The Use of Acid-Aluminium Tolerant Bradyrhizobium japonicum Formula for

Land with low pH soil spread widely in Indonesia can be used for soybean (Glycine max) cultivation, however the production is low. The use of acid tolerant soybean and acidaluminium tolerant nitrogen-fixing bacteria formula was an alternative way to increase soybean productivity on acid soils. Bradyrhizobium japonicum is one of the nitrogen fixing bacteria which can symbiose with soybean plants through root nodule formation. Most of the nitrogen source required by soybean plants can be provided by this symbiosis. This research was conducted to study the influence of acid-aluminium tolerant B. japonicum strain BJ 11 formula using peat as carrier on growth of Tanggamus and Anjasmoro cultivar soybean planted on acid soil fields (pH 5.0- 5.5). The results showed that the inoculant was able to increase the growth and production of soybean which were grown on fields acid soil at Sukadana (Lampung) and Tanah Laut (South Kalimantan), Indonesia.

Authors:

• Nisa Rachmania Mubarik
• Tedja Imas
• Aris Tri Wahyudi
• Triadiati
• Suharyanto
• Happy Widiastuti

Keywords:

• Bradyrhizobium japonicum
• acid-aluminium tolerant mutant
• Tanggamus cultivar soybean
• acid soils

References:

[1] P. Somasegaran and H.J. Hoben. Methods in legume-Rhizobium
Technology. Paia: University of Hawaii , 1994.
[2] M.K. Abbasi, A. Majeed, A.Sadiq, and S.R. Khan.. Application of
Bradyrhizobium japonicum and phosphorus fertilization improved
growth, yields and nodulation of soybean in the sub-humid hilly region
of Azad Jammu and Khasmir, Pakistan. Plant Prod. Sci., vol. 11, pp.
368-376, 2008.
[3] A. Ayanaba, S. Asanuma, and D.N. Munns, "An agar plate method for
rapid screening of Rhizobium tolerance to acid aluminium stress," Soil.
Sci. Am. J ., vol. 47, pp. 256-258, 1983.
[4] R.M. Atlas and R. Bartha. Microbial Ecology, Fundamentals and
Application. 4th Ed. Menlo Park: Addison Wesley Longman, Inc,
1998.
[5] J. Denarie, P. Debelle, and C. Rosenberg, "Signaling and host range
variation in nodulation, " Ann. Rev. Microbiol.,vol. 46, pp.497-531,
1992.
[6] T. Endarini, A.T. Wahyudi, Tedja-Imas, "Selection of indigenous
Bradyrhizobium japonicum tolerant acid-aluminium medium" (in
Indonesia language), Hayati, vol. 2, pp. 74-79, 1995.
[7] H. Habibah. Effectivity of acid-aluminium tolerant Bradyrhizobium
japonicum on the growth of Slamet [thesis, in Indonesia language].
Bogor: Postgraduate School, Bogor Agricultural University, 2008.
[8] A.R.F. Situmorang, N.R. Mubarik, and Triadiati, "The use of inoculant
acid- aluminium tolerant Bradyrhizobium japonicum for soybean
growth on acid soils," Hayati J. Biosci., vol. 16, pp. 157-160, 2009.
[9] A.T. Wahyudi, A. Suwanto, Tedja-Imas and A. Tjahyoleksono,
"Screening of acid-aluminium tolerant Bradyrhizobium japonicum strain
analysis of marker genes and competition in planta," Aspac. J. Mol. Biol.
Biotechnol . vol. 6, pp. 13-20, 1998.
[10] X. Parveen, D.T. Webb, D. Borthakur, "The symbiotic phenotypes of
exopoly- saccharide defective mutants of Rhizobium sp. strain TAL1145
do not differ on determinate and indeterminate nodulating tree legume,"
Microbiology, vol. 143, pp. 1959-1967, 1997.
[11] A.H. Lounch, Karen, and J Miller. "Synthesis of low-molecular-weight
form of exopoly- saccharide by Bradyrhizobium japonicum USDA
110," Appl. Environ. Microbiol., vol. 2, pp. 1011- 1014, 2001.
[12] R. Saraswati,"Technology of multipurpose microbial fertilizer
supporting sustainable system of soybean production," J Mikrobiol
Indones, vol. 4, pp. 1-9, 1999.