Abstract: A bio-sensing method, based on the plasmonic property of gold nano-islands, has been developed for detection of exosomes in a clinical setting. The position of the gold plasmon band in the UV-Visible spectrum depends on the size and shape of gold nanoparticles as well as on the surrounding environment. By adsorbing various chemical entities, or binding them, the gold plasmon band will shift toward longer wavelengths and the shift is proportional to the concentration. Exosomes transport cargoes of molecules and genetic materials to proximal and distal cells. Presently, the standard method for their isolation and quantification from body fluids is by ultracentrifugation, not a practical method to be implemented in a clinical setting. Thus, a versatile and cutting-edge platform is required to selectively detect and isolate exosomes for further analysis at clinical level. The new sensing protocol, instead of antibodies, makes use of a specially synthesized polypeptide (Vn96), to capture and quantify the exosomes from different media, by binding the heat shock proteins from exosomes. The protocol has been established and optimized by using a glass substrate, in order to facilitate the next stage, namely the transfer of the protocol to a microfluidic environment. After each step of the protocol, the UV-Vis spectrum was recorded and the position of gold Localized Surface Plasmon Resonance (LSPR) band was measured. The sensing process was modelled, taking into account the characteristics of the nano-island structure, prepared by thermal convection and annealing. The optimal molar ratios of the most important chemical entities, involved in the detection of exosomes were calculated as well. Indeed, it was found that the results of the sensing process depend on the two major steps: the molar ratios of streptavidin to biotin-PEG-Vn96 and, the final step, the capture of exosomes by the biotin-PEG-Vn96 complex. The microfluidic device designed for sensing of exosomes consists of a glass substrate, sealed by a PDMS layer that contains the channel and a collecting chamber. In the device, the solutions of linker, cross-linker, etc., are pumped over the gold nano-islands and an Ocean Optics spectrometer is used to measure the position of the Au plasmon band at each step of the sensing. The experiments have shown that the shift of the Au LSPR band is proportional to the concentration of exosomes and, thereby, exosomes can be accurately quantified. An important advantage of the method is the ability to discriminate between exosomes having different origins.
Abstract: The problem of thermal convection in temperature and
magnetic field sensitive Newtonian ferromagnetic liquid is studied
in the presence of uniform vertical magnetic field and throughflow.
Using a combination of Galerkin and shooting techniques the critical
eigenvalues are obtained for stationary mode. The effect of Prandtl
number (Pr > 1) on onset is insignificant and nonlinearity of
non-buoyancy magnetic parameter M3 is found to have no influence
on the onset of ferroconvection. The magnetic buoyancy number, M1
and variable viscosity parameter, V have destabilizing influences on
the system. The effect of throughflow Peclet number, Pe is to delay
the onset of ferroconvection and this effect is independent of the
direction of flow.
Abstract: We investigate properties of convective solutions of the
Boussinesq thermal convection in a moderately rotating spherical
shell allowing the inner and outer sphere rotation due to the viscous
torque of the fluid. The ratio of the inner and outer radii of the
spheres, the Prandtl number and the Taylor number are fixed to 0.4,
1 and 5002, respectively. The inertial moments of the inner and outer
spheres are fixed to about 0.22 and 100, respectively. The Rayleigh
number is varied from 2.6 × 104 to 3.4 × 104. In this parameter
range, convective solutions transit from equatorially symmetric quasiperiodic
ones to equatorially asymmetric chaotic ones as the Rayleigh
number is increased. The transition route in the system allowing
rotation of both the spheres is different from that in the co-rotating
system, which means the inner and outer spheres rotate with the
same constant angular velocity: the convective solutions transit as
equatorially symmetric quasi-periodic solution → equatorially symmetric
chaotic solution → equatorially asymmetric chaotic solution
in the system allowing both the spheres rotation, while equatorially
symmetric quasi-periodic solution → equatorially asymmetric quasiperiodic
solution → equatorially asymmetric chaotic solution in the
co-rotating system.
Abstract: This work examines thermal convection in two porous
layers. Flow in the upper layer is governed by Brinkman-s equations
model and in the lower layer is governed by Darcy-s model.
Legendre polynomials are used to obtain numerical solution when the
lower layer is heated from below.