Abstract: Land with low pH soil spread widely in Indonesia
can be used for soybean (Glycine max) cultivation, however the
production is low. The use of acid tolerant soybean and acidaluminium
tolerant nitrogen-fixing bacteria formula was an
alternative way to increase soybean productivity on acid soils.
Bradyrhizobium japonicum is one of the nitrogen fixing bacteria
which can symbiose with soybean plants through root nodule
formation. Most of the nitrogen source required by soybean plants
can be provided by this symbiosis. This research was conducted to
study the influence of acid-aluminium tolerant B. japonicum strain
BJ 11 formula using peat as carrier on growth of Tanggamus and
Anjasmoro cultivar soybean planted on acid soil fields (pH 5.0-
5.5). The results showed that the inoculant was able to increase the
growth and production of soybean which were grown on fields acid
soil at Sukadana (Lampung) and Tanah Laut (South Kalimantan),
Indonesia.
Abstract: The objective of this research was to determine the
potency of indigenous acid-aluminium tolerant Bradyrhizobium
japonicum as producer of indole acetic acid (IAA) and applied it as
nitrogen fixation on local soybeans viz Anjasmoro, Tanggamus
(yellow soybean seeds), and Detam (black soybean seed). Three
isolates of acid-aluminium tolerant Bradyrhizobium japonicum (BJ)
were used in this research, i.e. BJ 11 (wt), BJ 11 (19) - BJ 11(wt)
mutant, and USDA 110 as a reference isolate. All of isolates tested to
produce the IAA by using Salkowsky method. Effect of IAA
production by each of B. japonicum was tested on growth pouch and
greenhouse using three varieties of soybean. All isolates could grow
well and produce IAA on yeast mannitol broth (YMB) medium in
the presence of 0.5 mM L-tryptophan. BJ 11 (19) produced the
highest of IAA at 4 days incubation compared to BJ 11 (wt) and
USDA 110. All tested isolates of Bradyrhizobium japonicum have
showed effect on stimulating the formation of root nodules in
soybean varieties grown on Leonard bottle. The concentration of
IAA on root nodules of soybean symbiotic with B. japonicum was
significantly different with control, except on the treatment using
Tanggamus soybean.