Abstract: An optimum technic has been developed for extracting volatile organic compounds which contribute to the aroma of lime fruit (Citrus aurantifolia). The volatile organic compounds of healthy and infested lime fruit with California red scale Aonidiella aurantii were characterized using headspace solid phase microextraction (HS-SPME) combined with gas chromatography (GC) coupled flame ionization detection (FID) and gas chromatography with mass spectrometry (GC-MS) as a very simple, efficient and nondestructive extraction method. A three-phase 50/30 μm PDV/DVB/CAR fibre was used for the extraction process. The optimal sealing and fibre exposure time for volatiles reaching equilibrium from whole lime fruit in the headspace of the chamber was 16 and 4 hours respectively. 5 min was selected as desorption time of the three-phase fibre. Herbivorous activity induces indirect plant defenses, as the emission of herbivorous-induced plant volatiles (HIPVs), which could be used by natural enemies for host location. GC-MS analysis showed qualitative differences among volatiles emitted by infested and healthy lime fruit. The GC-MS analysis allowed the initial identification of 18 compounds, with similarities higher than 85%, in accordance with the NIST mass spectral library. One of these were increased by A. aurantii infestation, D-limonene, and three were decreased, Undecane, α-Farnesene and 7-epi-α-selinene. From an applied point of view, the application of the above-mentioned VOCs may help boost the efficiency of biocontrol programs and natural enemies’ production techniques.
Abstract: The squid Loligo forbesii is suspected to be an important species in marine food webs, as it can strongly impact its prey and be impacted upon by predation, competition, fishing and/or climate variability. To quantify these impacts in the food web, the measurement of its trophic position and ecological role within well-studied ecosystems is essential. An Ecopath model was balanced and run for the Moray Firth ecosystem and was used to investigate the significance of this squid’s trophic roles. The network analysis routine included in Ecopath with Ecosim (EwE) was used to estimate trophic interaction, system indicators (health condition and developmental stage) and food web features. Results indicated that within the Moray Firth squid occupy a top trophic position in the food web and also a major prey item for many other species. Results from Omnivory Index (OI) showed that squid is a generalized feeder transferring energy across wide trophic levels and is more important as a predator than that as a prey in the Moray Firth ecosystem. The results highlight the importance of taking squid into account in the management of Europe’s living marine resources.
Abstract: A suitable model membrane to study the pharmacological effect of pharmaceutical products is human stratum corneum because this layer of human skin is the outermost layer and it is an important barrier to be passed through. Other model membranes which were also used are for example skins from pig, mouse, reptile or fish. We are interested in fish skins in this project. The advantages of the fish skins are, that they can be obtained from the supermarket or fish shop. However, the fish skins should be freshly prepared and used directly without storage. In order to understand the effect of different model drugs e.g. lidocaine HCl, resveratrol, paracetamol, ibuprofen, acetyl salicylic acid on the properties of the model membrane from various types of fishes e.g. trout, salmon, cod, plaice permeation tests were performed and differential scanning calorimetry was applied.
Abstract: The effect of calcium salts on the storage stability and on the quality attributes of both fresh and processed product (guava powder) of white flesh guavas (var ‘Allahabad safeda’) was studied. The pulp behavioral studies of fully ripened guava fruits indicated that fruits pretreated with 3% and 4.5% calcium chloride had the least viscosity. The guava pulp powder using spray drying technique was developed and its storage stability and the moisture sorption studies were carried out for product quality evaluation at normal storage condition (27°C; 65%RH). Results revealed that powder obtained from 3% calcium chloride pretreated guavas was found to be at par with the powder obtained from control guavas after 90 days of normal storage. Studies on microbiological quality of guava pulp powder indicated that among the treatments powder obtained from guava fruit pretreated with 3% calcium chloride to be the most effective through restricting microbial counts of total plate count, yeast, mold, Staphylococcus and E. coli below their permissible limit. Moisture sorption studies of guava powder revealed that foil laminate 12μm PET/9 μm foil/38-40 μm is the most suitable packaging material recommended.
Abstract: In this study, total fatty acid composition of muscle
lipids of Cyprinus carpio L. living in Suğla Dam Lake, Altinapa Dam
Lake, Eğirdir Lake and Burdur Lake were determined using GC.
During this study, for the summer season of July was taken from each
region of the land and they were stored in deep-freeze set to -20
degrees until the analysis date. At the end of the analyses, 30
different fatty acids were found in the composition of Cyprinus
carpio L. which lives in different lakes. Cyprinus carpio Suğla Dam
Lake of polyunsaturated fatty acids (PUFAs), were higher than other
lakes. Cyprinus carpio L. was the highest in the major SFA palmitic
acid. Polyunsaturated fatty acids (PUFA) of carp, the most abundant
fish species in all lakes, were found to be higher than those of
saturated fatty acids (SFA) in all lakes. Palmitic acid was the major
SFA in all lakes. Oleic acid was identified as the major MUFA.
Docosahexaenoic acid (DHA) was the most abundant in all lakes. ω3
fatty acid composition was higher than the percentage of the
percentage ω6 fatty acids in all lake. ω3/ω6 rates of Cyprinus carpio
L. Suğla Dam Lake, Altinapa Dam Lake, Eğirdir Lake and Burdur
Lake, 2.12, 1.19, 2.15, 2.87, and 2.82, respectively. Docosahexaenoic
acid (DHA) was the major PUFA in Eğirdir and Burdur lakes,
whereas linoleic acid (LA) was the major PUFA in Altinapa and
Suğla Dam Lakes. It was shown that the fatty acid composition in the
muscle of carp was significantly influenced by different lakes.
Abstract: Red pepper (Capsicum annum L.) has long been
recognized as a good source of antioxidants, being rich in ascorbic
acid and other phytochemicals. In Turkish cuisine red pepper is
sometimes consumed raw in salads and baked as a garnish, but its
most wide consumption type is red pepper paste. The processing of
red pepper into pepper paste includes various thermal treatment steps
such as heating and pasteurizing. There are reports demonstrating an
enhancement or reduction in antioxidant activity of vegetables after
thermal treatment. So this study was conducted to investigate the
total phenolic, ascorbic acid and total carotenoids as well as free
radical scavenging activity of raw red pepper and various red pepper
pastes obtainable on the market. The samples were analyzed for
radical-scavenging activity (RSA) and total polyphenol (TP) content
using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Folin-Ciocalteu
methods, respectively. Total carotenoids and ascorbic acid contents
were determined spectrophotometrically. Results suggest that hot
pepper paste contained significantly (P0.05) difference in RSA, ascorbic acid and total carotenoids
content between sweet and hot red pepper paste products. It is
concluded that the red pepper paste, that has a wide range of
consumption in Turkish cuisine, presents a good dose of phenolic
compounds and antioxidant capacity and it should be regarded as a
functional food.
Abstract: The present explanatory study concerns with the
relation between Diabetes Mellitus and Food Balance in the Kingdom
of Saudi Arabia during 2005-2010, using published data. Results illustrated that Saudi citizen daily protein consumption
(DPC) during 2005-2007 (g/capita/day) is higher than the average
global consumption level of protein with 15.27%, daily fat
consumption (DFC) with 24.56% and daily energy consumption
(DEC) with 16.93% and increases than recommended level by
International Nutrition Organizations (INO) with 56% for protein,
60.49% for fat and 27.37% for energy. On the other hand, DPC per
capita in Saudi Arabia decreased during the period 2008-2010 from
88.3 to 82.36 gram/ day. Moreover, DFC per capita in Saudi Arabia
decreased during the period 2008-2010 from 3247.90 to 3176.43
Cal/capita/ day, and daily energy consumption (DEC) of Saudi
citizen increases than world consumption with 16.93%, whereas
increases with 27.37% than INO. Despite this, DPC, DFC and DEC
per capita in Saudi Arabia still higher than world mean. On the other
side, results illustrated that the number of diabetic patients in Saudi
Arabia during the same period (2005-2010). The curve of diabetic
patient’s number in Saudi Arabia during 2005-2010 is regular
ascending with increasing level ranged between 7.10% in 2005 and
12.44% in 2010. It is essential to devise Saudi National programs to educate the
public about the relation of food balances and diabetes so it could be
avoided, and provide citizens with healthy dietary balances tables.
Abstract: Objectives: To determine the nutritional status and
risk factors associated with women practicing geophagia in QwaQwa,
South Africa. Materials and Methods: An observational epidemiological study
design was adopted which included an exposed (geophagia) and nonexposed
(control) group. A food frequency questionnaire, anthropometric measurements and blood sampling were applied to
determine nutritional status of participants. Logistic regression
analysis was performed in order to identify factors that were likely to
be associated with the practice of geophagia. Results: The mean total energy intake for the geophagia group (G)
and control group (C) were 10324.31 ± 2755.00 kJ and 10763.94 ±
2556.30 kJ respectively. Both groups fell within the overweight
category according to the mean Body Mass Index (BMI) of each
group (G= 25.59 kg/m2; C= 25.14 kg/m2). The mean serum iron
levels of the geophagia group (6.929 μmol/l) were significantly lower
than that of the control group (13.75 μmol/l) (p = 0.000). Serum
transferrin (G=3.23g/l; C=2.7054g/l) and serum transferrin saturation
(G=8.05%; C=18.74%) levels also differed significantly between
groups (p=0.00). Factors that were associated with the practice of
geophagia included haemoglobin (Odds ratio (OR):14.50), serumiron
(OR: 9.80), serum-ferritin (OR: 3.75), serum-transferrin (OR:
6.92) and transferrin saturation (OR: 14.50). A significant negative
association (p=0.014) was found between women who were wageearners
and those who were not wage-earners and the practice of
geophagia (OR: 0.143; CI: 0.027; 0.755). These findings seem to
indicate that a permanent income may decrease the likelihood of
practising geophagia. Key Findings: Geophagia was confirmed to be a risk factor for
iron deficiency in this community. The significantly strong
association between geophagia and iron deficiency emphasizes the
importance of identifying the practice of geophagia in women,
especially during their child bearing years.
Abstract: Tannins are a unique category of plant phytochemicals
especially in terms of their vast potential health-benefiting properties.
Researchers have described the capacity of tannins to enhance
glucose uptake and inhibit adipogenesis, thus being potential drugs
for the treatment of non-insulin dependent diabetes mellitus. Thus,
the present research was conducted to find out tannin content of food
products. The percentage of tannin in various analyzed sources
ranged from 0.0 to 108.53%; highest in kathaa and lowest in ker and
mango bark. The percentage of tannins present in the plants,
however, varies. Numerous studies have confirmed that the naturally
occurring polyphenols are key factor for the beneficial effects of the
herbal medicines. Isolation and identification of active constituents
from plants, preparation of standardized dose & dosage regimen can
play a significant role in improving the hypoglycaemic action.
Abstract: The consumption of food contaminated with molds
(microscopic filamentous fungi) and their toxic metabolites results in
the development of food-borne mycotoxicosis. The spores of molds
are ubiquitously spread in the environment and can be detected
everywhere. Ochratoxin A is a toxic and potentially carcinogenic
fungal toxin found in a variety of food commodities. In this study, the
mycological quality of various ready-to-eat local and imported pork
meat and meat byproducts sold in Egyptian markets were assessed
and the presence of various molds was determined in pork used as a
raw material, edible organs as liver and kidney as well as in
fermented raw meat by-products. The study assessed the mycological
quality of pork raw meat and their by-products sold in commercial
shops in Cairo, Egypt. Mycological analysis was conducted on
(n=110) samples which included pig’s livers and kidneys from
Egyptian Bassatin slaughter house; local and imported processed
pork meat by-products from Egyptian pork markets. The isolates
were identified using traditional mycological and biochemical tests.
All kidney and liver samples were positive to molds growth while all
byproducts were negative. Ochratoxin A levels were quantitatively
analyzed using the high performance liquid chromatography (HPLC)
and the highest results were present in kidney 7.51 part per billion
(ppb) followed by minced meat 6.19 ppb generally the local samples
showed higher levels than the imported ones. To the best of our
knowledge, this is the first report on mycotoxins detection and
quantification from pork by-products in Egypt.
Abstract: This research was planned in order to study the effect
of drought stress and different levels of Superabsorbent and their
effect on grain yield, biologic yield and harvest index. In this study, 3
different depths of irrigation were considered as the main treatment
I1, I2, I3 as 100, 75 and 50 percent of water requirement of plants
respectively and different levels of Superabsorbent were used as
secondary treatment (S0, S1, S2 and S3, equal to 0 (control), 15, 30
and 45 gr/m2 respectively). According to the results, independent
effects of irrigation and Superabsorbent treatments at 1% level on
biologic and grain yield of corn were significant. In addition,
independent effect of irrigation treatments at 5% level on harvest
index was significant. But independent effect of Superabsorbent
treatments on harvest index was not significant.
Abstract: Electrostatic interaction energy (ΔEEDL) is a part of the Extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory, which, together with van der Waals (ΔEVDW) and acid base (ΔEAB) interaction energies, has been extensively used to investigate the initial adhesion of bacteria to surfaces. Electrostatic or electrical double layer interaction energy is considerably affected by surface potential; however it cannot be determined experimentally and is usually replaced by zeta (ζ) potential via electrophoretic mobility. This paper focusses on the effect of ionic concentration as a function of pH and the effect of mineral grain size on ζ potential. It was found that both ionic strength and mineral grain size play a major role in determining the value of ζ potential for the adhesion of P. putida to hematite and quartz surfaces. Higher ζ potential values lead to higher electrostatic interaction energies and eventually to higher total XDLVO interaction energy resulting in bacterial repulsion.
Abstract: Consumers are demanding novel beverages that are
healthier, convenient and have appealing consumer acceptance. The
objectives of this study were to investigate the effects of adding grape
polyphenols and the influence of presenting health claims on the
sensory acceptability of wines. Fresh red sorrel calyces were
fermented into wines. The total soluble solids of the pectinase-treated
sorrel puree were from 4°Brix to 23.8°Brix. Polyphenol in the form
of grape pomace extract was added to sorrel wines (w/v) in specified
levels to give 0. 25. 50 and 75 ppm. A focus group comprising of 12
panelists was use to select the level of polyphenol to be added to
sorrel wines for sensory preference The sensory attributed of the
wines which were evaluated were colour, clarity, aroma, flavor,
mouth-feel, sweetness, astringency and overall preference. The sorrel
wine which was most preferred from focus group evaluation was
presented for hedonic rating. In the first stage of hedonic testing, the
sorrel wine was served chilled at 7°C for 24 h prior to sensory
evaluation. Each panelist was provided with a questionnaire and was
asked to rate the wines on colour, aroma, flavor, mouth-feel,
sweetness, astringency and overall acceptability using a 9-point
hedonic scale. In the second stage of hedonic testing, the panelist
were instructed to read a health abstract on the health benefits of
polyphenolic compounds and again to rate sorrel wine with added 25
ppm polyphenol. Paired t-test was used for the analysis of the
influence of presenting health information on polyphenols on hedonic
scoring of sorrel wines. Focus groups found that the addition of
polyphenol addition had no significant effect on sensory color and
aroma but affected clarity and flavor. A 25 ppm wine was liked
moderately in overall acceptability. The presentation of information
on the health benefit of polyphenols in sorrel wines to panelists had
no significant influence on the sensory acceptance of wine. More
than half of panelists would drink this wine now and then. This wine
had color L 19.86±0.68, chroma 2.10±0.12, hue° 16.90 ±3.10 and
alcohol content of 13.0%. The sorrel wine was liked moderately in
overall acceptability with the added polyphenols.
Abstract: Effects of nicotine on pre-partum body weight and
preimplantation embryonic development has been reported
previously. Present study was conducted to determine the effects of
annatto (Bixa orellana)-derived delta-tocotrienol (TCT) (with
presence of 10% gamma-TCT isomer) on the nicotine-induced
reduction in body weight and 8-cell embryonic growth in mice.
Twenty-four 6-8 weeks old (23-25g) female balb/c mice were
randomly divided into four groups (G1-G4; n=6). Those groups were
subjected to the following treatments for 7 consecutive days: G1
(control) were gavaged with 0.1 ml tocopherol stripped corn oil. G2
was subcutaneously (s.c.) injected with 3 mg/kg/day of nicotine. G3
received concurrent treatment of nicotine (3 mg/kg/day) and 60
mg/kg/day of δ-TCT mixture (contains 90% delta & 10% gamma
isomers) and G4 was given 60 mg/kg/day of δ-TCT mixture alone.
Body weights were recorded daily during the treatment. On Day 8,
females were superovulated with 5 IU Pregnant Mare’s Serum
Gonadotropin (PMSG) for 48 hours followed with 5 IU human
Chorionic Gonadotropin (hCG) before mated with males at the ratio
of 1:1. Females were sacrificed by cervical dislocation for embryo
collection 48 hours post-coitum. Collected embryos were cultured in
vitro. Results showed that throughout Day 1 to Day 7, the body
weight of nicotine treated group (G2) was significantly lower
(p
Abstract: Grains, including oats (Avena sativa L.), have been
recognized functional foods, because provide beneficial effect on the
health of the consumer and decrease the risk of various diseases. Oats
are good source of soluble fibre, essential amino acids, unsaturated
fatty acids, vitamins and minerals. Oat breeders have developed oat
varieties and improved yielding ability potential of oat varieties.
Therefore, the aim of investigation was to analyze the composition of
perspective oat varieties and breeding lines grains grown in different
conditions and evaluate functional properties. In the studied samples
content of protein, starch, β-glucans, total dietetic fibre, composition
of amino acids and vitamin E were determined. The results of
analysis showed that protein content depending of varieties ranged
9.70% to 17.30% total dietary fibre 13.66 g100g-1 to 30.17 g100g-1,
content of β-glucans 2.7 g100g-1 to 3.5 g100g-1, amount of
vitamin E (α-tocopherol) determined from 4 mgkg-1 to 9.9 mgkg-1.
The sums of essential amino acids in oat grain samples were
determined from 31.63 gkg-1 to 54.90 gkg-1. It is concluded that
amino acids composition of husked and naked oats grown in organic
or conventional conditions is close to optimal for human health.
Abstract: Several embryonic cellular mechanism including cell
cycle, growth and apoptosis are regulated by phosphatidylinositol-3-
kinase (PI3K)/Akt signaling pathway. The goal of present study is to
determine the effects of annatto (Bixa orellana)-derived δ-tocotrienol
(δ-TCT) on the regulations of PI3K/Akt genes in murine morula.
Twenty four 6-8 week old (23-25g) female balb/c mice were
randomly divided into four groups (G1-G4; n=6). Those groups were
subjected to the following treatments for 7 consecutive days: G1
(control) received tocopherol stripped corn oil, G2 was given 60
mg/kg/day of δ-TCT mixture (contains 90% delta & 10% gamma
isomers), G3 was given 60 mg/kg/day of pure δ-TCT (>98% purity)
and G4 received 60 mg/kg/day α-TOC. On Day 8, females were
superovulated with 5 IU Pregnant Mare’s Serum Gonadotropin
(PMSG) for 48 hours followed with 5 IU human Chorionic
Gonadotropin (hCG) before mated with males at the ratio of 1:1.
Females were sacrificed by cervical dislocation for embryo collection
48 hours post-coitum. About fifty morulas from each group were
used in the gene expression analyses using Affymetrix QuantiGene
Plex 2.0 Assay. Present data showed a significant increase (p
Abstract: In this study, lipid-deprived residuals of microalgae
were hydrolyzed for the production of reducing sugars by using the
recombinant Bacillus cellulosome, carrying eight genes from the
Clostridium thermocellum ATCC27405. The obtained cellulosome
was found to exist mostly in the broth supernatant with a cellulosome
activity of 2.4 U/mL. Furthermore, the Michaelis-Menten constant
(Km) and Vmax of cellulosome were found to be 14.832 g/L and 3.522
U/mL. The activation energy of the cellulosome to hydrolyze
microalgae LDRs was calculated as 32.804 kJ/mol.
Abstract: SARA is a common and serious metabolic disorder in
early lactation in dairy cattle and in finishing beef cattle, caused by
diets with high inclusion of cereal grain. This experiment was
performed to determine the efficacy of Megasphaera elsdenii, a
major lactate-utilizing bacterium in prevention/treatment of SARA in
vivo. In vivo experimentation, it was used eight ruminally cannulated
rams and it was applied the rapid adaptation with the mixture of grain
based on wheat (80% wheat, 20% barley) and barley (80% barley,
20% wheat). During the systematic adaptation, it was followed the
probability of SARA formation by being measured the rumen pH
with two hours intervals after and before feeding. After being
evaluated the data, it was determined the ruminal pH ranged from
5.2-5.6 on the condition of feeding with 60 percentage of grain
mixture based on barley and wheat, that assured the definite form of
subacute acidosis. In four days SARA period, M. elsdenii (1010 cfu
ml-1) was inoculated during the first two days. During the SARA
period, it was observed the decrease of feed intake with M. elsdenii
inoculation. Inoculation of M. elsdenii was caused to differentiation
of rumen pH (P
Abstract: Psyllium gum alone and in combination with
sunflower oil was investigated as a possible alternative edible coating
for improvement of quality and shelf life of fresh-cut papaya.
Different concentrations including 0.5, 1 and 1.5 percent of psyllium
gum were used for coating of fresh-cut papaya. In some samples,
refined sunflower oil was used as a lipid component to increase the
effectiveness of coating in terms of water barrier properties. Soya
lecithin was used as an emulsifier in coatings containing oil.
Pretreatment with 1% calcium chloride was given to maintain the
firmness of fresh-cut papaya cubes. 1% psyllium gum coating was
found to yield better results. Further, addition of oil helped to
maintain the quality and acted as a barrier to water vapour, therefore,
minimizing the weight loss.
Abstract: We used live E. coli containing synthetic genetic
oscillators to study how the degree of synchrony between the genetic
circuits of sister cells changes with temperature. We found that both
the mean and the variability of the degree of synchrony between the
fluorescence signals from sister cells are affected by temperature.
Also, while most pairs of sister cells were found to be highly
synchronous in each condition, the number of asynchronous pairs
increased with increasing temperature, which was found to be due to
disruptions in the oscillations. Finally we provide evidence that these
disruptions tend to affect multiple generations as opposed to
individual cells. These findings provide insight in how to design
more robust synthetic circuits and in how cell division can affect their
dynamics.