Principles of Municipal Sewage Sludge Bioconversion into Biomineral Fertilizer

The efficiency of heavy metals removal from sewage  sludge in bioleaching processes with heterotrophic, chemoautotrophic  (sulphur-oxidizing) sludge cenoses and chemical leaching (in  distilled water, weakly acidic or alkaline medium) was compared.  The efficacy of heavy metals removal from sewage sludge varies  from 83 % (Zn) up to 14 % (Cr) and follows the order: Zn > Mn > Cu  > Ni > Co > Pb > Cr. The advantages of metals bioleaching process  at heterotrophic metabolism were shown. A new process for  bioconversation of sewage sludge into fertilizer at middle  temperatures after partial heavy metals removal was developed. This  process is based on enhancing vital ability of heterotrophic  microorganisms by adding easily metabolized nutrients and synthesis  of metabolites by growing sludge cenoses. These metabolites possess  the properties of heavy metals extractants and flocculants which  provide the enhancement of sludge flocks sedimentation. The process  results in biomineral fertilizer of prolonged action with immobilized  sludge bioelements. The fertilizer satisfies the EU limits for the  sewage sludge of agricultural utilization. High efficiency of the  biomineral fertilizer obtained has been demonstrated in vegetation  experiments.  

Cloning and Functional Characterization of Promoter Elements of the D Hordein Gene from the Barley (Hordeum vulgare L.) by Bioinformatic Tools

The low level of foreign genes expression in transgenic plants is a key factor that limits plant genetic engineering. Because of the critical regulatory activity of the promoters on gene transcription, they are studied extensively to improve the efficiency of the plant transgenic system. The strong constitutive promoters, such as CaMV 35S promoter and Ubiqutin 1 maize are usually used in plant biotechnology research. However the expression level of the foreign genes in all tissues is often undesirable. But using a strong seed-specific promoter to limit gene expression in the seed solves such problems. The purpose of this study is to isolate one of the seed specific promoters of Hordeum vulgare. So one of the common varieties of Hordeum vulgare in Iran was selected and their genomes extracted then the D-Hordein promoter amplified using the specific designed primers. Then the amplified fragment of the insert cloned in an appropriate vector and then transformed to E. coli. At last for the final admission of accuracy the cloned fragments sent for sequencing. Sequencing analysis showed that the cloned fragment DHPcontained motifs; like TATA box, CAAT-box, CCGTCC-box, AMYBOX1 and E-box etc., which constituted the seed-specific promoter activity. The results were compared with sequences existing in data banks. D-Hordein promoters of Alger has 99% similarity at 100 % coverage. The results also showed that D-Hordein promoter of barley and HMW promoter of wheat are too similar.

Ultimate Shear Resistance of Plate Girders Part 2- Höglund Theory

Ultimate shear resistance (USR) of slender plate girders can be predicted theoretically using Cardiff theory or Höglund theory. This paper will be concerned with predicting the USR using Höglund theory and EC3. Two main factors can affect the USR, the panel width “b” and the web depth “d”, consequently, the panel aspect ratio (b/d) has to be identified by limits. In most of the previous study, there is no limit for panel aspect ratio indicated. In this paper theoretical analysis has been conducted to study the effect of (b/d) on the USR. The analysis based on ninety six test results of steel plate girders subjected to shear executed and collected by others. New formula proposed to predict the percentage of the distance between the plastic hinges form in the flanges “c” to panel width “b”. Conservative limits of (c/b) have been suggested to get a consistent value of USR.

Greenhouse Micro Climate Monitoring Based On WSN with Smart Irrigation Technique

Greenhouse is a building, which provides controlled climate conditions to the plants to keep them from external hard conditions. Greenhouse technology gives freedom to the farmer to select any crop type in any time during year. The quality and productivity of plants inside greenhouse is highly dependent on the management quality and a good management scheme is defined by the quality of the information collected from the greenhouse environment. Therefore, Continuous monitoring of environmental variables such as temperature, humidity, and soil moisture gives information to the grower to better understand, how each factor affects growth and how to manage maximal crop productiveness. In this piper, we designed and implemented climate monitoring with irrigation control system based on Wireless Sensor Network (WSN) technology. The designed system is characterized with friendly to use, easy to install by any greenhouse user, multi-sensing nodes, multi-PAN ID, low cast, water irrigation control and low operation complexity. The system consists of two node types (sensing and control) with star topology on one PAN ID. Moreover, greenhouse manager can modifying system parameters such as (sensing node addresses, irrigation upper and lower control limits) by updating corresponding data in SDRAM memory. In addition, the designed system uses 2*16 characters. LCD to display the micro climate parameters values of each plants row inside the greenhouse.

Implementing ALD in Product Development: The Effect of Geometrical Dimensions on Tubular Member Deformation

The product development process has undergone many changes concomitant with world progress in order to produce products that meet customer needs quickly and inexpensively. Analysis-Led Design (ALD) is one of the latest methods in the product development process. It focuses more on up-front engineering, a product quality optimization process that starts early in the conceptual design stage. Product development and manufacturing through ALD utilizes digital tools extensively for design, analysis and product optimization. This study uses computer-aided design (CAD) and finite element method (FEM) simulation to examine the modes of deformation of tubular members under axial loading. A multiple-combination impact absorption tubular member, referred to as a compress–expand member, is proposed as a substitute for the conventional thin-walled cylindrical tube to be used as a vehicle’s crash box. The study of deformation modes is crucial for evaluating the geometrical dimension limits by which a member can absorb energy efficiently.

Examination of the Effect of Air Viscosity on Narrow Acoustic Tubes Using FEM Involving Complex Effective Density and Complex Bulk Modulus

Earphones and headphones, which are compact electro-acoustic transducers, tend to have a lot of acoustic absorption materials and porous materials known as dampers, which often have a large number of extremely small holes and narrow slits to inhibit the resonance of the vibrating system, because the air viscosity significantly affects the acoustic characteristics in such acoustic paths. In order to perform simulations using the finite element method (FEM), it is necessary to be aware of material characteristics such as the impedance and propagation constants of sound absorbing materials and porous materials. The transfer function is widely known as a measurement method for an acoustic tube with such physical properties, but literature describing the measurements at the upper limits of the audible range is yet to be found. The acoustic tube, which is a measurement instrument, must be made narrow, and the distance between the two sets of microphones must be shortened in order to take measurements of acoustic characteristics at higher frequencies. When such a tube is made narrow, however, the characteristic impedance has been observed to become lower than the impedance of air. This paper considers the cause of this phenomenon to be the effect of the air viscosity and describes an FEM analysis of an acoustic tube considering air viscosity to compare to the theoretical formula by including the effect of air viscosity in the theoretical formula for an acoustic tube.

Dynamic Safety-Stock Calculation

In order to ensure a high service level industrial enterprises have to maintain safety-stock that directly influences the economic efficiency at the same time. This paper analyses established mathematical methods to calculate safety-stock. Therefore, the performance measured in stock and service level is appraised and the limits of several methods are depicted. Afterwards, a new dynamic approach is presented to gain an extensive method to calculate safety-stock that also takes the knowledge of future volatility into account.

A Critical Review on the Development of a Theoretical Framework for Managing Environmental Impacts of Construction Project

Construction industry is considered as one of the main contributor of natural resources depletion, responsible for high level pollution and it is one of the attributes that pose climate changes and other environmental threats. A lot of efforts had and have been done to reduce and control these impacts. Project Environmental Management (PEM) includes the processes required to ensure that the impacts of the project execution to the surrounding environment will remain within the limits stated in legal permits. The main aim of most of researches conducted managing Environmental Impacts (EI) is to protect earth planet from pollution. Those researches are presenting four major environmental elements; Environmental Management Systems (EMS), Environmental Design (ED), Environmental Planning (EP) and Environmental Impacts Assessments (EIA). Although everything has been said about environmental management for construction projects, but almost everything remains to be said and therefore to be explored or rediscovered because incontestably, almost everything remains to be done. This paper aimed at reviewing some of what has been said about PEM. Also one of its objectives is to explore and rediscover the whole view of managing the EI problems by proposing a framework that based on the relation between these environmental researches.

Technique for Voltage Control in Distribution System

This paper presents the techniques for voltage control in distribution system. It is integrated in the distribution management system. Voltage is an important parameter for the control of electrical power systems. The distribution network operators have the responsibility to regulate the voltage supplied to consumer within statutory limits. Traditionally, the On-Load Tap Changer (OLTC) transformer equipped with automatic voltage control (AVC) relays is the most popular and effective voltage control device. A static synchronous compensator (STATCOM) may be equipped with several controllers to perform multiple control functions. Static Var Compensation (SVC) is regulation slopes and available margins for var dispatch. The voltage control in distribution networks is established as a centralized analytical function in this paper. 

Issues in the User Interface Design of a Content Rich Vocational Training Application for Digitally Illiterate Users

This paper discusses our preliminary experiences in the design of a user interface of a computerized content-rich vocational training courseware meant for users with little or no computer experience. In targeting a growing population with limited access to skills training of any sort, we faced numerous challenges, including language and cultural differences, resource limits, gender boundaries and, in many cases, the simple lack of trainee motivation. With the size of the unskilled population increasing much more rapidly than the numbers of sufficiently skilled teachers, there is little choice but to develop teaching techniques that will take advantage of emerging computer-based training technologies. However, in striving to serve populations with minimal computer literacy, one must carefully design the user interface to accommodate their cultural, social, educational, motivational and other differences. Our work, which uses computer based and haptic simulation technologies to deliver training to these populations, has provided some useful insights on potential user interface design approaches.

Thermal and Morphological Evaluation of Chemically Pretreated Sugarcane Bagasse

Enzymatic hydrolysis is one of the major steps involved in the conversion from sugarcane bagasse to yield ethanol. This process offers potential for yields and selectivity higher, lower energy costs and milder operating conditions than chemical processes. However, the presence of some factors such as lignin content, crystallinity degree of the cellulose, and particle sizes, limits the digestibility of the cellulose present in the lignocellulosic biomasses. Pretreatment aims to improve the access of the enzyme to the substrate. In this study sugarcane bagasse was submitted chemical pretreatment that consisted of two consecutive steps, the first with dilute sulfuric acid (1 % (v/v) H2SO4), and the second with alkaline solutions with different concentrations of NaOH (1, 2, 3 and 4 % (w/v)). Thermal Analysis (TG/ DTG and DTA) was used to evaluate hemicellulose, cellulose and lignin contents in the samples. Scanning Electron Microscopy (SEM) was used to evaluate the morphological structures of the in natura and chemically treated samples. Results showed that pretreatments were effective in chemical degradation of lignocellulosic materials of the samples, and also was possible to observe the morphological changes occurring in the biomasses after pretreatments.

Fuzzy Logic Based Coordinated Voltage Control for Distribution Network with Distributed Generations

This paper discusses the implementation of a fuzzy logic based coordinated voltage control for a distribution system connected with distributed generations (DGs). The connection of DGs has created a challenge for the distribution network operators to keep the voltage in the system within its acceptable limits. Intelligent centralized or coordinated voltage control schemes have proven to be more reliable due to its ability to provide more control and coordination with the communication with other network devices. In this work, voltage control using fuzzy logic by coordinating three methods of control, power factor control, on load tap changer and generation curtailment is implemented on a distribution network test system. The results show that the fuzzy logic based coordination is able to keep the voltage within its allowable limits. 

Pushing the Limits of Address Based Authentication: How to Avoid MAC Address Spoofing in Wireless LANs

It is well-known that in wireless local area networks, authenticating nodes by their MAC addresses is not secure since it is very easy for an attacker to learn one of the authorized addresses and change his MAC address accordingly. In this paper, in order to prevent MAC address spoofing attacks, we propose to use dynamically changing MAC addresses and make each address usable for only one session. The scheme we propose does not require any change in 802.11 protocols and incurs only a small performance overhead. One of the nice features of our new scheme is that no third party can link different communication sessions of the same user by monitoring MAC addresses therefore our scheme is preferable also with respect to user privacy.

Analyzing of Temperature-Dependent Thermal Conductivity Effect in the Numerical Modeling of Fin-Tube Radiators: Introduction of a New Method

In all industries which are related to heat, suitable thermal ranges are defined for each device to operate well. Consideration of these limits requires a thermal control unit beside the main system. The Satellite Thermal Control Unit exploits from different methods and facilities individually or mixed. For enhancing heat transfer between primary surface and the environment, utilization of radiating extended surfaces are common. Especially for large temperature differences; variable thermal conductivity has a strong effect on performance of such a surface .In most literatures, thermo-physical properties, such as thermal conductivity, are assumed as constant. However, in some recent researches the variation of these parameters is considered. This may be helpful for the evaluation of fin-s temperature distribution in relatively large temperature differences. A new method is introduced to evaluate temperature-dependent thermal conductivity values. The finite volume method is employed to simulate numerically the temperature distribution in a space radiating fin. The present modeling is carried out for Aluminum as fin material and compared with previous method. The present results are also compared with those of two other analytical methods and good agreement is shown.

An Archetype to Sustain Knowledge Management Systems through Intranet

Creation and maintenance of knowledge management systems has been recognized as an important research area. Consecutively lack of accurate results from knowledge management systems limits the organization to apply their knowledge management processes. This leads to a failure in getting the right information to the right people at the right time thus followed by a deficiency in decision making processes. An Intranet offers a powerful tool for communication and collaboration, presenting data and information, and the means that creates and shares knowledge, all in one easily accessible place. This paper proposes an archetype describing how a knowledge management system, with the support of intranet capabilities, could very much increase the accuracy of capturing, storing and retrieving knowledge based processes thereby increasing the efficiency of the system. This system will expect a critical mass of usage, by the users, for intranet to function as knowledge management systems. This prototype would lead to a design of an application that would impose creation and maintenance of an effective knowledge management system through intranet. The aim of this paper is to introduce an effective system to handle capture, store and distribute knowledge management in a form that may not lead to any failure which exists in most of the systems. The methodology used in the system would require all the employees, in the organization, to contribute the maximum to deliver the system to a successful arena. The system is still in its initial mode and thereby the authors are under the process to practically implement the ideas, as mentioned in the system, to produce satisfactory results.

Bioinformatic Analysis of Retroelement-Associated Sequences in Human and Mouse Promoters

Mammalian genomes contain large number of retroelements (SINEs, LINEs and LTRs) which could affect expression of protein coding genes through associated transcription factor binding sites (TFBS). Activity of the retroelement-associated TFBS in many genes is confirmed experimentally but their global functional impact remains unclear. Human SINEs (Alu repeats) and mouse SINEs (B1 and B2 repeats) are known to be clustered in GCrich gene rich genome segments consistent with the view that they can contribute to regulation of gene expression. We have shown earlier that Alu are involved in formation of cis-regulatory modules (clusters of TFBS) in human promoters, and other authors reported that Alu located near promoter CpG islands have an increased frequency of CpG dinucleotides suggesting that these Alu are undermethylated. Human Alu and mouse B1/B2 elements have an internal bipartite promoter for RNA polymerase III containing conserved sequence motif called B-box which can bind basal transcription complex TFIIIC. It has been recently shown that TFIIIC binding to B-box leads to formation of a boundary which limits spread of repressive chromatin modifications in S. pombe. SINEassociated B-boxes may have similar function but conservation of TFIIIC binding sites in SINEs located near mammalian promoters has not been studied earlier. Here we analysed abundance and distribution of retroelements (SINEs, LINEs and LTRs) in annotated sequences of the Database of mammalian transcription start sites (DBTSS). Fractions of SINEs in human and mouse promoters are slightly lower than in all genome but >40% of human and mouse promoters contain Alu or B1/B2 elements within -1000 to +200 bp interval relative to transcription start site (TSS). Most of these SINEs is associated with distal segments of promoters (-1000 to -200 bp relative to TSS) indicating that their insertion at distances >200 bp upstream of TSS is tolerated during evolution. Distribution of SINEs in promoters correlates negatively with the distribution of CpG sequences. Using analysis of abundance of 12-mer motifs from the B1 and Alu consensus sequences in genome and DBTSS it has been confirmed that some subsegments of Alu and B1 elements are poorly conserved which depends in part on the presence of CpG dinucleotides. One of these CpG-containing subsegments in B1 elements overlaps with SINE-associated B-box and it shows better conservation in DBTSS compared to genomic sequences. It has been also studied conservation in DBTSS and genome of the B-box containing segments of old (AluJ, AluS) and young (AluY) Alu repeats and found that CpG sequence of the B-box of old Alu is better conserved in DBTSS than in genome. This indicates that Bbox- associated CpGs in promoters are better protected from methylation and mutation than B-box-associated CpGs in genomic SINEs. These results are consistent with the view that potential TFIIIC binding motifs in SINEs associated with human and mouse promoters may be functionally important. These motifs may protect promoters from repressive histone modifications which spread from adjacent sequences. This can potentially explain well known clustering of SINEs in GC-rich gene rich genome compartments and existence of unmethylated CpG islands.

New Simultaneous High Performance Liquid Chromatographic Method for Determination of NSAIDs and Opioid Analgesics in Advanced Drug Delivery Systems and Human Plasma

A new and cost effective RP-HPLC method was developed and validated for simultaneous analysis of non steroidal anti inflammatory dugs Diclofenac sodium (DFS), Flurbiprofen (FLP) and an opioid analgesic Tramadol (TMD) in advanced drug delivery systems (Liposome and Microcapsules), marketed brands and human plasma. Isocratic system was employed for the flow of mobile phase consisting of 10 mM sodium dihydrogen phosphate buffer and acetonitrile in molar ratio of 67: 33 with adjusted pH of 3.2. The stationary phase was hypersil ODS column (C18, 250×4.6 mm i.d., 5 μm) with controlled temperature of 30 C°. DFS in liposomes, microcapsules and marketed drug products was determined in range of 99.76-99.84%. FLP and TMD in microcapsules and brands formulation were 99.78 - 99.94 % and 99.80 - 99.82 %, respectively. Single step liquid-liquid extraction procedure using combination of acetonitrile and trichloroacetic acid (TCA) as protein precipitating agent was employed. The detection limits (at S/N ratio 3) of quality control solutions and plasma samples were 10, 20, and 20 ng/ml for DFS, FLP and TMD, respectively. The Assay was acceptable in linear dynamic range. All other validation parameters were found in limits of FDA and ICH method validation guidelines. The proposed method is sensitive, accurate and precise and could be applicable for routine analysis in pharmaceutical industry as well as in human plasma samples for bioequivalence and pharmacokinetics studies.

Development of an Efficient CVT using Electromecanical System

Continuously variable transmission (CVT) is a type of automatic transmission that can change the gear ratio to any arbitrary setting within the limits. The most common type of CVT operates on a pulley system that allows an infinite variability between highest and lowest gears with no discrete steps. However, the current CVT system with hydraulic actuation method suffers from the power loss. It needs continuous force for the pulley to clamp the belt and hold the torque resulting in large amount of energy consumption. This study focused on the development of an electromechanical actuated control CVT to eliminate the problem that faced by the existing CVT. It is conducted with several steps; computing and selecting the appropriate sizing for stroke length, lead screw system and etc. From the visual observation it was found that the CVT system of this research is satisfactory.

Interconnection of Autonomous PROFIBUS Segments through IEEE 802.16 WMAN

PROFIBUS (PROcess FIeld BUS) which is defined with international standarts (IEC61158, EN50170) is the most popular fieldbus, and provides a communication between industrial applications which are located in different control environment and location in manufacturing, process and building automation. Its communication speed is from 9.6 Kbps to 12 Mbps over distances from 100 to 1200 meters, and so it is to be often necessary to interconnect them in order to break these limits. Unfortunately this interconnection raises several issues and the solutions found so far are not very satisfactory. In this paper, we propose a new solution to interconnect PROFIBUS segments, which uses a wireless MAN based on the IEEE 802.16 standard as a backbone system. Also, the solution which is described a model for internetworking unit integrates the traffic generated by PROFIBUS segments into IEEE 802.16 wireless MAN using encapsulation technique.

Towards an Enhanced Stochastic Simulation Model for Risk Analysis in Highway Construction

Over the years, there is a growing trend towards quality-based specifications in highway construction. In many Quality Control/Quality Assurance (QC/QA) specifications, the contractor is primarily responsible for quality control of the process, whereas the highway agency is responsible for testing the acceptance of the product. A cooperative investigation was conducted in Illinois over several years to develop a prototype End-Result Specification (ERS) for asphalt pavement construction. The final characteristics of the product are stipulated in the ERS and the contractor is given considerable freedom in achieving those characteristics. The risk for the contractor or agency depends on how the acceptance limits and processes are specified. Stochastic simulation models are very useful in estimating and analyzing payment risk in ERS systems and these form an integral part of the Illinois-s prototype ERS system. This paper describes the development of an innovative methodology to estimate the variability components in in-situ density, air voids and asphalt content data from ERS projects. The information gained from this would be crucial in simulating these ERS projects for estimation and analysis of payment risks associated with asphalt pavement construction. However, these methods require at least two parties to conduct tests on all the split samples obtained according to the sampling scheme prescribed in present ERS implemented in Illinois.