The objective of this research was to determine the
potency of indigenous acid-aluminium tolerant Bradyrhizobium
japonicum as producer of indole acetic acid (IAA) and applied it as
nitrogen fixation on local soybeans viz Anjasmoro, Tanggamus
(yellow soybean seeds), and Detam (black soybean seed). Three
isolates of acid-aluminium tolerant Bradyrhizobium japonicum (BJ)
were used in this research, i.e. BJ 11 (wt), BJ 11 (19) - BJ 11(wt)
mutant, and USDA 110 as a reference isolate. All of isolates tested to
produce the IAA by using Salkowsky method. Effect of IAA
production by each of B. japonicum was tested on growth pouch and
greenhouse using three varieties of soybean. All isolates could grow
well and produce IAA on yeast mannitol broth (YMB) medium in
the presence of 0.5 mM L-tryptophan. BJ 11 (19) produced the
highest of IAA at 4 days incubation compared to BJ 11 (wt) and
USDA 110. All tested isolates of Bradyrhizobium japonicum have
showed effect on stimulating the formation of root nodules in
soybean varieties grown on Leonard bottle. The concentration of
IAA on root nodules of soybean symbiotic with B. japonicum was
significantly different with control, except on the treatment using
Tanggamus soybean.
[1] T. Endarini, A.T. Wahyudi, and Tedja-Imas, "Selection of indigenous
Bradyrhizobium japonicum tolerant acid-aluminium medium" (in
Indonesia language), Hayati, vol. 2, pp. 74-79, 1995.
[2] J. Denarie, P. Debelle, and C. Rosenberg, "Signaling and host range
variation in nodulation, " Ann. Rev. Microbiol.,vol. 46, pp.497-531,
1992.
[3] N.R. Mubarik, T. Imas, A.T. Wahyud, Triadiati, Suharyanto, and H.
Widiastuti, --The use of acid-alumunium tolerant Bradyrhizobium
japonicum formula," World Acad. Sci. Eng .Technol., vol. 53, pp. 879-
882, 2011.
[4] A.T. Wahyudi, A. Suwanto, Tedja-Imas and A. Tjahyoleksono,
"Screening of acid-aluminium tolerant Bradyrhizobium japonicum strain
analysis of marker genes and competition in planta," Aspac. J. Mol. Biol.
Biotechnol . vol. 6, pp. 13-20, 1998.
[5] A.R.F. Situmorang, N.R. Mubarik, and Triadiati, "The use of inoculant
acid- aluminium tolerant Bradyrhizobium japonicum for soybean
growth on acid soils," Hayati J. Biosci., vol. 16, pp. 157-160, 2009.
[6] N.R. Mubarik, H. Habibah, and A.T.Wahyudi , "Greenhouse
experiments of symbiotic effectiveness of acid-aluminium tolerance
Bradyrhizobium japonicum on soybean plant",in. International
Conference on Applied Life Sciences. F. Nejadkoorki F, Ed.Rijeka,
Croatia: InTech. 2012.pp. 337-342. DOI:10.5772/52498.
[7] J.J. Fuhrmann, "Population diversity grouping of soybean
bradyrhizobia", in Advances in Agronomy Vol 50. New York:
Academic Press,1993, pp. 67-105.
[8] C.Patten and B.R. Glick, . 1996. "Bacterial biosynthesis of indole-3-
acetic acid," Can. J. Microbiol. ,vol. 42, pp. 207-220, 1996.
[9] A. Karnwal, "Production of indole acetic acid by fluorescent
Pseudomonas in the presence of L-tryptophan and rice root exudates,".
J. Plant. Pathol., vol. 9, pp. 61-63, 2009.
[10] S. Unyayar, S.F. Topcuoglu, and A. Unyayar. "A modified method for
extraction and identification of indole-3-acetic acid (IAA), gibberellic
acid (GA3), abscisic acid (ABA), and zeatin produced by
Phanerochaete chrysosporium ME446," [short communication], Bulg.
J. Plant. Physiol., vol. 22, pp. 105-110, 1996.
[11] W.J. Hunter, "Increased nodulation of soybean by a strain of
Bradyrhizobium japonicum with altered tryptophan metabolism," Lett.
Appl .Microbiol., vol. 18, pp. 340-342, 1994.
[12] E. Husen, A.T. Wahyudi, A. Suwanto, R. Saraswati, "Prospective use of
1-aminocyclopropane-1-carboxylate deaminase-producing bacteria for
plant growth promotion and defense against biotic and abiotic stresses in
peat-soil-agriculture," Microbiol. Indones., vol. 2, pp. 107-111, 2008.
[1] T. Endarini, A.T. Wahyudi, and Tedja-Imas, "Selection of indigenous
Bradyrhizobium japonicum tolerant acid-aluminium medium" (in
Indonesia language), Hayati, vol. 2, pp. 74-79, 1995.
[2] J. Denarie, P. Debelle, and C. Rosenberg, "Signaling and host range
variation in nodulation, " Ann. Rev. Microbiol.,vol. 46, pp.497-531,
1992.
[3] N.R. Mubarik, T. Imas, A.T. Wahyud, Triadiati, Suharyanto, and H.
Widiastuti, --The use of acid-alumunium tolerant Bradyrhizobium
japonicum formula," World Acad. Sci. Eng .Technol., vol. 53, pp. 879-
882, 2011.
[4] A.T. Wahyudi, A. Suwanto, Tedja-Imas and A. Tjahyoleksono,
"Screening of acid-aluminium tolerant Bradyrhizobium japonicum strain
analysis of marker genes and competition in planta," Aspac. J. Mol. Biol.
Biotechnol . vol. 6, pp. 13-20, 1998.
[5] A.R.F. Situmorang, N.R. Mubarik, and Triadiati, "The use of inoculant
acid- aluminium tolerant Bradyrhizobium japonicum for soybean
growth on acid soils," Hayati J. Biosci., vol. 16, pp. 157-160, 2009.
[6] N.R. Mubarik, H. Habibah, and A.T.Wahyudi , "Greenhouse
experiments of symbiotic effectiveness of acid-aluminium tolerance
Bradyrhizobium japonicum on soybean plant",in. International
Conference on Applied Life Sciences. F. Nejadkoorki F, Ed.Rijeka,
Croatia: InTech. 2012.pp. 337-342. DOI:10.5772/52498.
[7] J.J. Fuhrmann, "Population diversity grouping of soybean
bradyrhizobia", in Advances in Agronomy Vol 50. New York:
Academic Press,1993, pp. 67-105.
[8] C.Patten and B.R. Glick, . 1996. "Bacterial biosynthesis of indole-3-
acetic acid," Can. J. Microbiol. ,vol. 42, pp. 207-220, 1996.
[9] A. Karnwal, "Production of indole acetic acid by fluorescent
Pseudomonas in the presence of L-tryptophan and rice root exudates,".
J. Plant. Pathol., vol. 9, pp. 61-63, 2009.
[10] S. Unyayar, S.F. Topcuoglu, and A. Unyayar. "A modified method for
extraction and identification of indole-3-acetic acid (IAA), gibberellic
acid (GA3), abscisic acid (ABA), and zeatin produced by
Phanerochaete chrysosporium ME446," [short communication], Bulg.
J. Plant. Physiol., vol. 22, pp. 105-110, 1996.
[11] W.J. Hunter, "Increased nodulation of soybean by a strain of
Bradyrhizobium japonicum with altered tryptophan metabolism," Lett.
Appl .Microbiol., vol. 18, pp. 340-342, 1994.
[12] E. Husen, A.T. Wahyudi, A. Suwanto, R. Saraswati, "Prospective use of
1-aminocyclopropane-1-carboxylate deaminase-producing bacteria for
plant growth promotion and defense against biotic and abiotic stresses in
peat-soil-agriculture," Microbiol. Indones., vol. 2, pp. 107-111, 2008.
@article{"International Journal of Biological, Life and Agricultural Sciences:57199", author = "Nisa Rachmania Mubarik and Irni Mahagiani and Aris Tri Wahyudi", title = "Production of IAA by Bradyrhizobium sp.", abstract = "The objective of this research was to determine the
potency of indigenous acid-aluminium tolerant Bradyrhizobium
japonicum as producer of indole acetic acid (IAA) and applied it as
nitrogen fixation on local soybeans viz Anjasmoro, Tanggamus
(yellow soybean seeds), and Detam (black soybean seed). Three
isolates of acid-aluminium tolerant Bradyrhizobium japonicum (BJ)
were used in this research, i.e. BJ 11 (wt), BJ 11 (19) - BJ 11(wt)
mutant, and USDA 110 as a reference isolate. All of isolates tested to
produce the IAA by using Salkowsky method. Effect of IAA
production by each of B. japonicum was tested on growth pouch and
greenhouse using three varieties of soybean. All isolates could grow
well and produce IAA on yeast mannitol broth (YMB) medium in
the presence of 0.5 mM L-tryptophan. BJ 11 (19) produced the
highest of IAA at 4 days incubation compared to BJ 11 (wt) and
USDA 110. All tested isolates of Bradyrhizobium japonicum have
showed effect on stimulating the formation of root nodules in
soybean varieties grown on Leonard bottle. The concentration of
IAA on root nodules of soybean symbiotic with B. japonicum was
significantly different with control, except on the treatment using
Tanggamus soybean.", keywords = "Acid-aluminium tolerant isolate, Bradyrhizobium
japonicum, indole acetic acid, soybean.", volume = "7", number = "2", pages = "107-4", }