on the most popular
type of birotical documents, the text based documents. We have
provided the definition of the correctly edited text, and based on this
definition, adapted the debugging method known in programming.
According to the method, before the realization of text editing, a
thorough debugging of already existing texts and the categorization
of errors are carried out. With this method in advance to real text
editing users learn the requirements of text based documents and also
of the correctly formatted text.
The method has been proved much more effective than the
previously applied surface approach methods. The advantages of the
method are that the real text handling requires much less human and
computer sources than clicking aimlessly in the GUI (Graphical User
Interface), and the data retrieval is much more effective than from
error-prone documents.
Abstract: PLA emerged as a promising polymer because of its
property as a compostable, biodegradable thermoplastic made from
renewable sources. PLA can be polymerized from monomers
(Lactide or Lactic acid) obtained by fermentation processes from
renewable sources such as corn starch or sugarcane. For PLA
synthesis, ring opening polymerization (ROP) of Lactide monomer is
one of the preferred methods. In the literature, the technique mainly
developed for ROP of PLA is based on metal/bimetallic catalyst (Sn,
Zn and Al) or other organic catalysts in suitable solvent. However,
the PLA synthesized using such catalysts may contain trace elements
of the catalyst which may cause toxicity. This work estimated the
usefulness and drawbacks of using different catalysts as well as effect
of alternative energies and future aspects for PLA production.
Abstract: Geometric and mechanical properties all influence the
resistance of RC structures and may, in certain combination of
property values, increase the risk of a brittle failure of the whole
system.
This paper presents a statistical and probabilistic investigation on
the resistance of RC beams designed according to Eurocodes 2 and 8,
and subjected to multiple failure modes, under both the natural
variation of material properties and the uncertainty associated with
cross-section and transverse reinforcement geometry. A full
probabilistic model based on JCSS Probabilistic Model Code is
derived. Different beams are studied through material nonlinear
analysis via Monte Carlo simulations. The resistance model is
consistent with Eurocode 2. Both a multivariate statistical evaluation
and the data clustering analysis of outcomes are then performed.
Results show that the ultimate load behaviour of RC beams
subjected to flexural and shear failure modes seems to be mainly
influenced by the combination of the mechanical properties of both
longitudinal reinforcement and stirrups, and the tensile strength of
concrete, of which the latter appears to affect the overall response of
the system in a nonlinear way. The model uncertainty of the
resistance model used in the analysis plays undoubtedly an important
role in interpreting results.
Abstract: The study examined the effect of Bonny Light whole
crude oil (WC) and its water soluble fraction (WSF) on the activities
of antioxidant enzymes (catalase (CAT) and superoxide dismutase
(SOD)) and crude mitochondria ATPases in the radicle of
germinating bean (Vigna unguiculata). The percentage germination,
level of lipid peroxidation, antioxidant enzyme and mitochondria
Ca2+ and Mg2+ ATPase activities were measured in the radicle of
bean after 7, 14 and 21 days post germination. Viable bean seeds
were planted in soils contaminated with 10ml, 25ml and 50ml of
whole crude oil (WC) and its water soluble fraction (WSF) to obtain
2, 5 and 10% v/w crude oil contamination. There was dose dependent
reduction of the number of bean seeds that germinated in the
contaminated soils compared with control (p
Abstract: This review emphasizes the effectiveness of men’s
participation in preventing domestic violence, and whether nonviolent
(NV) boys’ and men’s perceptions of intimate partner
violence (IPV) prevention programs affect their involvement. The
main goals of this assessment were to investigate (1) how NV men
engaged in anti-violence prevention programs that empower women,
(2) what were the possible perceptions of NV men involved in
prevention programs (3) how to identify effective approaches and
strategies that encouraged NV men to become involved in prevention
programs. This critical review also included the overview of
prevention programs such as: The Mentors in Violence Prevention
Programs (MVP), The White Ribbon Campaign (WRC), and
Domestic Violence Prevention Enhancement and Leadership through
Alliances (DELTA). The review suggested that (1) the expanding
prevention programs need to reach more macro settings such as work
place, faith-based and other community based organizations, and (2)
territory prevention programs should expand through addressing the
long-term effects of violence.
Abstract: Since columns are the most important elements of the
structures, failure of one column in a critical location can cause a
progressive collapse. In this respect, the repair and strengthening of
columns is a very important subject to reduce the building failure and
to keep the columns capacity. Twenty columns with different
parameters is tested and analysis. Eleven typical confined reinforced
concrete (RC) columns with different types of techniques are
assessment. And also, four confined concrete columns with plastic
tube (PVC) are tested with and with four paralleling tested of
unconfined plain concrete. The techniques of confined RC columns
are mortar strengthening, Steel rings strengthening, FRP
strengthening. Moreover, the technique of confined plain concrete
(PC) column is used PVC tubes. The columns are tested under
uniaxial compressive loads studied the effect of confinement on the
structural behavior of circular RC columns. Test results for each
column are presented in the form of crack patterns, stress-strain
curves. Test results show that confining of the RC columns using
different techniques of strengthening results significant improvement
of the general behavior of the columns and can used in construction.
And also, tested confined PC columns with PVC tubes results shown
that the confined PC with PVC tubes can be used in economical
building. The theoretical model for predicted column capacity is
founded with experimental factor depends on the confined techniques
used and the strain reduction.
Abstract: This study carried out comparative seismic
performance of reinforced concrete frames infilled by masonry walls
with different heights. Partial and fully infilled reinforced concrete
frames were modeled for the research objectives and the analysis
model for a bare reinforced concrete frame was also established for
comparison. Non–linear static analyses for the studied frames were
performed to investigate their structural behavior under extreme
seismic loads and to find out their collapse mechanism. It was
observed from analysis results that the strengths of the partial infilled
reinforced concrete frames are increased and their ductilities are
reduced, as infilled masonry walls are higher. Especially, reinforced
concrete frames with higher partial infilled masonry walls would
experience shear failures. Non–linear dynamic analyses using 10
earthquake records show that the bare and fully infilled reinforced
concrete frame present stable collapse mechanism while the reinforced
concrete frames with partially infilled masonry walls collapse in more
brittle manner due to short-column effects.