Analysis of Roasted and Ground Grains on the Seoul (Korea) Market for Their Contaminants of Aflatoxins, Ochratoxin A and Fusarium Toxins by LC-MS/MS
A sensitive and specific method for quantitative
determination of aflatoxins(B1, B2, G1,G2), deoxynivalenol,
fumonisin(B1,B2), ochratoxin A, zearalenone, T-2 and HT-2 in
roasted and ground grains using liquid chromatography combined
with tandem mass spectrometry. A double extraction using a
phosphate buffer solution followed by methanol was applied to
achieve effective co extraction of 11 mycotoxins. A multitoxin
immunoaffinity column for all these mycotoxins was used to clean up
the extract. The LODs of mycotoxins were 0.1~6.1 μg/kg, LOQs were
0.3~18.4 μg/kg. Forty seven samples collected from Seoul (Korea) for
mycotoxin contamination monitoring. The results showed that the
occurrence of zearalenone and deoxynivalenol were frequent.
Zearalenone was detected in all samples and deoxynivalenol was
detected in 80.9 % samples in the range 0.626 ~ 29.264 μg/kg and N.D
~ 48.332 μg/kg respectively. Fumonisins and ochratoxin A were
detected in 46.8% samples and 17 % samples respectively, aflatoxins
and T-2/HT-2 toxins were not detected all samples.
[1] WHO/FAO. Safety Evaluation of certain Mycotoxins in Food, WHO
Food Additives Series 47/FAO Food and Nutrition Paper 74, WHO:
Geneva, 2001;1
[2] P.Ambrosino, F. Galvano. V., Foglino, A. Logrieco, R. Fresa and A.
Ritieni, "Supercritical fluid extraction of Beauvericin from
maize",Talanta, vol. 62, 2004, pp.523-.530
[3] M.P.E.Gonzalez,J. Mattusch and R. Wennrich, "Stability and
determination of aflatoxins by high-performance liquid chromatography
with amperometric detection" J. Chromatogr,A , vol. 828, 1998, pp.
439-444
[4] L. Gonzalez-Osnaya, C.Cortes, J.M.Soriano, J.C.Molto and J.Manes,
"Occurrence of deoxynivalenol and T-2 toxin in bread and pasta
commercialized in Spain." Food chemistry, vol.124, 2011, pp.156-161.
[5] K.F.Nielsen, U.Thrane, "Fast methods for screening of trichothecenes in
fungal cultures using gas chromatography- tandem mass spectrometry"
J.Chromatogr. A, vol.929, 2001, pp75-87.
[6] F.Grippi, L.Crosta, G.Aiello, M.Tolomeo, F.Oliveri, N.Gebbia,
A.Curione "Determination of stilbenes in Sicilian pistachio by
high-performance liquid chromatographic diode array
(HPLC-DAD/FLD) and evaluation of eventually mycotoxin
contamination." Food chemistry, vol.107, 2008, pp.483-488.
[7] R.Krska, R.Schuhmacher, M.Grasserbauer, P.M.Scott, J. "Determination
of the Fusarium mycotoxin beauvericin at ╬╝ g/kg levels in corn by
high-performance liquid chromatography with diode-array detection"
J.Chromatogr. A, vol. 746, 1996, pp. 233-238
[8] Anne Molinie, Virginie Faucet, Marcel Castegnaro, Annie
Pfohl-Leszkowicz. "Analysis of some breakfast cereals on the French
market for their contents of ochratoxin A, citrinin and fumonisin B1:
development of a method for simultaneous extraction of ochratoxin A and
citrinin." Food chemistry, vol. 92, 2005, pp. 391-400
[9] B.Giancaklo, B.Elisabetta, C.Edmondo, C.Valeriana, T.Giuseppina,
"Determination of ochratoxin A in eggs and target tissues of
experimentally drugged hens using HPLC- FLD." Food chemistry, vol.
126, 2011, pp. 1278-1282
[10] S.De Baere, J.Goossens, A.Osselaere, M.Devreese, V.Vandenbroucke,
P.De Backer, S.Croubels. "Quantitative determination of T-2 toxin, HT-2
toxin, deoxynivalenol and deepoxy-deoxynivalenol in animal body fluids
using LC-MS/MS detection."J.chromatogr B vol.879, 2001, pp.
2403-2415.
[11] Z.Zou, Z.He, H.Li, P.Han, J.Tang, C.Xi, Y.Li, L.Zhang, X.Li
" Development and application of a method for the analysis of two
trichothecenes:Deoxynivalenol and T-2 toxin in meat in China by
HPLC-MS/MS" Meat science, vol. 90, 2012, pp.613-617.
[12] V. M. T. Lattanzio, M.Solfizzo, S.Powers, A.Visconti. "Simultaneous
determination of aflatoxins, ochratoxin A and Fusarium toxins in maize
by liquid chromatography/tandem mass spectrometry after multitoxin
immunoaffinity clean up." Rapid commun. Mass spectrum..vol. 21. 2007.
pp.3253-3261.
[13] International Conference on Harmonization of Technical Requirements
for registration of Pharmaceuticals for Human Use (ICH), Validation of
Analytical Procedures Methodology, Geneva, Switzerland,
November .1996.
[1] WHO/FAO. Safety Evaluation of certain Mycotoxins in Food, WHO
Food Additives Series 47/FAO Food and Nutrition Paper 74, WHO:
Geneva, 2001;1
[2] P.Ambrosino, F. Galvano. V., Foglino, A. Logrieco, R. Fresa and A.
Ritieni, "Supercritical fluid extraction of Beauvericin from
maize",Talanta, vol. 62, 2004, pp.523-.530
[3] M.P.E.Gonzalez,J. Mattusch and R. Wennrich, "Stability and
determination of aflatoxins by high-performance liquid chromatography
with amperometric detection" J. Chromatogr,A , vol. 828, 1998, pp.
439-444
[4] L. Gonzalez-Osnaya, C.Cortes, J.M.Soriano, J.C.Molto and J.Manes,
"Occurrence of deoxynivalenol and T-2 toxin in bread and pasta
commercialized in Spain." Food chemistry, vol.124, 2011, pp.156-161.
[5] K.F.Nielsen, U.Thrane, "Fast methods for screening of trichothecenes in
fungal cultures using gas chromatography- tandem mass spectrometry"
J.Chromatogr. A, vol.929, 2001, pp75-87.
[6] F.Grippi, L.Crosta, G.Aiello, M.Tolomeo, F.Oliveri, N.Gebbia,
A.Curione "Determination of stilbenes in Sicilian pistachio by
high-performance liquid chromatographic diode array
(HPLC-DAD/FLD) and evaluation of eventually mycotoxin
contamination." Food chemistry, vol.107, 2008, pp.483-488.
[7] R.Krska, R.Schuhmacher, M.Grasserbauer, P.M.Scott, J. "Determination
of the Fusarium mycotoxin beauvericin at ╬╝ g/kg levels in corn by
high-performance liquid chromatography with diode-array detection"
J.Chromatogr. A, vol. 746, 1996, pp. 233-238
[8] Anne Molinie, Virginie Faucet, Marcel Castegnaro, Annie
Pfohl-Leszkowicz. "Analysis of some breakfast cereals on the French
market for their contents of ochratoxin A, citrinin and fumonisin B1:
development of a method for simultaneous extraction of ochratoxin A and
citrinin." Food chemistry, vol. 92, 2005, pp. 391-400
[9] B.Giancaklo, B.Elisabetta, C.Edmondo, C.Valeriana, T.Giuseppina,
"Determination of ochratoxin A in eggs and target tissues of
experimentally drugged hens using HPLC- FLD." Food chemistry, vol.
126, 2011, pp. 1278-1282
[10] S.De Baere, J.Goossens, A.Osselaere, M.Devreese, V.Vandenbroucke,
P.De Backer, S.Croubels. "Quantitative determination of T-2 toxin, HT-2
toxin, deoxynivalenol and deepoxy-deoxynivalenol in animal body fluids
using LC-MS/MS detection."J.chromatogr B vol.879, 2001, pp.
2403-2415.
[11] Z.Zou, Z.He, H.Li, P.Han, J.Tang, C.Xi, Y.Li, L.Zhang, X.Li
" Development and application of a method for the analysis of two
trichothecenes:Deoxynivalenol and T-2 toxin in meat in China by
HPLC-MS/MS" Meat science, vol. 90, 2012, pp.613-617.
[12] V. M. T. Lattanzio, M.Solfizzo, S.Powers, A.Visconti. "Simultaneous
determination of aflatoxins, ochratoxin A and Fusarium toxins in maize
by liquid chromatography/tandem mass spectrometry after multitoxin
immunoaffinity clean up." Rapid commun. Mass spectrum..vol. 21. 2007.
pp.3253-3261.
[13] International Conference on Harmonization of Technical Requirements
for registration of Pharmaceuticals for Human Use (ICH), Validation of
Analytical Procedures Methodology, Geneva, Switzerland,
November .1996.
@article{"International Journal of Medical, Medicine and Health Sciences:60191", author = "So-young Jung and Bu-chuhl Choe and Gi-young Shin and Jung-hun Kim and Young-zoo Chae", title = "Analysis of Roasted and Ground Grains on the Seoul (Korea) Market for Their Contaminants of Aflatoxins, Ochratoxin A and Fusarium Toxins by LC-MS/MS", abstract = "A sensitive and specific method for quantitative
determination of aflatoxins(B1, B2, G1,G2), deoxynivalenol,
fumonisin(B1,B2), ochratoxin A, zearalenone, T-2 and HT-2 in
roasted and ground grains using liquid chromatography combined
with tandem mass spectrometry. A double extraction using a
phosphate buffer solution followed by methanol was applied to
achieve effective co extraction of 11 mycotoxins. A multitoxin
immunoaffinity column for all these mycotoxins was used to clean up
the extract. The LODs of mycotoxins were 0.1~6.1 μg/kg, LOQs were
0.3~18.4 μg/kg. Forty seven samples collected from Seoul (Korea) for
mycotoxin contamination monitoring. The results showed that the
occurrence of zearalenone and deoxynivalenol were frequent.
Zearalenone was detected in all samples and deoxynivalenol was
detected in 80.9 % samples in the range 0.626 ~ 29.264 μg/kg and N.D
~ 48.332 μg/kg respectively. Fumonisins and ochratoxin A were
detected in 46.8% samples and 17 % samples respectively, aflatoxins
and T-2/HT-2 toxins were not detected all samples.", keywords = "LC-MS/MS, mycotoxins, roasted and ground grains.", volume = "6", number = "12", pages = "702-4", }
