Abstract: A sensitive and specific method for quantitative
determination of aflatoxins(B1, B2, G1,G2), deoxynivalenol,
fumonisin(B1,B2), ochratoxin A, zearalenone, T-2 and HT-2 in
roasted and ground grains using liquid chromatography combined
with tandem mass spectrometry. A double extraction using a
phosphate buffer solution followed by methanol was applied to
achieve effective co extraction of 11 mycotoxins. A multitoxin
immunoaffinity column for all these mycotoxins was used to clean up
the extract. The LODs of mycotoxins were 0.1~6.1 μg/kg, LOQs were
0.3~18.4 μg/kg. Forty seven samples collected from Seoul (Korea) for
mycotoxin contamination monitoring. The results showed that the
occurrence of zearalenone and deoxynivalenol were frequent.
Zearalenone was detected in all samples and deoxynivalenol was
detected in 80.9 % samples in the range 0.626 ~ 29.264 μg/kg and N.D
~ 48.332 μg/kg respectively. Fumonisins and ochratoxin A were
detected in 46.8% samples and 17 % samples respectively, aflatoxins
and T-2/HT-2 toxins were not detected all samples.