Supplementation of Vascular Endothelial Growth Factor during in vitro Maturation of Porcine Cumulus Oocyte Complexes and Subsequent Developmental Competence after Parthenogenesis and in vitro Fertilization

In mammalian reproductive tract, the oviduct secretes huge number of growth factors and cytokines that create an optimal micro-environment for the initial stages of preimplantation embryos. Secretion of these growth factors is stage-specific. Among them, VEGF is a potent mitogen for vascular endothelium and stimulates vascular permeability. Apart from angiogenesis, VEGF in the oviduct may be involved in regulating the oocyte maturation and subsequent developmental process during embryo production in vitro. In experiment 1, to evaluate the effect of VEGF during IVM of porcine COC and subsequent developmental ability after PA and SCNT. The results from these experiments indicated that maturation rates among the different VEGF concentrations were not significant different. In experiment 2, total intracellular GSH concentrations of oocytes matured with VEGF (5-50 ng/ml) were increased significantly compared to a control and VEGF group (500 ng/ml). In experiment 3, the blastocyst formation rates and total cell number per blastocyst after parthenogenesis of oocytes matured with VEGF (5-50 ng/ml) were increased significantly compared to a control and VEGF group (500 ng/ml). Similarly, in experiment 4, the blastocyst formation rate and total cell number per blastocyst after SCNT and IVF of oocytes matured with VEGF (5 ng/ml) were significantly higher than that of oocytes matured without VEGF group. In experiment 5, at 10 hour after the onset of IVF, pronuclear formation rate was evaluated. Monospermy was significantly higher in VEGF-matured oocytes than in the control, and polyspermy and sperm penetration per oocyte were significantly higher in the control group than in the VEGFmatured oocytes. Supplementation with VEGF during IVM significantly improved male pronuclear formation as compared with the control. In experiment 6, type III cortical granule distribution in oocytes was more common in VEGF-matured oocytes than in the control. In conclusion, the present study suggested that supplementation of VEGF during IVM may enhance the developmental potential of porcine in vitro embryos through increase of the intracellular GSH level, higher MPN formation and increased fertilization rate as a consequence of an improved cytoplasmic maturation.

Pedometer Development Utilizing an Accelerometer Sensor

This paper develops a pedometer with a three-axis acceleration sensor that can be placed with any angle. The proposed pedometer measures the number of steps while users walk, jog or run. It can be worn on users’ waistband or placed within pocket or backpack. The work address to improve on the general pedometers, which can only be used in a single direction or can only count of steps without the continuous exercise judgment mechanism. Finally, experimental results confirm the superior performance of the proposed pedometer.

A Comparative Study of the Effectiveness of Trained Inspectors in Different Workloads between Feed Forward and Feedback Training

Objective of this study was to study and compare the effectiveness of inspectors who had different workloads for feed forward and feedback training. The visual search task was simulated to search for specified alphabets called defects. These defects were included of four alphabets in Thai and English such as s ภ, ถ, X, and V with different background. These defects were combined in the specified alphabets and were given the different three backgrounds i.e., Thai, English, and mixed English and Thai alphabets. Sixty students were chosen as a sample in this study and test for final selection subject. Finally, five subjects were taken into testing process. They were asked to search for defects after they were provided basic information. Experiment design was used factorial design and subjects were trained for feed forward and the feedback training. The results show that both trainings were affected on mean search time. It was also found that the feedback training can increase the effectiveness of visual inspectors rather than the feed forward training significantly different at the level of .05

Optimizing Electrospinning Parameters for Finest Diameter of Nano Fibers

Nano fibers produced by electrospinning are of industrial and scientific attention due to their special characteristics such as long length, small diameter and high surface area. Applications of electrospun structures in nanotechnology are included tissue scaffolds, fibers for drug delivery, composite reinforcement, chemical sensing, enzyme immobilization, membrane-based filtration, protective clothing, catalysis, solar cells, electronic devices and others. Many polymer and ceramic precursor nano fibers have been successfully electrospun with diameters in the range from 1 nm to several microns. The process is complex so that fiber diameter is influenced by various material, design and operating parameters. The objective of this work is to apply genetic algorithm on the parameters of electrospinning which have the most significant effect on the nano fiber diameter to determine the optimum parameter values before doing experimental set up. Effective factors including initial polymer concentration, initial jet radius, electrical potential, relaxation time, initial elongation, viscosity and distance between nozzle and collector are considered to determine finest diameter which is selected by user.

Removal of CO2 and H2S using Aqueous Alkanolamine Solusions

This work presents a theoretical investigation of the simultaneous absorption of CO2 and H2S into aqueous solutions of MDEA and DEA. In this process the acid components react with the basic alkanolamine solution via an exothermic, reversible reaction in a gas/liquid absorber. The use of amine solvents for gas sweetening has been investigated using process simulation programs called HYSYS and ASPEN. We use Electrolyte NRTL and Amine Package and Amines (experimental) equation of state. The effects of temperature and circulation rate and amine concentration and packed column and murphree efficiency on the rate of absorption were studied. When lean amine flow and concentration increase, CO2 and H2S absorption increase too. With the improvement of inlet amine temperature in absorber, CO2 and H2S penetrate to upper stages of absorber and absorption of acid gases in absorber decreases. The CO2 concentration in the clean gas can be greatly influenced by the packing height, whereas for the H2S concentration in the clean gas the packing height plays a minor role. HYSYS software can not estimate murphree efficiency correctly and it applies the same contributions in all diagrams for HYSYS software. By improvement in murphree efficiency, maximum temperature of absorber decrease and the location of reaction transfer to the stages of bottoms absorber and the absorption of acid gases increase.

CFD Simulation and Validation of Flow Pattern Transition Boundaries during Moderately Viscous Oil-Water Two-Phase Flow through Horizontal Pipeline

In the present study, computational fluid dynamics (CFD) simulation has been executed to investigate the transition boundaries of different flow patterns for moderately viscous oil-water (viscosity ratio 107, density ratio 0.89 and interfacial tension of 0.032 N/m.) two-phase flow through a horizontal pipeline with internal diameter and length of 0.025 m and 7.16 m respectively. Volume of Fluid (VOF) approach including effect of surface tension has been employed to predict the flow pattern. Geometry and meshing of the present problem has been drawn using GAMBIT and ANSYS FLUENT has been used for simulation. A total of 47037 quadrilateral elements are chosen for the geometry of horizontal pipeline. The computation has been performed by assuming unsteady flow, immiscible liquid pair, constant liquid properties, co-axial flow and a T-junction as entry section. The simulation correctly predicts the transition boundaries of wavy stratified to stratified mixed flow. Other transition boundaries are yet to be simulated. Simulated data has been validated with our own experimental results.

Multivariate High Order Fuzzy Time Series Forecasting for Car Road Accidents

In this paper, we have presented a new multivariate fuzzy time series forecasting method. This method assumes mfactors with one main factor of interest. History of past three years is used for making new forecasts. This new method is applied in forecasting total number of car accidents in Belgium using four secondary factors. We also make comparison of our proposed method with existing methods of fuzzy time series forecasting. Experimentally, it is shown that our proposed method perform better than existing fuzzy time series forecasting methods. Practically, actuaries are interested in analysis of the patterns of causalities in road accidents. Thus using fuzzy time series, actuaries can define fuzzy premium and fuzzy underwriting of car insurance and life insurance for car insurance. National Institute of Statistics, Belgium provides region of risk classification for each road. Thus using this risk classification, we can predict premium rate and underwriting of insurance policy holders.

Mathematical Modelling of Single Phase Unity Power Factor Boost Converter

An optimal control strategy based on simple model, a single phase unity power factor boost converter is presented with an evaluation of first order differential equations. This paper presents an evaluation of single phase boost converter having power factor correction. The simple discrete model of boost converter is formed and optimal control is obtained, digital PI is adopted to adjust control error. The method of instantaneous current control is proposed in this paper for its good tracking performance of dynamic response. The simulation and experimental results verified our design.

Development of a New Piezoelectrically Actuated Micropump for Liquid and Gas

This paper aims to present the design, fabrication and test of a novel piezoelectric actuated, check-valves embedded micropump having the advantages of miniature size, light weight and low power consumption. This device is designed to pump gases and liquids with the capability of performing the self-priming and bubble-tolerant work mode by maximizing the stroke volume of the membrane as well as the compression ratio via minimization of the dead volume of the micropump chamber and channel. By experiment apparatus setup, we can get the real-time values of the flow rate of micropump, the displacement of the piezoelectric actuator and the deformation of the check valve, simultaneously. The micropump with check valve 0.4 mm in thickness obtained higher output performance under the sinusoidal waveform of 120 Vpp. The micropump achieved the maximum pumping rates of 42.2 ml/min and back pressure of 14.0 kPa at the corresponding frequency of 28 and 20 Hz. The presented micropump is able to pump gases with a pumping rate of 196 ml/min at operating frequencies of 280 Hz under the sinusoidal waveform of 120 Vpp.

Real Time Control Learning Game - Speed Race by Learning at the Wheel - Development of Data Acquisition System

Schools today face ever-increasing demands in their attempts to ensure that students are well equipped to enter the workforce and navigate a complex world. Research indicates that computer technology can help support learning, implementation of various experiments or learning games, and that it is especially useful in developing the higher-order skills of critical thinking, observation, comprehension, implementation, comparison, analysis and active attention to activities such as research, field work, simulations and scientific inquiry. The ICT in education supports the learning procedure by enabling it to be more flexible and effective, create a rich and attractive training environment and equip the students with knowledge and potential useful for the competitive social environment in which they live. This paper presents the design, the development, and the results of the evaluation analysis of an interactive educational game which using real electric vehicles - toys (material) on a toy race track. When the game starts each student selects a specific vehicle toy. Then students are answering questionnaires in the computer. The vehicles' speed is related to the percentage of right answers in a multiple choice questionnaire (software). Every question has its own significant value depending of the different level of questionnaire. Via the developed software, each right or wrong answers in questionnaire increase or decrease the real time speed of their vehicle toys. Moreover the rate of vehicle's speed increase or decrease depends on the difficulty level of each question. The aim of the work is to attract the student’s interest in a learning process and also to improve their scores. The developed real time game was tested using independent populations of students of age groups: 8-10, 11-14, 15-18 years. Standard educational and statistical analysis tools were used for the evaluation analysis of the game. Results reveal that students using the developed real time control game scored much higher (60%) than students using a traditional simulation game on the same questionnaire. Results further indicate that student's interest in repeating the developed real time control gaming was far higher (70%) than the interest of students using a traditional simulation game.

Migration of a Drop in Simple Shear Flow at Finite Reynolds Numbers: Size and Viscosity Ratio Effects

The migration of a deformable drop in simple shear flow at finite Reynolds numbers is investigated numerically by solving the full Navier-Stokes equations using a finite difference/front tracking method. The objectives of this study are to examine the effectiveness of the present approach to predict the migration of a drop in a shear flow and to investigate the behavior of the drop migration with different drop sizes and non-unity viscosity ratios. It is shown that the drop deformation depends strongly on the capillary number, so that; the proper non-dimensional number for the interfacial tension is the capillary number. The rate of migration increased with increasing the drop radius. In other words, the required time for drop migration to the centreline decreases. As the viscosity ratio increases, the drop rotates more slowly and the lubrication force becomes stronger. The increased lubrication force makes it easier for the drop to migrate to the centre of the channel. The migration velocity of the drop vanishes as the drop reaches the centreline under viscosity ratio of one and non-unity viscosity ratios. To validate the present calculations, some typical results are compared with available experimental and theoretical data.

Effect of Coolant on Cutting Forces and Surface Roughness in Grinding of CSM GFRP

This paper presents a comparative study on dry and wet grinding through experimental investigation in the grinding of CSM glass fibre reinforced polymer laminates using a pink aluminium oxide wheel. Different sets of experiments were performed to study the effects of the independent grinding parameters such as grinding wheel speed, feed and depth of cut on dependent performance criteria such as cutting forces and surface finish. Experimental conditions were laid out using design of experiment central composite design. An effective coolant was sought in this study to minimise cutting forces and surface roughness for GFRP laminates grinding. Test results showed that the use of coolants reduces surface roughness, although not necessarily the cutting forces. These research findings provide useful economic machining solution in terms of optimized grinding conditions for grinding CSM GFRP.

DACS3: Embedding Individual Ant Behavior in Ant Colony System

Ants are fascinating creatures that demonstrate the ability to find food and bring it back to their nest. Their ability as a colony, to find paths to food sources has inspired the development of algorithms known as Ant Colony Systems (ACS). The principle of cooperation forms the backbone of such algorithms, commonly used to find solutions to problems such as the Traveling Salesman Problem (TSP). Ants communicate to each other through chemical substances called pheromones. Modeling individual ants- ability to manipulate this substance can help an ACS find the best solution. This paper introduces a Dynamic Ant Colony System with threelevel updates (DACS3) that enhance an existing ACS. Experiments were conducted to observe single ant behavior in a colony of Malaysian House Red Ants. Such behavior was incorporated into the DACS3 algorithm. We benchmark the performance of DACS3 versus DACS on TSP instances ranging from 14 to 100 cities. The result shows that the DACS3 algorithm can achieve shorter distance in most cases and also performs considerably faster than DACS.

Automation of Fishhooks Objective Measures

Fishing has always been an essential component of the Polynesians- life. Fishhooks, mostly in pearl shell, found during archaeological excavations are the artifacts related to this activity the most numerous. Thanks to them, we try to reconstruct the ancient techniques of resources exploitation, inside the lagoons and offshore. They can also be used as chronological and cultural indicators. The shapes and dimensions of these artifacts allow comparisons and classifications used in both functional approach and chrono-cultural perspective. Hence it is very important for the ethno-archaeologists to dispose of reliable methods and standardized measurement of these artifacts. Such a reliable objective and standardized method have been previously proposed. But this method cannot be envisaged manually because of the very important time required to measure each fishhook manually and the quantity of fishhooks to measure (many hundreds). We propose in this paper a detailed acquisition protocol of fishhooks and an automation of every step of this method. We also provide some experimental results obtained on the fishhooks coming from three archaeological excavations sites.

Urban Land Cover Change of Olomouc City Using LANDSAT Images

This paper regards the phenomena of intensive suburbanization and urbanization in Olomouc city and in Olomouc region in general for the period of 1986–2009. A Remote Sensing approach that involves tracking of changes in Land Cover units is proposed to quantify the urbanization state and trends in temporal and spatial aspects. It actually consisted of two approaches, Experiment 1 and Experiment 2 which implied two different image classification solutions in order to provide Land Cover maps for each 1986–2009 time split available in the Landsat image set. Experiment 1 dealt with the unsupervised classification, while Experiment 2 involved semi- supervised classification, using a combination of object-based and pixel-based classifiers. The resulting Land Cover maps were subsequently quantified for the proportion of urban area unit and its trend through time, and also for the urban area unit stability, yielding the relation of spatial and temporal development of the urban area unit. Some outcomes seem promising but there is indisputably room for improvements of source data and also processing and filtering.

First Studies of the Influence of Single Gene Perturbations on the Inference of Genetic Networks

Inferring the network structure from time series data is a hard problem, especially if the time series is short and noisy. DNA microarray is a technology allowing to monitor the mRNA concentration of thousands of genes simultaneously that produces data of these characteristics. In this study we try to investigate the influence of the experimental design on the quality of the result. More precisely, we investigate the influence of two different types of random single gene perturbations on the inference of genetic networks from time series data. To obtain an objective quality measure for this influence we simulate gene expression values with a biologically plausible model of a known network structure. Within this framework we study the influence of single gene knock-outs in opposite to linearly controlled expression for single genes on the quality of the infered network structure.

The Potential of Natural Waste (Corn Husk) for Production of Environmental Friendly Biodegradable Film for Seedling

The use of plastic materials in agriculture causes serious hazards to the environment. The introduction of biodegradable materials, which can be disposed directly into the soil can be one possible solution to this problem. In the present research results of experimental tests carried out on biodegradable film fabricated from natural waste (corn husk) are presented. The film was characterized by Fourier transform infrared spectroscopy (FTIR), differential scanning calorimeter (DSC), thermal gravimetric analysis (TGA) and atomic force microscope (AFM) observation. The film is shown to be readily degraded within 7-9 months under controlled soil conditions, indicating a high biodegradability rate. The film fabricated was use to produce biodegradable pot (BioPot) for seedlings plantation. The introduction and the expanding use of biodegradable materials represent a really promising alternative for enhancing sustainable and environmentally friendly agricultural activities.

Application of SDS/LABS in Recovery Improvement from Fractured Models

This work concerns on experimentally investigation of surfactant flooding in fractured porous media. In this study a series of water and surfactant injection processes were performed on micromodels initially saturated with a heavy crude oil. Eight fractured glass micromodels were used to illustrate effects of surfactant types and concentrations on oil recovery efficiency in presence of fractures with different properties i.e. fracture orientation, length and number of fractures. Two different surfactants with different concentrations were tested. The results showed that surfactant flooding would be more efficient by using SDS surfactant aqueous solution and also by locating injection well in a proper position respect to fracture properties. This study demonstrates different physical and chemical conditions that affect the efficiency of this method of enhanced oil recovery.

Graph-based High Level Motion Segmentation using Normalized Cuts

Motion capture devices have been utilized in producing several contents, such as movies and video games. However, since motion capture devices are expensive and inconvenient to use, motions segmented from captured data was recycled and synthesized to utilize it in another contents, but the motions were generally segmented by contents producers in manual. Therefore, automatic motion segmentation is recently getting a lot of attentions. Previous approaches are divided into on-line and off-line, where on-line approaches segment motions based on similarities between neighboring frames and off-line approaches segment motions by capturing the global characteristics in feature space. In this paper, we propose a graph-based high-level motion segmentation method. Since high-level motions consist of several repeated frames within temporal distances, we consider all similarities among all frames within the temporal distance. This is achieved by constructing a graph, where each vertex represents a frame and the edges between the frames are weighted by their similarity. Then, normalized cuts algorithm is used to partition the constructed graph into several sub-graphs by globally finding minimum cuts. In the experiments, the results using the proposed method showed better performance than PCA-based method in on-line and GMM-based method in off-line, as the proposed method globally segment motions from the graph constructed based similarities between neighboring frames as well as similarities among all frames within temporal distances.

Latent Semantic Inference for Agriculture FAQ Retrieval

FAQ system can make user find answer to the problem that puzzles them. But now the research on Chinese FAQ system is still on the theoretical stage. This paper presents an approach to semantic inference for FAQ mining. To enhance the efficiency, a small pool of the candidate question-answering pairs retrieved from the system for the follow-up work according to the concept of the agriculture domain extracted from user input .Input queries or questions are converted into four parts, the question word segment (QWS), the verb segment (VS), the concept of agricultural areas segment (CS), the auxiliary segment (AS). A semantic matching method is presented to estimate the similarity between the semantic segments of the query and the questions in the pool of the candidate. A thesaurus constructed from the HowNet, a Chinese knowledge base, is adopted for word similarity measure in the matcher. The questions are classified into eleven intension categories using predefined question stemming keywords. For FAQ mining, given a query, the question part and answer part in an FAQ question-answer pair is matched with the input query, respectively. Finally, the probabilities estimated from these two parts are integrated and used to choose the most likely answer for the input query. These approaches are experimented on an agriculture FAQ system. Experimental results indicate that the proposed approach outperformed the FAQ-Finder system in agriculture FAQ retrieval.