Abstract: Poly (ethylene glycol) (PEG) molecules attached to surfaces have shown high potential as a protein repellent due to their flexibility and highly water solubility. A quartz crystal microbalance recording frequency and dissipation changes (QCM-D) has been used to study the adsorption from aqueous solutions, of lysozyme and α-lactalbumin proteins (the last with and without calcium) onto modified stainless steel surfaces. Surfaces were coated with poly(ethylene imine) (PEI) and silicate before grafting on PEG molecules. Protein adsorption was also performed on the bare stainless steel surface as a control. All adsorptions were conducted at 23°C and pH 7.2. The results showed that the presence of PEG molecules significantly reduced the adsorption of lysozyme and α- lactalbumin (with calcium) onto the stainless steel surface. By contrast, and unexpected, PEG molecules enhanced the adsorption of α-lactalbumin (without calcium). It is suggested that the PEG -α- lactalbumin hydrophobic interaction plays a dominant role which leads to protein aggregation at the surface for this latter observation. The findings also lead to the general conclusion that PEG molecules are not a universal protein repellent. PEG-on-PEI surfaces were better at inhibiting the adsorption of lysozyme and α-lactalbumin (with calcium) than with PEG-on-silicate surfaces.
Abstract: Self-compacting concrete (SCC), a new kind of high
performance concrete (HPC) have been first developed in Japan in
1986. The development of SCC has made casting of dense
reinforcement and mass concrete convenient, has minimized noise.
Fresh self-compacting concrete (SCC) flows into formwork and
around obstructions under its own weight to fill it completely and
self-compact (without any need for vibration), without any
segregation and blocking. The elimination of the need for
compaction leads to better quality concrete and substantial
improvement of working conditions. SCC mixes generally have a
much higher content of fine fillers, including cement, and produce
excessively high compressive strength concrete, which restricts its
field of application to special concrete only. To use SCC mixes in
general concrete construction practice, requires low cost materials to
make inexpensive concrete.
Rice husk ash (RHA) has been used as a highly reactive
pozzolanic material to improve the microstructure of the interfacial
transition zone (ITZ) between the cement paste and the aggregate in
self compacting concrete. Mechanical experiments of RHA blended
Portland cement concretes revealed that in addition to the pozzolanic
reactivity of RHA (chemical aspect), the particle grading (physical
aspect) of cement and RHA mixtures also exerted significant
influences on the blending efficiency.
The scope of this research was to determine the usefulness of Rice
husk ash (RHA) in the development of economical self compacting
concrete (SCC). The cost of materials will be decreased by reducing
the cement content by using waste material like rice husk ash instead
of.
This paper presents a study on the development of Mechanical
properties up to 180 days of self compacting and ordinary concretes
with rice-husk ash (RHA), from a rice paddy milling industry in
Rasht (Iran). Two different replacement percentages of cement by
RHA, 10%, and 20%, and two different water/cementicious material
ratios (0.40 and 0.35), were used for both of self compacting and
normal concrete specimens. The results are compared with those of
the self compacting concrete without RHA, with compressive,
flexural strength and modulus of elasticity. It is concluded that RHA
provides a positive effect on the Mechanical properties at age after
60 days.
Base of the result self compacting concrete specimens have higher
value than normal concrete specimens in all test except modulus of
elasticity. Also specimens with 20% replacement of cement by RHA
have the best performance.
Abstract: Dextran is a D-glucose polymer which is produced by
Leuconostoc mesenteroides grown in a sucrose-rich media. The
organism was obtained from the Persian Type Culture Collection
(PTCC) and was transferred in MRS broth medium at 30°C and pH
6.8 for 24 h. After preparation of inoculums, organisms were
inoculated into five liquid fermentation media containing either
molasses or cheese whey or different combinations of cheese whey
and molasses. After certain fermentation period, the produced
dextran was separated and dried. Dextran yield was calculated and
significant differences in different media were observed.
Furthermore, FT-IR analysis was performed and the results showed
that there were no significant differences in the produced dextran
structures.
Abstract: In this paper, vapour-liquid critical locus for the
binary system acetone + chloroform was determined experimentally
over the whole range of composition. The critical property
measurements were carried out using a dynamic-synthetic apparatus,
employed in the dynamic mode. The critical points are visually
determined by observing the critical opalescence and the
simultaneous disappearance and reappearance of the meniscus in the
middle of a high-pressure view cell which withstands operations up
to 673K and 20MPa. The experimental critical points measured in
this work were compared to those available in literature.
Abstract: The design of an active leg orthosis for tumble
protection is proposed in this paper. The orthosis would be applied to
assist elders or invalids in rebalancing while they fall unexpectedly.
We observe the regain balance motion of healthy and youthful people,
and find the difference to elders or invalids. First, the physical model
of leg would be established, and we consider the leg motions are
achieve through four joints (phalanx stem, ankle, knee, and hip joint)
and five links (phalanges, talus, tibia, femur, and hip bone). To
formulate the dynamic equations, the coordinates which can clearly
describe the position in 3D space are first defined accordance with the
human movement of leg, and the kinematics and dynamics of the leg
movement can be formulated based on the robotics. For the purpose,
assisting elders and invalids in avoiding tumble, the posture variation
of unbalance and regaining balance motion are recorded by the
motion-capture image system, and the trajectory is taken as the desire
one. Then we calculate the force and moment of each joint based on
the leg motion model through programming MATLAB code. The
results would be primary information of the active leg orthosis design
for tumble protection.
Abstract: Lignocellulosic materials are new targeted source to
produce second generation biofuels like biobutanol. However, this
process is significantly resisted by the native structure of biomass.
Therefore, pretreatment process is always essential to remove
hemicelluloses and lignin prior to the enzymatic hydrolysis.
The goals of pretreatment are removing hemicelluloses and
lignin, increasing biomass porosity, and increasing the enzyme
accessibility. The main goal of this research is to study the important
variables such as pretreatment temperature and time, which can give
the highest total sugar yield in pretreatment step by using dilute
phosphoric acid. After pretreatment, the highest total sugar yield of
13.61 g/L was obtained under an optimal condition at 140°C for 10
min of pretreatment time by using 1.75% (w/w) H3PO4 and at 15:1
liquid to solid ratio. The total sugar yield of two-stage process
(pretreatment+enzymatic hydrolysis) of 27.38 g/L was obtained.
Abstract: High Pressure Raman scattering measurements of KDP:Mn were performed at room temperatures. The X-ray powder diffraction patterns taken at room temperature by Rietveld refinement showed that doped samples of KDP-Mn have the same tetragonal structure of a pure KDP crystal, but with a contraction of the crystalline cell. The behavior of the Raman spectra, in particular the emergence of a new modes at 330 cm-1, indicates that KDP:Mn undergoes a structural phase transition with onset at around 4 GP. First principle density-functional theory (DFT) calculations indicate that tetrahedral rotation with pressure is predominantly around the c crystalline direction. Theoretical results indicates that pressure induced tetrahedral rotations leads to change tetrahedral neighborhood, activating librations/bending modes observed for high pressure phase of KDP:Mn with stronger Raman activity.
Abstract: Quantitative precipitation forecast (QPF) from
atmospheric model as input to hydrological model in an integrated
hydro-meteorological flood forecasting system has been operational
in many countries worldwide. High-resolution numerical weather
prediction (NWP) models with grid cell sizes between 2 and 14 km
have great potential in contributing towards reasonably accurate QPF.
In this study the potential of two NWP models to forecast
precipitation for a flood-prone area in a tropical region is examined.
The precipitation forecasts produced from the Fifth Generation Penn
State/NCAR Mesoscale (MM5) and Weather Research and
Forecasting (WRF) models are statistically verified with the observed
rain in Kelantan River Basin, Malaysia. The statistical verification
indicates that the models have performed quite satisfactorily for low
and moderate rainfall but not very satisfactory for heavy rainfall.
Abstract: Five vegetables (spinach, lettuce, cabbage, tomato, and onion) were freshly harvested from the Alau Dam and Gongulong agricultural areas for the determination of some organochlorine pesticide residues (o, p-DDE, p,p’-DDD, o,p’-DDD, p,p’-DDT, α-BHC, γ-BHC, metoxichlor, lindane, endosulfan dieldrin, and aldrin.) Soil samples were also collected at different depths for the determination of the above pesticides. Samples collection and preparation were conducted using standard procedures. The concentrations of all the pesticides in the soil and vegetable samples were determined using GC/MS SHIMADZU (GC-17A) equipped with electron capture detector (ECD). The highest concentration was that of p,p’-DDD (132.4±13.45µg/g) which was observed in the leaf of cabbage, while the lowest concentration was that of p,p’-DDT (2.34µg/g) was observed in the root of spinach. Similar trends were observed at the Gongulong agricultural area, with p,p’-DDD having the highest concentration of 153.23µg/g in the leaf of cabbage, while the lowest concentration was that of p,p’-DDT (12.45µg/g) which was observed in the root of spinach. α-BHC, γ-BHC, Methoxychlor, and lindane were detected in all the vegetable samples studied. The concentrations of all the pesticides in the soil samples were observed to be higher at a depth of 21-30cm, while the lowest concentrations were observed at a depth of 0-10cm. The concentrations of all the pesticides in the vegetables and soil samples from the two agricultural sites were observed to be at alarming levels, much higher than the maximum residue limits (MRLs) and acceptable daily intake values (ADIs) .The levels of the pesticides observed in the vegetables and soil samples investigated, are of such a magnitude that calls for special attention and laws to regulate the use and circulation of such chemicals. Routine monitoring of pesticide residues in these study areas is necessary for the prevention, control and reduction of environmental pollution, so as to minimize health risks.
Abstract: The technique of inducing micro ecosystem
restoration is one of aquatic ecology engineering methods used to
retrieve the polluted water. Batch scale study, pilot plant study, and
field study were carried out to observe the eutrophication using the
Inducing Ecology Restorative Symbiosis Agent (IERSA) consisting
mainly degraded products by using lactobacillus, saccharomycete,
and phycomycete. The results obtained from the experiments of the
batch scale and pilot plant study allowed us to development the
parameters for the field study. A pond, 5 m to the outlet of a lake,
with an area of 500 m2 and depth of 0.6-1.2 m containing about 500
tons of water was selected as a model. After the treatment with 10
mg IERSA/L water twice a week for 70 days, the micro restoration
mechanisms consisted of three stages (i.e., restoration, impact
maintenance, and ecology recovery experiment after impact). The
COD, TN, TKN, and chlorophyll a were reduced significantly in the
first week. Although the unexpected heavy rain and contaminate
from sewage system might slow the ecology restoration. However,
the self-cleaning function continued and the chlorophyll a reduced
for 50% in one month. In the 4th week, amoeba, paramecium, rotifer,
and red wriggle worm reappeared, and the number of fish flies
appeared up to1000 fish fries/m3. Those results proved that inducing
restorative mechanism can be applied to improve the eutrophication
and to control the growth of algae in the lakes by gaining the selfcleaning
through inducing and competition of microbes. The
situation for growth of fishes also can reach an excellent result due to
the improvement of water quality.
Abstract: Wireless sensor network is formed with the combination of sensor nodes and sink nodes. Recently Wireless sensor network has attracted attention of the research community. The main application of wireless sensor network is security from different attacks both for mass public and military. However securing these networks, by itself is a critical issue due to many constraints like limited energy, computational power and lower memory. Researchers working in this area have proposed a number of security techniques for this purpose. Still, more work needs to be done.In this paper we provide a detailed discussion on security in wireless sensor networks. This paper will help to identify different obstacles and requirements for security of wireless sensor networks as well as highlight weaknesses of existing techniques.
Abstract: In the present study, the response of Nili Ravi buffalo
oocytes to recombinant human follicle stimulating hormone (rhFSH)
(Organon) on meiotic maturation in vitro was examined. Oocytes
were matured in vitro in medium containing either 0 or 0.05 IU/ ml
rhFSH and the stage of nuclear maturation recorded after 24 hours.
The percentage of oocytes in the control group undergoing germinal
vesicle breakdown (GVBD) observed after 24 hours of culture was
29 % whereas as in rhFSH group the percentage was 10 % were at
this stage (P< 0.001).Thus in the presence of rhFSH, a significantly
greater number of oocytes had progressed to the more advanced
stages of nuclear maturation. Indeed, the maturation of GV
(Germinal Vesicle) stage oocytes to the metaphase II (M II) stage
after 24 hours was significantly (P< 0.0001) increased by the
addition of rhFSH (82 % VS 47 %). The percentage of degenerated
oocytes after 24 hours of culture was 24 % in control group, whereas
in rhFSH group the percentage was 8 % after 24 hours. Degeneration
of the oocytes after 24 hours was not significantly (P = 0. 9361)
decreased.
Abstract: Saddlepoint approximations is one of the tools to obtain
an expressions for densities and distribution functions. We approximate
the densities of the observed gaps between the hypopnea events
using the Huzurbazar saddlepoint approximation. We demonstrate the
density of a maximum likelihood estimator in exponential families.
Abstract: The accelerated sonophotocatalytic degradation of
Reactive Red (RR) 120 dye under visible light using dye sensitized
TiO2 activated by ultrasound has been carried out. The effect of
sonolysis, photocatalysis and sonophotocatalysis under visible light
has been examined to study the influence on the degradation rates by
varying the initial substrate concentration, pH and catalyst loading to
ascertain the synergistic effect on the degradation techniques.
Ultrasonic activation contributes degradation through cavitation
leading to the splitting of H2O2 produced by both photocatalysis and
sonolysis. This results in the formation of oxidative species, such as
singlet oxygen (1O2) and superoxide (O2
-●) radicals in the presence of
oxygen. The increase in the amount of reactive radical species which
induce faster oxidation of the substrate and degradation of
intermediates and also the deaggregation of the photocatalyst are
responsible for the synergy observed under sonication. A
comparative study of photocatalysis and sonophotocatalysis using
TiO2, Hombikat UV 100 and ZnO was also carried out.
Abstract: The present study investigates numerically the
phenomenon of vortex-shedding and its suppression in twodimensional
mixed convective flow past a square cylinder under the
joint influence of buoyancy and free-stream orientation with respect
to gravity. The numerical experiments have been conducted at a
fixed Reynolds number (Re) of 100 and Prandtl number (Pr) of 0.71,
while Richardson number (Ri) is varied from 0 to 1.6 and freestream
orientation, α, is kept in the range 0o≤ α ≤ 90o, with 0o
corresponding to an upward flow and 90o representing a cross-flow
scenario, respectively. The continuity, momentum and energy
equations, subject to Boussinesq approximation, are discretized using
a finite difference method and are solved by a semi-explicit pressure
correction scheme. The critical Richardson number, leading to the
suppression of the vortex-shedding (Ric), is estimated by using
Stuart-Landau theory at various free-stream orientations and the
neutral curve is obtained in the Ri-α plane. The neutral curve
exhibits an interesting non-monotonic behavior with Ric first
increasing with increasing values of α upto 45o and then decreasing
till 70o. Beyond 70o, the neutral curve again exhibits a sharp
increasing asymptotic trend with Ric approaching very large values
as α approaches 90o. The suppression of vortex shedding is not
observed at α = 90o (cross-flow). In the unsteady flow regime, the
Strouhal number (St) increases with the increase in Richardson
number.
Abstract: Motor imagery classification provides an important basis for designing Brain Machine Interfaces [BMI]. A BMI captures and decodes brain EEG signals and transforms human thought into actions. The ability of an individual to control his EEG through imaginary mental tasks enables him to control devices through the BMI. This paper presents a method to design a four state BMI using EEG signals recorded from the C3 and C4 locations. Principle features extracted through principle component analysis of the segmented EEG are analyzed using two novel classification algorithms using Elman recurrent neural network and functional link neural network. Performance of both classifiers is evaluated using a particle swarm optimization training algorithm; results are also compared with the conventional back propagation training algorithm. EEG motor imagery recorded from two subjects is used in the offline analysis. From overall classification performance it is observed that the BP algorithm has higher average classification of 93.5%, while the PSO algorithm has better training time and maximum classification. The proposed methods promises to provide a useful alternative general procedure for motor imagery classification
Abstract: The fine structure of supercavitation in the wake of a
symmetrical cylinder is studied with high-speed video cameras. The
flow is observed in a cavitation tunnel at the speed of 8m/sec when the
sidewall and the wake are partially filled with the massive cavitation
bubbles. The present experiment observed that a two-dimensional
ripple wave with a wave length of 0.3mm is propagated in a
downstream direction, and then abruptly increases to a thicker
three-dimensional layer. IR-photography recorded that the wakes
originated from the horseshoe vortexes alongside the cylinder. The
wake was developed to inside the dead water zone, which absorbed the
bubbly wake propelled from the separated vortices at the center of the
cylinder. A remote sensing classification technique (maximum most
likelihood) determined that the surface porosity was 0.2, and the mean
speed in the mixed wake was 7m/sec. To confirm the existence of
two-dimensional wave motions in the interface, the experiments were
conducted at a very low frequency, and showed similar gravity waves
in both the upper and lower interfaces.
Abstract: This research was conducted in the Pua Watershed whereas located in the Upper Nan River Basin in Nan province, Thailand. Nan River basin originated in Nan province that comprises of many tributary streams to produce as inflow to the Sirikit dam provided huge reservoir with the storage capacity of 9510 million cubic meters. The common problems of most watersheds were found i.e. shortage water supply for consumption and agriculture utilizations, deteriorate of water quality, flood and landslide including debris flow, and unstable of riverbank. The Pua Watershed is one of several small river basins that flow through the Nan River Basin. The watershed includes 404 km2 representing the Pua District, the Upper Nan Basin, or the whole Nan River Basin, of 61.5%, 18.2% or 1.2% respectively. The Pua River is a main stream producing all year streamflow supplying the Pua District and an inflow to the Upper Nan Basin. Its length approximately 56.3 kilometers with an average slope of the channel by 1.9% measured. A diversion weir namely Pua weir bound the plain and mountainous areas with a very steep slope of the riverbed to 2.9% and drainage area of 149 km2 as upstream watershed while a mild slope of the riverbed to 0.2% found in a river reach of 20.3 km downstream of this weir, which considered as a gauged basin. However, the major branch streams of the Pua River are ungauged catchments namely: Nam Kwang and Nam Koon with the drainage area of 86 and 35 km2 respectively. These upstream watersheds produce runoff through the 3-streams downstream of Pua weir, Jao weir, and Kang weir, with an averaged annual runoff of 578 million cubic meters. They were analyzed using both statistical data at Pua weir and simulated data resulted from the hydrologic modeling system (HEC–HMS) which applied for the remaining ungauged basins. Since the Kwang and Koon catchments were limited with lack of hydrological data included streamflow and rainfall. Therefore, the mathematical modeling: HEC-HMS with the Snyder-s hydrograph synthesized and transposed methods were applied for those areas using calibrated hydrological parameters from the upstream of Pua weir with continuously daily recorded of streamflow and rainfall data during 2008-2011. The results showed that the simulated daily streamflow and sum up as annual runoff in 2008, 2010, and 2011 were fitted with observed annual runoff at Pua weir using the simple linear regression with the satisfied correlation R2 of 0.64, 062, and 0.59, respectively. The sensitivity of simulation results were come from difficulty using calibrated parameters i.e. lag-time, coefficient of peak flow, initial losses, uniform loss rates, and missing some daily observed data. These calibrated parameters were used to apply for the other 2-ungauged catchments and downstream catchments simulated.
Abstract: Transcription factor p53 has a powerful tumor
suppressing function that is associated with many cancers. However,
p53 of the molecular weight was higher make the limitation across to
skin or cell membrane. Thymidine dinucleotide (pTT), an
oligonucleotide, can activate the p53 transcription factor. pTT is a
hydrophilic and negative charge oligonucleotide, which delivery in to
cell membrane need an appropriate carrier. The aim of this study was
to improve the bioavailability of the nucleotide fragment, thymidine
dinucleotide (pTT), using elasic liposome carriers to deliver the drug
into the skin. The study demonstrate that dioleoylphosphocholine
(DOPC) incorporated with sodium cholate at molar ratio 1:1 can
archived the particle size about 220 nm. This elastic liposome could
penetration through skin from stratum corneum to whole epidermis by
confocal laser scanning microscopy (CLSM). Moreover, we observed
the the slight increase in generation of p53 by western blot.
Abstract: This paper describes the speed sensorless vector control method of the parallel connected induction motor drive fed by a single inverter. Speed and rotor fluxes of the induction motor are estimated by natural observer with load torque adaptation and adaptive rotor flux observer. The performance parameters speed and rotor fluxes are estimated from the measured terminal voltages and currents. Fourth order induction motor model is used and speed is considered as a parameter. The performance of the natural observer is similar to the conventional observer. The speed of an induction motor is estimated by MATLAB simulation under different speed and load conditions. Estimated values along with other measured states are used for closed loop control. The simulation results show that the natural observer is also effective for parallel connected induction motor drive.